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The map label for this gene is mscS [H]

Identifier: 94314722

GI number: 94314722

Start: 2562451

End: 2563305

Strand: Reverse

Name: mscS [H]

Synonym: Rmet_5803

Alternate gene names: 94314722

Gene position: 2563305-2562451 (Counterclockwise)

Preceding gene: 94314723

Following gene: 94314720

Centisome position: 99.35

GC content: 66.08

Gene sequence:

>855_bases
ATGGACCTGAAATTCATTGATGCCGCCAAAGTCGAGGCCACCTGGACCTATCTCGCGCAATTCGCGGTTAGCCAGGGGCT
GAACCTGCTGGCTGCGCTGGCGATACTGGTTGGCGGGTGGTGGTTGTCCGGCCGCGTCAGCCGGGCGTTGACGCGGGCGC
TGCAGCGCACCTACATCGACGACACCCTGAGGCCGATGCTGACCGCGGTGGCGCAATGGATCATCCGGATCCTGACCATC
GTGCTGGTGCTGTCGCAGTTTGGTGTGCAGACCGCCAGCATCATCGCGATGCTGGGTGCCGCCGGCCTGGCGATCGGGCT
GGCGTTGCAAGGCACGCTGCAGAACATCGCGGCCGGCATCATGCTGGTGCTGCTGCGGCCGTTCCGCGTCGGGCAGTACA
TCGACGCGCAGGGCGTGGCGGGCACGGTGCGCGAAACCGGCCTCTTCATGACCGAGTTGACGACGGCCGATGGCGTTTGC
ATGCGCGTGCCGAACGGCAAGATCTGGGGCAGCGCCATCACCAACTACAGCGAGAACCCGACGCGCCGCCTGGACATCGA
GGCCATCGTCACGTTCGACAGCGACATTCAGGCGGGCCTCGATGCGCTCAAGGCCATGATGGCCGAGGAGTCACGCCTGC
TGCCCGAGCCGAGGCCCGAAGTCATGGTGACCCGCTACACCGACCGGGGCATCACGCTGAACGCGCGCTACTGGACGAAC
AGCGGCGACTTCTGGAACGTCCAATATGGCTTCTTCGGCCGGCTGCAACAGCGGCTAGAGGATGCCGGTTGCCGCCTGGC
CGTACCCGTGCAGGAGGTACGCAGGCCAGAGAGCGGCGACGCCGCCCGGGCGTAA

Upstream 100 bases:

>100_bases
CGTCACGCAGAAATGGGTACGCTTTTGCTGAAGACCGATCCGGAACGTCTCGGTCCGACGGGTGTCTACGAGCTTTGCCC
TGAAAGGAAGGAGCCGACGG

Downstream 100 bases:

>100_bases
CCCACACGCACGCGAAACGCGGGCAAAACGCGTACAAAACGCGCGCAAGAATCGTACGCAGAAACGGGTACGCTTTCGCG
GCCGCCGCCCCGGCGGCCGT

Product: Small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MDLKFIDAAKVEATWTYLAQFAVSQGLNLLAALAILVGGWWLSGRVSRALTRALQRTYIDDTLRPMLTAVAQWIIRILTI
VLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGIMLVLLRPFRVGQYIDAQGVAGTVRETGLFMTELTTADGVC
MRVPNGKIWGSAITNYSENPTRRLDIEAIVTFDSDIQAGLDALKAMMAEESRLLPEPRPEVMVTRYTDRGITLNARYWTN
SGDFWNVQYGFFGRLQQRLEDAGCRLAVPVQEVRRPESGDAARA

Sequences:

>Translated_284_residues
MDLKFIDAAKVEATWTYLAQFAVSQGLNLLAALAILVGGWWLSGRVSRALTRALQRTYIDDTLRPMLTAVAQWIIRILTI
VLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGIMLVLLRPFRVGQYIDAQGVAGTVRETGLFMTELTTADGVC
MRVPNGKIWGSAITNYSENPTRRLDIEAIVTFDSDIQAGLDALKAMMAEESRLLPEPRPEVMVTRYTDRGITLNARYWTN
SGDFWNVQYGFFGRLQQRLEDAGCRLAVPVQEVRRPESGDAARA
>Mature_284_residues
MDLKFIDAAKVEATWTYLAQFAVSQGLNLLAALAILVGGWWLSGRVSRALTRALQRTYIDDTLRPMLTAVAQWIIRILTI
VLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGIMLVLLRPFRVGQYIDAQGVAGTVRETGLFMTELTTADGVC
MRVPNGKIWGSAITNYSENPTRRLDIEAIVTFDSDIQAGLDALKAMMAEESRLLPEPRPEVMVTRYTDRGITLNARYWTN
SGDFWNVQYGFFGRLQQRLEDAGCRLAVPVQEVRRPESGDAARA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=284, Percent_Identity=38.3802816901408, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787591, Length=222, Percent_Identity=24.7747747747748, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786670, Length=161, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 31168; Mature: 31168

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKFIDAAKVEATWTYLAQFAVSQGLNLLAALAILVGGWWLSGRVSRALTRALQRTYID
CCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DTLRPMLTAVAQWIIRILTIVLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVLLRPFRVGQYIDAQGVAGTVRETGLFMTELTTADGVCMRVPNGKIWGSAITNYSENP
HHHHHHHHHCCCEECCCCCCCHHHHHCCHHEEECCCCCEEEECCCCCCCHHHHCCCCCCC
TRRLDIEAIVTFDSDIQAGLDALKAMMAEESRLLPEPRPEVMVTRYTDRGITLNARYWTN
CEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEECC
SGDFWNVQYGFFGRLQQRLEDAGCRLAVPVQEVRRPESGDAARA
CCCEEEEHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDLKFIDAAKVEATWTYLAQFAVSQGLNLLAALAILVGGWWLSGRVSRALTRALQRTYID
CCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DTLRPMLTAVAQWIIRILTIVLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVLLRPFRVGQYIDAQGVAGTVRETGLFMTELTTADGVCMRVPNGKIWGSAITNYSENP
HHHHHHHHHCCCEECCCCCCCHHHHHCCHHEEECCCCCEEEECCCCCCCHHHHCCCCCCC
TRRLDIEAIVTFDSDIQAGLDALKAMMAEESRLLPEPRPEVMVTRYTDRGITLNARYWTN
CEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEECC
SGDFWNVQYGFFGRLQQRLEDAGCRLAVPVQEVRRPESGDAARA
CCCEEEEHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]