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The map label for this gene is pdhC [H]

Identifier: 94313059

GI number: 94313059

Start: 706284

End: 707417

Strand: Reverse

Name: pdhC [H]

Synonym: Rmet_4132

Alternate gene names: 94313059

Gene position: 707417-706284 (Counterclockwise)

Preceding gene: 94313060

Following gene: 94313058

Centisome position: 27.42

GC content: 70.11

Gene sequence:

>1134_bases
ATGCGGATCTTCAAGCTGCCCGACCTGGGCGAAGGCCTGCAGGAAGCGGAAATCGTCAACTGGCATGTGAAGCCCGGGGA
CACCATCGCAGCCGACCAGCCGCTGTTGTCGGTGGAAACAGCCAAGGCCATCGTCGAGATCCCCTCCCCGTTCGCCGGAC
AGGTTGCGAAACTCTTTGCGCAACCGGGCGACATCGTCCACCTGGGCGCGCCGCTGGTTGGCTTCGAGGGCGCGGGGGCG
TCCGACGATGCCGGCACGGTGGTAGGCGCCGTGAAGGTCGGCACGCATGTGGTCAATGAAGGCGTCACGCGCGTAGGCGC
CGCCGGCATTCCCGGCGGACATGGCATCAAGGCCACGCCGGCCGTGCGCGCGCTGGCGCGCAAGCTGTCGGTCGACCTGT
CGATGGTCACCCCATCCGGCCACGATGGCGTGATCACGGCCACCGATGTGCAGCGCGTGGCCACCACCCTGGCGGACGTC
GGCGCGCCGGACGTGCTGCGCGGTGTGCGCCGCGCAATGGCGCAGAACATGGCGCGCGCGCAGAGCGAAGTGGCCGCGGC
CACGGTCATGGACGATGCGGACCTGCATGCGTGGCAGGCGACCGGCGCATCGGTGACCGGTCAGGACATCACGATCCGCC
TGGTGCGCGCGCTGGTGGCCGGCGTGCGCGCGGAACCGGCGCTCAACGCGTGGTACGAAGGCAGGACCGGCAGACGCCAC
CTGCTCGAGCGGATCGACGTGGGCATTGCGGCGGACCTGCCGGAGGGGTTGTTCGTGCCGGTGCTGCGCAACGTCGGCAA
ACGCGATGCCGCCGACCTGCGTGCCGGGCTGGACCGAATGCGCGCCGACGTGGTCGCACGCACCATCGCGCCGGAGGAGA
TGCGCGGCAATACCATCACCCTGTCGAACTTCGGCATGATCGCGGGCCGCTACGCGGCGCCCATCGTGGTGCCGCCAACG
GTTGCCATCCTCGGCGCGGGGCGCGTGCGCGACGAAGTGGTGGCATCCGGCGGCGTGCCTGCCGTGCACCGGGTCATGCC
ACTGTCGCTGACATTCGATCACCGCGTGGTCACGGGCGGCGAGGCGGCCCGGTTCCTGCGCGCGGTCATCGAGGATCTGG
AACGGCCCGAGTAG

Upstream 100 bases:

>100_bases
CCGCTGGCGCGACTCGAACACAGCTACATGCCGTCGGTGGCGCGGATTGTCGACGCGGCGCGACGCGCGCTGGAAGCAGC
CTAGCCAAGGAGAGATAGCG

Downstream 100 bases:

>100_bases
CACGACAGGAGTGATTCCGGCGATACCGGCACGGCCCTGGCCAGGCCGGCCATCCGCCACATGGATGAAGCCCATTGTCC
GTCCGGCGGGCAAACCAGTT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MRIFKLPDLGEGLQEAEIVNWHVKPGDTIAADQPLLSVETAKAIVEIPSPFAGQVAKLFAQPGDIVHLGAPLVGFEGAGA
SDDAGTVVGAVKVGTHVVNEGVTRVGAAGIPGGHGIKATPAVRALARKLSVDLSMVTPSGHDGVITATDVQRVATTLADV
GAPDVLRGVRRAMAQNMARAQSEVAAATVMDDADLHAWQATGASVTGQDITIRLVRALVAGVRAEPALNAWYEGRTGRRH
LLERIDVGIAADLPEGLFVPVLRNVGKRDAADLRAGLDRMRADVVARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPT
VAILGAGRVRDEVVASGGVPAVHRVMPLSLTFDHRVVTGGEAARFLRAVIEDLERPE

Sequences:

>Translated_377_residues
MRIFKLPDLGEGLQEAEIVNWHVKPGDTIAADQPLLSVETAKAIVEIPSPFAGQVAKLFAQPGDIVHLGAPLVGFEGAGA
SDDAGTVVGAVKVGTHVVNEGVTRVGAAGIPGGHGIKATPAVRALARKLSVDLSMVTPSGHDGVITATDVQRVATTLADV
GAPDVLRGVRRAMAQNMARAQSEVAAATVMDDADLHAWQATGASVTGQDITIRLVRALVAGVRAEPALNAWYEGRTGRRH
LLERIDVGIAADLPEGLFVPVLRNVGKRDAADLRAGLDRMRADVVARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPT
VAILGAGRVRDEVVASGGVPAVHRVMPLSLTFDHRVVTGGEAARFLRAVIEDLERPE
>Mature_377_residues
MRIFKLPDLGEGLQEAEIVNWHVKPGDTIAADQPLLSVETAKAIVEIPSPFAGQVAKLFAQPGDIVHLGAPLVGFEGAGA
SDDAGTVVGAVKVGTHVVNEGVTRVGAAGIPGGHGIKATPAVRALARKLSVDLSMVTPSGHDGVITATDVQRVATTLADV
GAPDVLRGVRRAMAQNMARAQSEVAAATVMDDADLHAWQATGASVTGQDITIRLVRALVAGVRAEPALNAWYEGRTGRRH
LLERIDVGIAADLPEGLFVPVLRNVGKRDAADLRAGLDRMRADVVARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPT
VAILGAGRVRDEVVASGGVPAVHRVMPLSLTFDHRVVTGGEAARFLRAVIEDLERPE

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=27.8571428571429, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI19923748, Length=241, Percent_Identity=31.1203319502075, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI203098753, Length=443, Percent_Identity=22.5733634311512, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI203098816, Length=443, Percent_Identity=22.3476297968397, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=23.3796296296296, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1786305, Length=410, Percent_Identity=29.0243902439024, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1786946, Length=401, Percent_Identity=23.9401496259352, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=27.6807980049875, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=26.3657957244656, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17560088, Length=427, Percent_Identity=24.1217798594848, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17538894, Length=185, Percent_Identity=27.027027027027, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320352, Length=390, Percent_Identity=25.1282051282051, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324258, Length=186, Percent_Identity=25.8064516129032, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=26.9320843091335, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=28.5067873303167, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582497, Length=183, Percent_Identity=27.8688524590164, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129315, Length=183, Percent_Identity=27.8688524590164, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 39389; Mature: 39389

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIFKLPDLGEGLQEAEIVNWHVKPGDTIAADQPLLSVETAKAIVEIPSPFAGQVAKLFA
CCEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHC
QPGDIVHLGAPLVGFEGAGASDDAGTVVGAVKVGTHVVNEGVTRVGAAGIPGGHGIKATP
CCCCEEEECCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
AVRALARKLSVDLSMVTPSGHDGVITATDVQRVATTLADVGAPDVLRGVRRAMAQNMARA
HHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QSEVAAATVMDDADLHAWQATGASVTGQDITIRLVRALVAGVRAEPALNAWYEGRTGRRH
HHHHHHHHHCCCCCCHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHH
LLERIDVGIAADLPEGLFVPVLRNVGKRDAADLRAGLDRMRADVVARTIAPEEMRGNTIT
HHHHHCCCEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEE
LSNFGMIAGRYAAPIVVPPTVAILGAGRVRDEVVASGGVPAVHRVMPLSLTFDHRVVTGG
EECCCCCHHCCCCCEEECCCEEEEECCHHHHHHHHCCCCCHHHHHEEEEEEECCEEECCC
EAARFLRAVIEDLERPE
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIFKLPDLGEGLQEAEIVNWHVKPGDTIAADQPLLSVETAKAIVEIPSPFAGQVAKLFA
CCEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHC
QPGDIVHLGAPLVGFEGAGASDDAGTVVGAVKVGTHVVNEGVTRVGAAGIPGGHGIKATP
CCCCEEEECCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
AVRALARKLSVDLSMVTPSGHDGVITATDVQRVATTLADVGAPDVLRGVRRAMAQNMARA
HHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QSEVAAATVMDDADLHAWQATGASVTGQDITIRLVRALVAGVRAEPALNAWYEGRTGRRH
HHHHHHHHHCCCCCCHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHH
LLERIDVGIAADLPEGLFVPVLRNVGKRDAADLRAGLDRMRADVVARTIAPEEMRGNTIT
HHHHHCCCEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEE
LSNFGMIAGRYAAPIVVPPTVAILGAGRVRDEVVASGGVPAVHRVMPLSLTFDHRVVTGG
EECCCCCHHCCCCCEEECCCEEEEECCHHHHHHHHCCCCCHHHHHEEEEEEECCEEECCC
EAARFLRAVIEDLERPE
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]