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The map label for this gene is prfC [H]

Identifier: 94314727

GI number: 94314727

Start: 2570644

End: 2572236

Strand: Reverse

Name: prfC [H]

Synonym: Rmet_5808

Alternate gene names: 94314727

Gene position: 2572236-2570644 (Counterclockwise)

Preceding gene: 94314728

Following gene: 94314724

Centisome position: 99.7

GC content: 64.09

Gene sequence:

>1593_bases
GTGAGTTCGCTCGTTTCCGAAATTGCGCGTCGGCGCACATTTGCCATCATCTCCCACCCGGACGCGGGTAAGACCACACT
GACCGAGAAGCTGCTCTGGTTCGGCGGCGCAATTCAGGTGGCCGGCGAGGTGCGCGCGCGCAAGGCCGATCGCCATGCCA
CCTCGGACTGGATGGAGCTGGAGAAGCAGCGCGGCATCTCGGTGACGTCGTCGGTGATGCAGTTCCCGTATCAGGCGCGT
CCGGAGTTGCCCGAGAACATCGTGAACCTGCTCGACACGCCGGGCCACGAGGACTTCTCCGAAGACACCTACCGCACGCT
GACCGCCGTGGATTCGGCCGTGATGGTGATCGACTCGGTCAACGGCGTGGAAGCGCAGACGATCAAGTTGCTCAACGTCT
GCCGTTTGCGCGCCACGCCGATCCTCACGTTCATCAACAAGCTGGACCGAGAAGGCCGCTCGCCGATCGAACTGCTCGAT
GAAATCGAGGACGTGCTCCAGATTCAGTGCGCGCCGATGACCTGGCCGATCGGCATGGGCAAGGCGTTCCGTGGCGTGTA
TCACCTGATCGACGACAAGGTGCAGCTGTTCGACCCCAAGGGCGAGAAGGGCACGGCGGCGATCATCGACGGCATCGACA
ACCCGGAACTCGACCGTCTCCTGGGCTCGCAGGCCGAGGAGCTCCGCATCGAGGTGGAACTGGTGCGCGGCGCGTCGCAC
ACGTTCGACAAGGAAGCGTTCCTGGCCGGCAAGCAGTCGCCGGTCTACTTCGGCTCGGCCATCAACAACTTCGGTGTGCA
GTCGCTGCTCGACGCGCTGTGCGAGCTGTCGCCCCCGCCGCTGGCGCGCCAGACCGAAAGCCGCACCGTGGAGCCCGAGG
AGGCCAAGTTCACCGGCTTCGTATTCAAGATCCAGGCGAACATGGACCCGCGTCACCGCGACCGCATTGCGTTCGTGCGC
GTCTGCTCGGGCCGCTTCGATCGCGGCATGAAGCTGCTGCACGTGTCGCAGGGCAAGACTGTCGCGATCAACAACGCCAT
CACCTTCATGGCGCAGGACCGTAACACGACTGAGGAAGCCTTCGCGGGCGACATCATCGGCGTGCCGAACCACGGCACGA
TCCGTCTCGGCGACGTGTTCACGGAAGGCGAGCCGCTGCGCTTCACGGGCATCCCGTCGTTCGCGCCCGAGTTCTTCCGC
CGCGCGCGACTGAACAACCCGCTCAAGGTCAAGCAGCTGCAGAAGGGTTTGCAGCAGCTTGCCGAAGAAGGCGCGACGCA
GATGTTCCGTCCGCTGGCTTCGAACGATCTGGTGTTGGGCGCGGTCGGCATGCTGCAGTTCGATGTGGTGGCGCACCGCC
TGGAGCACGAGTACGGTGTGGACGCGATCTTTGAATCGCACGAATGCGCCACGGCCCGCTGGCTGAAGGGCGATCCGGCC
GAAATCGAGAAGCTGATCGACAAGGCCGGGCACAACGTGGCGCTCGATGGTGCGGGCGACCATGTCTACCTGGCCCCAAG
CATGGTCAACCTGCGTCTGACGCAGGAGCGCTTCCCGAATATCCGGTTCCTGGAAACGCGCGAGATCGTCTGA

Upstream 100 bases:

>100_bases
ATAGCGCCCCTGTCCGCCCTCGGGCCAGCCGCGCCCCGGTGCCGCTGCGCCGTTCGGGCCTTTGCCATCACCAGATATCA
CCTGCCAGGTTCCCCCAGCC

Downstream 100 bases:

>100_bases
TCCCCAGCGTAGTTCGAGAACGGAAACGGCCCGCAGAAGCGGGCCGTTTCCGTTGGTGCGTGAGGCGTGACCTGTCAGTA
CGCCAGCGATGCCTGGATAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMELEKQRGISVTSSVMQFPYQAR
PELPENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIELLD
EIEDVLQIQCAPMTWPIGMGKAFRGVYHLIDDKVQLFDPKGEKGTAAIIDGIDNPELDRLLGSQAEELRIEVELVRGASH
TFDKEAFLAGKQSPVYFGSAINNFGVQSLLDALCELSPPPLARQTESRTVEPEEAKFTGFVFKIQANMDPRHRDRIAFVR
VCSGRFDRGMKLLHVSQGKTVAINNAITFMAQDRNTTEEAFAGDIIGVPNHGTIRLGDVFTEGEPLRFTGIPSFAPEFFR
RARLNNPLKVKQLQKGLQQLAEEGATQMFRPLASNDLVLGAVGMLQFDVVAHRLEHEYGVDAIFESHECATARWLKGDPA
EIEKLIDKAGHNVALDGAGDHVYLAPSMVNLRLTQERFPNIRFLETREIV

Sequences:

>Translated_530_residues
MSSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMELEKQRGISVTSSVMQFPYQAR
PELPENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIELLD
EIEDVLQIQCAPMTWPIGMGKAFRGVYHLIDDKVQLFDPKGEKGTAAIIDGIDNPELDRLLGSQAEELRIEVELVRGASH
TFDKEAFLAGKQSPVYFGSAINNFGVQSLLDALCELSPPPLARQTESRTVEPEEAKFTGFVFKIQANMDPRHRDRIAFVR
VCSGRFDRGMKLLHVSQGKTVAINNAITFMAQDRNTTEEAFAGDIIGVPNHGTIRLGDVFTEGEPLRFTGIPSFAPEFFR
RARLNNPLKVKQLQKGLQQLAEEGATQMFRPLASNDLVLGAVGMLQFDVVAHRLEHEYGVDAIFESHECATARWLKGDPA
EIEKLIDKAGHNVALDGAGDHVYLAPSMVNLRLTQERFPNIRFLETREIV
>Mature_529_residues
SSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMELEKQRGISVTSSVMQFPYQARP
ELPENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIELLDE
IEDVLQIQCAPMTWPIGMGKAFRGVYHLIDDKVQLFDPKGEKGTAAIIDGIDNPELDRLLGSQAEELRIEVELVRGASHT
FDKEAFLAGKQSPVYFGSAINNFGVQSLLDALCELSPPPLARQTESRTVEPEEAKFTGFVFKIQANMDPRHRDRIAFVRV
CSGRFDRGMKLLHVSQGKTVAINNAITFMAQDRNTTEEAFAGDIIGVPNHGTIRLGDVFTEGEPLRFTGIPSFAPEFFRR
ARLNNPLKVKQLQKGLQQLAEEGATQMFRPLASNDLVLGAVGMLQFDVVAHRLEHEYGVDAIFESHECATARWLKGDPAE
IEKLIDKAGHNVALDGAGDHVYLAPSMVNLRLTQERFPNIRFLETREIV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=29.0983606557377, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.078431372549, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI25306283, Length=381, Percent_Identity=27.8215223097113, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI25306287, Length=486, Percent_Identity=26.7489711934156, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI157426893, Length=170, Percent_Identity=30, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=265, Percent_Identity=26.0377358490566, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4503483, Length=167, Percent_Identity=32.3353293413174, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310132016, Length=128, Percent_Identity=35.15625, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=128, Percent_Identity=35.15625, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=128, Percent_Identity=35.15625, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=532, Percent_Identity=60.5263157894737, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=27.9918864097363, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=33.7579617834395, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI48994988, Length=215, Percent_Identity=29.7674418604651, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=498, Percent_Identity=27.3092369477912, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=480, Percent_Identity=23.75, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=141, Percent_Identity=31.2056737588652, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=481, Percent_Identity=28.2744282744283, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=432, Percent_Identity=26.1574074074074, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324707, Length=178, Percent_Identity=32.5842696629214, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=178, Percent_Identity=32.5842696629214, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=28.2608695652174, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324166, Length=149, Percent_Identity=32.8859060402685, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582462, Length=520, Percent_Identity=28.6538461538462, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI221458488, Length=512, Percent_Identity=25.1953125, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI78706572, Length=156, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=34.965034965035, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59001; Mature: 58870

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMEL
CHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHH
EKQRGISVTSSVMQFPYQARPELPENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSV
HHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECC
NGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIELLDEIEDVLQIQCAPMTWPIGMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCC
KAFRGVYHLIDDKVQLFDPKGEKGTAAIIDGIDNPELDRLLGSQAEELRIEVELVRGASH
HHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCHHHHHHHCCCHHHHEEEEEEEECCCC
TFDKEAFLAGKQSPVYFGSAINNFGVQSLLDALCELSPPPLARQTESRTVEPEEAKFTGF
CCCCHHHHCCCCCCEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
VFKIQANMDPRHRDRIAFVRVCSGRFDRGMKLLHVSQGKTVAINNAITFMAQDRNTTEEA
EEEEECCCCCCHHHHHHEEHHHCCCCCCCEEEEEECCCCEEEEECEEEEEECCCCCCHHH
FAGDIIGVPNHGTIRLGDVFTEGEPLRFTGIPSFAPEFFRRARLNNPLKVKQLQKGLQQL
HCCCEEECCCCCEEEECCEECCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
AEEGATQMFRPLASNDLVLGAVGMLQFDVVAHRLEHEYGVDAIFESHECATARWLKGDPA
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHCCCCCHH
EIEKLIDKAGHNVALDGAGDHVYLAPSMVNLRLTQERFPNIRFLETREIV
HHHHHHHHCCCEEEEECCCCEEEECCCEEEEEEEHHHCCCEEEEECCCCC
>Mature Secondary Structure 
SSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMEL
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHH
EKQRGISVTSSVMQFPYQARPELPENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSV
HHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECC
NGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIELLDEIEDVLQIQCAPMTWPIGMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCC
KAFRGVYHLIDDKVQLFDPKGEKGTAAIIDGIDNPELDRLLGSQAEELRIEVELVRGASH
HHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCHHHHHHHCCCHHHHEEEEEEEECCCC
TFDKEAFLAGKQSPVYFGSAINNFGVQSLLDALCELSPPPLARQTESRTVEPEEAKFTGF
CCCCHHHHCCCCCCEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
VFKIQANMDPRHRDRIAFVRVCSGRFDRGMKLLHVSQGKTVAINNAITFMAQDRNTTEEA
EEEEECCCCCCHHHHHHEEHHHCCCCCCCEEEEEECCCCEEEEECEEEEEECCCCCCHHH
FAGDIIGVPNHGTIRLGDVFTEGEPLRFTGIPSFAPEFFRRARLNNPLKVKQLQKGLQQL
HCCCEEECCCCCEEEECCEECCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
AEEGATQMFRPLASNDLVLGAVGMLQFDVVAHRLEHEYGVDAIFESHECATARWLKGDPA
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHCCCCCHH
EIEKLIDKAGHNVALDGAGDHVYLAPSMVNLRLTQERFPNIRFLETREIV
HHHHHHHHCCCEEEEECCCCEEEECCCEEEEEEEHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA