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The map label for this gene is frmA [H]

Identifier: 94313706

GI number: 94313706

Start: 1428335

End: 1429441

Strand: Direct

Name: frmA [H]

Synonym: Rmet_4784

Alternate gene names: 94313706

Gene position: 1428335-1429441 (Clockwise)

Preceding gene: 94313705

Following gene: 94313707

Centisome position: 55.36

GC content: 64.32

Gene sequence:

>1107_bases
ATGAAAACCAAAGCCGCCATCGCCTGGAAAGCCGGTGCACCACTCACCATCGAAGAGGTGGACCTCGACGGTCCGCGCGC
GGGCGAAGTCCTCGTGGAGATCAAGGCCACCGGCATCTGCCATACGGACTACTACACGCTGTCCGGCGCGGACCCCGAAG
GCATTTTCCCCGCCATCCTGGGCCATGAAGGCGCGGGCATCGTCACCGACGTTGGTCCGGGCGTCACGTCGCTCAAGCCG
GGCGATCACGTGATTCCGCTCTACACGCCGGAATGCCGTCAGTGCAAGTTCTGCCTGTCGCGCAAGACCAACCTGTGCCA
GGCCATCCGCGCCACGCAGGGCAAGGGCCTCATGCCGGATGGCACCTCGCGCTTCTCGATCGATGGCAAGCCGATCTTCC
ACTACATGGGCACGTCGACGTTCGCCAACCATATCGTGGTGCCCGAAATCGCGCTGGCGAAGATCCGCCCCGACGCACCG
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTGACCACGGGCGTGGGCGCGGTGATCTTCACGGCCAAGGTGGAAGCCGG
CGCGAACGTGGTGGTGTTCGGCCTGGGCGGCATCGGCCTGAACGTGATCCAGGGTGCAAAGATGGTGGGCGCGGACAAGA
TCATCGGCGTGGACCTCAATCCCGCGCGCGAGGCGATGGCACGCAAGTTCGGCATGACCCACTTCGTGAATCCGAAGGAT
GTCGACAACGTGGTGGACCACATCGTCCAACTGACCGACGGCGGCGCCGACTACTCGTTCGAGTGCATCGGCAACACGCA
GGTCATGCGTCAGGCGCTGGAGTGCTGCCACAAGGGCTGGGGCAAGTCGATCATCATCGGCGTGGCCGAAGCGGGCGCTG
AAATCTCCACCCGTCCGTTCCAGCTCGTCACGGGCCGCGAATGGAAGGGCTCGGCCTTCGGCGGCGCGCGCGGGCGCACC
GATGTGCCCAAGATCGTCGACTGGTACATGGACGGCAAGCTGAACATCGACGACCTGATCACGCATACGCTGCCGCTCGA
CCGCATCAACGAGGGCTTCGACCTGATGAAGCGCGGCGAATCGATCCGCTCGGTGGTCCTGTACTGA

Upstream 100 bases:

>100_bases
GTCTGGCTCGTGTGCGCGGTTCGGCTTATAGTCGTGGCCTTGGTATTAGCCTGTCACGCGGACACGTGCCGCCTCAATTC
ATTCGCTCGGGAGTTGCTTC

Downstream 100 bases:

>100_bases
GGACGCGATCCATGGAACTGCTGTCAGAACACGGCTGCCACGGCGGCGTCCAGCGCTTCTACCAGCACCAGTCGGTGGCC
ATCGGGCTGCCGATGCGGTT

Product: alcohol dehydrogenase, zinc-binding

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTIEEVDLDGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGPGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFVNPKD
VDNVVDHIVQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTIEEVDLDGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGPGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFVNPKD
VDNVVDHIVQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGAPLTIEEVDLDGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGPGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFVNPKD
VDNVVDHIVQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.3079019073569, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.8609625668449, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.070460704607, Blast_Score=355, Evalue=3e-98,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.7994579945799, Blast_Score=351, Evalue=5e-97,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=45.9239130434783, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.5284552845528, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=45.4054054054054, Blast_Score=342, Evalue=2e-94,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=45.4054054054054, Blast_Score=342, Evalue=2e-94,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=45.983379501385, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI156627571, Length=358, Percent_Identity=25.4189944134078, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=59.0785907859079, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87082125, Length=382, Percent_Identity=26.7015706806283, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788073, Length=360, Percent_Identity=26.9444444444444, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790045, Length=378, Percent_Identity=28.3068783068783, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=28.3625730994152, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI226510992, Length=373, Percent_Identity=23.8605898123324, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=25.8426966292135, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786825, Length=379, Percent_Identity=25.065963060686, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=25.0681198910082, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=55.7640750670241, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=55.4666666666667, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=55.6851311953353, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17562584, Length=366, Percent_Identity=27.8688524590164, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17562876, Length=357, Percent_Identity=28.0112044817927, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562582, Length=355, Percent_Identity=25.3521126760563, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=372, Percent_Identity=25.8064516129032, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562878, Length=358, Percent_Identity=25.6983240223464, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.9865229110512, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=28.9544235924933, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=370, Percent_Identity=26.4864864864865, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324486, Length=374, Percent_Identity=26.2032085561497, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=366, Percent_Identity=26.5027322404372, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=365, Percent_Identity=25.4794520547945, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=365, Percent_Identity=28.2191780821918, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=365, Percent_Identity=28.2191780821918, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319257, Length=361, Percent_Identity=24.0997229916898, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=60.8695652173913, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17137530, Length=342, Percent_Identity=28.3625730994152, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39362; Mature: 39362

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLDGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGIVTDVGPGVTSLKPGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVIF
CCCEEEECCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFVNPKD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCCCCCCCHH
VDNVVDHIVQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPF
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
HHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLDGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGIVTDVGPGVTSLKPGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVIF
CCCEEEECCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFVNPKD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCCCCCCCHH
VDNVVDHIVQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPF
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]