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The map label for this gene is aceA [H]

Identifier: 94985388

GI number: 94985388

Start: 1366534

End: 1367865

Strand: Reverse

Name: aceA [H]

Synonym: Dgeo_1287

Alternate gene names: 94985388

Gene position: 1367865-1366534 (Counterclockwise)

Preceding gene: 94985389

Following gene: 94985385

Centisome position: 55.44

GC content: 64.26

Gene sequence:

>1332_bases
ATGACCCCCGCCGATCCTCGCCTGCATAGTCCCCGCACCCACGCCGAAATTCTGGAAAAGACCTGGAAGACCGAGGAACG
CTGGCAGGGGATCCGCCGCAACTACTCCGCCGACGAGGTGGTGCGGTTGCGCGGCAGCCTGCCCATCGAGCACACCCTGG
CCCGGCACGGGGCGCAAAAGCTCTGGCGACTGATGAAGGAAGAACCCTTCGTGAATGCGCTGGGGGCCCTCACTGGCAAT
CAGGCTGTGCAACAGGTGAAAGCGGGCCTGAAGGCGATCTACCTCAGTGGCTGGCAGGTGGCCGCTGACGCCAACAATGC
CGGGCAGATGTACCCCGACCAGAGCCTCTACCCGGCCTCCTCGGTGCCGGACGTGGTGCGCCGCATCAACAACGCGCTGC
GCCGCGCCGATCAGATTCAGCACTCGGAAGGCAAGGATGACATCGACTACTTCGTGCCCATTGTGGCTGACGCGGAAGCG
GGCTTCGGTGGTCCCCTCAACGCCTTTGAACTCATGAAGGCGATGATTGAGGCAGGCGCAGCGGGCGTGCACTTTGAGGA
CCAGCTCGCCTCCGAGAAGAAGTGCGGGCACCTGGGCGGCAAGGTGCTCGTGCCCACCTCACAGTTCATCCGCACCCTGA
ACGCCGCGCGCCTCGCCGCCGACGTGTCGGGCGTCCCCACCGTCCTGATCGCCCGTACCGATGCCGATGCCGCCAACCTG
CTCACCAGCGACATCGACGATAACGACAAGCCCTTCACGACCGGCGAGCGCACCCCGGAAGGCTTCTTCTACGTCCGCCC
TGGGATCGAGCAGGCGATCAGCCGCGCCCTCGCCTACGCCCCCTACGCCGATGTGCTGTGGTGCGAAACGAGCGTCCCCA
ACCTCGAGGACGCCCGCCGCTTTGCCGAAGCCGTCCACGCCAAGTTTCCCGGCAAGCTGCTCGCCTACAACTGTTCTCCC
TCCTTTAACTGGAAGAAGAACCTCGACGACGAGACGATTGCCAAATTCCAGCGCGAACTGGGCGCGATGGGCTACAAGTT
CCAGTTCATCACGCTGGCGGGCTTCCATAGTCTCAACTACTCGATGTTCGAGCTGGCCTACGGCTATGCCCGAAACCAGA
TGACGGCCTTCGTGGAGCTTCAAGAAAAGGAATTTGCGGCCCAGGAGCGGGGCTTCACCGCCGTTAAGCACCAGCGCGAG
GTGGGCACTGGCTACTTCGACCTCGTCAGCACCGCAGCAGGCGGCGGCCAGAGCAGCACCACTGCACTGGCAGGCAGTAC
CGAGGCGCAGCAGTTCCACGGGGAGAAGGAGGTGGCCGCCGCCCACGACTGA

Upstream 100 bases:

>100_bases
ACAATGGCGTCACTCCTCTCCGAGGCCAGCAGCCTCATCGGACCTCTCCCCGAGCTTGCCTGCCAGGCAGGTCAGTTTCT
CACACCCCAAAGGAGCCACC

Downstream 100 bases:

>100_bases
ACCCGGACATTCACGTCATGACCGCCGGGAGGGGAGAGCCGAAGCGAGGTTCTCCCCTCCCGGCGGTCGATTGGGCAATC
ACAGAATCCGGATTCCGGAT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTPADPRLHSPRTHAEILEKTWKTEERWQGIRRNYSADEVVRLRGSLPIEHTLARHGAQKLWRLMKEEPFVNALGALTGN
QAVQQVKAGLKAIYLSGWQVAADANNAGQMYPDQSLYPASSVPDVVRRINNALRRADQIQHSEGKDDIDYFVPIVADAEA
GFGGPLNAFELMKAMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPTSQFIRTLNAARLAADVSGVPTVLIARTDADAANL
LTSDIDDNDKPFTTGERTPEGFFYVRPGIEQAISRALAYAPYADVLWCETSVPNLEDARRFAEAVHAKFPGKLLAYNCSP
SFNWKKNLDDETIAKFQRELGAMGYKFQFITLAGFHSLNYSMFELAYGYARNQMTAFVELQEKEFAAQERGFTAVKHQRE
VGTGYFDLVSTAAGGGQSSTTALAGSTEAQQFHGEKEVAAAHD

Sequences:

>Translated_443_residues
MTPADPRLHSPRTHAEILEKTWKTEERWQGIRRNYSADEVVRLRGSLPIEHTLARHGAQKLWRLMKEEPFVNALGALTGN
QAVQQVKAGLKAIYLSGWQVAADANNAGQMYPDQSLYPASSVPDVVRRINNALRRADQIQHSEGKDDIDYFVPIVADAEA
GFGGPLNAFELMKAMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPTSQFIRTLNAARLAADVSGVPTVLIARTDADAANL
LTSDIDDNDKPFTTGERTPEGFFYVRPGIEQAISRALAYAPYADVLWCETSVPNLEDARRFAEAVHAKFPGKLLAYNCSP
SFNWKKNLDDETIAKFQRELGAMGYKFQFITLAGFHSLNYSMFELAYGYARNQMTAFVELQEKEFAAQERGFTAVKHQRE
VGTGYFDLVSTAAGGGQSSTTALAGSTEAQQFHGEKEVAAAHD
>Mature_442_residues
TPADPRLHSPRTHAEILEKTWKTEERWQGIRRNYSADEVVRLRGSLPIEHTLARHGAQKLWRLMKEEPFVNALGALTGNQ
AVQQVKAGLKAIYLSGWQVAADANNAGQMYPDQSLYPASSVPDVVRRINNALRRADQIQHSEGKDDIDYFVPIVADAEAG
FGGPLNAFELMKAMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPTSQFIRTLNAARLAADVSGVPTVLIARTDADAANLL
TSDIDDNDKPFTTGERTPEGFFYVRPGIEQAISRALAYAPYADVLWCETSVPNLEDARRFAEAVHAKFPGKLLAYNCSPS
FNWKKNLDDETIAKFQRELGAMGYKFQFITLAGFHSLNYSMFELAYGYARNQMTAFVELQEKEFAAQERGFTAVKHQREV
GTGYFDLVSTAAGGGQSSTTALAGSTEAQQFHGEKEVAAAHD

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=427, Percent_Identity=68.6182669789227, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1786525, Length=276, Percent_Identity=28.6231884057971, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17561814, Length=413, Percent_Identity=64.8910411622276, Blast_Score=556, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71982926, Length=413, Percent_Identity=64.8910411622276, Blast_Score=556, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6320908, Length=291, Percent_Identity=35.3951890034364, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325263, Length=247, Percent_Identity=37.246963562753, Blast_Score=153, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48707; Mature: 48576

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPADPRLHSPRTHAEILEKTWKTEERWQGIRRNYSADEVVRLRGSLPIEHTLARHGAQK
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHH
LWRLMKEEPFVNALGALTGNQAVQQVKAGLKAIYLSGWQVAADANNAGQMYPDQSLYPAS
HHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCC
SVPDVVRRINNALRRADQIQHSEGKDDIDYFVPIVADAEAGFGGPLNAFELMKAMIEAGA
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCC
AGVHFEDQLASEKKCGHLGGKVLVPTSQFIRTLNAARLAADVSGVPTVLIARTDADAANL
CCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH
LTSDIDDNDKPFTTGERTPEGFFYVRPGIEQAISRALAYAPYADVLWCETSVPNLEDARR
HHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
FAEAVHAKFPGKLLAYNCSPSFNWKKNLDDETIAKFQRELGAMGYKFQFITLAGFHSLNY
HHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHCCH
SMFELAYGYARNQMTAFVELQEKEFAAQERGFTAVKHQREVGTGYFDLVSTAAGGGQSST
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCC
TALAGSTEAQQFHGEKEVAAAHD
EEECCCCHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
TPADPRLHSPRTHAEILEKTWKTEERWQGIRRNYSADEVVRLRGSLPIEHTLARHGAQK
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHH
LWRLMKEEPFVNALGALTGNQAVQQVKAGLKAIYLSGWQVAADANNAGQMYPDQSLYPAS
HHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCC
SVPDVVRRINNALRRADQIQHSEGKDDIDYFVPIVADAEAGFGGPLNAFELMKAMIEAGA
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCC
AGVHFEDQLASEKKCGHLGGKVLVPTSQFIRTLNAARLAADVSGVPTVLIARTDADAANL
CCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH
LTSDIDDNDKPFTTGERTPEGFFYVRPGIEQAISRALAYAPYADVLWCETSVPNLEDARR
HHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
FAEAVHAKFPGKLLAYNCSPSFNWKKNLDDETIAKFQRELGAMGYKFQFITLAGFHSLNY
HHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHCCH
SMFELAYGYARNQMTAFVELQEKEFAAQERGFTAVKHQREVGTGYFDLVSTAAGGGQSST
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCC
TALAGSTEAQQFHGEKEVAAAHD
EEECCCCHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]