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The map label for this gene is fumB [H]

Identifier: 94314390

GI number: 94314390

Start: 2216282

End: 2216845

Strand: Reverse

Name: fumB [H]

Synonym: Rmet_5471

Alternate gene names: 94314390

Gene position: 2216845-2216282 (Counterclockwise)

Preceding gene: 94314391

Following gene: 94314389

Centisome position: 85.92

GC content: 58.69

Gene sequence:

>564_bases
ATGGCAACACATCGACTCACCACGCCTCTGACGGAAGAGGCAATTCGCCAACTCAACGTTGGTGATGAGGTACTGCTGTC
TGGCACGATTTACATGGCACGTGACGCTGCCCACAAGCGTTTCGTCGATACGCTGGCGCGCGGCGAACCGCTGCCGGTCG
ACCTCAAAGGACAAGTGGTCTTCTATGGCGGTCCGGGTCCCACCAAGCCTGGTCACGTAATTGGGGTGGTGGCACCGACC
TCGGCCTATCGCATGGACCCCTATGCGACCGTGCTGTTCGAATATGGGGTGAAGGGTGCTATCGGGAAGGGTGACCGGGG
CGACGCCATCAGGGCATCGTGCAAGTCCAACACAGCGGTCTGTTTCTCTGCGATTGGCGGCATTTCGGCCACGTTGTTCT
CGTGCATCAAGTCCGCGGAGATCATTGCCTACGAGGATTTGAAGACAGAGGCCGTCCAGCGCCTCGTCATCGAGGACTTC
CCGTTGCTCGTTACTAACGACGCGCAGGGCCGGGACATGTACGAAGAGGAAGTGACACGCTTCAAGAAGCTGCTGAACAG
CTGA

Upstream 100 bases:

>100_bases
GAGACCTATGCTTGTCACATCACCGCCTTGCCGGTAGCTGTGAACATCCAATGCCACGCTGGCCGCCGCGCCACTGCGGT
TATCTGAGGAGCGGGCCATT

Downstream 100 bases:

>100_bases
CAGCCGGACTTTGTGTACGATAGCGCCTGTCGAAGCCCGGGCGCCCGCCCGGCTTCCTTTATATTGAGCCGGGCGCGCAC
TTGGGCCCGCCTGTTTGGGG

Product: hydro-lyases, Fe-S type, tartrate/fumarate subfamily, beta region

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MATHRLTTPLTEEAIRQLNVGDEVLLSGTIYMARDAAHKRFVDTLARGEPLPVDLKGQVVFYGGPGPTKPGHVIGVVAPT
SAYRMDPYATVLFEYGVKGAIGKGDRGDAIRASCKSNTAVCFSAIGGISATLFSCIKSAEIIAYEDLKTEAVQRLVIEDF
PLLVTNDAQGRDMYEEEVTRFKKLLNS

Sequences:

>Translated_187_residues
MATHRLTTPLTEEAIRQLNVGDEVLLSGTIYMARDAAHKRFVDTLARGEPLPVDLKGQVVFYGGPGPTKPGHVIGVVAPT
SAYRMDPYATVLFEYGVKGAIGKGDRGDAIRASCKSNTAVCFSAIGGISATLFSCIKSAEIIAYEDLKTEAVQRLVIEDF
PLLVTNDAQGRDMYEEEVTRFKKLLNS
>Mature_186_residues
ATHRLTTPLTEEAIRQLNVGDEVLLSGTIYMARDAAHKRFVDTLARGEPLPVDLKGQVVFYGGPGPTKPGHVIGVVAPTS
AYRMDPYATVLFEYGVKGAIGKGDRGDAIRASCKSNTAVCFSAIGGISATLFSCIKSAEIIAYEDLKTEAVQRLVIEDFP
LLVTNDAQGRDMYEEEVTRFKKLLNS

Specific function: It Functions As An Aerobic Enzyme In The Citric Acid Cycle. It Accounts For About 80% Of The Fumarase Activity When The Bacteria Grows Aerobically. [C]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=175, Percent_Identity=33.1428571428571, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790564, Length=179, Percent_Identity=31.8435754189944, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=29.8342541436464, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 20255; Mature: 20124

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHRLTTPLTEEAIRQLNVGDEVLLSGTIYMARDAAHKRFVDTLARGEPLPVDLKGQVV
CCCCCCCCCHHHHHHHHCCCCCCEEEECEEEEHHHHHHHHHHHHHHCCCCCCEECCCCEE
FYGGPGPTKPGHVIGVVAPTSAYRMDPYATVLFEYGVKGAIGKGDRGDAIRASCKSNTAV
EECCCCCCCCCEEEEEECCCCCEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEE
CFSAIGGISATLFSCIKSAEIIAYEDLKTEAVQRLVIEDFPLLVTNDAQGRDMYEEEVTR
EHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
FKKLLNS
HHHHHCC
>Mature Secondary Structure 
ATHRLTTPLTEEAIRQLNVGDEVLLSGTIYMARDAAHKRFVDTLARGEPLPVDLKGQVV
CCCCCCCCHHHHHHHHCCCCCCEEEECEEEEHHHHHHHHHHHHHHCCCCCCEECCCCEE
FYGGPGPTKPGHVIGVVAPTSAYRMDPYATVLFEYGVKGAIGKGDRGDAIRASCKSNTAV
EECCCCCCCCCEEEEEECCCCCEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEE
CFSAIGGISATLFSCIKSAEIIAYEDLKTEAVQRLVIEDFPLLVTNDAQGRDMYEEEVTR
EHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
FKKLLNS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8473853 [H]