Click here to switch to the map view.

The map label for this gene is glpF [H]

Identifier: 94971991

GI number: 94971991

Start: 523372

End: 524196

Strand: Direct

Name: glpF [H]

Synonym: Dgeo_2523

Alternate gene names: 94971991

Gene position: 523372-524196 (Clockwise)

Preceding gene: 94971994

Following gene: 94971990

Centisome position: 91.16

GC content: 63.64

Gene sequence:

>825_bases
ATGAAATTCACGCTGGCTCAGGAGTTTATGGCAGAACTGCTCGGCACGATGGTGCTGATTCTCTTCGGGGTGGGGGTGGT
GGCGATGGTCACCATCTTCGCCAGCACAAATCCCGTGATCCCCGGGGAGGTGGTCAAGGGCGGCTACACCAATGTCACGC
TGGGCTGGGGCTTTGCGGTCTTGATGGGCATCTTGATCGCCGGAACAATCAGCGGCGCGCACCTCAACCCCGCCGTCACC
ATCGCGCTGGCCGCCACCGGGCGTTTTCCTTGGGCAAAAGTGCTACCCTACATCGTCGCGCAGTTTATTGGGGCTTTCCT
GGGCGCAGCCATCGTCTTTGCGGTGTACCACGCCAAGTGGCTGGGCTTCGACCCGGACCTCTCGCGCACGGCCGGCGTGT
TTAGCACCTTCCCGGCGGTGCCCGGCTTCTGGCCCGGCTTTATCGATCAGGTGGTGGGCACCGCGCTGCTGATGGCCTTG
ATTCTGGCGATTGGTGACAAGCTGAATAACCCAGCAGGTGCCTCATGGGGCGCGCTCGCGGTGGCCTTTGTGGTGATGGC
GATCGGCATCAGCTTCGGCGGCATGAACGGCTACGCGATCAATCCCGCCCGCGACGCTGGCCCGCGCATCTTCGCGGTCT
TGGCCGGCTTCAAGAACACTGGAGACCTCAACGTGTGGCTGGTGCCCATCATCGGTCCCATTGTCGGTGCGCTGATCGGG
GCCTTTGTCTATGACCTCTTCATCGGCAGGCCACTGGCGCGCGCGGGCGAGGCCGCCGTGGGCCAGCAGGGTGTGGACCC
GGCCTTCAATCTGGAGCAGCGCTGA

Upstream 100 bases:

>100_bases
TGAACGAACCGACAATTTGATGCGGTGCAGGAGCTTGGAGCGCAGTTCGGCTGTCCGTCTTTCATGCCCGGCAGGAGGCT
GGGAACCGCGGGGGGAGCAG

Downstream 100 bases:

>100_bases
CGCGGCGTTTGGGGCCGAACCATCCACCCGCCAAGCAGCCCAGGTGCAGCCAGCTAGCCGTTTGCCCGTCAGACCTTGAG
ACCGTTCAACTCTTCACAGG

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKFTLAQEFMAELLGTMVLILFGVGVVAMVTIFASTNPVIPGEVVKGGYTNVTLGWGFAVLMGILIAGTISGAHLNPAVT
IALAATGRFPWAKVLPYIVAQFIGAFLGAAIVFAVYHAKWLGFDPDLSRTAGVFSTFPAVPGFWPGFIDQVVGTALLMAL
ILAIGDKLNNPAGASWGALAVAFVVMAIGISFGGMNGYAINPARDAGPRIFAVLAGFKNTGDLNVWLVPIIGPIVGALIG
AFVYDLFIGRPLARAGEAAVGQQGVDPAFNLEQR

Sequences:

>Translated_274_residues
MKFTLAQEFMAELLGTMVLILFGVGVVAMVTIFASTNPVIPGEVVKGGYTNVTLGWGFAVLMGILIAGTISGAHLNPAVT
IALAATGRFPWAKVLPYIVAQFIGAFLGAAIVFAVYHAKWLGFDPDLSRTAGVFSTFPAVPGFWPGFIDQVVGTALLMAL
ILAIGDKLNNPAGASWGALAVAFVVMAIGISFGGMNGYAINPARDAGPRIFAVLAGFKNTGDLNVWLVPIIGPIVGALIG
AFVYDLFIGRPLARAGEAAVGQQGVDPAFNLEQR
>Mature_274_residues
MKFTLAQEFMAELLGTMVLILFGVGVVAMVTIFASTNPVIPGEVVKGGYTNVTLGWGFAVLMGILIAGTISGAHLNPAVT
IALAATGRFPWAKVLPYIVAQFIGAFLGAAIVFAVYHAKWLGFDPDLSRTAGVFSTFPAVPGFWPGFIDQVVGTALLMAL
ILAIGDKLNNPAGASWGALAVAFVVMAIGISFGGMNGYAINPARDAGPRIFAVLAGFKNTGDLNVWLVPIIGPIVGALIG
AFVYDLFIGRPLARAGEAAVGQQGVDPAFNLEQR

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=264, Percent_Identity=44.6969696969697, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4502187, Length=264, Percent_Identity=38.2575757575758, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI157266307, Length=259, Percent_Identity=37.8378378378378, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI22538420, Length=259, Percent_Identity=35.5212355212355, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI310133356, Length=178, Percent_Identity=40.4494382022472, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI310114181, Length=178, Percent_Identity=40.4494382022472, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI4502179, Length=274, Percent_Identity=27.7372262773723, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI6912506, Length=258, Percent_Identity=26.7441860465116, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4755125, Length=255, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI4502181, Length=255, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790362, Length=264, Percent_Identity=40.1515151515151, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1787101, Length=207, Percent_Identity=30.9178743961353, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71992966, Length=260, Percent_Identity=34.2307692307692, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71994009, Length=267, Percent_Identity=34.0823970037453, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17531429, Length=283, Percent_Identity=36.3957597173145, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531431, Length=283, Percent_Identity=36.3957597173145, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564052, Length=282, Percent_Identity=32.9787234042553, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17533613, Length=286, Percent_Identity=33.2167832167832, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17544068, Length=266, Percent_Identity=34.2105263157895, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71992961, Length=259, Percent_Identity=30.5019305019305, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71993722, Length=250, Percent_Identity=26.4, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=38.8888888888889, Blast_Score=145, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6322985, Length=291, Percent_Identity=32.9896907216495, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI45550503, Length=254, Percent_Identity=26.7716535433071, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24762342, Length=237, Percent_Identity=25.3164556962025, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28483; Mature: 28483

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLAQEFMAELLGTMVLILFGVGVVAMVTIFASTNPVIPGEVVKGGYTNVTLGWGFAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEHHHHHHH
LMGILIAGTISGAHLNPAVTIALAATGRFPWAKVLPYIVAQFIGAFLGAAIVFAVYHAKW
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFDPDLSRTAGVFSTFPAVPGFWPGFIDQVVGTALLMALILAIGDKLNNPAGASWGALA
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
VAFVVMAIGISFGGMNGYAINPARDAGPRIFAVLAGFKNTGDLNVWLVPIIGPIVGALIG
HHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
AFVYDLFIGRPLARAGEAAVGQQGVDPAFNLEQR
HHHHHHHHCCCHHHHCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKFTLAQEFMAELLGTMVLILFGVGVVAMVTIFASTNPVIPGEVVKGGYTNVTLGWGFAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEHHHHHHH
LMGILIAGTISGAHLNPAVTIALAATGRFPWAKVLPYIVAQFIGAFLGAAIVFAVYHAKW
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFDPDLSRTAGVFSTFPAVPGFWPGFIDQVVGTALLMALILAIGDKLNNPAGASWGALA
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
VAFVVMAIGISFGGMNGYAINPARDAGPRIFAVLAGFKNTGDLNVWLVPIIGPIVGALIG
HHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
AFVYDLFIGRPLARAGEAAVGQQGVDPAFNLEQR
HHHHHHHHCCCHHHHCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]