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The map label for this gene is trpI [H]

Identifier: 94314835

GI number: 94314835

Start: 117300

End: 118160

Strand: Direct

Name: trpI [H]

Synonym: Rmet_5916

Alternate gene names: 94314835

Gene position: 117300-118160 (Clockwise)

Preceding gene: 94314833

Following gene: 94314836

Centisome position: 4.55

GC content: 65.27

Gene sequence:

>861_bases
ATGCACTGGCTCGGCTGGATTCGCTTCTTCGAAGCCGCCGCGCGTCACCAGAACTTCGCCCGCGCGGCCGACGAGTTGCA
CCTGACCCACGGCGCGATCAGTCGGCAGATCCGCCAGCTTGAAGCCGAACTCGGCGTGAGCCTGTTCGAACGGCGTAATC
GCGCGGTCTTTCTGACCGAGGCTGGCCGCACTCTCCAGTCCGCGGCCACGCAGTCGATGGACTTGCTTGCGGCGGCGGTC
GAGCGCATCCGCAAGCCCGCTGCGAACACCGCGCTGGTGTTGTCATGCGAGCCAACCATCGCCATGCGCTGGCTGATTCC
TCGGTTGGCACGGTTTTCCGCTCGGCATCCCGATATCCCGTTGCATCTGATGGCTGCTGGCGGCGCGATCGACTTTGCGC
GTAGCGGTGTCGACGTGGCATTGCGTCGCAATGATTTCCCGTTCGATACGCGCTGGCACACGCGGGAGGTGACGCCCGAG
CGGGTGGGGCCGGTTTGCCGGCCGGATCTGGCAGATCACGGGCTCGATGCAATGCCCTGGCTGCATACGCGCACTAGACC
CGATGCCTGGCAGCGCTGGGCGCGTAGCGCGGGGTATGTACTCACTGGCAGCACGGACGTCTGGTACGAGCACTTTTATC
TAAGCCTGCAGGCCGCGTCCGCCGGGCTGGGCTGGGCGATTGCTTCGGAGCTGATGGCCTATGACGAGTTGTCCGACGGT
CGCATGGCCGCACCCCGGGGCTTCGTGGCCGATGGCTCGGCGTACCATCTGCTTTCGCCTGTGCCGTTCGAGCATGACTC
GCGCCGGCTGGCGTTGTTCGACTGGCTCCATGCCGAGGCAAACGCATCTTCACAGGCATAG

Upstream 100 bases:

>100_bases
TGGCACGACATATGCCATTTTTATTCGCAGAAACGGGTACGGCAAACAATATATACTCGCCATATTCCGTGAGAAAAACT
GACTGATTGGCATGCCATCG

Downstream 100 bases:

>100_bases
CAAGGTGGCGCCGTTATCGCCATTCGAGTGCGTCGCGAAGTGGAAGAAGCGTTACGTGCGCCACCCCTCGGAAGTCTGTC
GCTAAAGTTACGTCGTATTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MHWLGWIRFFEAAARHQNFARAADELHLTHGAISRQIRQLEAELGVSLFERRNRAVFLTEAGRTLQSAATQSMDLLAAAV
ERIRKPAANTALVLSCEPTIAMRWLIPRLARFSARHPDIPLHLMAAGGAIDFARSGVDVALRRNDFPFDTRWHTREVTPE
RVGPVCRPDLADHGLDAMPWLHTRTRPDAWQRWARSAGYVLTGSTDVWYEHFYLSLQAASAGLGWAIASELMAYDELSDG
RMAAPRGFVADGSAYHLLSPVPFEHDSRRLALFDWLHAEANASSQA

Sequences:

>Translated_286_residues
MHWLGWIRFFEAAARHQNFARAADELHLTHGAISRQIRQLEAELGVSLFERRNRAVFLTEAGRTLQSAATQSMDLLAAAV
ERIRKPAANTALVLSCEPTIAMRWLIPRLARFSARHPDIPLHLMAAGGAIDFARSGVDVALRRNDFPFDTRWHTREVTPE
RVGPVCRPDLADHGLDAMPWLHTRTRPDAWQRWARSAGYVLTGSTDVWYEHFYLSLQAASAGLGWAIASELMAYDELSDG
RMAAPRGFVADGSAYHLLSPVPFEHDSRRLALFDWLHAEANASSQA
>Mature_286_residues
MHWLGWIRFFEAAARHQNFARAADELHLTHGAISRQIRQLEAELGVSLFERRNRAVFLTEAGRTLQSAATQSMDLLAAAV
ERIRKPAANTALVLSCEPTIAMRWLIPRLARFSARHPDIPLHLMAAGGAIDFARSGVDVALRRNDFPFDTRWHTREVTPE
RVGPVCRPDLADHGLDAMPWLHTRTRPDAWQRWARSAGYVLTGSTDVWYEHFYLSLQAASAGLGWAIASELMAYDELSDG
RMAAPRGFVADGSAYHLLSPVPFEHDSRRLALFDWLHAEANASSQA

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=304, Percent_Identity=33.2236842105263, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=30.0675675675676, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1786448, Length=260, Percent_Identity=28.4615384615385, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=40.3361344537815, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=27.7591973244147, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=255, Percent_Identity=25.4901960784314, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=128, Percent_Identity=30.46875, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790208, Length=125, Percent_Identity=35.2, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32059; Mature: 32059

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHWLGWIRFFEAAARHQNFARAADELHLTHGAISRQIRQLEAELGVSLFERRNRAVFLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEC
AGRTLQSAATQSMDLLAAAVERIRKPAANTALVLSCEPTIAMRWLIPRLARFSARHPDIP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
LHLMAAGGAIDFARSGVDVALRRNDFPFDTRWHTREVTPERVGPVCRPDLADHGLDAMPW
EEEEECCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCHHHCCCCCCCC
LHTRTRPDAWQRWARSAGYVLTGSTDVWYEHFYLSLQAASAGLGWAIASELMAYDELSDG
CCCCCCCHHHHHHHHCCCEEEECCCHHEEEEHEEEEECCCCCCHHHHHHHHHHHHHCCCC
RMAAPRGFVADGSAYHLLSPVPFEHDSRRLALFDWLHAEANASSQA
CCCCCCCEEECCCCEEECCCCCCCCCCCEEEHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHWLGWIRFFEAAARHQNFARAADELHLTHGAISRQIRQLEAELGVSLFERRNRAVFLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEC
AGRTLQSAATQSMDLLAAAVERIRKPAANTALVLSCEPTIAMRWLIPRLARFSARHPDIP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
LHLMAAGGAIDFARSGVDVALRRNDFPFDTRWHTREVTPERVGPVCRPDLADHGLDAMPW
EEEEECCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCHHHCCCCCCCC
LHTRTRPDAWQRWARSAGYVLTGSTDVWYEHFYLSLQAASAGLGWAIASELMAYDELSDG
CCCCCCCHHHHHHHHCCCEEEECCCHHEEEEHEEEEECCCCCCHHHHHHHHHHHHHCCCC
RMAAPRGFVADGSAYHLLSPVPFEHDSRRLALFDWLHAEANASSQA
CCCCCCCEEECCCCEEECCCCCCCCCCCEEEHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]