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The map label for this gene is ogt3 [H]

Identifier: 94314832

GI number: 94314832

Start: 115273

End: 115821

Strand: Direct

Name: ogt3 [H]

Synonym: Rmet_5913

Alternate gene names: 94314832

Gene position: 115273-115821 (Clockwise)

Preceding gene: 94314831

Following gene: 94314833

Centisome position: 4.47

GC content: 67.4

Gene sequence:

>549_bases
ATGAGCAGCCATCACATCATCGACAGCCCGCTGGGCGACATGCTGTTGCGTGCCGATGGCGGCGCGTTGACCGGCGTGTT
CTTTGCCGGCCAGAAGTACTACCCCGGGCTGGCGCGTCCCACTCCCGTCTCGGCGATGCCCGCAGCGGATGCGCGCGTGC
TGGAGCAGGCCGCGATGGAACTCGGCGCCTACTTCGACGGGGCATTGCATGGATTCTCCGTCCCGTTCCGGCTGGTTGGC
AGCGCGTTCCAGCAGCGGATCTGGCAAGCGCTGTGCGATATCGAATTTGGTGAGACTTCGACCTACGGACAACTCGGCGC
GGGGCTCGGCTTGCCGCCAAGCCATTCGCGGGCGGTGGGCGGTGCCGTGGGGCGCAATCCCTTGTCGATCATCGTTCCGT
GTCATCGCGTGCTTGGCGCGTCCGGCGACCTGACCGGCTACGCGGGCGGCGTGGACCGCAAGCGTGCGCTGCTGACGCTG
GAGGGCGTGCTTTATCCGGGCCAGTTGCAAGCCACGGCGCTGGGTCAGGCCGCCCTTGCCCTGGACTGA

Upstream 100 bases:

>100_bases
CCGCCGGATGGGCGCCGTGGCGCGCCTATGCGGCAATCCATCTCTGGCACCGCTACGAAGCGCTGAAGCTTCAGGGTAGC
GATATCGAGGAGCAGGCGGC

Downstream 100 bases:

>100_bases
CGAAGGCGGGAGCGCGCGTGGCGCGTCTGTTCGTCGCCATCCAGCCTCCCGCCGCGCTGGTGGCCGACCTGCTGCGCATC
GTTCCCGCACAGCACGGCAT

Product: Methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MSSHHIIDSPLGDMLLRADGGALTGVFFAGQKYYPGLARPTPVSAMPAADARVLEQAAMELGAYFDGALHGFSVPFRLVG
SAFQQRIWQALCDIEFGETSTYGQLGAGLGLPPSHSRAVGGAVGRNPLSIIVPCHRVLGASGDLTGYAGGVDRKRALLTL
EGVLYPGQLQATALGQAALALD

Sequences:

>Translated_182_residues
MSSHHIIDSPLGDMLLRADGGALTGVFFAGQKYYPGLARPTPVSAMPAADARVLEQAAMELGAYFDGALHGFSVPFRLVG
SAFQQRIWQALCDIEFGETSTYGQLGAGLGLPPSHSRAVGGAVGRNPLSIIVPCHRVLGASGDLTGYAGGVDRKRALLTL
EGVLYPGQLQATALGQAALALD
>Mature_181_residues
SSHHIIDSPLGDMLLRADGGALTGVFFAGQKYYPGLARPTPVSAMPAADARVLEQAAMELGAYFDGALHGFSVPFRLVGS
AFQQRIWQALCDIEFGETSTYGQLGAGLGLPPSHSRAVGGAVGRNPLSIIVPCHRVLGASGDLTGYAGGVDRKRALLTLE
GVLYPGQLQATALGQAALALD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=164, Percent_Identity=36.5853658536585, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=49.0566037735849, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788542, Length=121, Percent_Identity=41.3223140495868, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=45.1219512195122, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=45.1219512195122, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=40, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18877; Mature: 18746

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHHIIDSPLGDMLLRADGGALTGVFFAGQKYYPGLARPTPVSAMPAADARVLEQAAME
CCCCCCCCCCHHHEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LGAYFDGALHGFSVPFRLVGSAFQQRIWQALCDIEFGETSTYGQLGAGLGLPPSHSRAVG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCC
GAVGRNPLSIIVPCHRVLGASGDLTGYAGGVDRKRALLTLEGVLYPGQLQATALGQAALA
CCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCHHEEEEEECEECCCCCCHHHHCCCCCC
LD
CC
>Mature Secondary Structure 
SSHHIIDSPLGDMLLRADGGALTGVFFAGQKYYPGLARPTPVSAMPAADARVLEQAAME
CCCCCCCCCHHHEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LGAYFDGALHGFSVPFRLVGSAFQQRIWQALCDIEFGETSTYGQLGAGLGLPPSHSRAVG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCC
GAVGRNPLSIIVPCHRVLGASGDLTGYAGGVDRKRALLTLEGVLYPGQLQATALGQAALA
CCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCHHEEEEEECEECCCCCCHHHHCCCCCC
LD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]