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The map label for this gene is merA [H]

Identifier: 99080156

GI number: 99080156

Start: 319818

End: 321233

Strand: Direct

Name: merA [H]

Synonym: TM1040_0315

Alternate gene names: 99080156

Gene position: 319818-321233 (Clockwise)

Preceding gene: 99080155

Following gene: 99080157

Centisome position: 9.99

GC content: 63.35

Gene sequence:

>1416_bases
ATGGCTTCGATCAAATGTGATCTGCTCATTATTGGTGCGGGGTCTGGCGGGCTGTCCCTGGCTGCCGGTGCCAGCCAGAT
GGGGGCGGATGTCGTGCTTCTTGAGGGCCACAAGATGGGCGGCGATTGCCTCAACTATGGCTGTGTCCCGTCAAAGGCGT
TGCTGGCCAGCGGCCATATCGCCCATACGCAGGCCCATGCGCAGGCGTTTGGCATCGCTGACCAGGCCGCGGAGGTGGAT
TATGCCGCCGCCAAAGGGCATGTGCAGCAGGTGATCGACACCATTGCGCCGGTGGACAGCCAAGAGCGGTTCGAAGGCTT
TGGCGTGCGCGTGATCCGCGAGATGGGCCGCTTTATCAGCGCCAACGAGGTTGCAGCGGGCGACACCACCATTACTGCCC
GGCGGATCGTGGTTGCCACGGGCTCCAGCCCCTTTGTACCGCCGATTCCGGGTCTTCAGGACGTGCCCTACCTGACCAAT
GAGACGCTCTGGGCGCTGAACGAGCGGCCTGAACATCTCATCGTGATTGGCGGCGGTCCGATCGGCATGGAGATGGCCCA
GGCGCATCGCCGTCTGGGCAGCAAAGTCACTGTGCTTGAGGCACAGGCGGCGCTGGGGCGCGATGACCCGGAAGCGGCCG
CCTTTGTGCTGGAAAGGCTGCGTAACGAGGGAATCGAGATCCGCGAGCACACCGCAGCGCAGGCCGTGCGGCAGGAGGCG
GGGCGGATCGTGGTGCAAACCAGCGCCGGGACCGTGTCCGGATCGCATCTTCTGGTGGCGGTTGGGCGCAAACCCAATCT
CGATGGGCTCAATCTGGAGGCCGCCGGGGTTGAGGTCGGCCGGGCAGGCATCAAGGTGGGGCCGGACCTGCGCAGCAGCA
ATCCCAAGGTCTATGCCATTGGCGATGTGGCCGGGCAGGGGCAATTCACCCATCTGGCGGGCTATCACGCCAGTGTCGTC
ATTCGCTCCTTGCTCTTTGGTTTGCCTGCAAAAACCAAGACGAACCATATCCCCCGCGCCACCTACACCGATCCCGAGAT
CGCGCAGGTGGGGCTGACCGAAGCCGAAGCGCAGGAGACATACGCCGCAAAGCTCGAGATTGCCCGGTTCGACTACAGCC
ACAATGACCGCGCCATCGCGACCCGGCAGGCGGAGGGCTTTATCAAGGTCATGGTGGTCAAGGGCCGCCCCGTGGGGGCC
ACGATCGCGGGGCCGCAGGCGGGGGAGCTGATCTCCACATGGTCGCTTGCGATTGCCAATGGGATGAAAATGAGCCAGAT
CGCCGCAATGGTTGCGCCGTATCCAACCATCTCTGAGCTTAACAAAAGGGTCGCGGGCGCGTATTTCTCCCCGAGACTCT
TTGAAAATTCCGCCGTAAAGCGGGTTGTCCGGCTGGTGCAACGCTGGCTACCCTGA

Upstream 100 bases:

>100_bases
GTGGGAGCCCCATATCATCGGTCCGATCCTCGGACTTTGCGCGCTGGCGGCGCTGCCGATCCTCATCAAAGCCCTGCGCG
GCACATCGGAGGTATAAGAC

Downstream 100 bases:

>100_bases
GACGAGAACGGGGACTGATGTTAAACACGCTTTCGGGCCGATTTCTGATCCTGACGACGGTCTTCGTGATGTTGGCCGAA
GTGCTGATCTTTGTCCCTTC

Product: pyridine nucleotide-disulphide oxidoreductase dimerisation region

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MASIKCDLLIIGAGSGGLSLAAGASQMGADVVLLEGHKMGGDCLNYGCVPSKALLASGHIAHTQAHAQAFGIADQAAEVD
YAAAKGHVQQVIDTIAPVDSQERFEGFGVRVIREMGRFISANEVAAGDTTITARRIVVATGSSPFVPPIPGLQDVPYLTN
ETLWALNERPEHLIVIGGGPIGMEMAQAHRRLGSKVTVLEAQAALGRDDPEAAAFVLERLRNEGIEIREHTAAQAVRQEA
GRIVVQTSAGTVSGSHLLVAVGRKPNLDGLNLEAAGVEVGRAGIKVGPDLRSSNPKVYAIGDVAGQGQFTHLAGYHASVV
IRSLLFGLPAKTKTNHIPRATYTDPEIAQVGLTEAEAQETYAAKLEIARFDYSHNDRAIATRQAEGFIKVMVVKGRPVGA
TIAGPQAGELISTWSLAIANGMKMSQIAAMVAPYPTISELNKRVAGAYFSPRLFENSAVKRVVRLVQRWLP

Sequences:

>Translated_471_residues
MASIKCDLLIIGAGSGGLSLAAGASQMGADVVLLEGHKMGGDCLNYGCVPSKALLASGHIAHTQAHAQAFGIADQAAEVD
YAAAKGHVQQVIDTIAPVDSQERFEGFGVRVIREMGRFISANEVAAGDTTITARRIVVATGSSPFVPPIPGLQDVPYLTN
ETLWALNERPEHLIVIGGGPIGMEMAQAHRRLGSKVTVLEAQAALGRDDPEAAAFVLERLRNEGIEIREHTAAQAVRQEA
GRIVVQTSAGTVSGSHLLVAVGRKPNLDGLNLEAAGVEVGRAGIKVGPDLRSSNPKVYAIGDVAGQGQFTHLAGYHASVV
IRSLLFGLPAKTKTNHIPRATYTDPEIAQVGLTEAEAQETYAAKLEIARFDYSHNDRAIATRQAEGFIKVMVVKGRPVGA
TIAGPQAGELISTWSLAIANGMKMSQIAAMVAPYPTISELNKRVAGAYFSPRLFENSAVKRVVRLVQRWLP
>Mature_470_residues
ASIKCDLLIIGAGSGGLSLAAGASQMGADVVLLEGHKMGGDCLNYGCVPSKALLASGHIAHTQAHAQAFGIADQAAEVDY
AAAKGHVQQVIDTIAPVDSQERFEGFGVRVIREMGRFISANEVAAGDTTITARRIVVATGSSPFVPPIPGLQDVPYLTNE
TLWALNERPEHLIVIGGGPIGMEMAQAHRRLGSKVTVLEAQAALGRDDPEAAAFVLERLRNEGIEIREHTAAQAVRQEAG
RIVVQTSAGTVSGSHLLVAVGRKPNLDGLNLEAAGVEVGRAGIKVGPDLRSSNPKVYAIGDVAGQGQFTHLAGYHASVVI
RSLLFGLPAKTKTNHIPRATYTDPEIAQVGLTEAEAQETYAAKLEIARFDYSHNDRAIATRQAEGFIKVMVVKGRPVGAT
IAGPQAGELISTWSLAIANGMKMSQIAAMVAPYPTISELNKRVAGAYFSPRLFENSAVKRVVRLVQRWLP

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=31.0492505353319, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=27.1334792122538, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=25.0549450549451, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148277065, Length=460, Percent_Identity=23.9130434782609, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI33519430, Length=460, Percent_Identity=23.9130434782609, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI33519428, Length=460, Percent_Identity=23.9130434782609, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI33519426, Length=460, Percent_Identity=23.9130434782609, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI148277071, Length=460, Percent_Identity=23.9130434782609, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=23.8512035010941, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=22.1729490022173, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=32.3788546255507, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=27.5167785234899, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789915, Length=427, Percent_Identity=27.4004683840749, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87082354, Length=470, Percent_Identity=26.5957446808511, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789065, Length=231, Percent_Identity=27.7056277056277, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=30.4439746300211, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17557007, Length=468, Percent_Identity=26.9230769230769, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=25.7322175732218, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=428, Percent_Identity=25.9345794392523, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=428, Percent_Identity=25.9345794392523, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=466, Percent_Identity=33.0472103004292, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=461, Percent_Identity=25.8134490238612, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=26.8085106382979, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=31.7124735729387, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24640549, Length=477, Percent_Identity=27.8825995807128, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=27.8825995807128, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24640551, Length=477, Percent_Identity=27.8825995807128, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=26.4270613107822, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49775; Mature: 49643

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIKCDLLIIGAGSGGLSLAAGASQMGADVVLLEGHKMGGDCLNYGCVPSKALLASGHI
CCCEEEEEEEEECCCCCCEEECCCHHCCCCEEEEECCCCCCHHHCCCCCCCHHHHHCCCC
AHTQAHAQAFGIADQAAEVDYAAAKGHVQQVIDTIAPVDSQERFEGFGVRVIREMGRFIS
HHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
ANEVAAGDTTITARRIVVATGSSPFVPPIPGLQDVPYLTNETLWALNERPEHLIVIGGGP
CCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCC
IGMEMAQAHRRLGSKVTVLEAQAALGRDDPEAAAFVLERLRNEGIEIREHTAAQAVRQEA
CCHHHHHHHHHCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHC
GRIVVQTSAGTVSGSHLLVAVGRKPNLDGLNLEAAGVEVGRAGIKVGPDLRSSNPKVYAI
CEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCEEECCCCCEECCCCCCCCCCEEEE
GDVAGQGQFTHLAGYHASVVIRSLLFGLPAKTKTNHIPRATYTDPEIAQVGLTEAEAQET
ECCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
YAAKLEIARFDYSHNDRAIATRQAEGFIKVMVVKGRPVGATIAGPQAGELISTWSLAIAN
HHHEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHC
GMKMSQIAAMVAPYPTISELNKRVAGAYFSPRLFENSAVKRVVRLVQRWLP
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASIKCDLLIIGAGSGGLSLAAGASQMGADVVLLEGHKMGGDCLNYGCVPSKALLASGHI
CCEEEEEEEEECCCCCCEEECCCHHCCCCEEEEECCCCCCHHHCCCCCCCHHHHHCCCC
AHTQAHAQAFGIADQAAEVDYAAAKGHVQQVIDTIAPVDSQERFEGFGVRVIREMGRFIS
HHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
ANEVAAGDTTITARRIVVATGSSPFVPPIPGLQDVPYLTNETLWALNERPEHLIVIGGGP
CCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCC
IGMEMAQAHRRLGSKVTVLEAQAALGRDDPEAAAFVLERLRNEGIEIREHTAAQAVRQEA
CCHHHHHHHHHCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHC
GRIVVQTSAGTVSGSHLLVAVGRKPNLDGLNLEAAGVEVGRAGIKVGPDLRSSNPKVYAI
CEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCEEECCCCCEECCCCCCCCCCEEEE
GDVAGQGQFTHLAGYHASVVIRSLLFGLPAKTKTNHIPRATYTDPEIAQVGLTEAEAQET
ECCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
YAAKLEIARFDYSHNDRAIATRQAEGFIKVMVVKGRPVGATIAGPQAGELISTWSLAIAN
HHHEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHC
GMKMSQIAAMVAPYPTISELNKRVAGAYFSPRLFENSAVKRVVRLVQRWLP
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]