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The map label for this gene is hyfC [H]

Identifier: 94313592

GI number: 94313592

Start: 1302187

End: 1303134

Strand: Reverse

Name: hyfC [H]

Synonym: Rmet_4670

Alternate gene names: 94313592

Gene position: 1303134-1302187 (Counterclockwise)

Preceding gene: 94313593

Following gene: 94313591

Centisome position: 50.51

GC content: 61.29

Gene sequence:

>948_bases
ATGAGCGCAACCGGCGTGCTGGCACAACTGCTCGAGATAGTGATCTCGATTGTCCTGGCGCCGTTGCTTTCCGGCTGGGT
CAATGTGTGGCGTGCCTGGCTGCAGAACAAGTCCGCGCCGAGCTTCTGGCAGCCCTATCGCATGCTGCACAAGCTCCTCA
ACAAGGAATCGGTGGTGGCTGACAATGCCTCGCCGCTATTCCGCGTCACCCCCTATGTGGTGTTTGGCTGCATGACACTG
GCGTGTTCCATCATCCCCACATTGTCTACCGACCTGCCGCTGGCGCCCGCCGCCGATGCCATCGCCCTGGTTAGCTTGTT
CGCGCTGGCACGGGTGTTCATCTCGCTGGCTGCCATGGATGTTGGCACGGCCTTTGGCACGATGGGTGCGCGCCGAGAGA
TGCTGATCGGCTTCTTGGCGGAGCCGGCGTTGCTGATGGTGCTGTTCTCGGCCTCGCTTATCTCTCACTCGACCTCGCTC
GCCACGATTGTCGAGACGCTGGCCCATCGTGAGCTGACCATCTATCCCGGCATGGCCTTCGCCGGTGTCGCATTCACCAT
GGTGTCACTGGCCGAAAACGCGCGCGTGCCGGTGGACAATCCCGCTACTCACCTGGAGCTGACCATGATCCATGAAGCGT
TGATCCTGGAATACTCGGGGCGGCATCTGGCGCTGCTGGAGTGGGCGGCCAGTCTCAAATTGTTCGCATACTCCTGTATC
GGGCTGGCGCTGTTTATTCCCTGGGGCGTGGGCGAGGCCAATGCACCGCTCTCGGTGTTGCTGGCATTGCCGGCACTGAT
GTTAAAACTGCTGTTGGGCGGGTTCCTGCTGGCGCTGCTGGAGACCGTCAACGCCAAGATGCGGATATTCCGTGTACCCG
AGTTCCTCGCCACCGCTTTCCTGCTGGCGGTCATCGGCCTGCTTGTGCATCTGCTCCTGGTCCACTGA

Upstream 100 bases:

>100_bases
GTCGGTGCAATTCAGCAGGGGCGCATTTCAGCGTATCTGCTGTTCAGCTTTCTGACGTTGATTGCCGTGCTGCTGGCGGT
CATGCGGTGAGGCCATACCC

Downstream 100 bases:

>100_bases
TATGAGCATGCTACTCGCCCAGTTTGTCAACCTGCTCGGCGCGGTCCTGCTGATACTCGCGTTTGCGATGATTTCGCAGC
GCCGCATCCTGTCACTGATT

Product: hydrogenase-4 subunit C

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSATGVLAQLLEIVISIVLAPLLSGWVNVWRAWLQNKSAPSFWQPYRMLHKLLNKESVVADNASPLFRVTPYVVFGCMTL
ACSIIPTLSTDLPLAPAADAIALVSLFALARVFISLAAMDVGTAFGTMGARREMLIGFLAEPALLMVLFSASLISHSTSL
ATIVETLAHRELTIYPGMAFAGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEYSGRHLALLEWAASLKLFAYSCI
GLALFIPWGVGEANAPLSVLLALPALMLKLLLGGFLLALLETVNAKMRIFRVPEFLATAFLLAVIGLLVHLLLVH

Sequences:

>Translated_315_residues
MSATGVLAQLLEIVISIVLAPLLSGWVNVWRAWLQNKSAPSFWQPYRMLHKLLNKESVVADNASPLFRVTPYVVFGCMTL
ACSIIPTLSTDLPLAPAADAIALVSLFALARVFISLAAMDVGTAFGTMGARREMLIGFLAEPALLMVLFSASLISHSTSL
ATIVETLAHRELTIYPGMAFAGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEYSGRHLALLEWAASLKLFAYSCI
GLALFIPWGVGEANAPLSVLLALPALMLKLLLGGFLLALLETVNAKMRIFRVPEFLATAFLLAVIGLLVHLLLVH
>Mature_314_residues
SATGVLAQLLEIVISIVLAPLLSGWVNVWRAWLQNKSAPSFWQPYRMLHKLLNKESVVADNASPLFRVTPYVVFGCMTLA
CSIIPTLSTDLPLAPAADAIALVSLFALARVFISLAAMDVGTAFGTMGARREMLIGFLAEPALLMVLFSASLISHSTSLA
TIVETLAHRELTIYPGMAFAGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEYSGRHLALLEWAASLKLFAYSCIG
LALFIPWGVGEANAPLSVLLALPALMLKLLLGGFLLALLETVNAKMRIFRVPEFLATAFLLAVIGLLVHLLLVH

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=298, Percent_Identity=34.2281879194631, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI87082115, Length=250, Percent_Identity=33.2, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 33970; Mature: 33839

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATGVLAQLLEIVISIVLAPLLSGWVNVWRAWLQNKSAPSFWQPYRMLHKLLNKESVVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEC
DNASPLFRVTPYVVFGCMTLACSIIPTLSTDLPLAPAADAIALVSLFALARVFISLAAMD
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGTAFGTMGARREMLIGFLAEPALLMVLFSASLISHSTSLATIVETLAHRELTIYPGMAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHH
AGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEYSGRHLALLEWAASLKLFAYSCI
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GLALFIPWGVGEANAPLSVLLALPALMLKLLLGGFLLALLETVNAKMRIFRVPEFLATAF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEECHHHHHHHH
LLAVIGLLVHLLLVH
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATGVLAQLLEIVISIVLAPLLSGWVNVWRAWLQNKSAPSFWQPYRMLHKLLNKESVVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEC
DNASPLFRVTPYVVFGCMTLACSIIPTLSTDLPLAPAADAIALVSLFALARVFISLAAMD
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGTAFGTMGARREMLIGFLAEPALLMVLFSASLISHSTSLATIVETLAHRELTIYPGMAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHH
AGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEYSGRHLALLEWAASLKLFAYSCI
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GLALFIPWGVGEANAPLSVLLALPALMLKLLLGGFLLALLETVNAKMRIFRVPEFLATAF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEECHHHHHHHH
LLAVIGLLVHLLLVH
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]