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The map label for this gene is gacA [H]

Identifier: 94313441

GI number: 94313441

Start: 1121351

End: 1121995

Strand: Direct

Name: gacA [H]

Synonym: Rmet_4516

Alternate gene names: 94313441

Gene position: 1121351-1121995 (Clockwise)

Preceding gene: 94313440

Following gene: 94313443

Centisome position: 43.46

GC content: 64.5

Gene sequence:

>645_bases
ATGATTCGCGTGATCATTGCAGACGATCACACTGTGGTGCGCAACGGATTGCGCCATATCCTCGAACGCACCAGCGACTT
CGAGGTGGTTGGCGAAGCAGCCCGCGGTACCGAAGTGCCACAACTCGTGCGCGAGCTGTCGCCGCATGTCATCCTGCTGG
ACCTGTCGATGCCCGGCCGGAACGGGCTGGAACTGATCCGGCAGCTGCGGACCGATCATCCGGCACTACGAATCCTCGTG
CTGACGATGCACGCGGAGGAACAGTATGTGGTCCGCGCCTTCCGTGCCGGCGCGGCTGGCTACCTGACCAAGGAAAGCGC
GGCGACAGAACTGGTGGAGGCGCTGAGGAAGGTGGCCGCGGGCGGCACCTACGTCAGCCCCGCCATGGCCGAAAAGCTGG
CGCTGGGCCTGCAGGAAAAGCCCGATGCGCCGCACGAGGGCCTGTCGGATCGTGAACTCGAGGTCTTCCGGCGCATCGTC
GACGGCGAGTCGCTCACGCAGATCGCCGCCGCGCTGTGCGTCAGCGCCAAGACGGTCAGCACCTACAAGATGCGCCTGAT
GGAAAAACTCCAGCTTCGCAGCGACGCCGCGCTGGTCCGCTATGCGATCGAGGCGAGGCTGTTCGGCGACGAAGACAAGC
TATAG

Upstream 100 bases:

>100_bases
GCCTGCTGGGTGGCACGGTGTCCATCGAACATTCGCCCGGCGAGGGCTTCGAACTCGTCGTCACGTTTCCGGGTCATCCG
GATTGCAAGGAGACCCAGGC

Downstream 100 bases:

>100_bases
CGTCGTGACCCCCAGCCACAGCCCCGTCGTTCAGGCCGCCTCGCCGCGAATCAGCAGCACCGGCTTGCTGGTCTTGGCCA
CCACACCTTCAGAGACGCTA

Product: LuxR family two component transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MIRVIIADDHTVVRNGLRHILERTSDFEVVGEAARGTEVPQLVRELSPHVILLDLSMPGRNGLELIRQLRTDHPALRILV
LTMHAEEQYVVRAFRAGAAGYLTKESAATELVEALRKVAAGGTYVSPAMAEKLALGLQEKPDAPHEGLSDRELEVFRRIV
DGESLTQIAAALCVSAKTVSTYKMRLMEKLQLRSDAALVRYAIEARLFGDEDKL

Sequences:

>Translated_214_residues
MIRVIIADDHTVVRNGLRHILERTSDFEVVGEAARGTEVPQLVRELSPHVILLDLSMPGRNGLELIRQLRTDHPALRILV
LTMHAEEQYVVRAFRAGAAGYLTKESAATELVEALRKVAAGGTYVSPAMAEKLALGLQEKPDAPHEGLSDRELEVFRRIV
DGESLTQIAAALCVSAKTVSTYKMRLMEKLQLRSDAALVRYAIEARLFGDEDKL
>Mature_214_residues
MIRVIIADDHTVVRNGLRHILERTSDFEVVGEAARGTEVPQLVRELSPHVILLDLSMPGRNGLELIRQLRTDHPALRILV
LTMHAEEQYVVRAFRAGAAGYLTKESAATELVEALRKVAAGGTYVSPAMAEKLALGLQEKPDAPHEGLSDRELEVFRRIV
DGESLTQIAAALCVSAKTVSTYKMRLMEKLQLRSDAALVRYAIEARLFGDEDKL

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=213, Percent_Identity=38.0281690140845, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=36.5, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1788521, Length=200, Percent_Identity=37, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=31.3725490196078, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788546, Length=206, Percent_Identity=34.9514563106796, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786747, Length=199, Percent_Identity=26.6331658291457, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=34.6153846153846, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=37.5, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=30.2521008403361, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23680; Mature: 23680

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVIIADDHTVVRNGLRHILERTSDFEVVGEAARGTEVPQLVRELSPHVILLDLSMPGR
CEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCC
NGLELIRQLRTDHPALRILVLTMHAEEQYVVRAFRAGAAGYLTKESAATELVEALRKVAA
HHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GGTYVSPAMAEKLALGLQEKPDAPHEGLSDRELEVFRRIVDGESLTQIAAALCVSAKTVS
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TYKMRLMEKLQLRSDAALVRYAIEARLFGDEDKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIRVIIADDHTVVRNGLRHILERTSDFEVVGEAARGTEVPQLVRELSPHVILLDLSMPGR
CEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCC
NGLELIRQLRTDHPALRILVLTMHAEEQYVVRAFRAGAAGYLTKESAATELVEALRKVAA
HHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GGTYVSPAMAEKLALGLQEKPDAPHEGLSDRELEVFRRIVDGESLTQIAAALCVSAKTVS
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TYKMRLMEKLQLRSDAALVRYAIEARLFGDEDKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]