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The map label for this gene is amnE [H]

Identifier: 94314129

GI number: 94314129

Start: 1904521

End: 1905285

Strand: Direct

Name: amnE [H]

Synonym: Rmet_5210

Alternate gene names: 94314129

Gene position: 1904521-1905285 (Clockwise)

Preceding gene: 94314128

Following gene: 94314130

Centisome position: 73.82

GC content: 66.93

Gene sequence:

>765_bases
ATGAGCCATCACGCCGCCATTGCAGGCGCCCTGGACGCTGCCGCGCGCGACGCCGCGGCGATCGGCCAGTTCAGCGCGGC
CTCGCCGTTCACGATTGACGAGGCCTACGGCATCCAGGCCATGTCGATCGAACACCGCTGCCAGCGCGGCGAACGCCGCG
TCGGCATCAAGCTGGGTTTCACCAGCCGCGCCAAGATGGTCCAGATGGGCGTGGATAGCCTGATCTGGGGATGGCTGACC
GACGCCATGATCGAGGAAGACGGCGGCCGCGTGGATCTGGAACGCTATATCCATCCGCGCGCCGAACCGGAGGTCTGCTT
TCTGACCCGCCGCGACATCACTGGCCCGCTGACCCTGCTGGAGAGTGTCGACTACCTGGAGGCCGTGGCGCCGGCGATCG
AGATCATCGACTCGCGCTACCGCGATTTCCGCTTCACGCTGGAAGATGTCGTGGCCGACAACTGCTCGTCGGCGGGACTG
GTCGTCGGCCCGTGGTCGCGCAATGTTGATGTACTGGCCAATCTCGGCGTGGTGCTGCGCATCGACGGCCGCACCAGGCA
AACGGGTTCGACCGCCGCCATTCTCGGACATCCGCTGCGCAGCCTCGTACAGGCATCGCACGTGCTGACGCGCGCCGGCG
CAGTGCTGCCAGCCGGCAGCCTGATCATGGCGGGAGCGGCCACGGCTGCGGAAGCGTTCGTGCGCGGCAACCATGTTTCG
GCAACGATAGGCGCGCTTGGCCGCGCTTCTTTCCTGGCGGCATGA

Upstream 100 bases:

>100_bases
AGCTGGGACGCCGCCGCATGGTGGGCGGGCAGGAAGACATGATCGTCGATGTGGCGCTCGACCTGCAGCGGTCGGGCGGC
GCGAAGAGCAGGGAGGCAGC

Downstream 100 bases:

>100_bases
CGCCATGCCTGCCCGGCTACCGGGCAGGCCTTCACAACGAAAAGAGCAAACATGCAGGCAAACTCCGCTTCATCAGTGGT
GGCCGGAAAGGCCACGCCAC

Product: 4-oxalocrotonate decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSHHAAIAGALDAAARDAAAIGQFSAASPFTIDEAYGIQAMSIEHRCQRGERRVGIKLGFTSRAKMVQMGVDSLIWGWLT
DAMIEEDGGRVDLERYIHPRAEPEVCFLTRRDITGPLTLLESVDYLEAVAPAIEIIDSRYRDFRFTLEDVVADNCSSAGL
VVGPWSRNVDVLANLGVVLRIDGRTRQTGSTAAILGHPLRSLVQASHVLTRAGAVLPAGSLIMAGAATAAEAFVRGNHVS
ATIGALGRASFLAA

Sequences:

>Translated_254_residues
MSHHAAIAGALDAAARDAAAIGQFSAASPFTIDEAYGIQAMSIEHRCQRGERRVGIKLGFTSRAKMVQMGVDSLIWGWLT
DAMIEEDGGRVDLERYIHPRAEPEVCFLTRRDITGPLTLLESVDYLEAVAPAIEIIDSRYRDFRFTLEDVVADNCSSAGL
VVGPWSRNVDVLANLGVVLRIDGRTRQTGSTAAILGHPLRSLVQASHVLTRAGAVLPAGSLIMAGAATAAEAFVRGNHVS
ATIGALGRASFLAA
>Mature_253_residues
SHHAAIAGALDAAARDAAAIGQFSAASPFTIDEAYGIQAMSIEHRCQRGERRVGIKLGFTSRAKMVQMGVDSLIWGWLTD
AMIEEDGGRVDLERYIHPRAEPEVCFLTRRDITGPLTLLESVDYLEAVAPAIEIIDSRYRDFRFTLEDVVADNCSSAGLV
VGPWSRNVDVLANLGVVLRIDGRTRQTGSTAAILGHPLRSLVQASHVLTRAGAVLPAGSLIMAGAATAAEAFVRGNHVSA
TIGALGRASFLAA

Specific function: Involved in the modified meta-cleavage pathway for the 2-aminophenol catabolism [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=216, Percent_Identity=31.4814814814815, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.1.1.77 [H]

Molecular weight: Translated: 27022; Mature: 26891

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHHAAIAGALDAAARDAAAIGQFSAASPFTIDEAYGIQAMSIEHRCQRGERRVGIKLGF
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCEEEEHHHHHHHCCCCEEEEEECC
TSRAKMVQMGVDSLIWGWLTDAMIEEDGGRVDLERYIHPRAEPEVCFLTRRDITGPLTLL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCEEEEECCCCCCHHHHH
ESVDYLEAVAPAIEIIDSRYRDFRFTLEDVVADNCSSAGLVVGPWSRNVDVLANLGVVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHCCCCEEEE
IDGRTRQTGSTAAILGHPLRSLVQASHVLTRAGAVLPAGSLIMAGAATAAEAFVRGNHVS
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
ATIGALGRASFLAA
EHHHHHHHHHHHCC
>Mature Secondary Structure 
SHHAAIAGALDAAARDAAAIGQFSAASPFTIDEAYGIQAMSIEHRCQRGERRVGIKLGF
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCEEEEHHHHHHHCCCCEEEEEECC
TSRAKMVQMGVDSLIWGWLTDAMIEEDGGRVDLERYIHPRAEPEVCFLTRRDITGPLTLL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCEEEEECCCCCCHHHHH
ESVDYLEAVAPAIEIIDSRYRDFRFTLEDVVADNCSSAGLVVGPWSRNVDVLANLGVVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHCCCCEEEE
IDGRTRQTGSTAAILGHPLRSLVQASHVLTRAGAVLPAGSLIMAGAATAAEAFVRGNHVS
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
ATIGALGRASFLAA
EHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9169437; 11081795 [H]