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The map label for this gene is ispD

Identifier: 94984289

GI number: 94984289

Start: 170635

End: 171318

Strand: Reverse

Name: ispD

Synonym: Dgeo_0181

Alternate gene names: 94984289

Gene position: 171318-170635 (Counterclockwise)

Preceding gene: 94984290

Following gene: 94984288

Centisome position: 6.94

GC content: 74.12

Gene sequence:

>684_bases
GTGACCAGCGTCTGCTTCCTGGCCGGCCGCACCGCCGCCCTGATTCCCGCTGCCGGGTCCGGTACCCGCCTGGGCCGCGG
GCCCAAGGCCTTTGTCGAGGTGGCGGGCCAAAGCCTCCTGGCACGCAGCGTGGCTGCCCTTGCGCCCTGGGTGGACGAAG
TGCTCGTGGCGCTGCCGGAGGGCTTCCCTCTTCCGCCGGGCCTTCCCGCGCAGGCTATCCTGGGCGGCACGACCCGGCAG
GAGAGCGTGTGGCGGCTGCTGCACGCGACCACAGCGGACGTGGTGCTCGTCCACGACGCGGCGCGGCCCTTCCTGCCGGG
GGCGGTGGTCACGGCGCTCCTTGAGGCCGTGTCCGAGACCGGCGCCGCCACCGCCGCCCTGCCGGTGGCCGATACCTTGG
TGCGTGGCGAGCGGGGCAGGTGGGCGGACCTTGTCCCGCGTGAGGGCCTCTGGGCCGTGCAGACGCCGCAAGCTTTCCGC
CGTGCGTTGCTGCTGCGGGCACACGCAGCGGCGCGGGCGGAGGGCTTCGGCGCCACAGACGACGCGGGCCTGATCGCGCG
GCTGGGCCTGCCGGTGAGGCTGGTGCCTGGCGACGCCCGGCTCTTCAAGGTCACCACTCCCGGCGACCTTGCGCTTGCGC
AGGCGGTGGCGGCAGTGTGGGATGCTACGTGTGATGCCCCCTGA

Upstream 100 bases:

>100_bases
CGCCTCAAACTGTCGAAGAGCTGCTGCACTTCGTCACGGCGCGGGTGCTCGATCAGTTCGGGCTGGATGCGCCGGCGTTC
CGGCGCTGGAAGGAGGAGGC

Downstream 100 bases:

>100_bases
ATGCGGAGGTCCCCCCATGCCCCTGGATCCCCTGACCCTGCGTCCCCTCGCTCCAAGCGAAACCGCCACCTATCTCGCTC
CTGCCAAGGTCAATCTGGGT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTSVCFLAGRTAALIPAAGSGTRLGRGPKAFVEVAGQSLLARSVAALAPWVDEVLVALPEGFPLPPGLPAQAILGGTTRQ
ESVWRLLHATTADVVLVHDAARPFLPGAVVTALLEAVSETGAATAALPVADTLVRGERGRWADLVPREGLWAVQTPQAFR
RALLLRAHAAARAEGFGATDDAGLIARLGLPVRLVPGDARLFKVTTPGDLALAQAVAAVWDATCDAP

Sequences:

>Translated_227_residues
MTSVCFLAGRTAALIPAAGSGTRLGRGPKAFVEVAGQSLLARSVAALAPWVDEVLVALPEGFPLPPGLPAQAILGGTTRQ
ESVWRLLHATTADVVLVHDAARPFLPGAVVTALLEAVSETGAATAALPVADTLVRGERGRWADLVPREGLWAVQTPQAFR
RALLLRAHAAARAEGFGATDDAGLIARLGLPVRLVPGDARLFKVTTPGDLALAQAVAAVWDATCDAP
>Mature_226_residues
TSVCFLAGRTAALIPAAGSGTRLGRGPKAFVEVAGQSLLARSVAALAPWVDEVLVALPEGFPLPPGLPAQAILGGTTRQE
SVWRLLHATTADVVLVHDAARPFLPGAVVTALLEAVSETGAATAALPVADTLVRGERGRWADLVPREGLWAVQTPQAFRR
ALLLRAHAAARAEGFGATDDAGLIARLGLPVRLVPGDARLFKVTTPGDLALAQAVAAVWDATCDAP

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=229, Percent_Identity=27.5109170305677, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789104, Length=217, Percent_Identity=40.0921658986175, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_DEIGD (Q1J200)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603653.1
- ProteinModelPortal:   Q1J200
- SMR:   Q1J200
- STRING:   Q1J200
- GeneID:   4058427
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0181
- NMPDR:   fig|68909.1.peg.1126
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- PhylomeDB:   Q1J200
- ProtClustDB:   CLSK445685
- BioCyc:   DGEO319795:DGEO_0181-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23379; Mature: 23248

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVCFLAGRTAALIPAAGSGTRLGRGPKAFVEVAGQSLLARSVAALAPWVDEVLVALPE
CCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFPLPPGLPAQAILGGTTRQESVWRLLHATTADVVLVHDAARPFLPGAVVTALLEAVSET
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
GAATAALPVADTLVRGERGRWADLVPREGLWAVQTPQAFRRALLLRAHAAARAEGFGATD
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
DAGLIARLGLPVRLVPGDARLFKVTTPGDLALAQAVAAVWDATCDAP
CCCHHHHCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSVCFLAGRTAALIPAAGSGTRLGRGPKAFVEVAGQSLLARSVAALAPWVDEVLVALPE
CCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFPLPPGLPAQAILGGTTRQESVWRLLHATTADVVLVHDAARPFLPGAVVTALLEAVSET
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
GAATAALPVADTLVRGERGRWADLVPREGLWAVQTPQAFRRALLLRAHAAARAEGFGATD
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
DAGLIARLGLPVRLVPGDARLFKVTTPGDLALAQAVAAVWDATCDAP
CCCHHHHCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA