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The map label for this gene is hyfB [H]

Identifier: 94313593

GI number: 94313593

Start: 1303145

End: 1305169

Strand: Reverse

Name: hyfB [H]

Synonym: Rmet_4671

Alternate gene names: 94313593

Gene position: 1305169-1303145 (Counterclockwise)

Preceding gene: 94313594

Following gene: 94313592

Centisome position: 50.59

GC content: 63.21

Gene sequence:

>2025_bases
GTGGACGGCGTGCCTGCGGTCGGACAGTGGTTGTTGCTCGACTGGATGCTCGTTGTCACGGCCGCGTGGCTGATGATCGG
GGCAACCGGTGTGCTCGCACTGCATCGTCTGGCACTGGTGTCGCGCCTGCTCTTCCCTCTGGGCGCTCTGCTCGGGCTGG
TGCTCAGCGCGCTGGCTCTTGCGGCGGTGGGTAACAGCCCCGAAGTGGCAGTGCTTTCCATCGGGCTGCCGCGTCTGCCT
TTCCATCTCCGGCTGGATAGCCTGTCCGCCTATTTCCTGATGGTAATCGGTGCCGCAGCGGCCGGAATCTCCGTGTTCGC
GGCGGGTTACTTCCGCCAAGGCGAGGGCACGCCCCCCGGCTTGCTATGCCTGGAGTATCACCTGTTCCTGGCCAGCATGG
CTGGCGTTGTGCTGGCCGACGACGCCTACGCGTTCATGGTGATGTGGGAGACCATGGCGTTGTCATCGTTCTTCCTGGCG
ACCGCCAACCACCGCATCGCGGAGGTTCGCAGCGCCGGCTACCTGTACATCACCATGGCACACATCGGGGCGATCGCGGT
CCTGTTGTGCTTTGGCGTCCTGCAGGCCAATACCGGCGACTACACCTTCGCGAACATGCGCGCCCAAACGCTGACGCCGT
TCTGGGCGTCGGTCGCGTTCCTGCTTGCAATGTTCGGCTTCGGCGCCAAGGCCGGTCTGCTTCCACTGCATGTGTGGCTG
CCCGAAGCGCACCCGGCTGCGCCATCGCCGGTGTCGGCAATGATGAGTGGCGTCATGCTCAACACCGCCATTTATGGCCT
CTTGCGTGTGGTTCTGGACCTGGCACAGATACATCTGTGGTGGTGGGGCGGGCTACTGTTGGCCTCCGGCCTGGCCACCG
CGCTGTATGGCGTGGTGTTCGCCGCAGTGCAAACCGACATGAAGCGGTTGCTGGCCTACTCATCGATTGAGAACATGGGC
CTGCTGTTCGTTGCTATTGGCCTCGCTTTGATCTTCTCGGCCTACGGCATGAAACCGTTGGCCGCTCTGGCACTGACCGC
AGCGCTATATCACGTGGCCAGCCATGCCTTCTTCAAGAGCCTGCTTTTCCTTGGTACTGGCAGCGTGCTGCATGCTACTG
CCGAGCGCAGCCTCGGCAAGCTGGGAGGTTTGATACGCCAGATGCCGTGGGTGGGCTGGCTCACGCTGGTGGGCGTGCTG
ACCAGCGCGGGTCTGCCACCGCTTGGTGGGTTCGTCTCGGAGTGGTTGCTCCTGCAGAGCTTTCTGTTTACGACCGGATT
GCCGGTGCCGCTCCTGACCATGCTTATCCCCGTGGTGGCGGCGCTGATTGCGCTGGTAGCGGCCCTGGCCGGCTACACGA
TGGTGAAGTTCTTCGGTGTGATCTTTCTCGGCCTTCCGCGCGAGCCCAGACTCAACGACGCGCAGGACGCTGGCGGCTGG
GAGCGCGCCGGCATGCTATGGCTGGCACTGGGAAGCATCGCGCTTGGACTATTGCCATCTCCTTTCATCGGCCTGATCGA
TGCAGTGACGCAGCAATTGGTCGGCAGCGGCCTCGGCGCACGTGTCGCGACCAGCGGCTGGCTGCTTGCGCCGATCAGTC
CGGAGCAGGCCAGCTACGGGCCGGTCATCTTCCTGCTAGGTATCCTTGCCAGTTTCGCGCTTGCCTATCTTCTTGTGCGC
GGTCTCTACCATGGCAGGCTGCGTCGCAGCGTGGCCTGGGCCTGCGGCTTTCCGTGGGGCACCCCGCGCATGCAAGATAC
CGCCGAGGGTTTCGGCCAGCCGATTCGGCAGATCTTCGAGCCCTTTTTTCAGATTAGGCGTGACCTGCCCACCCCGTTCG
ACGAACACCCGCAATATCGGGTGATAGTGTCAGATCCGTTCTGGCGCTGGCTGTACTTGCCCGTGGTCCGCCTGGTCGCG
GTTATGGCACGCGCGGTCGGTGCAATTCAGCAGGGGCGCATTTCAGCGTATCTGCTGTTCAGCTTTCTGACGTTGATTGC
CGTGCTGCTGGCGGTCATGCGGTGA

Upstream 100 bases:

>100_bases
CAAACCGGCCACCGCTAAAAAGCCTGCCAGGAAGAAGTCATCGCAGCGCGATACGACTTCGCCCGCCAGGCGGCGCACGG
TTCGCCGCGGAGCCTGAGTC

Downstream 100 bases:

>100_bases
GGCCATACCCATGAGCGCAACCGGCGTGCTGGCACAACTGCTCGAGATAGTGATCTCGATTGTCCTGGCGCCGTTGCTTT
CCGGCTGGGTCAATGTGTGG

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MDGVPAVGQWLLLDWMLVVTAAWLMIGATGVLALHRLALVSRLLFPLGALLGLVLSALALAAVGNSPEVAVLSIGLPRLP
FHLRLDSLSAYFLMVIGAAAAGISVFAAGYFRQGEGTPPGLLCLEYHLFLASMAGVVLADDAYAFMVMWETMALSSFFLA
TANHRIAEVRSAGYLYITMAHIGAIAVLLCFGVLQANTGDYTFANMRAQTLTPFWASVAFLLAMFGFGAKAGLLPLHVWL
PEAHPAAPSPVSAMMSGVMLNTAIYGLLRVVLDLAQIHLWWWGGLLLASGLATALYGVVFAAVQTDMKRLLAYSSIENMG
LLFVAIGLALIFSAYGMKPLAALALTAALYHVASHAFFKSLLFLGTGSVLHATAERSLGKLGGLIRQMPWVGWLTLVGVL
TSAGLPPLGGFVSEWLLLQSFLFTTGLPVPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGLPREPRLNDAQDAGGW
ERAGMLWLALGSIALGLLPSPFIGLIDAVTQQLVGSGLGARVATSGWLLAPISPEQASYGPVIFLLGILASFALAYLLVR
GLYHGRLRRSVAWACGFPWGTPRMQDTAEGFGQPIRQIFEPFFQIRRDLPTPFDEHPQYRVIVSDPFWRWLYLPVVRLVA
VMARAVGAIQQGRISAYLLFSFLTLIAVLLAVMR

Sequences:

>Translated_674_residues
MDGVPAVGQWLLLDWMLVVTAAWLMIGATGVLALHRLALVSRLLFPLGALLGLVLSALALAAVGNSPEVAVLSIGLPRLP
FHLRLDSLSAYFLMVIGAAAAGISVFAAGYFRQGEGTPPGLLCLEYHLFLASMAGVVLADDAYAFMVMWETMALSSFFLA
TANHRIAEVRSAGYLYITMAHIGAIAVLLCFGVLQANTGDYTFANMRAQTLTPFWASVAFLLAMFGFGAKAGLLPLHVWL
PEAHPAAPSPVSAMMSGVMLNTAIYGLLRVVLDLAQIHLWWWGGLLLASGLATALYGVVFAAVQTDMKRLLAYSSIENMG
LLFVAIGLALIFSAYGMKPLAALALTAALYHVASHAFFKSLLFLGTGSVLHATAERSLGKLGGLIRQMPWVGWLTLVGVL
TSAGLPPLGGFVSEWLLLQSFLFTTGLPVPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGLPREPRLNDAQDAGGW
ERAGMLWLALGSIALGLLPSPFIGLIDAVTQQLVGSGLGARVATSGWLLAPISPEQASYGPVIFLLGILASFALAYLLVR
GLYHGRLRRSVAWACGFPWGTPRMQDTAEGFGQPIRQIFEPFFQIRRDLPTPFDEHPQYRVIVSDPFWRWLYLPVVRLVA
VMARAVGAIQQGRISAYLLFSFLTLIAVLLAVMR
>Mature_674_residues
MDGVPAVGQWLLLDWMLVVTAAWLMIGATGVLALHRLALVSRLLFPLGALLGLVLSALALAAVGNSPEVAVLSIGLPRLP
FHLRLDSLSAYFLMVIGAAAAGISVFAAGYFRQGEGTPPGLLCLEYHLFLASMAGVVLADDAYAFMVMWETMALSSFFLA
TANHRIAEVRSAGYLYITMAHIGAIAVLLCFGVLQANTGDYTFANMRAQTLTPFWASVAFLLAMFGFGAKAGLLPLHVWL
PEAHPAAPSPVSAMMSGVMLNTAIYGLLRVVLDLAQIHLWWWGGLLLASGLATALYGVVFAAVQTDMKRLLAYSSIENMG
LLFVAIGLALIFSAYGMKPLAALALTAALYHVASHAFFKSLLFLGTGSVLHATAERSLGKLGGLIRQMPWVGWLTLVGVL
TSAGLPPLGGFVSEWLLLQSFLFTTGLPVPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGLPREPRLNDAQDAGGW
ERAGMLWLALGSIALGLLPSPFIGLIDAVTQQLVGSGLGARVATSGWLLAPISPEQASYGPVIFLLGILASFALAYLLVR
GLYHGRLRRSVAWACGFPWGTPRMQDTAEGFGQPIRQIFEPFFQIRRDLPTPFDEHPQYRVIVSDPFWRWLYLPVVRLVA
VMARAVGAIQQGRISAYLLFSFLTLIAVLLAVMR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=299, Percent_Identity=30.4347826086957, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788827, Length=599, Percent_Identity=34.5575959933222, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI2367154, Length=578, Percent_Identity=29.757785467128, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1788831, Length=363, Percent_Identity=34.435261707989, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1788613, Length=381, Percent_Identity=29.9212598425197, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788829, Length=398, Percent_Identity=28.894472361809, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1788614, Length=363, Percent_Identity=31.404958677686, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI145693160, Length=402, Percent_Identity=25.6218905472637, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 72354; Mature: 72354

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVPAVGQWLLLDWMLVVTAAWLMIGATGVLALHRLALVSRLLFPLGALLGLVLSALAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVGNSPEVAVLSIGLPRLPFHLRLDSLSAYFLMVIGAAAAGISVFAAGYFRQGEGTPPG
HHCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
LLCLEYHLFLASMAGVVLADDAYAFMVMWETMALSSFFLATANHRIAEVRSAGYLYITMA
HHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEHHH
HIGAIAVLLCFGVLQANTGDYTFANMRAQTLTPFWASVAFLLAMFGFGAKAGLLPLHVWL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PEAHPAAPSPVSAMMSGVMLNTAIYGLLRVVLDLAQIHLWWWGGLLLASGLATALYGVVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVQTDMKRLLAYSSIENMGLLFVAIGLALIFSAYGMKPLAALALTAALYHVASHAFFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLFLGTGSVLHATAERSLGKLGGLIRQMPWVGWLTLVGVLTSAGLPPLGGFVSEWLLLQS
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLFTTGLPVPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGLPREPRLNDAQDAGGW
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
ERAGMLWLALGSIALGLLPSPFIGLIDAVTQQLVGSGLGARVATSGWLLAPISPEQASYG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCCCCCCC
PVIFLLGILASFALAYLLVRGLYHGRLRRSVAWACGFPWGTPRMQDTAEGFGQPIRQIFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
PFFQIRRDLPTPFDEHPQYRVIVSDPFWRWLYLPVVRLVAVMARAVGAIQQGRISAYLLF
HHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SFLTLIAVLLAVMR
HHHHHHHHHHHHHC
>Mature Secondary Structure
MDGVPAVGQWLLLDWMLVVTAAWLMIGATGVLALHRLALVSRLLFPLGALLGLVLSALAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVGNSPEVAVLSIGLPRLPFHLRLDSLSAYFLMVIGAAAAGISVFAAGYFRQGEGTPPG
HHCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
LLCLEYHLFLASMAGVVLADDAYAFMVMWETMALSSFFLATANHRIAEVRSAGYLYITMA
HHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEHHH
HIGAIAVLLCFGVLQANTGDYTFANMRAQTLTPFWASVAFLLAMFGFGAKAGLLPLHVWL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PEAHPAAPSPVSAMMSGVMLNTAIYGLLRVVLDLAQIHLWWWGGLLLASGLATALYGVVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVQTDMKRLLAYSSIENMGLLFVAIGLALIFSAYGMKPLAALALTAALYHVASHAFFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLFLGTGSVLHATAERSLGKLGGLIRQMPWVGWLTLVGVLTSAGLPPLGGFVSEWLLLQS
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLFTTGLPVPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGLPREPRLNDAQDAGGW
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
ERAGMLWLALGSIALGLLPSPFIGLIDAVTQQLVGSGLGARVATSGWLLAPISPEQASYG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCCCCCCC
PVIFLLGILASFALAYLLVRGLYHGRLRRSVAWACGFPWGTPRMQDTAEGFGQPIRQIFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
PFFQIRRDLPTPFDEHPQYRVIVSDPFWRWLYLPVVRLVAVMARAVGAIQQGRISAYLLF
HHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SFLTLIAVLLAVMR
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]