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The map label for this gene is purK [H]

Identifier: 99080444

GI number: 99080444

Start: 643704

End: 644801

Strand: Reverse

Name: purK [H]

Synonym: TM1040_0603

Alternate gene names: 99080444

Gene position: 644801-643704 (Counterclockwise)

Preceding gene: 99080445

Following gene: 99080435

Centisome position: 20.14

GC content: 60.11

Gene sequence:

>1098_bases
ATGACTGATCTCGACCAAATCCTCGAACCCGGTGCCGTAATCGGCATTCTGGGCGGTGGTCAGCTTGGCCGGATGCTGTC
GGTTGCAGCCTCCCGCCTTGGCTTCACCACCCACATCTACGAGCCGGCCGCGAATCCGCCTGCGGGCCATGTTGCGGATC
GGGTCACAACCGCTGCCTATGAGGATGAGGCGGCCTTGCGCGCCTTTGCACAAACCGTGGATGTGATCACCTATGAGTTT
GAAAATATCCCCACCTCAGCGCTCGACATTCTCGAGGATCTGAAACCGATCCGCCCCGGTCGCGAGGCGCTTCGGGTGTC
GCAGGACCGCCTGACGGAAAAGACCTTTCTGCAAGAGCTTGGGCTGGCAACAGCGCCCTTTGCAGAGGTGGATGATGCCG
CCAGCCTTGAAGTTGCATTGGAAAAGGTCGGCACGCCTTCGATCCTGAAAACGCGGCGTTTTGGCTATGATGGCAAGGGT
CAGATGCGCATCATGCATCCCGAAGAAGCTCCGGAGGCCTTGGCCGCCATGCAAGGAGCGCCCGCGGTGCTCGAAGGGTT
TGTCCCCTTCAGCCACGAGGTCTCGGTCATCGCAGCCCGCAGCCTCAATGGCAAAGTCGCCTGCTATGATCCGGGCGAGA
ACGTGCATCTCGAAGGCATCCTCAGCACCACCACCGTGCCCGCAAACCTCACGACGGGTCAGAGCATGGATGCGGTGCTG
ATGGCCGGTAAGATCCTGAACGCCCTCGATTACGTTGGTGTTCTGGCGGTCGAGATCTTTGTCACCGCGCATGGCCTAGT
GGTCAATGAAATCGCCCCGCGCGTGCACAACTCGGGTCACTGGACGCAGGAAGGCTGCACCATCGACCAGTTCGAACAGC
ACATCCGGGCCATCACAGGCTGGACCATCGGCAATGGCGCCCGTTACGCCGATGTTGTGATGGAAAACCTGATTGGCGAG
GACGTGGGACGTATCGCCGATCTTGCACGCGATCCCGATTGCGCCATTCATCTCTATGGCAAGGCCGAAACAAAGCCTGG
CCGCAAGATGGGCCATGTAAACCGTGTCATCCACACCGAAGGGCGCGAGGGCTACTGA

Upstream 100 bases:

>100_bases
ATGGCCGCCGGCATTCTCGCCACCAGCGACGCCGCGCTGGCAGAACGTCTTGACGCCTGGCGCGACGCGCTTGCCGCCTC
CATTCCCGAGGAGCCTACCG

Downstream 100 bases:

>100_bases
CACGCCACCATCCTCTGGCGACTCCGCTGGTTTGTCTCCTGCCCCCGCGGCCGGAAGAGCGAACTGGAGGAGCCAGAGGA
CCGCACCACGAAGACGTCTC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTDLDQILEPGAVIGILGGGQLGRMLSVAASRLGFTTHIYEPAANPPAGHVADRVTTAAYEDEAALRAFAQTVDVITYEF
ENIPTSALDILEDLKPIRPGREALRVSQDRLTEKTFLQELGLATAPFAEVDDAASLEVALEKVGTPSILKTRRFGYDGKG
QMRIMHPEEAPEALAAMQGAPAVLEGFVPFSHEVSVIAARSLNGKVACYDPGENVHLEGILSTTTVPANLTTGQSMDAVL
MAGKILNALDYVGVLAVEIFVTAHGLVVNEIAPRVHNSGHWTQEGCTIDQFEQHIRAITGWTIGNGARYADVVMENLIGE
DVGRIADLARDPDCAIHLYGKAETKPGRKMGHVNRVIHTEGREGY

Sequences:

>Translated_365_residues
MTDLDQILEPGAVIGILGGGQLGRMLSVAASRLGFTTHIYEPAANPPAGHVADRVTTAAYEDEAALRAFAQTVDVITYEF
ENIPTSALDILEDLKPIRPGREALRVSQDRLTEKTFLQELGLATAPFAEVDDAASLEVALEKVGTPSILKTRRFGYDGKG
QMRIMHPEEAPEALAAMQGAPAVLEGFVPFSHEVSVIAARSLNGKVACYDPGENVHLEGILSTTTVPANLTTGQSMDAVL
MAGKILNALDYVGVLAVEIFVTAHGLVVNEIAPRVHNSGHWTQEGCTIDQFEQHIRAITGWTIGNGARYADVVMENLIGE
DVGRIADLARDPDCAIHLYGKAETKPGRKMGHVNRVIHTEGREGY
>Mature_364_residues
TDLDQILEPGAVIGILGGGQLGRMLSVAASRLGFTTHIYEPAANPPAGHVADRVTTAAYEDEAALRAFAQTVDVITYEFE
NIPTSALDILEDLKPIRPGREALRVSQDRLTEKTFLQELGLATAPFAEVDDAASLEVALEKVGTPSILKTRRFGYDGKGQ
MRIMHPEEAPEALAAMQGAPAVLEGFVPFSHEVSVIAARSLNGKVACYDPGENVHLEGILSTTTVPANLTTGQSMDAVLM
AGKILNALDYVGVLAVEIFVTAHGLVVNEIAPRVHNSGHWTQEGCTIDQFEQHIRAITGWTIGNGARYADVVMENLIGED
VGRIADLARDPDCAIHLYGKAETKPGRKMGHVNRVIHTEGREGY

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=341, Percent_Identity=37.8299120234604, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788155, Length=367, Percent_Identity=27.7929155313352, Blast_Score=104, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324702, Length=361, Percent_Identity=32.9639889196676, Blast_Score=196, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39226; Mature: 39095

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLDQILEPGAVIGILGGGQLGRMLSVAASRLGFTTHIYEPAANPPAGHVADRVTTAAY
CCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHH
EDEAALRAFAQTVDVITYEFENIPTSALDILEDLKPIRPGREALRVSQDRLTEKTFLQEL
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLATAPFAEVDDAASLEVALEKVGTPSILKTRRFGYDGKGQMRIMHPEEAPEALAAMQGA
CCCCCCCHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCC
PAVLEGFVPFSHEVSVIAARSLNGKVACYDPGENVHLEGILSTTTVPANLTTGQSMDAVL
HHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHH
MAGKILNALDYVGVLAVEIFVTAHGLVVNEIAPRVHNSGHWTQEGCTIDQFEQHIRAITG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
WTIGNGARYADVVMENLIGEDVGRIADLARDPDCAIHLYGKAETKPGRKMGHVNRVIHTE
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHEECC
GREGY
CCCCC
>Mature Secondary Structure 
TDLDQILEPGAVIGILGGGQLGRMLSVAASRLGFTTHIYEPAANPPAGHVADRVTTAAY
CCHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHH
EDEAALRAFAQTVDVITYEFENIPTSALDILEDLKPIRPGREALRVSQDRLTEKTFLQEL
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLATAPFAEVDDAASLEVALEKVGTPSILKTRRFGYDGKGQMRIMHPEEAPEALAAMQGA
CCCCCCCHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCC
PAVLEGFVPFSHEVSVIAARSLNGKVACYDPGENVHLEGILSTTTVPANLTTGQSMDAVL
HHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHH
MAGKILNALDYVGVLAVEIFVTAHGLVVNEIAPRVHNSGHWTQEGCTIDQFEQHIRAITG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
WTIGNGARYADVVMENLIGEDVGRIADLARDPDCAIHLYGKAETKPGRKMGHVNRVIHTE
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHEECC
GREGY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]