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The map label for this gene is ilvD [H]

Identifier: 94314190

GI number: 94314190

Start: 1969851

End: 1971635

Strand: Direct

Name: ilvD [H]

Synonym: Rmet_5271

Alternate gene names: 94314190

Gene position: 1969851-1971635 (Clockwise)

Preceding gene: 94314188

Following gene: 94314191

Centisome position: 76.35

GC content: 65.21

Gene sequence:

>1785_bases
ATGACAACTGACGACTATGACGAATACCAACGTGGAAGAGACAAGATGACGGCTCACAGAAAGACGCCGGACGCACTCCG
CAGCGCGCGCTGGTTCGCGCCTGACGATCTGCGCTCATCCGGGCACCGCTCACGGATCATGCAAATGGGCTACTCGCCCG
ATGAATGGATGGGCAAGCCGCTCATCGCCATCATCAATACGTGGTCCGACATCAACCCGTGCCACGCGCACTTCAAGCAG
CGCGTGGAAGACGTCAAGCGCGGCATCCTGCAGGCCGGTGGTTTCCCCGTGGAGCTACCGGCCATTTCTTTGTCCGAGAG
CTCGGTCAAGCCGACCACCATGCTCTACCGCAACCTGCTGGCGATCGAGGCCGAGGAGCTGATCCGCTCCCACCCGATCG
ACGGGGCCGTGCTGATGGGTGGCTGCGACAAGACCACGCCGGGCCTGCTGATGGGCGCCACCAGCGCCGGCGTGCCGGCG
ATCTACGTGCCGGCCGGGCCAATGCTGCGCGGCAACTACAAGGGCACGGTGCTCGGCTCCGGTTCCGATGGATGGAAGTA
CTGGGACGAACGCCGCGCCGGCAACATCAGCAAGGCCGAGTGGATCGGCATCGAGGGCGGCATCGCGCGCAGCCACGGCA
CGTGCATGACGATGGGCACCGCCAGCACGATGACGGCGATCGCCGAAACCATTGGCATGACGCTGCCGGGTGCGTCGTCG
ATTCCGGCGGCGGACGCCAATCATATCCGCATGTGTTCCGAATCGGGTCGCCGCATCGTGGAGATGGTCTGGGAAGACCT
GACGCCCGCACGCATCCAGACGCACCGTGCGTTCGAAAACGCCATCGCGGTGGCCATGGCCATGGGCTGCTCGACCAACG
CGATCATCCACGTCATCAGCATGGCGCGCCGCGCGGGCCATGACATCGGTCTCGACGATTTCGACCGCATGAGCCGCCAC
GTGCCCGTGATCGCCAACATTCGGCCCAGCGGCGACAAGTACCTGATGGAAGACTTCTTCTACGCGGGCGGGCTGCCCTC
GCTGATGGGCCGTATCCGGGACAAGCTGCATCTCGACACGCTGACCGTCACCGGCCGCACGCTGGGCGAGAACATCGCCG
GGGCCGAGGTCTACAACGACGACGTGATCCGCACGCGCGACAACGCGCTCTACCAGGAAGGCGCGCTGGCCGTCCTGCGC
GGCAACATCGCACCGGACGGCTGCGTGATCAAGCCGAGCGCGTGCGAAAAACGGTTCTTCAAACATACCGGTCCGGCGCT
CGTGTTCGACGACTACCCGTCGATGAAGGCAGCGGTCGAGGACGAAGACCTCGACGTTACGGCCGATCATGTGCTGATCC
TGCGCAATGCGGGGCCGCAGGGCGGGCCAGGCATGCCCGAGTGGGGGATGCTGCCGATTCCGAAGAAGCTCGTGAAGCAG
GGCGTGCGCGACATGCTGCGCATGTCCGACGCGCGGATGAGTGGCACGAGCTACGGCGCCTGCATCCTGCACGTGGCGCC
GGAAGCGTACATCGGCGGGCCGTTCGCGCTGGTGCAAACCGGTGACCTGATCACGGTCGACATCGACCGGCGCAGCATCC
ATCTGGAAGTCTCCGACGAAGAGCTCGAACGCCGCCGCGCGGCATGGCAACCGCCGCCGCCCCGCTACTCGCGCGGCTAC
GGCTGGATGTTCTCTCGCCATATCCGCCAGGCCAACGACGGCTGCGATTTCGATTTCCTGCAAACCGACTTCGGCGAAGC
CGCCGGCGAACCGAGCATCTACTGA

Upstream 100 bases:

>100_bases
ATAGGGCGAATTTTCTGTCGCGCAATCCCAACCGCCACATATCTGACTTGCATTGACGCCCGGTTGCGTCGGTGAGTACA
CTGTGCCAGCCAATAGTCGT

Downstream 100 bases:

>100_bases
ACCCGCCATGTCCGAGCTCACCCCTGCCCTGCGCGCCGCGCTGAAAACCGTCAGCACCGCCACGCTGTGCACCGCGCTGT
TCAAGCGCGGGCTGCGCAAC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MTTDDYDEYQRGRDKMTAHRKTPDALRSARWFAPDDLRSSGHRSRIMQMGYSPDEWMGKPLIAIINTWSDINPCHAHFKQ
RVEDVKRGILQAGGFPVELPAISLSESSVKPTTMLYRNLLAIEAEELIRSHPIDGAVLMGGCDKTTPGLLMGATSAGVPA
IYVPAGPMLRGNYKGTVLGSGSDGWKYWDERRAGNISKAEWIGIEGGIARSHGTCMTMGTASTMTAIAETIGMTLPGASS
IPAADANHIRMCSESGRRIVEMVWEDLTPARIQTHRAFENAIAVAMAMGCSTNAIIHVISMARRAGHDIGLDDFDRMSRH
VPVIANIRPSGDKYLMEDFFYAGGLPSLMGRIRDKLHLDTLTVTGRTLGENIAGAEVYNDDVIRTRDNALYQEGALAVLR
GNIAPDGCVIKPSACEKRFFKHTGPALVFDDYPSMKAAVEDEDLDVTADHVLILRNAGPQGGPGMPEWGMLPIPKKLVKQ
GVRDMLRMSDARMSGTSYGACILHVAPEAYIGGPFALVQTGDLITVDIDRRSIHLEVSDEELERRRAAWQPPPPRYSRGY
GWMFSRHIRQANDGCDFDFLQTDFGEAAGEPSIY

Sequences:

>Translated_594_residues
MTTDDYDEYQRGRDKMTAHRKTPDALRSARWFAPDDLRSSGHRSRIMQMGYSPDEWMGKPLIAIINTWSDINPCHAHFKQ
RVEDVKRGILQAGGFPVELPAISLSESSVKPTTMLYRNLLAIEAEELIRSHPIDGAVLMGGCDKTTPGLLMGATSAGVPA
IYVPAGPMLRGNYKGTVLGSGSDGWKYWDERRAGNISKAEWIGIEGGIARSHGTCMTMGTASTMTAIAETIGMTLPGASS
IPAADANHIRMCSESGRRIVEMVWEDLTPARIQTHRAFENAIAVAMAMGCSTNAIIHVISMARRAGHDIGLDDFDRMSRH
VPVIANIRPSGDKYLMEDFFYAGGLPSLMGRIRDKLHLDTLTVTGRTLGENIAGAEVYNDDVIRTRDNALYQEGALAVLR
GNIAPDGCVIKPSACEKRFFKHTGPALVFDDYPSMKAAVEDEDLDVTADHVLILRNAGPQGGPGMPEWGMLPIPKKLVKQ
GVRDMLRMSDARMSGTSYGACILHVAPEAYIGGPFALVQTGDLITVDIDRRSIHLEVSDEELERRRAAWQPPPPRYSRGY
GWMFSRHIRQANDGCDFDFLQTDFGEAAGEPSIY
>Mature_593_residues
TTDDYDEYQRGRDKMTAHRKTPDALRSARWFAPDDLRSSGHRSRIMQMGYSPDEWMGKPLIAIINTWSDINPCHAHFKQR
VEDVKRGILQAGGFPVELPAISLSESSVKPTTMLYRNLLAIEAEELIRSHPIDGAVLMGGCDKTTPGLLMGATSAGVPAI
YVPAGPMLRGNYKGTVLGSGSDGWKYWDERRAGNISKAEWIGIEGGIARSHGTCMTMGTASTMTAIAETIGMTLPGASSI
PAADANHIRMCSESGRRIVEMVWEDLTPARIQTHRAFENAIAVAMAMGCSTNAIIHVISMARRAGHDIGLDDFDRMSRHV
PVIANIRPSGDKYLMEDFFYAGGLPSLMGRIRDKLHLDTLTVTGRTLGENIAGAEVYNDDVIRTRDNALYQEGALAVLRG
NIAPDGCVIKPSACEKRFFKHTGPALVFDDYPSMKAAVEDEDLDVTADHVLILRNAGPQGGPGMPEWGMLPIPKKLVKQG
VRDMLRMSDARMSGTSYGACILHVAPEAYIGGPFALVQTGDLITVDIDRRSIHLEVSDEELERRRAAWQPPPPRYSRGYG
WMFSRHIRQANDGCDFDFLQTDFGEAAGEPSIY

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=547, Percent_Identity=32.1755027422303, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI2367371, Length=481, Percent_Identity=32.8482328482328, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786464, Length=455, Percent_Identity=31.8681318681319, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788157, Length=528, Percent_Identity=29.7348484848485, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6322476, Length=554, Percent_Identity=31.7689530685921, Blast_Score=272, Evalue=9e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 65199; Mature: 65068

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDDYDEYQRGRDKMTAHRKTPDALRSARWFAPDDLRSSGHRSRIMQMGYSPDEWMGKP
CCCCCHHHHHCCHHHHHHCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHCCCCCHHHCCCC
LIAIINTWSDINPCHAHFKQRVEDVKRGILQAGGFPVELPAISLSESSVKPTTMLYRNLL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCHHHHHHHHHH
AIEAEELIRSHPIDGAVLMGGCDKTTPGLLMGATSAGVPAIYVPAGPMLRGNYKGTVLGS
HHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCEEEECCCCEEECCCCEEEEEC
GSDGWKYWDERRAGNISKAEWIGIEGGIARSHGTCMTMGTASTMTAIAETIGMTLPGASS
CCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCC
IPAADANHIRMCSESGRRIVEMVWEDLTPARIQTHRAFENAIAVAMAMGCSTNAIIHVIS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MARRAGHDIGLDDFDRMSRHVPVIANIRPSGDKYLMEDFFYAGGLPSLMGRIRDKLHLDT
HHHHCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHEEEE
LTVTGRTLGENIAGAEVYNDDVIRTRDNALYQEGALAVLRGNIAPDGCVIKPSACEKRFF
EEEECHHHCCCCCCCEEECCCCEEECCCHHHHCCCEEEEECCCCCCCEEECCHHHHHHHH
KHTGPALVFDDYPSMKAAVEDEDLDVTADHVLILRNAGPQGGPGMPEWGMLPIPKKLVKQ
HCCCCEEEEECCCCCHHCCCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
GVRDMLRMSDARMSGTSYGACILHVAPEAYIGGPFALVQTGDLITVDIDRRSIHLEVSDE
HHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCEEEEECCCEEEEEECCEEEEEEECHH
ELERRRAAWQPPPPRYSRGYGWMFSRHIRQANDGCDFDFLQTDFGEAAGEPSIY
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTDDYDEYQRGRDKMTAHRKTPDALRSARWFAPDDLRSSGHRSRIMQMGYSPDEWMGKP
CCCCHHHHHCCHHHHHHCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHCCCCCHHHCCCC
LIAIINTWSDINPCHAHFKQRVEDVKRGILQAGGFPVELPAISLSESSVKPTTMLYRNLL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCHHHHHHHHHH
AIEAEELIRSHPIDGAVLMGGCDKTTPGLLMGATSAGVPAIYVPAGPMLRGNYKGTVLGS
HHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCEEEECCCCEEECCCCEEEEEC
GSDGWKYWDERRAGNISKAEWIGIEGGIARSHGTCMTMGTASTMTAIAETIGMTLPGASS
CCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCC
IPAADANHIRMCSESGRRIVEMVWEDLTPARIQTHRAFENAIAVAMAMGCSTNAIIHVIS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MARRAGHDIGLDDFDRMSRHVPVIANIRPSGDKYLMEDFFYAGGLPSLMGRIRDKLHLDT
HHHHCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHEEEE
LTVTGRTLGENIAGAEVYNDDVIRTRDNALYQEGALAVLRGNIAPDGCVIKPSACEKRFF
EEEECHHHCCCCCCCEEECCCCEEECCCHHHHCCCEEEEECCCCCCCEEECCHHHHHHHH
KHTGPALVFDDYPSMKAAVEDEDLDVTADHVLILRNAGPQGGPGMPEWGMLPIPKKLVKQ
HCCCCEEEEECCCCCHHCCCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
GVRDMLRMSDARMSGTSYGACILHVAPEAYIGGPFALVQTGDLITVDIDRRSIHLEVSDE
HHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCEEEEECCCEEEEEECCEEEEEEECHH
ELERRRAAWQPPPPRYSRGYGWMFSRHIRQANDGCDFDFLQTDFGEAAGEPSIY
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA