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The map label for this gene is glpK [H]

Identifier: 94971990

GI number: 94971990

Start: 524304

End: 525821

Strand: Direct

Name: glpK [H]

Synonym: Dgeo_2522

Alternate gene names: 94971990

Gene position: 524304-525821 (Clockwise)

Preceding gene: 94971991

Following gene: 94971989

Centisome position: 91.32

GC content: 66.8

Gene sequence:

>1518_bases
ATGTCCCAGTACATCCTCGCCCTCGACCAAGGCACCACCAGCAGCCGCGCCATCGTCTTTGACCGCGAAGGCAATATCCG
CTCGGTCGCGCAAAAAGAATTCGAGCAGATTTTTCCGCGTCCGGGCTGGGTCGAACATGACGCCCTAGAAATCTGGAGCA
CCCAGAGCGGCGTGGCGCAGGAGGCCATCACGCGGGCCGGACTGCGAGCGTCTGATATCGCCGCCATCGGCATCACCAAC
CAGCGCGAGACGACGGTGGTGTGGGACCGCCAGACCGGACAGCCGATTCACCACGCGATTGTCTGGCAGGACCGCCGCAC
CGCCGCCTTCTGTGATGGACTGCGCGCGCAGGGTCTGGAAGCCACCTTCCAGCAGAAGACCGGACTCATCATCGACGCCT
ATTTCAGTGGCACGAAGGTGCGCTGGATTCTAGACAACGTGGAGGGAGCGCGCGAACGAGCCGAACGGGGCGAACTGGCC
TTTGGCACGATCGATTCCTGGCTGGTGTACAAACTGACGGGCGGCGCCCTCCACATCACCGACGCAACGAATGCCAGCCG
CACCCTGCTCTACAACATCCACACCGGCGACTGGGACGACGAGCTGCTGGCTCTGCTGGATGTGCCGCGCGCCATGCTGC
CGCAGGTGCGCGACAGCAGCGAGATCTACGGTGAGACGGCGGCGGGACTCCTGGGCAGCCGCATTCCCATCGCGGGCATC
GCGGGGGATCAGCAGGCCGCGACCTTTGGGCAGGCCTGCCTGGAGCGCGGCATGGCGAAAAACACCTACGGCACCGGCTG
CTTCATGCTGATGAATACGGGCAGCGACGCGGTGCCCAGCGGCAACAAGCTGCTGTCCACCGTTGCCTGGCAGCTCCAGG
GTCAGCGGACCTACGCCCTGGAGGGCAGCATCTTTGTGGCGGGGGCAGTGGTGCAGTGGCTGCGCGATGGCCTGGGCATC
ATCCGGTCGAGCAGTGAGGTGGAGGCGCTCGCGACCAGCGTGGAGTCGTCGGAGGGAGTCTTTCTGGTGCCTGCCTTTGT
GGGCCTAGGGGCGCCCTACTGGGACAGCTACGCCCGCGGCACCCTCGTGGGCATCACCCGCGGCACCACCCGCGCGCACA
TCGCCCGCGCCGCGCTGGAGGCCATTGCCTACCAGTCAGCCGAGCTGCTGGAGGCGATGGGGCAGGACAGCGGGGCACCC
CTCAAGGAGCTGCGCGTGGACGGCGGCGCGAGCAACAACAACCTGCTGATGCAGTTTCAGGCCGACATCCTGGGCGTGCC
TGTCGTCCGGCCCAAAGTGACGGAGACGACCGCACTGGGGGCCGCCTACCTGGCGGGGCTGGCGGTCGGGTATTGGCAAG
GAACCGAGGAAATCACCCGTCTCTGGCAGGTGGACCGCGTCTTCCAGCCACAGATGGACGCCACTGAGCGCAAACGCCGC
CTGAACCGCTGGCGCCGCGCGGTCGAGCGCAGCCGCGCCTGGGAGGAAACGGAGGAGGTGGCCGCCACGCAGGCGTAG

Upstream 100 bases:

>100_bases
TTTGGGGCCGAACCATCCACCCGCCAAGCAGCCCAGGTGCAGCCAGCTAGCCGTTTGCCCGTCAGACCTTGAGACCGTTC
AACTCTTCACAGGAGCTTCT

Downstream 100 bases:

>100_bases
GTCACTGGAAAGAGAGAAGCGCGCCCGGACTGAGCTTCTCCGGGCGCGCGGTCCATCTTAAGGACTCGCTCAAGATGACT
CGCCAGTGGACATTCGTTCA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSQYILALDQGTTSSRAIVFDREGNIRSVAQKEFEQIFPRPGWVEHDALEIWSTQSGVAQEAITRAGLRASDIAAIGITN
QRETTVVWDRQTGQPIHHAIVWQDRRTAAFCDGLRAQGLEATFQQKTGLIIDAYFSGTKVRWILDNVEGARERAERGELA
FGTIDSWLVYKLTGGALHITDATNASRTLLYNIHTGDWDDELLALLDVPRAMLPQVRDSSEIYGETAAGLLGSRIPIAGI
AGDQQAATFGQACLERGMAKNTYGTGCFMLMNTGSDAVPSGNKLLSTVAWQLQGQRTYALEGSIFVAGAVVQWLRDGLGI
IRSSSEVEALATSVESSEGVFLVPAFVGLGAPYWDSYARGTLVGITRGTTRAHIARAALEAIAYQSAELLEAMGQDSGAP
LKELRVDGGASNNNLLMQFQADILGVPVVRPKVTETTALGAAYLAGLAVGYWQGTEEITRLWQVDRVFQPQMDATERKRR
LNRWRRAVERSRAWEETEEVAATQA

Sequences:

>Translated_505_residues
MSQYILALDQGTTSSRAIVFDREGNIRSVAQKEFEQIFPRPGWVEHDALEIWSTQSGVAQEAITRAGLRASDIAAIGITN
QRETTVVWDRQTGQPIHHAIVWQDRRTAAFCDGLRAQGLEATFQQKTGLIIDAYFSGTKVRWILDNVEGARERAERGELA
FGTIDSWLVYKLTGGALHITDATNASRTLLYNIHTGDWDDELLALLDVPRAMLPQVRDSSEIYGETAAGLLGSRIPIAGI
AGDQQAATFGQACLERGMAKNTYGTGCFMLMNTGSDAVPSGNKLLSTVAWQLQGQRTYALEGSIFVAGAVVQWLRDGLGI
IRSSSEVEALATSVESSEGVFLVPAFVGLGAPYWDSYARGTLVGITRGTTRAHIARAALEAIAYQSAELLEAMGQDSGAP
LKELRVDGGASNNNLLMQFQADILGVPVVRPKVTETTALGAAYLAGLAVGYWQGTEEITRLWQVDRVFQPQMDATERKRR
LNRWRRAVERSRAWEETEEVAATQA
>Mature_504_residues
SQYILALDQGTTSSRAIVFDREGNIRSVAQKEFEQIFPRPGWVEHDALEIWSTQSGVAQEAITRAGLRASDIAAIGITNQ
RETTVVWDRQTGQPIHHAIVWQDRRTAAFCDGLRAQGLEATFQQKTGLIIDAYFSGTKVRWILDNVEGARERAERGELAF
GTIDSWLVYKLTGGALHITDATNASRTLLYNIHTGDWDDELLALLDVPRAMLPQVRDSSEIYGETAAGLLGSRIPIAGIA
GDQQAATFGQACLERGMAKNTYGTGCFMLMNTGSDAVPSGNKLLSTVAWQLQGQRTYALEGSIFVAGAVVQWLRDGLGII
RSSSEVEALATSVESSEGVFLVPAFVGLGAPYWDSYARGTLVGITRGTTRAHIARAALEAIAYQSAELLEAMGQDSGAPL
KELRVDGGASNNNLLMQFQADILGVPVVRPKVTETTALGAAYLAGLAVGYWQGTEEITRLWQVDRVFQPQMDATERKRRL
NRWRRAVERSRAWEETEEVAATQA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=505, Percent_Identity=47.7227722772277, Blast_Score=461, Evalue=1e-130,
Organism=Homo sapiens, GI4504007, Length=505, Percent_Identity=47.7227722772277, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI42794763, Length=511, Percent_Identity=47.1624266144814, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI41393575, Length=501, Percent_Identity=47.1057884231537, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=34.0551181102362, Blast_Score=334, Evalue=1e-91,
Organism=Escherichia coli, GI1790361, Length=499, Percent_Identity=61.1222444889779, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=505, Percent_Identity=25.9405940594059, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787789, Length=501, Percent_Identity=26.5469061876248, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789168, Length=489, Percent_Identity=25.9713701431493, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789987, Length=455, Percent_Identity=23.956043956044, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789138, Length=302, Percent_Identity=23.1788079470199, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=499, Percent_Identity=43.0861723446894, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71986140, Length=524, Percent_Identity=26.1450381679389, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=304, Percent_Identity=30.5921052631579, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=548, Percent_Identity=37.2262773722628, Blast_Score=320, Evalue=4e-88,
Organism=Drosophila melanogaster, GI17864214, Length=510, Percent_Identity=44.3137254901961, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24654573, Length=450, Percent_Identity=45.1111111111111, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655555, Length=472, Percent_Identity=43.4322033898305, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655560, Length=472, Percent_Identity=43.4322033898305, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655570, Length=472, Percent_Identity=43.4322033898305, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655574, Length=472, Percent_Identity=43.4322033898305, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655565, Length=472, Percent_Identity=43.4322033898305, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI19922058, Length=534, Percent_Identity=33.8951310861423, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24652871, Length=501, Percent_Identity=34.9301397205589, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=32.1705426356589, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI21357867, Length=531, Percent_Identity=31.2617702448211, Blast_Score=251, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=33.6182336182336, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45552909, Length=304, Percent_Identity=31.5789473684211, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55140; Mature: 55009

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYILALDQGTTSSRAIVFDREGNIRSVAQKEFEQIFPRPGWVEHDALEIWSTQSGVAQ
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHH
EAITRAGLRASDIAAIGITNQRETTVVWDRQTGQPIHHAIVWQDRRTAAFCDGLRAQGLE
HHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHCCHHHCCCH
ATFQQKTGLIIDAYFSGTKVRWILDNVEGARERAERGELAFGTIDSWLVYKLTGGALHIT
HHHHHCCCEEEEEEECCCEEEEEEECCHHHHHHHHCCCEEEECCCCEEEEEEECCEEEEE
DATNASRTLLYNIHTGDWDDELLALLDVPRAMLPQVRDSSEIYGETAAGLLGSRIPIAGI
ECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
AGDQQAATFGQACLERGMAKNTYGTGCFMLMNTGSDAVPSGNKLLSTVAWQLQGQRTYAL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEE
EGSIFVAGAVVQWLRDGLGIIRSSSEVEALATSVESSEGVFLVPAFVGLGAPYWDSYARG
CCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TLVGITRGTTRAHIARAALEAIAYQSAELLEAMGQDSGAPLKELRVDGGASNNNLLMQFQ
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCEEEEEE
ADILGVPVVRPKVTETTALGAAYLAGLAVGYWQGTEEITRLWQVDRVFQPQMDATERKRR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
LNRWRRAVERSRAWEETEEVAATQA
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQYILALDQGTTSSRAIVFDREGNIRSVAQKEFEQIFPRPGWVEHDALEIWSTQSGVAQ
CCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHH
EAITRAGLRASDIAAIGITNQRETTVVWDRQTGQPIHHAIVWQDRRTAAFCDGLRAQGLE
HHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHCCHHHCCCH
ATFQQKTGLIIDAYFSGTKVRWILDNVEGARERAERGELAFGTIDSWLVYKLTGGALHIT
HHHHHCCCEEEEEEECCCEEEEEEECCHHHHHHHHCCCEEEECCCCEEEEEEECCEEEEE
DATNASRTLLYNIHTGDWDDELLALLDVPRAMLPQVRDSSEIYGETAAGLLGSRIPIAGI
ECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
AGDQQAATFGQACLERGMAKNTYGTGCFMLMNTGSDAVPSGNKLLSTVAWQLQGQRTYAL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEE
EGSIFVAGAVVQWLRDGLGIIRSSSEVEALATSVESSEGVFLVPAFVGLGAPYWDSYARG
CCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TLVGITRGTTRAHIARAALEAIAYQSAELLEAMGQDSGAPLKELRVDGGASNNNLLMQFQ
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCEEEEEE
ADILGVPVVRPKVTETTALGAAYLAGLAVGYWQGTEEITRLWQVDRVFQPQMDATERKRR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
LNRWRRAVERSRAWEETEEVAATQA
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]