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The map label for this gene is kinD [H]

Identifier: 99080157

GI number: 99080157

Start: 321251

End: 322636

Strand: Direct

Name: kinD [H]

Synonym: TM1040_0316

Alternate gene names: 99080157

Gene position: 321251-322636 (Clockwise)

Preceding gene: 99080156

Following gene: 99080158

Centisome position: 10.04

GC content: 62.27

Gene sequence:

>1386_bases
ATGTTAAACACGCTTTCGGGCCGATTTCTGATCCTGACGACGGTCTTCGTGATGTTGGCCGAAGTGCTGATCTTTGTCCC
TTCGATCTCCCGGTTTCGGCAGGACTATCTGGAGGACCGACTGGAGCGGGCGCAGATCGCCTCGCTTGCGCTTCTGGCGG
ATGACATGATCGAAAGTGACCTGGAGCGGGAGCTGTTGGAAAATGCCGGTGTCTACAACGTCGTGCTGCGGCGGGACGAG
ATGCGCCAGCTGGTGCTGTCGTCGCCTATTCCCAGCCCGATCACAGAGACCTTCGATCTGCGCGACGCCAGTGCGCCGAC
CCTGATCGGCGATGCGTTGACGCGGCTCTGGTCGCCCGGCAATGAGATCATCCGGGTCATTGGCGCGCCGGTTCGCGACG
CAGGCCTCCTGATCGAAATCACCATGGAAACCGGGCCTTTGCGGGCCGCAATGATCGATTACGGGTTGCGTATCCTGCTG
CTCTCGGCGGTGATCTCCGTGCTGACGGCGGTGTTGTTGTTCATCGCGGTGCGCATGGTTCTGGTGACACCGATCAAGGG
GGTCATCGGCTATATGCAGCGCTATGCGGCCGCCCCCGAGGATGCGCGTGGGATCATTACGCCCAAGGCCGGGGTGATCG
AGCTGCGCGAGGCCGAAGAGGCGCTGCAAAAGCTTCAGACCGAGCTGACCCATGCGCTCAAACAGCGCGAGCGGTTGGCG
CAGCTTGGCGGTGCGGTGGCCAAGATCAGCCATGATCTGCGCAATATCCTCACTTCGGCGCAACTGTTCACCGACCGCAT
CGAAATGAGCGAAGACCCCCTGGTGCGACGTCTGGCGCCGAAACTGGTGAATTCCATCACCCGCGCGGTGTCGCTCTGTG
AAGGCACGCTGGCCTTTGGCCGCGCCGAAGAGCCAGCCCCGACATTTGGTCGGGTGTGCCTGAGCGAAATCATCGACGAT
GTGGCCGAGAGCGAAACCCTCGTGGCATCGGATGTGGACGTGGAGATCTGCAATACGGTGCCGCTGCCGACGATGATCCG
CTGCGACAAGGAGCAGATCTTTCGCGTGATCATGAACCTCGTGCGCAACGCCCGGCAAGCGATCATCGCAACCGGGCGCA
ATGGGCGCATCACGGTGTCGATGCGCGAAGAGGAAAACGCATGGTGGGTCGAGGTGGCCGATGATGGCCCGGGCCTGCCC
AAAAAGGCGCTCGACAATCTGTTCACCCCGTTTCAGGGCGGTGCGCGCAAAGGCGGCACCGGACTTGGCCTTGCCATTGC
AGCCGAGCTCATCAAGGGCCACGGCGGGCATCTTGCGCTTGCACGGACCGGTCCCGAGGGCACGGTCTTTGAGATCTGCC
TGCCCAAAGGCGATGGCGCGGTCTGA

Upstream 100 bases:

>100_bases
CGGGCGCGTATTTCTCCCCGAGACTCTTTGAAAATTCCGCCGTAAAGCGGGTTGTCCGGCTGGTGCAACGCTGGCTACCC
TGAGACGAGAACGGGGACTG

Downstream 100 bases:

>100_bases
TCTAGCAATCCTCCGTCCACTGCGCCCGCCTTTGCTGCGGATCAAGCGGCGTTGTCATAGATGTGTCAAAAAAGTTCTGA
TTTGGGGTTGCGCCTTCCCA

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase D [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLNTLSGRFLILTTVFVMLAEVLIFVPSISRFRQDYLEDRLERAQIASLALLADDMIESDLERELLENAGVYNVVLRRDE
MRQLVLSSPIPSPITETFDLRDASAPTLIGDALTRLWSPGNEIIRVIGAPVRDAGLLIEITMETGPLRAAMIDYGLRILL
LSAVISVLTAVLLFIAVRMVLVTPIKGVIGYMQRYAAAPEDARGIITPKAGVIELREAEEALQKLQTELTHALKQRERLA
QLGGAVAKISHDLRNILTSAQLFTDRIEMSEDPLVRRLAPKLVNSITRAVSLCEGTLAFGRAEEPAPTFGRVCLSEIIDD
VAESETLVASDVDVEICNTVPLPTMIRCDKEQIFRVIMNLVRNARQAIIATGRNGRITVSMREEENAWWVEVADDGPGLP
KKALDNLFTPFQGGARKGGTGLGLAIAAELIKGHGGHLALARTGPEGTVFEICLPKGDGAV

Sequences:

>Translated_461_residues
MLNTLSGRFLILTTVFVMLAEVLIFVPSISRFRQDYLEDRLERAQIASLALLADDMIESDLERELLENAGVYNVVLRRDE
MRQLVLSSPIPSPITETFDLRDASAPTLIGDALTRLWSPGNEIIRVIGAPVRDAGLLIEITMETGPLRAAMIDYGLRILL
LSAVISVLTAVLLFIAVRMVLVTPIKGVIGYMQRYAAAPEDARGIITPKAGVIELREAEEALQKLQTELTHALKQRERLA
QLGGAVAKISHDLRNILTSAQLFTDRIEMSEDPLVRRLAPKLVNSITRAVSLCEGTLAFGRAEEPAPTFGRVCLSEIIDD
VAESETLVASDVDVEICNTVPLPTMIRCDKEQIFRVIMNLVRNARQAIIATGRNGRITVSMREEENAWWVEVADDGPGLP
KKALDNLFTPFQGGARKGGTGLGLAIAAELIKGHGGHLALARTGPEGTVFEICLPKGDGAV
>Mature_461_residues
MLNTLSGRFLILTTVFVMLAEVLIFVPSISRFRQDYLEDRLERAQIASLALLADDMIESDLERELLENAGVYNVVLRRDE
MRQLVLSSPIPSPITETFDLRDASAPTLIGDALTRLWSPGNEIIRVIGAPVRDAGLLIEITMETGPLRAAMIDYGLRILL
LSAVISVLTAVLLFIAVRMVLVTPIKGVIGYMQRYAAAPEDARGIITPKAGVIELREAEEALQKLQTELTHALKQRERLA
QLGGAVAKISHDLRNILTSAQLFTDRIEMSEDPLVRRLAPKLVNSITRAVSLCEGTLAFGRAEEPAPTFGRVCLSEIIDD
VAESETLVASDVDVEICNTVPLPTMIRCDKEQIFRVIMNLVRNARQAIIATGRNGRITVSMREEENAWWVEVADDGPGLP
KKALDNLFTPFQGGARKGGTGLGLAIAAELIKGHGGHLALARTGPEGTVFEICLPKGDGAV

Specific function: Phosphorylates the sporulation-regulatory protein spo0F and, to a minor extent, is responsible for heterogeneous expression of spo0A during logarithmical growth. Also phosphorylates spo0A under biofilm growth conditions [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=29.2181069958848, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789808, Length=265, Percent_Identity=29.4339622641509, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=27.0386266094421, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790300, Length=249, Percent_Identity=29.718875502008, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=115, Percent_Identity=35.6521739130435, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=123, Percent_Identity=30.0813008130081, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786912, Length=219, Percent_Identity=26.027397260274, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50286; Mature: 50286

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTLSGRFLILTTVFVMLAEVLIFVPSISRFRQDYLEDRLERAQIASLALLADDMIESD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERELLENAGVYNVVLRRDEMRQLVLSSPIPSPITETFDLRDASAPTLIGDALTRLWSPG
HHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCH
NEIIRVIGAPVRDAGLLIEITMETGPLRAAMIDYGLRILLLSAVISVLTAVLLFIAVRMV
HHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTPIKGVIGYMQRYAAAPEDARGIITPKAGVIELREAEEALQKLQTELTHALKQRERLA
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGGAVAKISHDLRNILTSAQLFTDRIEMSEDPLVRRLAPKLVNSITRAVSLCEGTLAFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RAEEPAPTFGRVCLSEIIDDVAESETLVASDVDVEICNTVPLPTMIRCDKEQIFRVIMNL
CCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCHHHCCCHHHHHHHHHHH
VRNARQAIIATGRNGRITVSMREEENAWWVEVADDGPGLPKKALDNLFTPFQGGARKGGT
HHHHHHHEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCC
GLGLAIAAELIKGHGGHLALARTGPEGTVFEICLPKGDGAV
CHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MLNTLSGRFLILTTVFVMLAEVLIFVPSISRFRQDYLEDRLERAQIASLALLADDMIESD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERELLENAGVYNVVLRRDEMRQLVLSSPIPSPITETFDLRDASAPTLIGDALTRLWSPG
HHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCH
NEIIRVIGAPVRDAGLLIEITMETGPLRAAMIDYGLRILLLSAVISVLTAVLLFIAVRMV
HHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTPIKGVIGYMQRYAAAPEDARGIITPKAGVIELREAEEALQKLQTELTHALKQRERLA
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGGAVAKISHDLRNILTSAQLFTDRIEMSEDPLVRRLAPKLVNSITRAVSLCEGTLAFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RAEEPAPTFGRVCLSEIIDDVAESETLVASDVDVEICNTVPLPTMIRCDKEQIFRVIMNL
CCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCHHHCCCHHHHHHHHHHH
VRNARQAIIATGRNGRITVSMREEENAWWVEVADDGPGLPKKALDNLFTPFQGGARKGGT
HHHHHHHEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCC
GLGLAIAAELIKGHGGHLALARTGPEGTVFEICLPKGDGAV
CHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]