| Definition | Ruegeria sp. TM1040, complete genome. |
|---|---|
| Accession | NC_008044 |
| Length | 3,200,938 |
Click here to switch to the map view.
The map label for this gene is cheR [C]
Identifier: 99080301
GI number: 99080301
Start: 477198
End: 479711
Strand: Direct
Name: cheR [C]
Synonym: TM1040_0460
Alternate gene names: 99080301
Gene position: 477198-479711 (Clockwise)
Preceding gene: 99080300
Following gene: 99080302
Centisome position: 14.91
GC content: 56.72
Gene sequence:
>2514_bases ATGAGCGACCGAAGTATTCCTTTTTTGGTTGGTATCGCTGCTTCCGCTGGGGGGCTTGAGGCTCTGTCCGAACTGGTTCA GGAGCTCGGAGAGCAAAATGAAGCGGCCTATGTGATTGCCCAGCATATGTCGCCAAATCACAAGAGCATGCTCAGTGCGT TGATTTCTCGCGAAACGAGATTGCCGGTTCGTGAGCTGACACCCGATGCGGACATCTGGCCGGAGGCCAACACGATTTAT GTGGTGATGCCCGGCCATGACGTCATCGTCACCGAAGGTGGCCAGCTGGGGCTGCGAGCACCGAGCGGGCTGCGCGGGCA GCCAAAACCGCTTGCGGATCTGTTGTTGAAAACCATGGCCGAGGAATGCGGCGAAAACTGCGCCGCGATTATTCTTTCAG GCACTGGGAGTGATGGCAGCTATGGGGTGCGCGCCATTCGCGAGGTGGGCGGCATTACCATTGCACAGGATCCCGAGAGT TCCAAATATTCCGGGATGCCGACCTCTGCGTTGCAAACCGGCTGCGTTGATCTGACGCTTCGTCCGCGTGAAATCGGTGA GCACTTGGGGAAAATCCTTTCCCGTCCCCGGGATTTCACCGAATTGCAGGAGCTGCATCAAAAGCCAAATCACCTGTCTG ATCTGTTCCAGATCCTGACGGCGCGCACCGGTGTGGATTTTCGCGAATACAAGGCGTCGACCATCAACCGCCGCATTGCG CGTCGGATGCTGGCCCTCGGTTTTTCGGAATACAACGCCTATGTGGATTTTTGCCGCAGCGACCTTGCTGAAGTGGATGC GCTTTATAAGGATCTGATGATCTCCGTAACGCGGTTCTTTCGCGATCCCCAGCAATTTGATGCCCTGAAGGCCGATATTC AAAAGCTGGTTGCAAAGCTCGAATCCTCATCCGATCAGCGAACGGTTCGCGTCTGGGTGTCTGGCTGCGCCACAGGCGAA GAAGCCTATTCGGTTGCAATCCTGTTTTTGGAAGCGATGGGCGGCCTTGAAAAAGTATCCCAGCGCCGATTGCAGGTGTT TGCCACCGATATTGACCAGCATGCACTGGATGTGGCGCGACGTGGGGAATATCCGATCTCTGCGATCAATGACATTCCGC AGGAATTGGTTGATCGCTATTTTGTCGAGCGCACTGACACCATCGAGGTCAAAAAGGCGCTTCGGGCTTTTGTCCTGTTT TCGCGCCACAACGTCATCCAGGATCCGCCGTTCACCAATCTCGACTGTGTGAGCCTGCGTAACCTGCTGATTTATTTCAA TGGGCCCTTGCAGGACAAAGTGCTCGCACGGATCGGTTACGCTCTGGTCCCTGATGGGCTGCTGTTCCTTGGGGCTGCCG AAACCGTGGGTGGGATGGAGACGATCTTTGAACCCACCAGCACCTCCGACCGGATCTACCGCAAAAGACTCGTGACGCGC AAACCCGCGCTGCAACCGTTCATCGAGGATGCAACGGCCCGGCGCCCCTTAGAGCAGTCCGCGCTGCCGTATCTGGCTGA CAAGAATAAGCACCATAGTGCGACACGACAATTTGACAATCTGATCCGTGTGTTGGCCCCAAATGGTTTCCTCGCCAGCG CCACAGGCGCGATCCTGCGCGTGATTGGCGACATCTCATTTGTGACCGCACTGACGGAAAACACCGGGCTGCAGGTGGAC CTCAAGATCCTGCTCCCGGAGTTGCGCAGCGAAGCCTCAAGTCTGATCAATGTCACCTTGCGCTCACGCGGTGTGCGCAG TGGCCAGTGGCATCCTTTACCACGGTCCAAGCATGAACAGGTGCGTCTGACCTGCTATCCTATCGTCTCCGCGCCAGACG GTGAAATGGACGGCAACAGCATTGTTCTGATCGGCGTCGAATCTCGGATCAAGGAAGAGCAGGCCGCACCGCCCGAGAGC CGCAATCCCGAGGAACAGGATCGCTATATTCGTCAGATCGAGGACGAGGTGATCTCAACCCGCGAGGCGCTCCAGCAGAC CATTGAAGAGTTGCAGACTGCCAACGAGGAACTGCAGTCGGTCAACGAGGAAATGCAGGCCGCCAACGAGGAATTGCAAT CCACCAATGAGGAGCTTGAAACCTCCAACGAGGAATTGCAGTCCACCAATGAAGAGCTGATCACCGTCAACGAGGAAATT CAGGTCAACTCCAGCGAGATCCAGCATCTGAGCGCGGAGCTCAGTGCAGTATTGCGGGCCAATCCTTTTGTGATGATCGT GGTGGATCAGGCTCTGCAGGTGCGTCACGCTTCGCGACAGGCAATGACGATGTTCGAATTGGACGCCCTTCCCAAGTCGG GCATCCACATCAGCCAATGCGCGCCCGTTGAAGGGATGCCGGATCTGACGTCCCGGATTTCTCATGTCTTCCGTACCAGT GCGCCCCTGGTCGAAAACGCCGAGTTTGGCGGTGCATCGCATGAAATGACATTCACGCCGTTTCGGGAGCGCGAAGGCCG GGACCTCATCGGGGTGGCCGTCACCATCGTCTAG
Upstream 100 bases:
>100_bases TTCCTTCCACGTCGTATCGAAGCGTCCGGGCAAGGCTTGAATTCGCCGCTGTATGCGGCGGGTGGCAATAGAGCAGGGTT TCGTCCCAGAGGACACATCA
Downstream 100 bases:
>100_bases GCGTGACATGGGATGGGGCCATGTGGGGTGGGCCTGATTTACAGGCGCTGTCGAAAACCCAGTGGTGTTTCCTACCCGAT TTGCGGCATAAACGTCGCGA
Product: MCP methyltransferase, CheR-type
Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]
Alternate protein names: ORF19 [H]
Number of amino acids: Translated: 837; Mature: 836
Protein sequence:
>837_residues MSDRSIPFLVGIAASAGGLEALSELVQELGEQNEAAYVIAQHMSPNHKSMLSALISRETRLPVRELTPDADIWPEANTIY VVMPGHDVIVTEGGQLGLRAPSGLRGQPKPLADLLLKTMAEECGENCAAIILSGTGSDGSYGVRAIREVGGITIAQDPES SKYSGMPTSALQTGCVDLTLRPREIGEHLGKILSRPRDFTELQELHQKPNHLSDLFQILTARTGVDFREYKASTINRRIA RRMLALGFSEYNAYVDFCRSDLAEVDALYKDLMISVTRFFRDPQQFDALKADIQKLVAKLESSSDQRTVRVWVSGCATGE EAYSVAILFLEAMGGLEKVSQRRLQVFATDIDQHALDVARRGEYPISAINDIPQELVDRYFVERTDTIEVKKALRAFVLF SRHNVIQDPPFTNLDCVSLRNLLIYFNGPLQDKVLARIGYALVPDGLLFLGAAETVGGMETIFEPTSTSDRIYRKRLVTR KPALQPFIEDATARRPLEQSALPYLADKNKHHSATRQFDNLIRVLAPNGFLASATGAILRVIGDISFVTALTENTGLQVD LKILLPELRSEASSLINVTLRSRGVRSGQWHPLPRSKHEQVRLTCYPIVSAPDGEMDGNSIVLIGVESRIKEEQAAPPES RNPEEQDRYIRQIEDEVISTREALQQTIEELQTANEELQSVNEEMQAANEELQSTNEELETSNEELQSTNEELITVNEEI QVNSSEIQHLSAELSAVLRANPFVMIVVDQALQVRHASRQAMTMFELDALPKSGIHISQCAPVEGMPDLTSRISHVFRTS APLVENAEFGGASHEMTFTPFREREGRDLIGVAVTIV
Sequences:
>Translated_837_residues MSDRSIPFLVGIAASAGGLEALSELVQELGEQNEAAYVIAQHMSPNHKSMLSALISRETRLPVRELTPDADIWPEANTIY VVMPGHDVIVTEGGQLGLRAPSGLRGQPKPLADLLLKTMAEECGENCAAIILSGTGSDGSYGVRAIREVGGITIAQDPES SKYSGMPTSALQTGCVDLTLRPREIGEHLGKILSRPRDFTELQELHQKPNHLSDLFQILTARTGVDFREYKASTINRRIA RRMLALGFSEYNAYVDFCRSDLAEVDALYKDLMISVTRFFRDPQQFDALKADIQKLVAKLESSSDQRTVRVWVSGCATGE EAYSVAILFLEAMGGLEKVSQRRLQVFATDIDQHALDVARRGEYPISAINDIPQELVDRYFVERTDTIEVKKALRAFVLF SRHNVIQDPPFTNLDCVSLRNLLIYFNGPLQDKVLARIGYALVPDGLLFLGAAETVGGMETIFEPTSTSDRIYRKRLVTR KPALQPFIEDATARRPLEQSALPYLADKNKHHSATRQFDNLIRVLAPNGFLASATGAILRVIGDISFVTALTENTGLQVD LKILLPELRSEASSLINVTLRSRGVRSGQWHPLPRSKHEQVRLTCYPIVSAPDGEMDGNSIVLIGVESRIKEEQAAPPES RNPEEQDRYIRQIEDEVISTREALQQTIEELQTANEELQSVNEEMQAANEELQSTNEELETSNEELQSTNEELITVNEEI QVNSSEIQHLSAELSAVLRANPFVMIVVDQALQVRHASRQAMTMFELDALPKSGIHISQCAPVEGMPDLTSRISHVFRTS APLVENAEFGGASHEMTFTPFREREGRDLIGVAVTIV >Mature_836_residues SDRSIPFLVGIAASAGGLEALSELVQELGEQNEAAYVIAQHMSPNHKSMLSALISRETRLPVRELTPDADIWPEANTIYV VMPGHDVIVTEGGQLGLRAPSGLRGQPKPLADLLLKTMAEECGENCAAIILSGTGSDGSYGVRAIREVGGITIAQDPESS KYSGMPTSALQTGCVDLTLRPREIGEHLGKILSRPRDFTELQELHQKPNHLSDLFQILTARTGVDFREYKASTINRRIAR RMLALGFSEYNAYVDFCRSDLAEVDALYKDLMISVTRFFRDPQQFDALKADIQKLVAKLESSSDQRTVRVWVSGCATGEE AYSVAILFLEAMGGLEKVSQRRLQVFATDIDQHALDVARRGEYPISAINDIPQELVDRYFVERTDTIEVKKALRAFVLFS RHNVIQDPPFTNLDCVSLRNLLIYFNGPLQDKVLARIGYALVPDGLLFLGAAETVGGMETIFEPTSTSDRIYRKRLVTRK PALQPFIEDATARRPLEQSALPYLADKNKHHSATRQFDNLIRVLAPNGFLASATGAILRVIGDISFVTALTENTGLQVDL KILLPELRSEASSLINVTLRSRGVRSGQWHPLPRSKHEQVRLTCYPIVSAPDGEMDGNSIVLIGVESRIKEEQAAPPESR NPEEQDRYIRQIEDEVISTREALQQTIEELQTANEELQSVNEEMQAANEELQSTNEELETSNEELQSTNEELITVNEEIQ VNSSEIQHLSAELSAVLRANPFVMIVVDQALQVRHASRQAMTMFELDALPKSGIHISQCAPVEGMPDLTSRISHVFRTSA PLVENAEFGGASHEMTFTPFREREGRDLIGVAVTIV
Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]
COG id: COG1352
COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 cheR-type methyltransferase domain [H]
Homologues:
Organism=Escherichia coli, GI1788193, Length=264, Percent_Identity=28.4090909090909, Blast_Score=114, Evalue=2e-26, Organism=Escherichia coli, GI1788192, Length=171, Percent_Identity=29.8245614035088, Blast_Score=74, Evalue=3e-14,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022642 - InterPro: IPR000780 - InterPro: IPR022641 - InterPro: IPR000014 - InterPro: IPR013656 - InterPro: IPR000673 [H]
Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]
EC number: 2.1.1.80 [C]
Molecular weight: Translated: 92793; Mature: 92662
Theoretical pI: Translated: 4.80; Mature: 4.80
Prosite motif: PS00092 N6_MTASE ; PS50123 CHER ; PS50122 CHEB
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSDRSIPFLVGIAASAGGLEALSELVQELGEQNEAAYVIAQHMSPNHKSMLSALISRETR CCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHC LPVRELTPDADIWPEANTIYVVMPGHDVIVTEGGQLGLRAPSGLRGQPKPLADLLLKTMA CCHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHH EECGENCAAIILSGTGSDGSYGVRAIREVGGITIAQDPESSKYSGMPTSALQTGCVDLTL HHHCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHCCCEEEEE RPREIGEHLGKILSRPRDFTELQELHQKPNHLSDLFQILTARTGVDFREYKASTINRRIA CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH RRMLALGFSEYNAYVDFCRSDLAEVDALYKDLMISVTRFFRDPQQFDALKADIQKLVAKL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH ESSSDQRTVRVWVSGCATGEEAYSVAILFLEAMGGLEKVSQRRLQVFATDIDQHALDVAR HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH RGEYPISAINDIPQELVDRYFVERTDTIEVKKALRAFVLFSRHNVIQDPPFTNLDCVSLR CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHC NLLIYFNGPLQDKVLARIGYALVPDGLLFLGAAETVGGMETIFEPTSTSDRIYRKRLVTR CEEEEECCCCHHHHHHHHCHHHCCCCCEEEECHHHHCCHHHHCCCCCCHHHHHHHHHHHC KPALQPFIEDATARRPLEQSALPYLADKNKHHSATRQFDNLIRVLAPNGFLASATGAILR CCCHHHHHHHHHHCCCCHHHCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH VIGDISFVTALTENTGLQVDLKILLPELRSEASSLINVTLRSRGVRSGQWHPLPRSKHEQ HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC VRLTCYPIVSAPDGEMDGNSIVLIGVESRIKEEQAAPPESRNPEEQDRYIRQIEDEVIST EEEEEEEEEECCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH REALQQTIEELQTANEELQSVNEEMQAANEELQSTNEELETSNEELQSTNEELITVNEEI HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCE QVNSSEIQHLSAELSAVLRANPFVMIVVDQALQVRHASRQAMTMFELDALPKSGIHISQC ECCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC APVEGMPDLTSRISHVFRTSAPLVENAEFGGASHEMTFTPFREREGRDLIGVAVTIV CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEEEC >Mature Secondary Structure SDRSIPFLVGIAASAGGLEALSELVQELGEQNEAAYVIAQHMSPNHKSMLSALISRETR CCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHC LPVRELTPDADIWPEANTIYVVMPGHDVIVTEGGQLGLRAPSGLRGQPKPLADLLLKTMA CCHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHH EECGENCAAIILSGTGSDGSYGVRAIREVGGITIAQDPESSKYSGMPTSALQTGCVDLTL HHHCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHCCCEEEEE RPREIGEHLGKILSRPRDFTELQELHQKPNHLSDLFQILTARTGVDFREYKASTINRRIA CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH RRMLALGFSEYNAYVDFCRSDLAEVDALYKDLMISVTRFFRDPQQFDALKADIQKLVAKL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH ESSSDQRTVRVWVSGCATGEEAYSVAILFLEAMGGLEKVSQRRLQVFATDIDQHALDVAR HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH RGEYPISAINDIPQELVDRYFVERTDTIEVKKALRAFVLFSRHNVIQDPPFTNLDCVSLR CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHC NLLIYFNGPLQDKVLARIGYALVPDGLLFLGAAETVGGMETIFEPTSTSDRIYRKRLVTR CEEEEECCCCHHHHHHHHCHHHCCCCCEEEECHHHHCCHHHHCCCCCCHHHHHHHHHHHC KPALQPFIEDATARRPLEQSALPYLADKNKHHSATRQFDNLIRVLAPNGFLASATGAILR CCCHHHHHHHHHHCCCCHHHCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH VIGDISFVTALTENTGLQVDLKILLPELRSEASSLINVTLRSRGVRSGQWHPLPRSKHEQ HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC VRLTCYPIVSAPDGEMDGNSIVLIGVESRIKEEQAAPPESRNPEEQDRYIRQIEDEVIST EEEEEEEEEECCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH REALQQTIEELQTANEELQSVNEEMQAANEELQSTNEELETSNEELQSTNEELITVNEEI HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCE QVNSSEIQHLSAELSAVLRANPFVMIVVDQALQVRHASRQAMTMFELDALPKSGIHISQC ECCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCC APVEGMPDLTSRISHVFRTSAPLVENAEFGGASHEMTFTPFREREGRDLIGVAVTIV CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Ca2+; Mg2+ [C]
Kcat value (1/min): 10 [C]
Specific activity: NA
Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]
Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]
Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade
General reaction: Methyl group transfer [C]
Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190 [H]