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The map label for this gene is mhpE [C]

Identifier: 94314128

GI number: 94314128

Start: 1903487

End: 1904524

Strand: Direct

Name: mhpE [C]

Synonym: Rmet_5209

Alternate gene names: 94314128

Gene position: 1903487-1904524 (Clockwise)

Preceding gene: 94314127

Following gene: 94314129

Centisome position: 73.78

GC content: 66.76

Gene sequence:

>1038_bases
ATGACCGACAAGAAACTTTATATCTCCGATGTGACCCTGCGTGATGGTAGCCACGCGATCCGTCACCAGTACTCGGTTCC
GCAGGTGCGTGCCATCGCACGCGCGCTCGATGCGGCAGGCGTGGACTCGATCGAGGTGGCGCACGGCGACGGCCTGGCTG
GCTCCAGCTTCAACTACGGATTCGGCGCCCACACCGACGTGGAATGGATCGCGGCAGTGGCGGAGTCCGTGCAGCGCGCA
GCGGTGGCCACGCTGCTGCTGCCGGGCATCGGTACCGTGCACGACTTGCGCGAAGCCTACGCCGCCGGCGCACGCGTGGT
CAGGGTGGCCACGCACTGCACCGAGGCCGACACGGCGCGGCAGCATATCGAGACCGCGCGGTCGATGGGGATGAATGTCG
CCGGCTTCCTGATGATGAGCCACATGATCCCGCCCGACAGGCTGGCCGGCCAGGCAAAGCTGATGGAAAGTTACGGCGCG
CATTGTGTCTACGTGGTGGACTCCGGCGGCGCCTTGACCATGGACGGCGTGCGCGCGCGCTTTCGTGCGTTCAAAGACGT
GCTCGATCCAAAGACTGAGACCGGCATGCACGCGCACCACAACCTCAGCCTGGGCGTTGCCAACAGCATCGTCGCGGTTG
AGGAAGGTTGCGACCGCATCGATGCGAGCCTGGCCGGCATGGGCGCGGGCGCGGGCAATGCGCCGCTGGAGGTCTTTATT
GCCGCTGCCGAACGCATGGGATGGCACCACGGCTGCGATCTCTACCAGTTGATGGACGCCGCCGACGACATCGTGCGCCC
GCTGCAGGATCGCCCCGTGCGCGTGGACCGCGAAACGCTGGCGCTCGGCTACGCCGGCGTGTATTCGAGCTTCCTGCGCC
ACGCGGAGAGCGCGGCCGGCAAGTACGGCCTGAAGACCGTCGATATCCTGGTCGAGCTGGGACGCCGCCGCATGGTGGGC
GGGCAGGAAGACATGATCGTCGATGTGGCGCTCGACCTGCAGCGGTCGGGCGGCGCGAAGAGCAGGGAGGCAGCATGA

Upstream 100 bases:

>100_bases
CTGGACATCATGACGAGCGCCGCGCTCGCATGCGCCGAACGCGTCGCACAGGCATTGGGCAATCATCATGCCCTGTCGGC
AGCAAGCTAAGGAGCCGGTC

Downstream 100 bases:

>100_bases
GCCATCACGCCGCCATTGCAGGCGCCCTGGACGCTGCCGCGCGCGACGCCGCGGCGATCGGCCAGTTCAGCGCGGCCTCG
CCGTTCACGATTGACGAGGC

Product: 4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase

Products: NA

Alternate protein names: HOA 2; 4-hydroxy-2-keto-pentanoic acid aldolase 2; 4-hydroxy-2-oxopentanoate aldolase 2

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTDKKLYISDVTLRDGSHAIRHQYSVPQVRAIARALDAAGVDSIEVAHGDGLAGSSFNYGFGAHTDVEWIAAVAESVQRA
AVATLLLPGIGTVHDLREAYAAGARVVRVATHCTEADTARQHIETARSMGMNVAGFLMMSHMIPPDRLAGQAKLMESYGA
HCVYVVDSGGALTMDGVRARFRAFKDVLDPKTETGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAAERMGWHHGCDLYQLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAESAAGKYGLKTVDILVELGRRRMVG
GQEDMIVDVALDLQRSGGAKSREAA

Sequences:

>Translated_345_residues
MTDKKLYISDVTLRDGSHAIRHQYSVPQVRAIARALDAAGVDSIEVAHGDGLAGSSFNYGFGAHTDVEWIAAVAESVQRA
AVATLLLPGIGTVHDLREAYAAGARVVRVATHCTEADTARQHIETARSMGMNVAGFLMMSHMIPPDRLAGQAKLMESYGA
HCVYVVDSGGALTMDGVRARFRAFKDVLDPKTETGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAAERMGWHHGCDLYQLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAESAAGKYGLKTVDILVELGRRRMVG
GQEDMIVDVALDLQRSGGAKSREAA
>Mature_344_residues
TDKKLYISDVTLRDGSHAIRHQYSVPQVRAIARALDAAGVDSIEVAHGDGLAGSSFNYGFGAHTDVEWIAAVAESVQRAA
VATLLLPGIGTVHDLREAYAAGARVVRVATHCTEADTARQHIETARSMGMNVAGFLMMSHMIPPDRLAGQAKLMESYGAH
CVYVVDSGGALTMDGVRARFRAFKDVLDPKTETGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFIA
AAERMGWHHGCDLYQLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAESAAGKYGLKTVDILVELGRRRMVGG
QEDMIVDVALDLQRSGGAKSREAA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=336, Percent_Identity=77.9761904761905, Blast_Score=523, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA2_RALME (Q1LCQ8)

Other databases:

- EMBL:   CP000353
- RefSeq:   YP_587337.1
- ProteinModelPortal:   Q1LCQ8
- SMR:   Q1LCQ8
- STRING:   Q1LCQ8
- GeneID:   4042070
- GenomeReviews:   CP000353_GR
- KEGG:   rme:Rmet_5209
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   RRAYDIG
- ProtClustDB:   PRK08195
- BioCyc:   RMET266264:RMET_5209-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36821; Mature: 36690

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 168-168 BINDING 197-197 BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKLYISDVTLRDGSHAIRHQYSVPQVRAIARALDAAGVDSIEVAHGDGLAGSSFNYG
CCCCEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
FGAHTDVEWIAAVAESVQRAAVATLLLPGIGTVHDLREAYAAGARVVRVATHCTEADTAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCHHHHH
QHIETARSMGMNVAGFLMMSHMIPPDRLAGQAKLMESYGAHCVYVVDSGGALTMDGVRAR
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCEEEHHHHHHH
FRAFKDVLDPKTETGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AAAERMGWHHGCDLYQLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAESAAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHCC
KYGLKTVDILVELGRRRMVGGQEDMIVDVALDLQRSGGAKSREAA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCCCCCCCC
>Mature Secondary Structure 
TDKKLYISDVTLRDGSHAIRHQYSVPQVRAIARALDAAGVDSIEVAHGDGLAGSSFNYG
CCCEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
FGAHTDVEWIAAVAESVQRAAVATLLLPGIGTVHDLREAYAAGARVVRVATHCTEADTAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCHHHHH
QHIETARSMGMNVAGFLMMSHMIPPDRLAGQAKLMESYGAHCVYVVDSGGALTMDGVRAR
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCEEEHHHHHHH
FRAFKDVLDPKTETGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AAAERMGWHHGCDLYQLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAESAAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHCC
KYGLKTVDILVELGRRRMVGGQEDMIVDVALDLQRSGGAKSREAA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA