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The map label for this gene is rlmN

Identifier: 94984740

GI number: 94984740

Start: 683400

End: 684434

Strand: Reverse

Name: rlmN

Synonym: Dgeo_0633

Alternate gene names: 94984740

Gene position: 684434-683400 (Counterclockwise)

Preceding gene: 94984742

Following gene: 94984739

Centisome position: 27.74

GC content: 65.7

Gene sequence:

>1035_bases
ATGCAGCTTCTCCTCGACCTTCACCCTGATCAGTACCCGCTGGAGGGCTTCCGGCAGCGGCAACTGCTGGAGTGGGTCTT
CGTGCAGGGCGTGGGCACCTTTGAAGCCATGACCAACCTGCCCGCGCAGGCCCGCGCTGACCTCGCCTCACGTTTCCGGC
TGAATCCCTTTCGTGAGATCGAGACGGTGCGCAGCGCGGACGGCTCAGTAAAGTACCTCTTTACGCTTCAGGATGGACGG
CAGATGGAAGCCGTCTACATGCCCTATCTCGACCGCAAGACAATCTGCGTGTCCACGATGGTGGGCTGTCCCGCCAAGTG
TGCCTTTTGCGCGACGGGCGCGATGGGCTTCGGGCGGAACCTCACGCCAGGCGAGATCGTGGGGCAAGTGCTGGCGGTGG
CGGGCGGCGAAGGCCTGGCGCCGCGCGAGCTACGAAATCTGGTGTTTATGGGGATGGGCGAGCCACTGCTGAACTACGAG
AACACCATGCAGGCCGCGCGCATCCTGCTGCACCCGCAGGCGCTCGGCATGAGCAAGCGCCGCGTGACGCTCTCAACGGT
GGGCCTGCCCAAGGGGATCCGGCGGCTGGCGGCTGAGGACGACCTGGGCATCAAGCTCGCGATCAGCCTGCATGCACCTG
ACGAGGCAACCCGGCAGCGCATCATCCCGACCGGGCACCGCAACTCCATCGCCGAAATTATGGCTGCAGCCCGAGAATAC
CAGGCGGTCACCGGGCGCCGAGTCACCTTCGAGTATTCGATGTTGCGGGGAATCAACGATCATCTCTGGCAGGCGGAGGA
ACTGGCCGACCTGCTGCGCGGCCTGGTCAGTCACGTCAACCTGATCCCGATGAACCCCTGGGACGGCTCGGGCTTTGAGT
CGAGCACCGAGGAGCAGATTCAGGCCTTTTACGACGTGCTGGCAGCGCGTGGCGTGGACGTGAGCGTGCGCCGCTCGCGC
GGGAAGGACGCGGGCGCGGCGTGCGGCCAGCTCGCGCTCAAGCGGCCCGCTGCAGCGGTCGGCGCTTCTGCCTGA

Upstream 100 bases:

>100_bases
CGGCCATGTCCCACAGGGTACGGTGCCAGGCCCCGTCCCAACGTGCCTGAACGTGCTCTGCCCCTCTCCGCCACCCCGGC
TCACCCGCTAGACTTTTTGG

Downstream 100 bases:

>100_bases
CCTGGTCTCTTTTGTGAACCCTTCTGTGTCCCTATGCCGGGGCACTTTTTTTGCTCCCCTCAGCCATCGGTACGGCACGG
AGCCGGACAAGAGCTTCTCG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MQLLLDLHPDQYPLEGFRQRQLLEWVFVQGVGTFEAMTNLPAQARADLASRFRLNPFREIETVRSADGSVKYLFTLQDGR
QMEAVYMPYLDRKTICVSTMVGCPAKCAFCATGAMGFGRNLTPGEIVGQVLAVAGGEGLAPRELRNLVFMGMGEPLLNYE
NTMQAARILLHPQALGMSKRRVTLSTVGLPKGIRRLAAEDDLGIKLAISLHAPDEATRQRIIPTGHRNSIAEIMAAAREY
QAVTGRRVTFEYSMLRGINDHLWQAEELADLLRGLVSHVNLIPMNPWDGSGFESSTEEQIQAFYDVLAARGVDVSVRRSR
GKDAGAACGQLALKRPAAAVGASA

Sequences:

>Translated_344_residues
MQLLLDLHPDQYPLEGFRQRQLLEWVFVQGVGTFEAMTNLPAQARADLASRFRLNPFREIETVRSADGSVKYLFTLQDGR
QMEAVYMPYLDRKTICVSTMVGCPAKCAFCATGAMGFGRNLTPGEIVGQVLAVAGGEGLAPRELRNLVFMGMGEPLLNYE
NTMQAARILLHPQALGMSKRRVTLSTVGLPKGIRRLAAEDDLGIKLAISLHAPDEATRQRIIPTGHRNSIAEIMAAAREY
QAVTGRRVTFEYSMLRGINDHLWQAEELADLLRGLVSHVNLIPMNPWDGSGFESSTEEQIQAFYDVLAARGVDVSVRRSR
GKDAGAACGQLALKRPAAAVGASA
>Mature_344_residues
MQLLLDLHPDQYPLEGFRQRQLLEWVFVQGVGTFEAMTNLPAQARADLASRFRLNPFREIETVRSADGSVKYLFTLQDGR
QMEAVYMPYLDRKTICVSTMVGCPAKCAFCATGAMGFGRNLTPGEIVGQVLAVAGGEGLAPRELRNLVFMGMGEPLLNYE
NTMQAARILLHPQALGMSKRRVTLSTVGLPKGIRRLAAEDDLGIKLAISLHAPDEATRQRIIPTGHRNSIAEIMAAAREY
QAVTGRRVTFEYSMLRGINDHLWQAEELADLLRGLVSHVNLIPMNPWDGSGFESSTEEQIQAFYDVLAARGVDVSVRRSR
GKDAGAACGQLALKRPAAAVGASA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=323, Percent_Identity=40.5572755417957, Blast_Score=238, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_DEIGD (Q1J0P9)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_604104.1
- STRING:   Q1J0P9
- GeneID:   4057598
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0633
- NMPDR:   fig|68909.1.peg.936
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q1J0P9
- ProtClustDB:   PRK14468
- BioCyc:   DGEO319795:DGEO_0633-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 37686; Mature: 37686

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLLDLHPDQYPLEGFRQRQLLEWVFVQGVGTFEAMTNLPAQARADLASRFRLNPFREI
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHH
ETVRSADGSVKYLFTLQDGRQMEAVYMPYLDRKTICVSTMVGCPAKCAFCATGAMGFGRN
HHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCC
LTPGEIVGQVLAVAGGEGLAPRELRNLVFMGMGEPLLNYENTMQAARILLHPQALGMSKR
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCHHHHCCCCC
RVTLSTVGLPKGIRRLAAEDDLGIKLAISLHAPDEATRQRIIPTGHRNSIAEIMAAAREY
EEEEEECCCCHHHHHHHCCCCCCEEEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHH
QAVTGRRVTFEYSMLRGINDHLWQAEELADLLRGLVSHVNLIPMNPWDGSGFESSTEEQI
HHHCCCEEEEEHHHHHCCHHHHCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCHHHHH
QAFYDVLAARGVDVSVRRSRGKDAGAACGQLALKRPAAAVGASA
HHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MQLLLDLHPDQYPLEGFRQRQLLEWVFVQGVGTFEAMTNLPAQARADLASRFRLNPFREI
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHH
ETVRSADGSVKYLFTLQDGRQMEAVYMPYLDRKTICVSTMVGCPAKCAFCATGAMGFGRN
HHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCC
LTPGEIVGQVLAVAGGEGLAPRELRNLVFMGMGEPLLNYENTMQAARILLHPQALGMSKR
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHCHHHHCCCCC
RVTLSTVGLPKGIRRLAAEDDLGIKLAISLHAPDEATRQRIIPTGHRNSIAEIMAAAREY
EEEEEECCCCHHHHHHHCCCCCCEEEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHH
QAVTGRRVTFEYSMLRGINDHLWQAEELADLLRGLVSHVNLIPMNPWDGSGFESSTEEQI
HHHCCCEEEEEHHHHHCCHHHHCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCHHHHH
QAFYDVLAARGVDVSVRRSRGKDAGAACGQLALKRPAAAVGASA
HHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA