Click here to switch to the map view.

The map label for this gene is glpK [H]

Identifier: 94314364

GI number: 94314364

Start: 2195102

End: 2196622

Strand: Reverse

Name: glpK [H]

Synonym: Rmet_5445

Alternate gene names: 94314364

Gene position: 2196622-2195102 (Counterclockwise)

Preceding gene: 94314365

Following gene: 94314363

Centisome position: 85.14

GC content: 66.6

Gene sequence:

>1521_bases
ATGAAAAACAGGAACGTCATCCTGGCGATCGACGAAGGCACGTCTGGTACGCGTGCCGCGGTGGTTGCCAGCGACGGCCA
CGTCTCGTGTCAGCAGTACACCACGCTGCAGGTGGATTCACCGCGTCCCGGCGTGGTGGAGCAGGACGCCAACGTGCTGC
TGGAAAAGACACTGGAAGTCTGCCGCGCCACGCTGGCCGAAGCCGAACGCAACGACTTGTGCATCGTCGCGCTGGCCATC
GCCACGCAGCGCTCCACCGGCGTGCTGTGGGACACCGTAACGGGGCGTGCGCTGGTGCCCGCAATGGTCTGGCAGGACAC
GCGGTACGCGGCGGACCTCGATCAGATGGCATCCACGTGGGACCCGCGCTTGCGCGCCACCGTTGGTCGTCCAGCCGGCG
TGCGCTCGGCTTACCTGTGGGCCGCGCGACATCTGCGGGAGACGCGCGCCGTCGCCGATGCGTGGCGCGATCGCCGGCTT
GCGTTCGGCACCGTCGATAGCTGGCTGCTGTGGCACCTGTCCACCGAACGCGCGTGTGTGACCACACCGACGAATGCCAC
ATCGTGCAGTGCCTATGTTCTTGGTGAACACCGCTACCTGACGGAGTGGGTCGAGGCGCTCGGATTCCCGCAAGAGTTGC
TGCCAGAGTTGCGCCAGGATGCCGACGACTTCGGGCGCACGCGCCGCGACCTGCTTGGCGTCGACGTGCCGATCCTCGCC
TGCGCGGGCGACCAACTGGCGGGCGCAGTCGGCCTCGGTTGTCTCGACGCCGGCCAGTCGATGTGCCTGCACGGCACCGG
CAGCTTTGTTGATCTCGTCATCGGCCCGGACCTGCCCGAGCGCAGCGGGCAGTCGGACAGCACGCTGACGATGACGGCAC
GTCGCTTCGAGGGGGTTTCGCACTTTTCGGTGGAAACGTTCGTGGCTACTACCGGCTCGGCGCTGAACTGGGTTTGCGAC
AAGCTGCACTGGTTCGAAAGCGCGAAGCAGATCAGCGCATTGGCTGCGTCGGTGCCGTCATCGCGCGGCGTGACGTTCAT
TCCCGCGCTGACGGGCTTGCGTGTGCCGCAGATGCGGCCCGAGGCGCGCGCTGCGTTGAACGGGGTGTCGATGGCCACCA
CTCAGGCGGAGATCGCCTACGCAATCCTGGAAGGCATCGCGCATTCCGTGGCGTCCTGCATCGATGCCAACCGCGATATT
GCCGGCGTGCCGGTGCGTGATCTCGTCGTCGGCGGGGGATTGTCGGGCAGCGACGCGCTGCTGCAGATGCAGGCTGACCT
TACGGGCCTGCCGATTCATCGGATGCAGGAGACCGACCGGGCCAGCCTGCGTGGCATTGCCTATCTGGCTGGCGCATCGG
GGCTGATGTGGGATTCGCTGCAGCAAGCCCGCACCACCACGTTGCCGGATGCGGTGTTCATGCCGCGCATCGGCGACGAC
GAACGGGAGGAACGGCGTGCGCTCTGGCACGCGCGTGTCGCTTCCGAGCTTTCCCACGTGCAGGCATTTGCCGCGCATTG
A

Upstream 100 bases:

>100_bases
ACGGCCATTCATCCCGATAACGAAGCCGATGCGCCGCAGTTCCGCAGCGATGCACGCGACGGCGCCGGCTTCATGCATCC
CGCTACAGAGAAGTAACACC

Downstream 100 bases:

>100_bases
CCGATCAGGAGAAGCAAGCAACATGATGGGTTTTCCATCCCGCAGCGCAAACCGCGAGCGCGCCGCCATGACCCGCGTCG
AGCCGCTTCGGCTGGTGCGC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKNRNVILAIDEGTSGTRAAVVASDGHVSCQQYTTLQVDSPRPGVVEQDANVLLEKTLEVCRATLAEAERNDLCIVALAI
ATQRSTGVLWDTVTGRALVPAMVWQDTRYAADLDQMASTWDPRLRATVGRPAGVRSAYLWAARHLRETRAVADAWRDRRL
AFGTVDSWLLWHLSTERACVTTPTNATSCSAYVLGEHRYLTEWVEALGFPQELLPELRQDADDFGRTRRDLLGVDVPILA
CAGDQLAGAVGLGCLDAGQSMCLHGTGSFVDLVIGPDLPERSGQSDSTLTMTARRFEGVSHFSVETFVATTGSALNWVCD
KLHWFESAKQISALAASVPSSRGVTFIPALTGLRVPQMRPEARAALNGVSMATTQAEIAYAILEGIAHSVASCIDANRDI
AGVPVRDLVVGGGLSGSDALLQMQADLTGLPIHRMQETDRASLRGIAYLAGASGLMWDSLQQARTTTLPDAVFMPRIGDD
EREERRALWHARVASELSHVQAFAAH

Sequences:

>Translated_506_residues
MKNRNVILAIDEGTSGTRAAVVASDGHVSCQQYTTLQVDSPRPGVVEQDANVLLEKTLEVCRATLAEAERNDLCIVALAI
ATQRSTGVLWDTVTGRALVPAMVWQDTRYAADLDQMASTWDPRLRATVGRPAGVRSAYLWAARHLRETRAVADAWRDRRL
AFGTVDSWLLWHLSTERACVTTPTNATSCSAYVLGEHRYLTEWVEALGFPQELLPELRQDADDFGRTRRDLLGVDVPILA
CAGDQLAGAVGLGCLDAGQSMCLHGTGSFVDLVIGPDLPERSGQSDSTLTMTARRFEGVSHFSVETFVATTGSALNWVCD
KLHWFESAKQISALAASVPSSRGVTFIPALTGLRVPQMRPEARAALNGVSMATTQAEIAYAILEGIAHSVASCIDANRDI
AGVPVRDLVVGGGLSGSDALLQMQADLTGLPIHRMQETDRASLRGIAYLAGASGLMWDSLQQARTTTLPDAVFMPRIGDD
EREERRALWHARVASELSHVQAFAAH
>Mature_506_residues
MKNRNVILAIDEGTSGTRAAVVASDGHVSCQQYTTLQVDSPRPGVVEQDANVLLEKTLEVCRATLAEAERNDLCIVALAI
ATQRSTGVLWDTVTGRALVPAMVWQDTRYAADLDQMASTWDPRLRATVGRPAGVRSAYLWAARHLRETRAVADAWRDRRL
AFGTVDSWLLWHLSTERACVTTPTNATSCSAYVLGEHRYLTEWVEALGFPQELLPELRQDADDFGRTRRDLLGVDVPILA
CAGDQLAGAVGLGCLDAGQSMCLHGTGSFVDLVIGPDLPERSGQSDSTLTMTARRFEGVSHFSVETFVATTGSALNWVCD
KLHWFESAKQISALAASVPSSRGVTFIPALTGLRVPQMRPEARAALNGVSMATTQAEIAYAILEGIAHSVASCIDANRDI
AGVPVRDLVVGGGLSGSDALLQMQADLTGLPIHRMQETDRASLRGIAYLAGASGLMWDSLQQARTTTLPDAVFMPRIGDD
EREERRALWHARVASELSHVQAFAAH

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=509, Percent_Identity=30.2554027504912, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI189409120, Length=509, Percent_Identity=30.4518664047151, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI42794763, Length=516, Percent_Identity=30.0387596899225, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI41393575, Length=512, Percent_Identity=30.078125, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI88196792, Length=514, Percent_Identity=25.4863813229572, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1790361, Length=499, Percent_Identity=29.2585170340681, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1789987, Length=435, Percent_Identity=25.0574712643678, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=441, Percent_Identity=30.6122448979592, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=24.282982791587, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71986147, Length=296, Percent_Identity=28.0405405405405, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321755, Length=464, Percent_Identity=29.7413793103448, Blast_Score=163, Evalue=8e-41,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=27.1653543307087, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24654573, Length=437, Percent_Identity=27.6887871853547, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655555, Length=514, Percent_Identity=27.8210116731518, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24655560, Length=514, Percent_Identity=27.8210116731518, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24655570, Length=514, Percent_Identity=27.8210116731518, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24655574, Length=514, Percent_Identity=27.8210116731518, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24655565, Length=514, Percent_Identity=27.8210116731518, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19922058, Length=491, Percent_Identity=26.0692464358452, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24652871, Length=477, Percent_Identity=25.9958071278826, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24656367, Length=509, Percent_Identity=25.147347740668, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21357867, Length=514, Percent_Identity=24.7081712062257, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24656372, Length=337, Percent_Identity=26.1127596439169, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54685; Mature: 54685

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRNVILAIDEGTSGTRAAVVASDGHVSCQQYTTLQVDSPRPGVVEQDANVLLEKTLEV
CCCCCEEEEEECCCCCCEEEEEECCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
CRATLAEAERNDLCIVALAIATQRSTGVLWDTVTGRALVPAMVWQDTRYAADLDQMASTW
HHHHHHHCCCCCEEEEEEEEECCCCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCC
DPRLRATVGRPAGVRSAYLWAARHLRETRAVADAWRDRRLAFGTVDSWLLWHLSTERACV
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEE
TTPTNATSCSAYVLGEHRYLTEWVEALGFPQELLPELRQDADDFGRTRRDLLGVDVPILA
ECCCCCCCCEEEEEECHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCEEE
CAGDQLAGAVGLGCLDAGQSMCLHGTGSFVDLVIGPDLPERSGQSDSTLTMTARRFEGVS
ECCCHHHHHHCCHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHCCCC
HFSVETFVATTGSALNWVCDKLHWFESAKQISALAASVPSSRGVTFIPALTGLRVPQMRP
CCCCEEHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCC
EARAALNGVSMATTQAEIAYAILEGIAHSVASCIDANRDIAGVPVRDLVVGGGLSGSDAL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCCCCCE
LQMQADLTGLPIHRMQETDRASLRGIAYLAGASGLMWDSLQQARTTTLPDAVFMPRIGDD
EEEECCCCCCCHHHHCCCHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCC
EREERRALWHARVASELSHVQAFAAH
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNRNVILAIDEGTSGTRAAVVASDGHVSCQQYTTLQVDSPRPGVVEQDANVLLEKTLEV
CCCCCEEEEEECCCCCCEEEEEECCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
CRATLAEAERNDLCIVALAIATQRSTGVLWDTVTGRALVPAMVWQDTRYAADLDQMASTW
HHHHHHHCCCCCEEEEEEEEECCCCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCC
DPRLRATVGRPAGVRSAYLWAARHLRETRAVADAWRDRRLAFGTVDSWLLWHLSTERACV
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEE
TTPTNATSCSAYVLGEHRYLTEWVEALGFPQELLPELRQDADDFGRTRRDLLGVDVPILA
ECCCCCCCCEEEEEECHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCEEE
CAGDQLAGAVGLGCLDAGQSMCLHGTGSFVDLVIGPDLPERSGQSDSTLTMTARRFEGVS
ECCCHHHHHHCCHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHCCCC
HFSVETFVATTGSALNWVCDKLHWFESAKQISALAASVPSSRGVTFIPALTGLRVPQMRP
CCCCEEHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCC
EARAALNGVSMATTQAEIAYAILEGIAHSVASCIDANRDIAGVPVRDLVVGGGLSGSDAL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCCCCCE
LQMQADLTGLPIHRMQETDRASLRGIAYLAGASGLMWDSLQQARTTTLPDAVFMPRIGDD
EEEECCCCCCCHHHHCCCHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCC
EREERRALWHARVASELSHVQAFAAH
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA