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The map label for this gene is gyaR [H]

Identifier: 94314117

GI number: 94314117

Start: 1893352

End: 1894305

Strand: Reverse

Name: gyaR [H]

Synonym: Rmet_5198

Alternate gene names: 94314117

Gene position: 1894305-1893352 (Counterclockwise)

Preceding gene: 94314132

Following gene: 94314113

Centisome position: 73.42

GC content: 69.5

Gene sequence:

>954_bases
ATGTCGCTGGCGTGCATGAAAGACGAAGGGGAGGTGATCGTGCTGACGCGGCTGCCACTGGATCAGGACTGGCTGCGGGC
GCATGCGCCGCGTTACCGGTTCGTGCCGTGGACAGCGGTGGACGAGGCGCAGCGTGTGCAGGCGCGCGCAGTGCTGACCA
ATGGTTCCACGGGACTGACCGGGGCCGAGATGGCCGCATTGCCGGCGCTGGAACTGGTCGCGAGCTTTGGGGCCGGCTAC
GAGAACATCGAACGGGATGCGGCGCGGATGCGTGGCGTGCGCGTGTGCCATGCGCCGGATACCAATAGCCAGGTCGTGGC
GGACCACGCGCTGGCGATGATGCTGGCGTGGTCGCGCGGCATTGCGATGCTCGATCGCGGCCTGAAGGCGGGGCAATGGG
ATGCGCTGCGCGCGCCGCGGCCGGGCGTGCGCGGCAAGACCCTGGGCATCGTCGGGCTTGGCAATATCGGCCAGCGTCTG
GCCGCGCTGGCAGAGGCCGTGGGCATGCGCGTGGCCTACCTGCGCCGCAGTGGCGCTCTGCAGCCGAACCGCACGCCGTA
TGGCGACGTGCGTTCGCTGGCGGCCGCAAGCGATGTGTTGGCATTGACCTGTCCCGGCGGTCCGGCCACTCGGCATCTGG
TCGACGCGGAGGTACTGCGCGCGCTGGGTCCCGACGGCCTGCTTGTCAACGTGGCGCGTGGCAGCGTGGTGGATACCGAG
GCGCTGGTACAGGCACTGCGACGCGGCGAGCTGGGTGGTGCCGCACTCGACGTGTTGGAAGCCGAGCCCCTGGTGCCCGA
GGCGTTTCGCGAGATGGATCAGGTCATCCTGACGCCGCACATGGCGGGCCGTTCGCCCGAAACGCGCGTGGCCCAGCACG
AGGCGCTGCTGCGTAATCTGGACGGATGGTTCCGTCACGCTGCGCCGGCCCATCCGGTCGATCTGGCAGGCTGA

Upstream 100 bases:

>100_bases
TTTTCTATGTGTATACATGTCAAGGAGTAAGTGATACTTTTCATCGTGTACCGTGTATCGACGCGGAACCCATCAACAAC
GAGCCAAAGGGAGCGAGGCA

Downstream 100 bases:

>100_bases
GCGCGACGCCGCCGGCGCGCATGTGGCAGCCGGCGCGTCGGCTGCCACTAGTTGCGCTGGCCCGGTGCCGCAAGCTGCAG
GATCCAGTGCGCGAAGCGTG

Product: D-3-phosphoglycerate dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSLACMKDEGEVIVLTRLPLDQDWLRAHAPRYRFVPWTAVDEAQRVQARAVLTNGSTGLTGAEMAALPALELVASFGAGY
ENIERDAARMRGVRVCHAPDTNSQVVADHALAMMLAWSRGIAMLDRGLKAGQWDALRAPRPGVRGKTLGIVGLGNIGQRL
AALAEAVGMRVAYLRRSGALQPNRTPYGDVRSLAAASDVLALTCPGGPATRHLVDAEVLRALGPDGLLVNVARGSVVDTE
ALVQALRRGELGGAALDVLEAEPLVPEAFREMDQVILTPHMAGRSPETRVAQHEALLRNLDGWFRHAAPAHPVDLAG

Sequences:

>Translated_317_residues
MSLACMKDEGEVIVLTRLPLDQDWLRAHAPRYRFVPWTAVDEAQRVQARAVLTNGSTGLTGAEMAALPALELVASFGAGY
ENIERDAARMRGVRVCHAPDTNSQVVADHALAMMLAWSRGIAMLDRGLKAGQWDALRAPRPGVRGKTLGIVGLGNIGQRL
AALAEAVGMRVAYLRRSGALQPNRTPYGDVRSLAAASDVLALTCPGGPATRHLVDAEVLRALGPDGLLVNVARGSVVDTE
ALVQALRRGELGGAALDVLEAEPLVPEAFREMDQVILTPHMAGRSPETRVAQHEALLRNLDGWFRHAAPAHPVDLAG
>Mature_316_residues
SLACMKDEGEVIVLTRLPLDQDWLRAHAPRYRFVPWTAVDEAQRVQARAVLTNGSTGLTGAEMAALPALELVASFGAGYE
NIERDAARMRGVRVCHAPDTNSQVVADHALAMMLAWSRGIAMLDRGLKAGQWDALRAPRPGVRGKTLGIVGLGNIGQRLA
ALAEAVGMRVAYLRRSGALQPNRTPYGDVRSLAAASDVLALTCPGGPATRHLVDAEVLRALGPDGLLVNVARGSVVDTEA
LVQALRRGELGGAALDVLEAEPLVPEAFREMDQVILTPHMAGRSPETRVAQHEALLRNLDGWFRHAAPAHPVDLAG

Specific function: Serine biosynthesis; first step. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=271, Percent_Identity=30.6273062730627, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4557497, Length=271, Percent_Identity=30.6273062730627, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI145580578, Length=254, Percent_Identity=31.1023622047244, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4557499, Length=254, Percent_Identity=31.1023622047244, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI145580575, Length=254, Percent_Identity=31.1023622047244, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI23308577, Length=253, Percent_Identity=28.0632411067194, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI6912396, Length=232, Percent_Identity=28.0172413793103, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI1789279, Length=239, Percent_Identity=35.5648535564854, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI87082289, Length=272, Percent_Identity=32.3529411764706, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787645, Length=240, Percent_Identity=27.0833333333333, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788660, Length=265, Percent_Identity=30.9433962264151, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081824, Length=182, Percent_Identity=29.1208791208791, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=247, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25147481, Length=308, Percent_Identity=27.5974025974026, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=276, Percent_Identity=30.4347826086957, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=248, Percent_Identity=31.8548387096774, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=302, Percent_Identity=27.8145695364238, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321253, Length=157, Percent_Identity=31.8471337579618, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324964, Length=281, Percent_Identity=24.1992882562278, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571528, Length=247, Percent_Identity=31.1740890688259, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24646446, Length=244, Percent_Identity=32.3770491803279, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24646448, Length=244, Percent_Identity=32.3770491803279, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24646452, Length=244, Percent_Identity=32.3770491803279, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24646450, Length=244, Percent_Identity=32.3770491803279, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI62472511, Length=240, Percent_Identity=32.9166666666667, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19921140, Length=277, Percent_Identity=28.5198555956679, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574286, Length=257, Percent_Identity=28.0155642023346, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45552429, Length=246, Percent_Identity=26.0162601626016, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585514, Length=246, Percent_Identity=26.0162601626016, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28574282, Length=246, Percent_Identity=26.0162601626016, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28574284, Length=246, Percent_Identity=26.0162601626016, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45551003, Length=246, Percent_Identity=26.0162601626016, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585516, Length=236, Percent_Identity=24.1525423728814, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 33832; Mature: 33701

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLACMKDEGEVIVLTRLPLDQDWLRAHAPRYRFVPWTAVDEAQRVQARAVLTNGSTGLT
CCCEEECCCCCEEEEEECCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHEEEECCCCCCC
GAEMAALPALELVASFGAGYENIERDAARMRGVRVCHAPDTNSQVVADHALAMMLAWSRG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
IAMLDRGLKAGQWDALRAPRPGVRGKTLGIVGLGNIGQRLAALAEAVGMRVAYLRRSGAL
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
QPNRTPYGDVRSLAAASDVLALTCPGGPATRHLVDAEVLRALGPDGLLVNVARGSVVDTE
CCCCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEECHH
ALVQALRRGELGGAALDVLEAEPLVPEAFREMDQVILTPHMAGRSPETRVAQHEALLRNL
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHH
DGWFRHAAPAHPVDLAG
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SLACMKDEGEVIVLTRLPLDQDWLRAHAPRYRFVPWTAVDEAQRVQARAVLTNGSTGLT
CCEEECCCCCEEEEEECCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHEEEECCCCCCC
GAEMAALPALELVASFGAGYENIERDAARMRGVRVCHAPDTNSQVVADHALAMMLAWSRG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
IAMLDRGLKAGQWDALRAPRPGVRGKTLGIVGLGNIGQRLAALAEAVGMRVAYLRRSGAL
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
QPNRTPYGDVRSLAAASDVLALTCPGGPATRHLVDAEVLRALGPDGLLVNVARGSVVDTE
CCCCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEECHH
ALVQALRRGELGGAALDVLEAEPLVPEAFREMDQVILTPHMAGRSPETRVAQHEALLRNL
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHH
DGWFRHAAPAHPVDLAG
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA