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The map label for this gene is murD

Identifier: 99080520

GI number: 99080520

Start: 726193

End: 727596

Strand: Direct

Name: murD

Synonym: TM1040_0679

Alternate gene names: 99080520

Gene position: 726193-727596 (Clockwise)

Preceding gene: 99080519

Following gene: 99080522

Centisome position: 22.69

GC content: 63.39

Gene sequence:

>1404_bases
ATGATCCCGGTCAAAGGTGTCGCAGGGCAAAACGTGGCTGTGCTGGGGCTTGGGCGCTCGGGGCTCTCTGCGGCGCGGGC
CTTGCGCGAAGGCGGGGCAGAGGCGATCTGTTGGGACGATAACCCCGCTGCACGCGCCACTGCCGAGGCAGAGGGCTTCA
CCTGTCGTGAATTGACCAAGCAGGGCGCATTCGATGGCATCTCCGCCCTCATCGTGTCGCCCGGGATTGCGCATCTTTAC
CCGAAACCCAATCCGGTTATCCGGGCTGCCCTGGTGGCGGGTGTGCCGGTGGATAATGACATTGGCCTGTTCTTCCGCTC
TGTTGCGACCTCGGAGTGGGACGAGTTTGACACCGCGCCGAAGGTGATTGCCGTAACGGGCTCAAACGGCAAATCGACGA
CGGTTGCCTTGCTGCACCACATTCTTGAACATGTCGGGCGCAACAGTCAGATGGCGGGCAATATCGGGCGCGGGGTGCTG
GACATCGACCCACCCGTGGACGGTGGTGTCGTGGTGCTGGAGCTCTCCTCCTATCAAACCGATCTGGCGCGCGCGCTCAC
ACCGGATGTCGCGGTCTTCACCAATCTGACGCCGGATCATCTGGACCGTCACGCTGGAATGGGGGGGTATTTTGCGGCCA
AGCGGCGCCTTTTTGCAGAGGGTGGCCCGGATCGCGCGGTCATCGGAGTTGATGAGGACGAGGGGCTTTTCCTTGCAGGG
CAACTGAGCGAAGGCGAGAGCGATGATCGCGTGATCCGCATCTCAGCGCGCCAGAAGCTCTCGGGCGGGGCAGGGTGGAC
CGTCTTTGCTCGCAAGGGGTTCTTGTCGGAGTATCGCAAGGGGCGGCAGGCCGGCTCGATTGATCTTCGCGACATTCCCG
GTCTGCCCGGAGCGCATAACCATCAGAACGCCTGCGCGGCCTACGCTGCAGCCCGTACGCTTGGGCTGGCGCCGCGGGTG
ATCGAGGCGGCGATGCGGACCTATCCGGGGCTGCCGCACCGCAGCCAGCGCATCGCGGAGGCGGATGGGGTATCTTATGT
CAATGACAGCAAGGCCACCAACGTCGACAGCGCCGCCAAGGCGCTGAGCGCGTTCAAAAATATCCGCTGGATCTGCGGCG
GCTTGGAAAAGGAAGGCGGGCTTGAGGCGCTGAAAGGTGCCACGGATGATGTGCTCAAGGCCTATGTGATCGGCCGCGAG
GCGGCGCAATTTGCCATGCAGCTCGATGTGGAGGCCGAGGTCTGCACCACCATGGCGCAAGCTGTCTCGCGTGCCATGGC
CGAGGCCGGCCCGGGGGAGACAGTGCTTCTGGCCCCTGCGGCGGCGAGCTTTGATCAATACGACAGTTTTGAAAAACGCG
GTGAGGATTTTATCGCTGAGGTCACGGCGCGCCTGAACGGCTAG

Upstream 100 bases:

>100_bases
GGGGGCTGGAAACAGCGGTCATCGCGCCAGCCCTGCTTGCATTTCGGTCCGACGCAGGTGCAATGTCGCGCCGACTTAGA
TTTCCAGAACGAGGTGAAGC

Downstream 100 bases:

>100_bases
ACGTCTCAGCCCCGAATGGCGGTGCCTGCGGCGTGGCCCGACGCCCAGGCCCACTGGAAGTTATAGCCGCCGAGCCAGCC
GGTCACGTCCACCGCTTCGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIPVKGVAGQNVAVLGLGRSGLSAARALREGGAEAICWDDNPAARATAEAEGFTCRELTKQGAFDGISALIVSPGIAHLY
PKPNPVIRAALVAGVPVDNDIGLFFRSVATSEWDEFDTAPKVIAVTGSNGKSTTVALLHHILEHVGRNSQMAGNIGRGVL
DIDPPVDGGVVVLELSSYQTDLARALTPDVAVFTNLTPDHLDRHAGMGGYFAAKRRLFAEGGPDRAVIGVDEDEGLFLAG
QLSEGESDDRVIRISARQKLSGGAGWTVFARKGFLSEYRKGRQAGSIDLRDIPGLPGAHNHQNACAAYAAARTLGLAPRV
IEAAMRTYPGLPHRSQRIAEADGVSYVNDSKATNVDSAAKALSAFKNIRWICGGLEKEGGLEALKGATDDVLKAYVIGRE
AAQFAMQLDVEAEVCTTMAQAVSRAMAEAGPGETVLLAPAAASFDQYDSFEKRGEDFIAEVTARLNG

Sequences:

>Translated_467_residues
MIPVKGVAGQNVAVLGLGRSGLSAARALREGGAEAICWDDNPAARATAEAEGFTCRELTKQGAFDGISALIVSPGIAHLY
PKPNPVIRAALVAGVPVDNDIGLFFRSVATSEWDEFDTAPKVIAVTGSNGKSTTVALLHHILEHVGRNSQMAGNIGRGVL
DIDPPVDGGVVVLELSSYQTDLARALTPDVAVFTNLTPDHLDRHAGMGGYFAAKRRLFAEGGPDRAVIGVDEDEGLFLAG
QLSEGESDDRVIRISARQKLSGGAGWTVFARKGFLSEYRKGRQAGSIDLRDIPGLPGAHNHQNACAAYAAARTLGLAPRV
IEAAMRTYPGLPHRSQRIAEADGVSYVNDSKATNVDSAAKALSAFKNIRWICGGLEKEGGLEALKGATDDVLKAYVIGRE
AAQFAMQLDVEAEVCTTMAQAVSRAMAEAGPGETVLLAPAAASFDQYDSFEKRGEDFIAEVTARLNG
>Mature_467_residues
MIPVKGVAGQNVAVLGLGRSGLSAARALREGGAEAICWDDNPAARATAEAEGFTCRELTKQGAFDGISALIVSPGIAHLY
PKPNPVIRAALVAGVPVDNDIGLFFRSVATSEWDEFDTAPKVIAVTGSNGKSTTVALLHHILEHVGRNSQMAGNIGRGVL
DIDPPVDGGVVVLELSSYQTDLARALTPDVAVFTNLTPDHLDRHAGMGGYFAAKRRLFAEGGPDRAVIGVDEDEGLFLAG
QLSEGESDDRVIRISARQKLSGGAGWTVFARKGFLSEYRKGRQAGSIDLRDIPGLPGAHNHQNACAAYAAARTLGLAPRV
IEAAMRTYPGLPHRSQRIAEADGVSYVNDSKATNVDSAAKALSAFKNIRWICGGLEKEGGLEALKGATDDVLKAYVIGRE
AAQFAMQLDVEAEVCTTMAQAVSRAMAEAGPGETVLLAPAAASFDQYDSFEKRGEDFIAEVTARLNG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=461, Percent_Identity=31.8872017353579, Blast_Score=158, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_SILST (Q1GIV4)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612674.1
- ProteinModelPortal:   Q1GIV4
- STRING:   Q1GIV4
- GeneID:   4077287
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0679
- NMPDR:   fig|292414.1.peg.2785
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- PhylomeDB:   Q1GIV4
- ProtClustDB:   PRK01390
- BioCyc:   SSP292414:TM1040_0679-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49149; Mature: 49149

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVKGVAGQNVAVLGLGRSGLSAARALREGGAEAICWDDNPAARATAEAEGFTCRELTK
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
QGAFDGISALIVSPGIAHLYPKPNPVIRAALVAGVPVDNDIGLFFRSVATSEWDEFDTAP
CCCCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCCC
KVIAVTGSNGKSTTVALLHHILEHVGRNSQMAGNIGRGVLDIDPPVDGGVVVLELSSYQT
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCHHH
DLARALTPDVAVFTNLTPDHLDRHAGMGGYFAAKRRLFAEGGPDRAVIGVDEDEGLFLAG
HHHHHHCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
QLSEGESDDRVIRISARQKLSGGAGWTVFARKGFLSEYRKGRQAGSIDLRDIPGLPGAHN
ECCCCCCCCCEEEEECHHCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCC
HQNACAAYAAARTLGLAPRVIEAAMRTYPGLPHRSQRIAEADGVSYVNDSKATNVDSAAK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHH
ALSAFKNIRWICGGLEKEGGLEALKGATDDVLKAYVIGREAAQFAMQLDVEAEVCTTMAQ
HHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AVSRAMAEAGPGETVLLAPAAASFDQYDSFEKRGEDFIAEVTARLNG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPVKGVAGQNVAVLGLGRSGLSAARALREGGAEAICWDDNPAARATAEAEGFTCRELTK
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
QGAFDGISALIVSPGIAHLYPKPNPVIRAALVAGVPVDNDIGLFFRSVATSEWDEFDTAP
CCCCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCCC
KVIAVTGSNGKSTTVALLHHILEHVGRNSQMAGNIGRGVLDIDPPVDGGVVVLELSSYQT
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCHHH
DLARALTPDVAVFTNLTPDHLDRHAGMGGYFAAKRRLFAEGGPDRAVIGVDEDEGLFLAG
HHHHHHCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
QLSEGESDDRVIRISARQKLSGGAGWTVFARKGFLSEYRKGRQAGSIDLRDIPGLPGAHN
ECCCCCCCCCEEEEECHHCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCC
HQNACAAYAAARTLGLAPRVIEAAMRTYPGLPHRSQRIAEADGVSYVNDSKATNVDSAAK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHH
ALSAFKNIRWICGGLEKEGGLEALKGATDDVLKAYVIGREAAQFAMQLDVEAEVCTTMAQ
HHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AVSRAMAEAGPGETVLLAPAAASFDQYDSFEKRGEDFIAEVTARLNG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA