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The map label for this gene is gacS [H]

Identifier: 94313180

GI number: 94313180

Start: 841982

End: 844027

Strand: Direct

Name: gacS [H]

Synonym: Rmet_4254

Alternate gene names: 94313180

Gene position: 841982-844027 (Clockwise)

Preceding gene: 94313179

Following gene: 94313181

Centisome position: 32.63

GC content: 66.28

Gene sequence:

>2046_bases
ATGCGCATCCAACACGCATTCCGCCTGATCACGCTTGCCATGGTGGCGACGATCGTGGGCTTCGGCAGCTATCTCTGGAC
CGAGGAGTGGCGGGCATACGCCGTGGCGCAACACAGCGAGGCGATGCTCCAGGCCTTTCGCGCCACGCTGGTTGCCGCCG
AGAAGGTCTCGGCCGAGCGGGGGCCATCCAATGCCGCGTTGGGCGCAGCGCCTGGCGTCGCCGCCGGCGCGCTGTTGCGA
GCGCGGGAACAGAGTGACGCCAGTCTGACTGCGGCCATAGCAATGTTGCACGGTGCCAACTGCCCGGAGTGCGGCGCGGC
CAAAGGCAGCCTCGAGCATGCCCGGGCGCAGTTGGCGGCAGCGCGGTCCCAGATCGACCGGCTAATCGCCCGGCCATTGT
CGGAACGACATGATGTGCAACGGGCCGTGAGCGGGATGTTCACCATCGTCGATGAGATCCTGCGCGTCGCGGACGGCCCG
CTGTCGCGCCTGCAGCGCTCCGCTCCTGTGGCGGCGCAGCACGCGGTCAGTGCGCGATTTGCCGCCGAACTGCGCGAGTA
TGCGGGTCGCGCCGGCTCCGAGTTCACCAGCGCACTGGTCAGCAATCGTCCGATGCACAGCGAGGAGCGCGAGCGCCTGG
CGGGGGATCTCGGGCGCGTGCAGACGTTGGGGGACCAGATCCAGCAGCGCGTCAGCTTCCAGTTGGCGTCGAAACCCGGG
CTACGCGACGCCGACACTGCGCTGCACAAGCGCTTTTTCGGTGACGGCCTCGCCTATCTCCAGAAGGTCGAGGCGATCGG
TCCGGCGGAACCTCGGCCGACGACGGCAGAGTTGGCCAGCACCTACGTGCCACTGATGGCGCCGATCGTCGCCATGCGCG
ACGCCGTGCTCGACGTGGCTCAGGCGGAAGTGCAGGCTGACACGACGCGCGCGAAGAGAAGGCTTGGTGGCTTGTTGGCG
ATCCTCGCGATCATCACGTCGTTTGTCGCAGCCACGTTCTGGCTGTTCGCGAAGCGGGTGCTGCGTCCACTTACGCTAGC
GACGGAGGCCACCGTTGAGCTGGCCGAGGGGCGATACGACGGGCCGACACATGTCTGGCCCAAGACCGACGAGATTGGGG
CCATCTTTGTCGCGCTGGAACAGTTGCGGGCGGGCCTGCTGCGGAAGGTGGCGCTCGAGCATGAGCGCACGACGCTGATC
GCGAGGCTGCAGGCGGCAGTCACGCACGAGCGTGCCCAGATGATGGCGCTGGCCCAGGCGCGCGACGCAGCCGAGGCCTC
GGCGCGCACCAAGAGCAGCTTCCTGGCCATGATGAGCCACGAGATCCGCACGCCGTTGCAGGGCATCCTCGGCCTGCTGG
AACTGGTGGAGTATTCGCCTTTGTCAGCCGAACAGCGAAGGCAAGTGCGTCTGGCCTCGGAGGCAGGTCAGGCGCTGCGA
CAGATCCTCGACGATGTGCTCGACTACGCCAAGATGGACGCGGGCCGGCTGCGTCTTGCGTTAGCGCCACTTGATTTGCG
CGGGCTGTTCGCCAATGTGCTGAGCCTATTGGCGCCGCGCGCGCAGGCCAAAGGGTTGCAGTTGCAGCAATGGGTGCAGC
CCGACGTTCCGGTTCAACTCGTGGCCGACGGGGCTCGGCTGCGACAGATTCTTCTCAACCTCGTGGGCAACGCGATCAAG
TTCACCGAGTGCGGCAGCGTGGATTTGCTCGCCAGTGTTGAGCCGACCAGCGCCGGCGAGGACGTGCTGGTGGTCTCGGT
GGCGGACACAGGCATCGGGATCGCACCGGAGGATATTGCACGGCTCTTTACCCCATTCGTGCAGGGCGAGAGCGGACCGG
CGCGCCGTTTCGACGGAACCGGACTGGGCCTGGCAATTTCTCGGCAACTGGCGCAACTGATGGGAGGTGAGCTGATGCTC
GACAGTACACTAGGTGTTGGTACGCAGGTGATGCTGCGCGTGCCGATGCCGGGTTGCCCCGACGCTGGATCGCCCCTGTC
GAACTCTGAGAGACGAGAGCATCGCGCCGAGTTCAGGTTGGCATAG

Upstream 100 bases:

>100_bases
CCCGCCGCCAGCCAACATAACCGGGTCGATCACCGTCGACCGTCTCGTAAGAGGCACGCGTGGCACACACAAGCGACAAT
CCTGAGAGCACGCTTCATCC

Downstream 100 bases:

>100_bases
GTGGCGGCATCAAGTTGATGCGCATGATCGCCAAGGATCTGAGGCGGGACCTCGAAAGAAGAAATCCGCCCGCTAAAGAA
TTGCCTGCGCCATTTTTTTC

Product: periplasmic sensor signal transduction histidine kinase; putative exported protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MRIQHAFRLITLAMVATIVGFGSYLWTEEWRAYAVAQHSEAMLQAFRATLVAAEKVSAERGPSNAALGAAPGVAAGALLR
AREQSDASLTAAIAMLHGANCPECGAAKGSLEHARAQLAAARSQIDRLIARPLSERHDVQRAVSGMFTIVDEILRVADGP
LSRLQRSAPVAAQHAVSARFAAELREYAGRAGSEFTSALVSNRPMHSEERERLAGDLGRVQTLGDQIQQRVSFQLASKPG
LRDADTALHKRFFGDGLAYLQKVEAIGPAEPRPTTAELASTYVPLMAPIVAMRDAVLDVAQAEVQADTTRAKRRLGGLLA
ILAIITSFVAATFWLFAKRVLRPLTLATEATVELAEGRYDGPTHVWPKTDEIGAIFVALEQLRAGLLRKVALEHERTTLI
ARLQAAVTHERAQMMALAQARDAAEASARTKSSFLAMMSHEIRTPLQGILGLLELVEYSPLSAEQRRQVRLASEAGQALR
QILDDVLDYAKMDAGRLRLALAPLDLRGLFANVLSLLAPRAQAKGLQLQQWVQPDVPVQLVADGARLRQILLNLVGNAIK
FTECGSVDLLASVEPTSAGEDVLVVSVADTGIGIAPEDIARLFTPFVQGESGPARRFDGTGLGLAISRQLAQLMGGELML
DSTLGVGTQVMLRVPMPGCPDAGSPLSNSERREHRAEFRLA

Sequences:

>Translated_681_residues
MRIQHAFRLITLAMVATIVGFGSYLWTEEWRAYAVAQHSEAMLQAFRATLVAAEKVSAERGPSNAALGAAPGVAAGALLR
AREQSDASLTAAIAMLHGANCPECGAAKGSLEHARAQLAAARSQIDRLIARPLSERHDVQRAVSGMFTIVDEILRVADGP
LSRLQRSAPVAAQHAVSARFAAELREYAGRAGSEFTSALVSNRPMHSEERERLAGDLGRVQTLGDQIQQRVSFQLASKPG
LRDADTALHKRFFGDGLAYLQKVEAIGPAEPRPTTAELASTYVPLMAPIVAMRDAVLDVAQAEVQADTTRAKRRLGGLLA
ILAIITSFVAATFWLFAKRVLRPLTLATEATVELAEGRYDGPTHVWPKTDEIGAIFVALEQLRAGLLRKVALEHERTTLI
ARLQAAVTHERAQMMALAQARDAAEASARTKSSFLAMMSHEIRTPLQGILGLLELVEYSPLSAEQRRQVRLASEAGQALR
QILDDVLDYAKMDAGRLRLALAPLDLRGLFANVLSLLAPRAQAKGLQLQQWVQPDVPVQLVADGARLRQILLNLVGNAIK
FTECGSVDLLASVEPTSAGEDVLVVSVADTGIGIAPEDIARLFTPFVQGESGPARRFDGTGLGLAISRQLAQLMGGELML
DSTLGVGTQVMLRVPMPGCPDAGSPLSNSERREHRAEFRLA
>Mature_681_residues
MRIQHAFRLITLAMVATIVGFGSYLWTEEWRAYAVAQHSEAMLQAFRATLVAAEKVSAERGPSNAALGAAPGVAAGALLR
AREQSDASLTAAIAMLHGANCPECGAAKGSLEHARAQLAAARSQIDRLIARPLSERHDVQRAVSGMFTIVDEILRVADGP
LSRLQRSAPVAAQHAVSARFAAELREYAGRAGSEFTSALVSNRPMHSEERERLAGDLGRVQTLGDQIQQRVSFQLASKPG
LRDADTALHKRFFGDGLAYLQKVEAIGPAEPRPTTAELASTYVPLMAPIVAMRDAVLDVAQAEVQADTTRAKRRLGGLLA
ILAIITSFVAATFWLFAKRVLRPLTLATEATVELAEGRYDGPTHVWPKTDEIGAIFVALEQLRAGLLRKVALEHERTTLI
ARLQAAVTHERAQMMALAQARDAAEASARTKSSFLAMMSHEIRTPLQGILGLLELVEYSPLSAEQRRQVRLASEAGQALR
QILDDVLDYAKMDAGRLRLALAPLDLRGLFANVLSLLAPRAQAKGLQLQQWVQPDVPVQLVADGARLRQILLNLVGNAIK
FTECGSVDLLASVEPTSAGEDVLVVSVADTGIGIAPEDIARLFTPFVQGESGPARRFDGTGLGLAISRQLAQLMGGELML
DSTLGVGTQVMLRVPMPGCPDAGSPLSNSERREHRAEFRLA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=270, Percent_Identity=37.4074074074074, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081816, Length=350, Percent_Identity=36.5714285714286, Blast_Score=182, Evalue=9e-47,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=38.2716049382716, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=36.3636363636364, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788713, Length=253, Percent_Identity=33.596837944664, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790436, Length=304, Percent_Identity=28.6184210526316, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=233, Percent_Identity=27.4678111587983, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1786600, Length=252, Percent_Identity=28.1746031746032, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082128, Length=240, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786912, Length=260, Percent_Identity=28.8461538461538, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=26.5486725663717, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=28.3950617283951, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790861, Length=247, Percent_Identity=26.7206477732794, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=75, Percent_Identity=45.3333333333333, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73253; Mature: 73253

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQHAFRLITLAMVATIVGFGSYLWTEEWRAYAVAQHSEAMLQAFRATLVAAEKVSAER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPSNAALGAAPGVAAGALLRAREQSDASLTAAIAMLHGANCPECGAAKGSLEHARAQLAA
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ARSQIDRLIARPLSERHDVQRAVSGMFTIVDEILRVADGPLSRLQRSAPVAAQHAVSARF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHH
AAELREYAGRAGSEFTSALVSNRPMHSEERERLAGDLGRVQTLGDQIQQRVSFQLASKPG
HHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LRDADTALHKRFFGDGLAYLQKVEAIGPAEPRPTTAELASTYVPLMAPIVAMRDAVLDVA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEVQADTTRAKRRLGGLLAILAIITSFVAATFWLFAKRVLRPLTLATEATVELAEGRYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPTHVWPKTDEIGAIFVALEQLRAGLLRKVALEHERTTLIARLQAAVTHERAQMMALAQA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAAEASARTKSSFLAMMSHEIRTPLQGILGLLELVEYSPLSAEQRRQVRLASEAGQALR
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QILDDVLDYAKMDAGRLRLALAPLDLRGLFANVLSLLAPRAQAKGLQLQQWVQPDVPVQL
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCCEE
VADGARLRQILLNLVGNAIKFTECGSVDLLASVEPTSAGEDVLVVSVADTGIGIAPEDIA
CCCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHH
RLFTPFVQGESGPARRFDGTGLGLAISRQLAQLMGGELMLDSTLGVGTQVMLRVPMPGCP
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCC
DAGSPLSNSERREHRAEFRLA
CCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRIQHAFRLITLAMVATIVGFGSYLWTEEWRAYAVAQHSEAMLQAFRATLVAAEKVSAER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPSNAALGAAPGVAAGALLRAREQSDASLTAAIAMLHGANCPECGAAKGSLEHARAQLAA
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ARSQIDRLIARPLSERHDVQRAVSGMFTIVDEILRVADGPLSRLQRSAPVAAQHAVSARF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHH
AAELREYAGRAGSEFTSALVSNRPMHSEERERLAGDLGRVQTLGDQIQQRVSFQLASKPG
HHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LRDADTALHKRFFGDGLAYLQKVEAIGPAEPRPTTAELASTYVPLMAPIVAMRDAVLDVA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEVQADTTRAKRRLGGLLAILAIITSFVAATFWLFAKRVLRPLTLATEATVELAEGRYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPTHVWPKTDEIGAIFVALEQLRAGLLRKVALEHERTTLIARLQAAVTHERAQMMALAQA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAAEASARTKSSFLAMMSHEIRTPLQGILGLLELVEYSPLSAEQRRQVRLASEAGQALR
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QILDDVLDYAKMDAGRLRLALAPLDLRGLFANVLSLLAPRAQAKGLQLQQWVQPDVPVQL
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCCEE
VADGARLRQILLNLVGNAIKFTECGSVDLLASVEPTSAGEDVLVVSVADTGIGIAPEDIA
CCCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHH
RLFTPFVQGESGPARRFDGTGLGLAISRQLAQLMGGELMLDSTLGVGTQVMLRVPMPGCP
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCC
DAGSPLSNSERREHRAEFRLA
CCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]