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The map label for this gene is ispE

Identifier: 94984288

GI number: 94984288

Start: 169746

End: 170645

Strand: Reverse

Name: ispE

Synonym: Dgeo_0180

Alternate gene names: 94984288

Gene position: 170645-169746 (Counterclockwise)

Preceding gene: 94984289

Following gene: 94984284

Centisome position: 6.92

GC content: 70.0

Gene sequence:

>900_bases
ATGCCCCCTGAATGCGGAGGTCCCCCCATGCCCCTGGATCCCCTGACCCTGCGTCCCCTCGCTCCAAGCGAAACCGCCAC
CTATCTCGCTCCTGCCAAGGTCAATCTGGGTCTTAGCGTACGCGGCCTGCGCACAGACGGCTACCACGAACTGCACTCCG
TCATGGTGCCGCTGGTTGTGGGAGACGAACTCGAGATCGCAGCGGCCGACACGCTGACCCTGCGGGTGGAGGGGGCAGCA
TTGCCCACCGATGAGCGCAACCTGGTCTACCGGGCCGCGCGGGCGTATCTGGACGCGGCCGGGGTCAGCGGCGGCGCCAC
GATCACCCTGCGCAAGCGGCTGCCCCTGGCTTCTGGCCTGGGCGGCGGCAGCAGCGATGCGGCCACCACATTGATGGCAC
TGGCGCGGCTGTTCCCTGCTCCTGTGAACCTTCCGGCGCTGGCCCTCACGCTGGGCGCGGACGTGCCGTTTTTCCTGCTT
GGCCAGGCGGCGCTGGCGCAGGGGATCGGCGACGTGCTCACGCCGCTGCCGGTACCGCAAGTGCCGCTGGTGCTGGTCAA
TCCGGGGGTGGAGGTCAGCGCCCGCGACGCCTATGCCTGGCTGGATGAGGAGGAAGCCTTCACGCCGCCGCTCGATGTGG
AGGGCCTGCTGGCCGCCCTCACCGCCCAGCACGAGCTGCCCACCTTCAATGCTCTCCAGGGCCCGGTTGCCGCGCGCCAC
GCGCCCATCCAAGCTGCCCTGGCCGCCCTCTCGAGCGCAGGCCTGCGTTCGCCGCTGATGAGCGGCTCAGGAGCGACCTG
TTTTGCCCTGGCCGCGAGTGACGCTCAGGCACACGCTGCTGCGCAGGCGCTGCAGGCACAGCACCCGGCGTGGTGGGTGG
TGGCGACGAGGACGCTGTAG

Upstream 100 bases:

>100_bases
GTGAGGCTGGTGCCTGGCGACGCCCGGCTCTTCAAGGTCACCACTCCCGGCGACCTTGCGCTTGCGCAGGCGGTGGCGGC
AGTGTGGGATGCTACGTGTG

Downstream 100 bases:

>100_bases
GGCGGCGCGCCTCATCCGCGTGCCTGAGGGGGAGAGAGCCTGAGGTGGGGCCGTTTGACTGGCGCCTCTCCTACGAAGGG
CGCCCTTCTACGGCTGATAC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPPECGGPPMPLDPLTLRPLAPSETATYLAPAKVNLGLSVRGLRTDGYHELHSVMVPLVVGDELEIAAADTLTLRVEGAA
LPTDERNLVYRAARAYLDAAGVSGGATITLRKRLPLASGLGGGSSDAATTLMALARLFPAPVNLPALALTLGADVPFFLL
GQAALAQGIGDVLTPLPVPQVPLVLVNPGVEVSARDAYAWLDEEEAFTPPLDVEGLLAALTAQHELPTFNALQGPVAARH
APIQAALAALSSAGLRSPLMSGSGATCFALAASDAQAHAAAQALQAQHPAWWVVATRTL

Sequences:

>Translated_299_residues
MPPECGGPPMPLDPLTLRPLAPSETATYLAPAKVNLGLSVRGLRTDGYHELHSVMVPLVVGDELEIAAADTLTLRVEGAA
LPTDERNLVYRAARAYLDAAGVSGGATITLRKRLPLASGLGGGSSDAATTLMALARLFPAPVNLPALALTLGADVPFFLL
GQAALAQGIGDVLTPLPVPQVPLVLVNPGVEVSARDAYAWLDEEEAFTPPLDVEGLLAALTAQHELPTFNALQGPVAARH
APIQAALAALSSAGLRSPLMSGSGATCFALAASDAQAHAAAQALQAQHPAWWVVATRTL
>Mature_298_residues
PPECGGPPMPLDPLTLRPLAPSETATYLAPAKVNLGLSVRGLRTDGYHELHSVMVPLVVGDELEIAAADTLTLRVEGAAL
PTDERNLVYRAARAYLDAAGVSGGATITLRKRLPLASGLGGGSSDAATTLMALARLFPAPVNLPALALTLGADVPFFLLG
QAALAQGIGDVLTPLPVPQVPLVLVNPGVEVSARDAYAWLDEEEAFTPPLDVEGLLAALTAQHELPTFNALQGPVAARHA
PIQAALAALSSAGLRSPLMSGSGATCFALAASDAQAHAAAQALQAQHPAWWVVATRTL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=276, Percent_Identity=36.231884057971, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_DEIGD (Q1J201)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603652.1
- ProteinModelPortal:   Q1J201
- SMR:   Q1J201
- STRING:   Q1J201
- GeneID:   4058426
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0180
- NMPDR:   fig|68909.1.peg.1125
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- PhylomeDB:   Q1J201
- ProtClustDB:   PRK14612
- BioCyc:   DGEO319795:DGEO_0180-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30705; Mature: 30573

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: ACT_SITE 33-33 ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPECGGPPMPLDPLTLRPLAPSETATYLAPAKVNLGLSVRGLRTDGYHELHSVMVPLVV
CCCCCCCCCCCCCCCEECCCCCCCCCEEEECEEEECCEEEEEECCCCHHHHHHHHHHEEE
GDELEIAAADTLTLRVEGAALPTDERNLVYRAARAYLDAAGVSGGATITLRKRLPLASGL
CCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHCCC
GGGSSDAATTLMALARLFPAPVNLPALALTLGADVPFFLLGQAALAQGIGDVLTPLPVPQ
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
VPLVLVNPGVEVSARDAYAWLDEEEAFTPPLDVEGLLAALTAQHELPTFNALQGPVAARH
CCEEEECCCCEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHC
APIQAALAALSSAGLRSPLMSGSGATCFALAASDAQAHAAAQALQAQHPAWWVVATRTL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECC
>Mature Secondary Structure 
PPECGGPPMPLDPLTLRPLAPSETATYLAPAKVNLGLSVRGLRTDGYHELHSVMVPLVV
CCCCCCCCCCCCCCEECCCCCCCCCEEEECEEEECCEEEEEECCCCHHHHHHHHHHEEE
GDELEIAAADTLTLRVEGAALPTDERNLVYRAARAYLDAAGVSGGATITLRKRLPLASGL
CCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHCCC
GGGSSDAATTLMALARLFPAPVNLPALALTLGADVPFFLLGQAALAQGIGDVLTPLPVPQ
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
VPLVLVNPGVEVSARDAYAWLDEEEAFTPPLDVEGLLAALTAQHELPTFNALQGPVAARH
CCEEEECCCCEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHC
APIQAALAALSSAGLRSPLMSGSGATCFALAASDAQAHAAAQALQAQHPAWWVVATRTL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA