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The map label for this gene is pcaR [H]

Identifier: 94314837

GI number: 94314837

Start: 119260

End: 120156

Strand: Reverse

Name: pcaR [H]

Synonym: Rmet_5918

Alternate gene names: 94314837

Gene position: 120156-119260 (Counterclockwise)

Preceding gene: 94314838

Following gene: 94314834

Centisome position: 4.66

GC content: 65.66

Gene sequence:

>897_bases
ATGCGCGATTATCGAACGCTCCCCCGCACCGTTTTATCCGCCATGGACGCTCCGAGCCCCAGCCCCATCAGTCTCAAACC
GGCGCAAGCCGACACCGTGCGCGCTGCCAAGCCGCCCGCCGACATCCTCGGCAGCTTTGCCGGCGATCCCAACTTCATGC
TCTCGCTAGCGCGCGGATTGACCGTGCTCGAGGCATTCTCCGAACGCAAGCGCCCACTGACTATTTCTCAGGTGGCACAG
CGCACGCAGTTGTCGCGCGCTTCGGTCCGCCGCTGCCTCTACACGCTGGAACAGCTTGGCTACGTCAGCCAGCAGGACGG
CCACTTCGCGCTGCGTCCGCGCGTGCTGCACCTTGGCCACGCATTTTTCTCGTCGAGTGCGATCGTCTCTCTGGCGCAGC
CCGTTCTGGATAGCCTCAGCGCGCGCGTGCACGAGACCTCGGCGCTCGCCATCCTCGACGGCACCGACATCCTGTATCTG
GTTCGCTCGGAAGTGCAGCGCGTGCTGACGCACTCGCTCGGCATGGGCAGCCGCCTGCCGGCCTATTGCACGTCCACCGG
CCGCCTGTTGCTGGCCCATCAGCCGCAACCGATCCTGGAGGCGTTCTTCGACCATGCTGAACTGCGCCCGCGCACGCTGC
TGACCAAGATCTCGCGTCCTGAGCTCGAGGCAGCCTTCCAGCGCGCCCGCGACATGGACTATGTGCTGATCGACGAGGAA
CTGGAGCCGGGATTGCGCGCAATCGCCGTGCCAGTGCGGTCGCAGGCCGGCGTGGTGCTGGCGGCCATCAGCGTCAGCGT
GAAGGCCAACAAGGTGTCGGAAGGGGAAATGATTGCGCGGCTGCTCGGCCCCATGCGGGAGGCGGCAGCCGCGATCGGCA
AGATGATTGGCAACTAA

Upstream 100 bases:

>100_bases
CAGGACCCCCGTCTTGACCGACACCCCCTTCCCCGTCGCCGTCCCGACCGGCGACATTTTTTTGTCCAGAAGGCGGCAAT
CAGCGCTCTGGGGCGTTAAG

Downstream 100 bases:

>100_bases
GCGGAAACTGAGCGGAAGCTAAGCGGAAACTGAGCCGCTTTCCGCTTGCGCCGGGGTGTCAGCCCCGGTGCGCCAGTTCG
AGCTGGAACACCAGGCGCTG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MRDYRTLPRTVLSAMDAPSPSPISLKPAQADTVRAAKPPADILGSFAGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQ
RTQLSRASVRRCLYTLEQLGYVSQQDGHFALRPRVLHLGHAFFSSSAIVSLAQPVLDSLSARVHETSALAILDGTDILYL
VRSEVQRVLTHSLGMGSRLPAYCTSTGRLLLAHQPQPILEAFFDHAELRPRTLLTKISRPELEAAFQRARDMDYVLIDEE
LEPGLRAIAVPVRSQAGVVLAAISVSVKANKVSEGEMIARLLGPMREAAAAIGKMIGN

Sequences:

>Translated_298_residues
MRDYRTLPRTVLSAMDAPSPSPISLKPAQADTVRAAKPPADILGSFAGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQ
RTQLSRASVRRCLYTLEQLGYVSQQDGHFALRPRVLHLGHAFFSSSAIVSLAQPVLDSLSARVHETSALAILDGTDILYL
VRSEVQRVLTHSLGMGSRLPAYCTSTGRLLLAHQPQPILEAFFDHAELRPRTLLTKISRPELEAAFQRARDMDYVLIDEE
LEPGLRAIAVPVRSQAGVVLAAISVSVKANKVSEGEMIARLLGPMREAAAAIGKMIGN
>Mature_298_residues
MRDYRTLPRTVLSAMDAPSPSPISLKPAQADTVRAAKPPADILGSFAGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQ
RTQLSRASVRRCLYTLEQLGYVSQQDGHFALRPRVLHLGHAFFSSSAIVSLAQPVLDSLSARVHETSALAILDGTDILYL
VRSEVQRVLTHSLGMGSRLPAYCTSTGRLLLAHQPQPILEAFFDHAELRPRTLLTKISRPELEAAFQRARDMDYVLIDEE
LEPGLRAIAVPVRSQAGVVLAAISVSVKANKVSEGEMIARLLGPMREAAAAIGKMIGN

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=245, Percent_Identity=30.2040816326531, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788131, Length=249, Percent_Identity=29.718875502008, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87082359, Length=250, Percent_Identity=27.6, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789999, Length=259, Percent_Identity=27.4131274131274, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786715, Length=228, Percent_Identity=25, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32440; Mature: 32440

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDYRTLPRTVLSAMDAPSPSPISLKPAQADTVRAAKPPADILGSFAGDPNFMLSLARGL
CCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHH
TVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTLEQLGYVSQQDGHFALRPRVLHLGH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHH
AFFSSSAIVSLAQPVLDSLSARVHETSALAILDGTDILYLVRSEVQRVLTHSLGMGSRLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC
AYCTSTGRLLLAHQPQPILEAFFDHAELRPRTLLTKISRPELEAAFQRARDMDYVLIDEE
HHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEECCC
LEPGLRAIAVPVRSQAGVVLAAISVSVKANKVSEGEMIARLLGPMREAAAAIGKMIGN
CCCCHHEEEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRDYRTLPRTVLSAMDAPSPSPISLKPAQADTVRAAKPPADILGSFAGDPNFMLSLARGL
CCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHH
TVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTLEQLGYVSQQDGHFALRPRVLHLGH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHH
AFFSSSAIVSLAQPVLDSLSARVHETSALAILDGTDILYLVRSEVQRVLTHSLGMGSRLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC
AYCTSTGRLLLAHQPQPILEAFFDHAELRPRTLLTKISRPELEAAFQRARDMDYVLIDEE
HHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEECCC
LEPGLRAIAVPVRSQAGVVLAAISVSVKANKVSEGEMIARLLGPMREAAAAIGKMIGN
CCCCHHEEEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]