Click here to switch to the map view.

The map label for this gene is lepA [H]

Identifier: 99080519

GI number: 99080519

Start: 724251

End: 726050

Strand: Direct

Name: lepA [H]

Synonym: TM1040_0678

Alternate gene names: 99080519

Gene position: 724251-726050 (Clockwise)

Preceding gene: 99080516

Following gene: 99080520

Centisome position: 22.63

GC content: 58.56

Gene sequence:

>1800_bases
ATGACAGAGCTCTCGAAAATCCGCAATTTTTCCATCGTCGCCCATATCGACCATGGTAAGTCCACGCTTGCAGACCGCCT
CATCCAGTCGACGGGGACGGTGCAGGATCGTGACATGAAAGAGCAGTTGCTCGACGCCATGGACATCGAGCGTGAACGGG
GCATCACCATCAAGGCGAACACCGTCCGCATCGACTACGAAGCCGATGACGGGGAACGCTATGTGCTGAACCTGATCGAC
ACCCCCGGCCACGTCGACTTTGCCTATGAGGTGTCGCGGTCCATGCGCGCGGTTGAGGGCTCTTTGCTCGTTGTCGACTC
CACCCAAGGGGTCGAGGCGCAGACCTTGGCGAATGTCTACCAGGCGATTGATGCGGACCACGAGATCGTTCCGGTTCTGA
ACAAGATCGACTTGCCGGCGGCCGATTGTGACCGTGTGGCAGAGCAGATCGAGGACGTGATCGGCATCGATGCAACCGAT
GCGATCCGAGTCTCGGCCAAAACCGGCATCGGCATCAAGGACACGCTTGAGGCCATCGTGAAACGTCTGCCCGCCCCGAC
AGGAGAGCGCAACGCGCCTCTGAAGGCGATGCTGGTGGACAGCTGGTACGACGCTTATCTGGGCGTGATCGTTCTGGTGC
GCATCATGGACGGGGTCCTGAAGAAGGGCGACCAGATCAAGATGATGCAGACCAACGCGCGGTACGGTGTGGACCGGATC
GGTGTATTCCGCCCTGCGATGCAGGTGGTGGACGAGCTTGGGCCGGGCGAGATCGGCTTTATCACTGCTTCGATCAAACA
GGTGCGTGACACCAAGGTGGGCGATACCATCACCCACGACAAGAAAGGCGCAGAGACCGCATTGCCGGGCTTTAAGCCCT
CTGTGCCCGTGGTGTTCTGTGGCCTGTTCCCGGTGGACAGCTCGGAATTCGAGGACCTGCGCGACGCGATTGAAAAGCTG
GCGCTCAATGATGCGTCCTTCTCCTACGAGATGGAAACCTCCGCCGCGCTTGGGTTTGGTTTCCGTTGCGGCTTCCTTGG
GCTTTTGCACCTTGAGGTAATCCGGGACCGGATCGAGCGAGAGTATAATATCGAGCTGATCACCACGGCACCGTCGGTTG
TCTATCATATCTTCATGAAAGACGGGGAGATGCTTGAACTGCATAACCCGGCTGACATGCCCGACCTGTCCAAGGTGGAT
CATCTCGAAGAGCCGCGCATCAAGGCGACCATCCTGGTGCCGGATGAGTATCTCGGTGACGTGCTCAAGCTGTGTCAGGA
CCGCCGCGGCATTCAGCAGGACCTGTCCTATGCGGGCTCGCGGGCCATGGTGGTCTACGACCTGCCGCTCAACGAGGTGG
TGTTTGACTTCTATGATCGTCTGAAATCGGTCACGAAAGGCTATGCGTCCTTTGATTACCAGATGATCGGCTATCGCGCG
GACAATCTCGTAAAGATGTCGGTGCTGGTGAATGACGAACCTGTGGATGCGCTCTCGACCATGGTTCATCGCGACCGTGC
CGAGCAGCGGGGCCGCGCCATGTGTGAAAAGCTCAAGGACCTGATCCCGCGCCACATGTTCAAGATTCCGATCCAGGCCG
CGATTGGTGGCAAGGTGATCGCGCGCGAGACGCTCTCGGCCCTGCGCAAGGATGTGACCGCAAAATGTTACGGCGGCGAC
GCCACCCGAAAACGCAAGCTCCTTGAAAAGCAAAAAGCCGGTAAGAAGAAGATGCGCCAGTTCGGCCGGGTTGAAATCCC
GCAAGAGGCGTTTATCTCCGCGCTCAAGATGGACGACTGA

Upstream 100 bases:

>100_bases
GCGCCAGCGCGGCGACCGGTCGCATCTTCGCGCCAATCCGGTGGGCAGTTGCGCATGATCCACCTTGACCAAAGGCGAAA
TCCCAATCATATGCGCATGC

Downstream 100 bases:

>100_bases
ACAAAAGGCCTCCCCTCGCGGGAGGTCTTTTTGATTGAGAGAGGGGGCTGGAAACAGCGGTCATCGCGCCAGCCCTGCTT
GCATTTCGGTCCGACGCAGG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTELSKIRNFSIVAHIDHGKSTLADRLIQSTGTVQDRDMKEQLLDAMDIERERGITIKANTVRIDYEADDGERYVLNLID
TPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVYQAIDADHEIVPVLNKIDLPAADCDRVAEQIEDVIGIDATD
AIRVSAKTGIGIKDTLEAIVKRLPAPTGERNAPLKAMLVDSWYDAYLGVIVLVRIMDGVLKKGDQIKMMQTNARYGVDRI
GVFRPAMQVVDELGPGEIGFITASIKQVRDTKVGDTITHDKKGAETALPGFKPSVPVVFCGLFPVDSSEFEDLRDAIEKL
ALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIEREYNIELITTAPSVVYHIFMKDGEMLELHNPADMPDLSKVD
HLEEPRIKATILVPDEYLGDVLKLCQDRRGIQQDLSYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYQMIGYRA
DNLVKMSVLVNDEPVDALSTMVHRDRAEQRGRAMCEKLKDLIPRHMFKIPIQAAIGGKVIARETLSALRKDVTAKCYGGD
ATRKRKLLEKQKAGKKKMRQFGRVEIPQEAFISALKMDD

Sequences:

>Translated_599_residues
MTELSKIRNFSIVAHIDHGKSTLADRLIQSTGTVQDRDMKEQLLDAMDIERERGITIKANTVRIDYEADDGERYVLNLID
TPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVYQAIDADHEIVPVLNKIDLPAADCDRVAEQIEDVIGIDATD
AIRVSAKTGIGIKDTLEAIVKRLPAPTGERNAPLKAMLVDSWYDAYLGVIVLVRIMDGVLKKGDQIKMMQTNARYGVDRI
GVFRPAMQVVDELGPGEIGFITASIKQVRDTKVGDTITHDKKGAETALPGFKPSVPVVFCGLFPVDSSEFEDLRDAIEKL
ALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIEREYNIELITTAPSVVYHIFMKDGEMLELHNPADMPDLSKVD
HLEEPRIKATILVPDEYLGDVLKLCQDRRGIQQDLSYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYQMIGYRA
DNLVKMSVLVNDEPVDALSTMVHRDRAEQRGRAMCEKLKDLIPRHMFKIPIQAAIGGKVIARETLSALRKDVTAKCYGGD
ATRKRKLLEKQKAGKKKMRQFGRVEIPQEAFISALKMDD
>Mature_598_residues
TELSKIRNFSIVAHIDHGKSTLADRLIQSTGTVQDRDMKEQLLDAMDIERERGITIKANTVRIDYEADDGERYVLNLIDT
PGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVYQAIDADHEIVPVLNKIDLPAADCDRVAEQIEDVIGIDATDA
IRVSAKTGIGIKDTLEAIVKRLPAPTGERNAPLKAMLVDSWYDAYLGVIVLVRIMDGVLKKGDQIKMMQTNARYGVDRIG
VFRPAMQVVDELGPGEIGFITASIKQVRDTKVGDTITHDKKGAETALPGFKPSVPVVFCGLFPVDSSEFEDLRDAIEKLA
LNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIEREYNIELITTAPSVVYHIFMKDGEMLELHNPADMPDLSKVDH
LEEPRIKATILVPDEYLGDVLKLCQDRRGIQQDLSYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYQMIGYRAD
NLVKMSVLVNDEPVDALSTMVHRDRAEQRGRAMCEKLKDLIPRHMFKIPIQAAIGGKVIARETLSALRKDVTAKCYGGDA
TRKRKLLEKQKAGKKKMRQFGRVEIPQEAFISALKMDD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=49.6677740863787, Blast_Score=624, Evalue=1e-179,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=45.5223880597015, Blast_Score=116, Evalue=8e-26,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=39.5973154362416, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=41.4473684210526, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=41.4473684210526, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=41.4473684210526, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI18390331, Length=159, Percent_Identity=34.5911949685535, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=42.3423423423423, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=42.3423423423423, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=42.3423423423423, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI217272894, Length=168, Percent_Identity=31.547619047619, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272892, Length=168, Percent_Identity=31.547619047619, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI53729339, Length=220, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=220, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=56.3973063973064, Blast_Score=699, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=513, Percent_Identity=28.8499025341131, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789738, Length=213, Percent_Identity=32.3943661971831, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1790835, Length=163, Percent_Identity=31.2883435582822, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789559, Length=256, Percent_Identity=30.078125, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=614, Percent_Identity=40.0651465798046, Blast_Score=476, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17556745, Length=157, Percent_Identity=38.2165605095541, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17506493, Length=169, Percent_Identity=34.9112426035503, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=144, Percent_Identity=36.1111111111111, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=132, Percent_Identity=37.1212121212121, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=37.1212121212121, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=34.4827586206897, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=45.0166112956811, Blast_Score=531, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=39.5973154362416, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=39.5973154362416, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.2637362637363, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=139, Percent_Identity=35.9712230215827, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=172, Percent_Identity=36.046511627907, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=261, Percent_Identity=26.4367816091954, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.5182724252492, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI28574573, Length=196, Percent_Identity=38.7755102040816, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582462, Length=183, Percent_Identity=32.7868852459016, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=35.9477124183007, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=35.9477124183007, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=35.9477124183007, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21357743, Length=165, Percent_Identity=33.9393939393939, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221458488, Length=156, Percent_Identity=40.3846153846154, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281363316, Length=286, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17864358, Length=286, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66852; Mature: 66721

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELSKIRNFSIVAHIDHGKSTLADRLIQSTGTVQDRDMKEQLLDAMDIERERGITIKAN
CCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
TVRIDYEADDGERYVLNLIDTPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVY
EEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
QAIDADHEIVPVLNKIDLPAADCDRVAEQIEDVIGIDATDAIRVSAKTGIGIKDTLEAIV
HHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHH
KRLPAPTGERNAPLKAMLVDSWYDAYLGVIVLVRIMDGVLKKGDQIKMMQTNARYGVDRI
HHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
GVFRPAMQVVDELGPGEIGFITASIKQVRDTKVGDTITHDKKGAETALPGFKPSVPVVFC
HHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
GLFPVDSSEFEDLRDAIEKLALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIER
ECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCC
EYNIELITTAPSVVYHIFMKDGEMLELHNPADMPDLSKVDHLEEPRIKATILVPDEYLGD
CCCEEEEECCHHHEEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCEEEEEECCHHHHHH
VLKLCQDRRGIQQDLSYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYQMIGYRA
HHHHHHHHCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
DNLVKMSVLVNDEPVDALSTMVHRDRAEQRGRAMCEKLKDLIPRHMFKIPIQAAIGGKVI
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHH
ARETLSALRKDVTAKCYGGDATRKRKLLEKQKAGKKKMRQFGRVEIPQEAFISALKMDD
HHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TELSKIRNFSIVAHIDHGKSTLADRLIQSTGTVQDRDMKEQLLDAMDIERERGITIKAN
CCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
TVRIDYEADDGERYVLNLIDTPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVY
EEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
QAIDADHEIVPVLNKIDLPAADCDRVAEQIEDVIGIDATDAIRVSAKTGIGIKDTLEAIV
HHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHH
KRLPAPTGERNAPLKAMLVDSWYDAYLGVIVLVRIMDGVLKKGDQIKMMQTNARYGVDRI
HHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
GVFRPAMQVVDELGPGEIGFITASIKQVRDTKVGDTITHDKKGAETALPGFKPSVPVVFC
HHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
GLFPVDSSEFEDLRDAIEKLALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIER
ECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCC
EYNIELITTAPSVVYHIFMKDGEMLELHNPADMPDLSKVDHLEEPRIKATILVPDEYLGD
CCCEEEEECCHHHEEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCEEEEEECCHHHHHH
VLKLCQDRRGIQQDLSYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYQMIGYRA
HHHHHHHHCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
DNLVKMSVLVNDEPVDALSTMVHRDRAEQRGRAMCEKLKDLIPRHMFKIPIQAAIGGKVI
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHH
ARETLSALRKDVTAKCYGGDATRKRKLLEKQKAGKKKMRQFGRVEIPQEAFISALKMDD
HHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA