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The map label for this gene is kpsT [H]

Identifier: 94314650

GI number: 94314650

Start: 2491237

End: 2491902

Strand: Reverse

Name: kpsT [H]

Synonym: Rmet_5731

Alternate gene names: 94314650

Gene position: 2491902-2491237 (Counterclockwise)

Preceding gene: 94314651

Following gene: 94314649

Centisome position: 96.58

GC content: 56.16

Gene sequence:

>666_bases
ATGATCGAGCTACGACAGGTCTGGAAGCGCTACAGCGGCCATCACAGTCGCAACTGGGTGCTCCGGGATATCAACCTCTG
TATCCGCCAGGGCGATCGTGTCGGCTTGATTGGTCGCAACGGTGCAGGCAAGTCAACCCTTCTTAACCTGATCGGCGGGA
TGGATCATCCGAGCCGCGGCCAGGTTCGCCGCGACGGCAAGCTTTCATGGACCCTTGGGCTGAACGGAGGCTTCCAAGGT
GCGCTCTCCGGAGAACAGAACGCTCGCTTCGTGTTTCGCATCCACGGCGTGCCGGACCAGGAGATCGCTGAGCGCACGAA
CTTCGTGCATGTTTTCTCCGAGTTGGGCGATTACTTCTTCGCGCCAATGAAAACATACTCGTCCGGGATGCGCTCGCGGC
TTGCCTTCGCTCTATCACTGGCTTTCCGATTCGACACCTACCTCGTCGACGAGCTCATCGCGGTCGGCGATGAGAAATTC
AAGAAAAAGTCGAAGAAGGCATTTGAGGATCTCATTGCTTCCGCGGGGCTGATCATGGTCAGCCACGACGAGGGAACGCT
CAAAAATTTTTGCGAGAAGGGAATCCTTATGAGCAATGGCGCCGCCTACTGGTTCGACGACATTGCAGATGCCCTCTCCG
AGTACAAGAAAAGTATAAACGCATGA

Upstream 100 bases:

>100_bases
ACGAGGGGATCAGCATTTCCTACCTAAGCCTGATATCGGCTGCGTTGGTGACTTTTGGCCTGCTGCTACACGTCCGATTT
CGGCGAGCACTGGAGTCGCG

Downstream 100 bases:

>100_bases
AAACGTTGTCCGGCGTTGCCACCGCGGCCCGCCGCTCCCCTCTTCTAGCCGCCTTCGTATTGTTCAGTCTGATCTCTGGG
CTGTACTGGGGCGTGATCGC

Product: Polysialic acid transport ATP-binding protein kpsT

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MIELRQVWKRYSGHHSRNWVLRDINLCIRQGDRVGLIGRNGAGKSTLLNLIGGMDHPSRGQVRRDGKLSWTLGLNGGFQG
ALSGEQNARFVFRIHGVPDQEIAERTNFVHVFSELGDYFFAPMKTYSSGMRSRLAFALSLAFRFDTYLVDELIAVGDEKF
KKKSKKAFEDLIASAGLIMVSHDEGTLKNFCEKGILMSNGAAYWFDDIADALSEYKKSINA

Sequences:

>Translated_221_residues
MIELRQVWKRYSGHHSRNWVLRDINLCIRQGDRVGLIGRNGAGKSTLLNLIGGMDHPSRGQVRRDGKLSWTLGLNGGFQG
ALSGEQNARFVFRIHGVPDQEIAERTNFVHVFSELGDYFFAPMKTYSSGMRSRLAFALSLAFRFDTYLVDELIAVGDEKF
KKKSKKAFEDLIASAGLIMVSHDEGTLKNFCEKGILMSNGAAYWFDDIADALSEYKKSINA
>Mature_221_residues
MIELRQVWKRYSGHHSRNWVLRDINLCIRQGDRVGLIGRNGAGKSTLLNLIGGMDHPSRGQVRRDGKLSWTLGLNGGFQG
ALSGEQNARFVFRIHGVPDQEIAERTNFVHVFSELGDYFFAPMKTYSSGMRSRLAFALSLAFRFDTYLVDELIAVGDEKF
KKKSKKAFEDLIASAGLIMVSHDEGTLKNFCEKGILMSNGAAYWFDDIADALSEYKKSINA

Specific function: Putative ATP-binding protein, and an energy coupling component for the transport of polysialic acid across the cytoplasmic membrane [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI87081791, Length=212, Percent_Identity=29.2452830188679, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87081709, Length=212, Percent_Identity=26.4150943396226, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786398, Length=236, Percent_Identity=24.5762711864407, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787758, Length=201, Percent_Identity=26.865671641791, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790467, Length=193, Percent_Identity=26.9430051813472, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1789891, Length=229, Percent_Identity=20.9606986899563, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082267, Length=231, Percent_Identity=22.5108225108225, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=194, Percent_Identity=26.8041237113402, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007184, Length=215, Percent_Identity=23.7209302325581, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221500365, Length=215, Percent_Identity=23.7209302325581, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24804; Mature: 24804

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELRQVWKRYSGHHSRNWVLRDINLCIRQGDRVGLIGRNGAGKSTLLNLIGGMDHPSRG
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCC
QVRRDGKLSWTLGLNGGFQGALSGEQNARFVFRIHGVPDQEIAERTNFVHVFSELGDYFF
CEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
APMKTYSSGMRSRLAFALSLAFRFDTYLVDELIAVGDEKFKKKSKKAFEDLIASAGLIMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEE
SHDEGTLKNFCEKGILMSNGAAYWFDDIADALSEYKKSINA
ECCCCHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIELRQVWKRYSGHHSRNWVLRDINLCIRQGDRVGLIGRNGAGKSTLLNLIGGMDHPSRG
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCC
QVRRDGKLSWTLGLNGGFQGALSGEQNARFVFRIHGVPDQEIAERTNFVHVFSELGDYFF
CEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
APMKTYSSGMRSRLAFALSLAFRFDTYLVDELIAVGDEKFKKKSKKAFEDLIASAGLIMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEE
SHDEGTLKNFCEKGILMSNGAAYWFDDIADALSEYKKSINA
ECCCCHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1856162; 8051103 [H]