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The map label for this gene is ada [H]

Identifier: 94314831

GI number: 94314831

Start: 113774

End: 115276

Strand: Direct

Name: ada [H]

Synonym: Rmet_5912

Alternate gene names: 94314831

Gene position: 113774-115276 (Clockwise)

Preceding gene: 291481477

Following gene: 94314832

Centisome position: 4.41

GC content: 68.46

Gene sequence:

>1503_bases
ATGGAACTCGATCCTGACACCTGCTACAAGGCCGTGGCCTCGCATGACCGGCGCTTCGACGGACGCTTCTTCGTCGGGGT
GTCGTCGACGGGCGTGTACTGCCGGCCGATTTGCGCCGTGCGCACGCCCAAGCGCGAGAACTGCACGTTCTACGAGACCG
CCGCCGCCGCGGAGAAACACGGCTTCAGGCCTTGTCTGCGTTGTCGCCCGGAATTGGCGCCGGGGCATGGGCTGGCGGAC
ATCTCCGGCCGGCTGGCGCAGGCGGCCGCCACGTTGATCGACGAAGGCTTCATGGCCGGCGCGGGCGTGGCCCAGCTGGC
CGCGCGGATCGGTGTGACCGAGCGTCACCTGCGCCGCCTGTTCGATGCCGAGTTCGGCGTGTCGGTGCATGAGTACGCCC
AGACGCAAAAGCTACTGCTGGCCAAGCGCCTGCTGACAGACACCGCGCTGCCCGTCACCGAGGTGGCGCTGGCAGCCGGC
TTTGGCAGCGTGCGGCGGTTCAACGACGTGCTCAAGGAACGCTATGGCCTGACGCCGCTGGCGATGCGCAAGCGCGCCGC
AGATGGCATGGCGGACCGGCTCGTGTTCGAGCTCGGCTACCGGCCGCCGATGGCGTGGGGGGCGCTGCTCGGATTTCTGG
CCGTGCGTGCCGTCGACGGCATCGAACAATGTGATGGCGAGGTGTACTCGCGCACGTTGTCGATCGAAGCGGGTGGCCGG
TGTCATGCGGGCTGCGTGCGGCTCGAGCATGTGGCGCGGCGCGCGGTGCTGCGCATCACGTTGTCCGGCGCGTTGGCGCC
CGTGATTCCGCAGGCATTGGGCAAGGTCCGGCGGTTATGCGATTTGGGCTGTCGCCCCGACATCGTCGATGCCCACCTGG
GCGAACTGGCTGCGCGGACGCCTGGAATGCGCCTGCCCGGCGCGTTCGACGGATTCGAGATCGGGGTGCGCGCGATCGTC
GGTCAGGTGATTCCGGTGGTGCAGGCGCGGCGCATTCTGGCTCGGCTGGCGCAGGTGGCCGGCGAGCCGTTGCCGGAAGC
GGCCCAGGCGGGACTGACCACGCCTGCCGGCCAGGCGCCGCTGACTCACGCCTTCCCGACCGCGCAGGCTCTGGCTGCAT
TGCCCGACGAGGCATATCGGGCGGCTGGCGTGACCGCCGCGAAGACCCGGACGATTCGTGCGTTGGCCCGCAGCGTGGCT
GCCCATGAAATCGATCTCGATCCCCACGCGCCACCTGAGGTGACCGTGGCGCAATTGCGTGCCATCGATGGCATCGGAGA
CTGGACGGCGCAATACGTCGCAATGCGCGCGTTAGGCTGGCCTGACGCATTTCCGGGCACCGACTATGCGCTGCGCAAGG
TGCTGGGCGTCGATACGGTTCGCGGGATGCAGGCGAAGACCGCCGGATGGGCGCCGTGGCGCGCCTATGCGGCAATCCAT
CTCTGGCACCGCTACGAAGCGCTGAAGCTTCAGGGTAGCGATATCGAGGAGCAGGCGGCATGA

Upstream 100 bases:

>100_bases
ATTCGGGGCCAACAATCGTGACCGCCGGCGGCATCCGGGCCCGAGGCGAATGTCCGATTCCAGCGGGTTTGTCATGTCGT
AAAGGCGTAGGATGCATGGC

Downstream 100 bases:

>100_bases
GCAGCCATCACATCATCGACAGCCCGCTGGGCGACATGCTGTTGCGTGCCGATGGCGGCGCGTTGACCGGCGTGTTCTTT
GCCGGCCAGAAGTACTACCC

Product: transcriptional regulator Ada / DNA-3-methyladenine glycosylase II / DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MELDPDTCYKAVASHDRRFDGRFFVGVSSTGVYCRPICAVRTPKRENCTFYETAAAAEKHGFRPCLRCRPELAPGHGLAD
ISGRLAQAAATLIDEGFMAGAGVAQLAARIGVTERHLRRLFDAEFGVSVHEYAQTQKLLLAKRLLTDTALPVTEVALAAG
FGSVRRFNDVLKERYGLTPLAMRKRAADGMADRLVFELGYRPPMAWGALLGFLAVRAVDGIEQCDGEVYSRTLSIEAGGR
CHAGCVRLEHVARRAVLRITLSGALAPVIPQALGKVRRLCDLGCRPDIVDAHLGELAARTPGMRLPGAFDGFEIGVRAIV
GQVIPVVQARRILARLAQVAGEPLPEAAQAGLTTPAGQAPLTHAFPTAQALAALPDEAYRAAGVTAAKTRTIRALARSVA
AHEIDLDPHAPPEVTVAQLRAIDGIGDWTAQYVAMRALGWPDAFPGTDYALRKVLGVDTVRGMQAKTAGWAPWRAYAAIH
LWHRYEALKLQGSDIEEQAA

Sequences:

>Translated_500_residues
MELDPDTCYKAVASHDRRFDGRFFVGVSSTGVYCRPICAVRTPKRENCTFYETAAAAEKHGFRPCLRCRPELAPGHGLAD
ISGRLAQAAATLIDEGFMAGAGVAQLAARIGVTERHLRRLFDAEFGVSVHEYAQTQKLLLAKRLLTDTALPVTEVALAAG
FGSVRRFNDVLKERYGLTPLAMRKRAADGMADRLVFELGYRPPMAWGALLGFLAVRAVDGIEQCDGEVYSRTLSIEAGGR
CHAGCVRLEHVARRAVLRITLSGALAPVIPQALGKVRRLCDLGCRPDIVDAHLGELAARTPGMRLPGAFDGFEIGVRAIV
GQVIPVVQARRILARLAQVAGEPLPEAAQAGLTTPAGQAPLTHAFPTAQALAALPDEAYRAAGVTAAKTRTIRALARSVA
AHEIDLDPHAPPEVTVAQLRAIDGIGDWTAQYVAMRALGWPDAFPGTDYALRKVLGVDTVRGMQAKTAGWAPWRAYAAIH
LWHRYEALKLQGSDIEEQAA
>Mature_500_residues
MELDPDTCYKAVASHDRRFDGRFFVGVSSTGVYCRPICAVRTPKRENCTFYETAAAAEKHGFRPCLRCRPELAPGHGLAD
ISGRLAQAAATLIDEGFMAGAGVAQLAARIGVTERHLRRLFDAEFGVSVHEYAQTQKLLLAKRLLTDTALPVTEVALAAG
FGSVRRFNDVLKERYGLTPLAMRKRAADGMADRLVFELGYRPPMAWGALLGFLAVRAVDGIEQCDGEVYSRTLSIEAGGR
CHAGCVRLEHVARRAVLRITLSGALAPVIPQALGKVRRLCDLGCRPDIVDAHLGELAARTPGMRLPGAFDGFEIGVRAIV
GQVIPVVQARRILARLAQVAGEPLPEAAQAGLTTPAGQAPLTHAFPTAQALAALPDEAYRAAGVTAAKTRTIRALARSVA
AHEIDLDPHAPPEVTVAQLRAIDGIGDWTAQYVAMRALGWPDAFPGTDYALRKVLGVDTVRGMQAKTAGWAPWRAYAAIH
LWHRYEALKLQGSDIEEQAA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=289, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1788542, Length=185, Percent_Identity=32.972972972973, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 53812; Mature: 53812

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDPDTCYKAVASHDRRFDGRFFVGVSSTGVYCRPICAVRTPKRENCTFYETAAAAEKH
CCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCHHHCCCCCCCCCCCHHHHHHHHHC
GFRPCLRCRPELAPGHGLADISGRLAQAAATLIDEGFMAGAGVAQLAARIGVTERHLRRL
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHH
FDAEFGVSVHEYAQTQKLLLAKRLLTDTALPVTEVALAAGFGSVRRFNDVLKERYGLTPL
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
AMRKRAADGMADRLVFELGYRPPMAWGALLGFLAVRAVDGIEQCDGEVYSRTLSIEAGGR
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEECCCCC
CHAGCVRLEHVARRAVLRITLSGALAPVIPQALGKVRRLCDLGCRPDIVDAHLGELAART
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PGMRLPGAFDGFEIGVRAIVGQVIPVVQARRILARLAQVAGEPLPEAAQAGLTTPAGQAP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
LTHAFPTAQALAALPDEAYRAAGVTAAKTRTIRALARSVAAHEIDLDPHAPPEVTVAQLR
CCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AIDGIGDWTAQYVAMRALGWPDAFPGTDYALRKVLGVDTVRGMQAKTAGWAPWRAYAAIH
HHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LWHRYEALKLQGSDIEEQAA
HHHHHHHHEECCCCCHHHCC
>Mature Secondary Structure
MELDPDTCYKAVASHDRRFDGRFFVGVSSTGVYCRPICAVRTPKRENCTFYETAAAAEKH
CCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCHHHCCCCCCCCCCCHHHHHHHHHC
GFRPCLRCRPELAPGHGLADISGRLAQAAATLIDEGFMAGAGVAQLAARIGVTERHLRRL
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHH
FDAEFGVSVHEYAQTQKLLLAKRLLTDTALPVTEVALAAGFGSVRRFNDVLKERYGLTPL
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
AMRKRAADGMADRLVFELGYRPPMAWGALLGFLAVRAVDGIEQCDGEVYSRTLSIEAGGR
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEECCCCC
CHAGCVRLEHVARRAVLRITLSGALAPVIPQALGKVRRLCDLGCRPDIVDAHLGELAART
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PGMRLPGAFDGFEIGVRAIVGQVIPVVQARRILARLAQVAGEPLPEAAQAGLTTPAGQAP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
LTHAFPTAQALAALPDEAYRAAGVTAAKTRTIRALARSVAAHEIDLDPHAPPEVTVAQLR
CCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AIDGIGDWTAQYVAMRALGWPDAFPGTDYALRKVLGVDTVRGMQAKTAGWAPWRAYAAIH
HHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LWHRYEALKLQGSDIEEQAA
HHHHHHHHEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]