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The map label for this gene is mdeA [H]

Identifier: 99080426

GI number: 99080426

Start: 624873

End: 626165

Strand: Reverse

Name: mdeA [H]

Synonym: TM1040_0585

Alternate gene names: 99080426

Gene position: 626165-624873 (Counterclockwise)

Preceding gene: 99080427

Following gene: 99080424

Centisome position: 19.56

GC content: 61.41

Gene sequence:

>1293_bases
ATGTCCGACAGCCCGACCTATGGCTTTGATACGCTACAAATTCACGCTGGCGCCCGCCCTGATCCGGCAACCGGCGCGCG
GCAGACGCCGATCTATCAATCCACTGCCTATGTGTTTCGTGACGCCGACCACGCGGCGGCGCTCTTTAACCTGCAGGAAG
TAGGCTACATCTATTCACGCCTCACCAACCCGACCGTTGCGGTCCTGCAAGAGCGTCTCGCCACGCTCGAGGGTGGTGTC
GGCGCGGTCTGCTGCTCGTCGGGGCACGCCGCACAGATCATGGCCCTGTTCCCCCTGATGGGACCCGGCAAGAACGTGGT
TGCCTCCACCCGCCTTTACGGGGGCACGGTGACGCAGTTCAGCCAGACCATCAAACGCTTCGGCTGGTCCGCAAAATTTG
TTGATTTTGACGATCTCGATGCGCTGCGCGAGGCGATCGACGATGACACGCGGGCCGTCTTCTGCGAGTCCGTTGCCAAC
CCCGGTGGCTATGTCACCGACATCCGCGCGGTCGCCGATGTGGCAGATGCCGCAGGCCTGCCCCTGATCGTCGACAACAC
CTCTGCAACGCCCTACCTGTGCCAGCCGATTTCCCAGGGCGCGACCTTGGTAGTGCATTCCACAACCAAATACCTCACCG
GCAATGGTACCGTAACCGGCGGCTGCATCGTGGATTCGGGCAAGTTCGACTGGTCCGCCTCCGACAAGTTCCCCTCGCTC
TCAGCTCCGGAACCCGCCTATCACGGGCTCAAGTTCCACGAGACCTTCGGCAATCTTGCCTTCACCTTCCACGGCATTGC
CATCGGGCTGCGTGATCTGGGCATGACCATGAACCCGCAAGCCGCACATTACACCCTGATGGGCATTGAGACCCTGTCGC
TTCGGATGCAGCGCCATGTGGAAAATGCTGTGAAAGTCGCGACTTGGCTCGAAAACGACCCGCGCGTGGATTACGTGACC
TATGCGGGTCTCCCCTCCTCGCCATATGCCGAGCGCGCCCAGCGTTGCTATCCCAAGGGCACCGGCGGGCTGTTCACAGT
CGCCATCAAAGGCGGCTATGACGCTTGTGTCAAACTGGTCGACAGCCTCGAGATCTTCTCGCATGTGGCCAATCTTGGCG
ACACCCGGTCACTCATCATCCATTCCGCGTCCACGACTCACCGCCAGCTTACGCCCGAACAGCAAGAAGCAGCGGGCGCT
GGCCCCAATGTGGTGCGTGTGTCCATCGGCATCGAGGACGCAGACGATTTGATCCGCGATCTCGATCAAGCTCTGGCCAA
GGCCTGCGGCTGA

Upstream 100 bases:

>100_bases
ATTCGGAGAATCCTTCTACCGGCTTTTGCTTGCACTGACGCCGCGCCCGGTGTGTGGTGTGGCCATAACCAGACCGATTG
CGACATGGGAGAGTTTCAAT

Downstream 100 bases:

>100_bases
GCCGTCACCGCGGTCAAACACGACTTAGAAAACTGCAAAAGGCCGGGGCATTCCCCGGCCTTTTTCCATAAATCAACAGC
CCTTCAATTCGGATCAGTTC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSDSPTYGFDTLQIHAGARPDPATGARQTPIYQSTAYVFRDADHAAALFNLQEVGYIYSRLTNPTVAVLQERLATLEGGV
GAVCCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQFSQTIKRFGWSAKFVDFDDLDALREAIDDDTRAVFCESVAN
PGGYVTDIRAVADVADAAGLPLIVDNTSATPYLCQPISQGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSASDKFPSL
SAPEPAYHGLKFHETFGNLAFTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMQRHVENAVKVATWLENDPRVDYVT
YAGLPSSPYAERAQRCYPKGTGGLFTVAIKGGYDACVKLVDSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGA
GPNVVRVSIGIEDADDLIRDLDQALAKACG

Sequences:

>Translated_430_residues
MSDSPTYGFDTLQIHAGARPDPATGARQTPIYQSTAYVFRDADHAAALFNLQEVGYIYSRLTNPTVAVLQERLATLEGGV
GAVCCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQFSQTIKRFGWSAKFVDFDDLDALREAIDDDTRAVFCESVAN
PGGYVTDIRAVADVADAAGLPLIVDNTSATPYLCQPISQGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSASDKFPSL
SAPEPAYHGLKFHETFGNLAFTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMQRHVENAVKVATWLENDPRVDYVT
YAGLPSSPYAERAQRCYPKGTGGLFTVAIKGGYDACVKLVDSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGA
GPNVVRVSIGIEDADDLIRDLDQALAKACG
>Mature_429_residues
SDSPTYGFDTLQIHAGARPDPATGARQTPIYQSTAYVFRDADHAAALFNLQEVGYIYSRLTNPTVAVLQERLATLEGGVG
AVCCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQFSQTIKRFGWSAKFVDFDDLDALREAIDDDTRAVFCESVANP
GGYVTDIRAVADVADAAGLPLIVDNTSATPYLCQPISQGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSASDKFPSLS
APEPAYHGLKFHETFGNLAFTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMQRHVENAVKVATWLENDPRVDYVTY
AGLPSSPYAERAQRCYPKGTGGLFTVAIKGGYDACVKLVDSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGAG
PNVVRVSIGIEDADDLIRDLDQALAKACG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=31.2056737588652, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=29.3144208037825, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=27.4881516587678, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1790375, Length=418, Percent_Identity=31.8181818181818, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=25.8741258741259, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=417, Percent_Identity=30.4556354916067, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=433, Percent_Identity=32.1016166281755, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=44.0835266821346, Blast_Score=340, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=32.5581395348837, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6321254, Length=451, Percent_Identity=26.3858093126386, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=362, Percent_Identity=24.585635359116, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=32.9411764705882, Blast_Score=192, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45966; Mature: 45835

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSPTYGFDTLQIHAGARPDPATGARQTPIYQSTAYVFRDADHAAALFNLQEVGYIYSR
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LTNPTVAVLQERLATLEGGVGAVCCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQF
HCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEECEECCCHHHHH
SQTIKRFGWSAKFVDFDDLDALREAIDDDTRAVFCESVANPGGYVTDIRAVADVADAAGL
HHHHHHHCCCCEECCCHHHHHHHHHHCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHCCC
PLIVDNTSATPYLCQPISQGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSASDKFPSL
CEEEECCCCCCCEECCCCCCCEEEEECCEEEEECCCEEECCEEEECCCCCCCCCCCCCCC
SAPEPAYHGLKFHETFGNLAFTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMQRHV
CCCCCHHCCCCHHHHHCCCEEEEHHHEEEHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHH
ENAVKVATWLENDPRVDYVTYAGLPSSPYAERAQRCYPKGTGGLFTVAIKGGYDACVKLV
HHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHH
DSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGAGPNVVRVSIGIEDADDLIRD
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCHHHHHHH
LDQALAKACG
HHHHHHHHCC
>Mature Secondary Structure 
SDSPTYGFDTLQIHAGARPDPATGARQTPIYQSTAYVFRDADHAAALFNLQEVGYIYSR
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LTNPTVAVLQERLATLEGGVGAVCCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQF
HCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEECEECCCHHHHH
SQTIKRFGWSAKFVDFDDLDALREAIDDDTRAVFCESVANPGGYVTDIRAVADVADAAGL
HHHHHHHCCCCEECCCHHHHHHHHHHCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHCCC
PLIVDNTSATPYLCQPISQGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSASDKFPSL
CEEEECCCCCCCEECCCCCCCEEEEECCEEEEECCCEEECCEEEECCCCCCCCCCCCCCC
SAPEPAYHGLKFHETFGNLAFTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMQRHV
CCCCCHHCCCCHHHHHCCCEEEEHHHEEEHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHH
ENAVKVATWLENDPRVDYVTYAGLPSSPYAERAQRCYPKGTGGLFTVAIKGGYDACVKLV
HHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHH
DSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGAGPNVVRVSIGIEDADDLIRD
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCHHHHHHH
LDQALAKACG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]