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The map label for this gene is napA [H]

Identifier: 94312991

GI number: 94312991

Start: 627862

End: 630357

Strand: Reverse

Name: napA [H]

Synonym: Rmet_4063

Alternate gene names: 94312991

Gene position: 630357-627862 (Counterclockwise)

Preceding gene: 94312992

Following gene: 94312990

Centisome position: 24.43

GC content: 63.26

Gene sequence:

>2496_bases
ATGCCACTCACGCGCCGTGATTTCATCAAGCAGACAGCCATCGCCGCCACGGCCTCGGTGGCGGGCGTCTCGCTGCCGAC
GGATGCGGCCAATTTCGTGACGGACAGCGAAGTCACGAAACTGAAATGGTCGAAGGCGCCCTGCCGCTTCTGCGGCACGG
GGTGCGGTGTGACCGTCGCGGTCAAGGACAACAAGGTGGTGGCCACCCAGGGCGACCCGCTTTGCGAGGTCAACAAGGGC
CTGAATTGCGTGAAGGGCTACTTCCTGTCGAAGATCATGTATGGGCAGGACCGCCTGACCAAGCCGCTGCTGCGGATGAA
GAACGGCAAGTACGACAAGAACGGCGAATTCGCGCCGGTCAGCTGGGATCGCGCCTTCGACGAGATGGAGGCCCAGTTCA
AGCGCGTGCTCAAGGAGAAAGGCCCGACCGCCGTGGGCATGTTCGGCTCCGGCCAGTGGACCGTATGGGAAGGCTACGCC
GCTTCCAAGCTGTACAAGGCGGGGTTCCGCTCCAACAACATCGACCCGAACGCGCGCCATTGCATGGCGTCGGCCGTGAC
CGGCTTCATGCGCACCTTCGGCATGGACGAGCCGATGGGCTGCTATGACGACATCGAGGCCGCCGACGTCTTTGTGCTGT
GGGGCTCCAATATGGCGGAGATGCACCCGATCCTGTGGACACGCATCACGGACCGCCGCCTGAGCCACCCCAAGACCCGC
GTGGCCGTGCTGTCCACGTTCACGCATCGTTCGTTCGACCTGGCCGATATCCCGGTCATCTTCAAGCCCCAGACCGACCT
GGCGATGCTGAACTTTATCGCCAACTACATCATCCGCAATAACAAGGTCAACAAGGACTTCGTCAACAAGTACACGGTGT
TCAAGGAAGGCGTGACCGATATCGGCTATGGCCTGCGCCCGGACAATCCGCTCCAGAAGGCGGCCAAGAACGCCGGCAAC
CCGAACGACTCCAAGCTGATTACGTTCGACGACTTCGCGAAGTTCGTGTCGAAGTACGATGCTGACTACGTCAGCAAGCT
CTCCGGCGTGCCCAAGAAGCAGCTCGAGCAACTGGCCGAGCTTTATGCCGACCCGAATCTCAAGGTCATGTCGCTGTGGA
CCATGGGTTTCAACCAGCACACGCGCGGCACGTGGGTCAACAACATGGCCTACAACGTCCACCTGCTGACGGGCAAGATC
GCCACGCCGGGCAACAGTCCGTTCTCGCTGACAGGGCAGCCATCCGCCTGCGGCACGGCACGGGAGGTAGGCACGTTCTC
GCACCGGCTGCCGGCCGACATGGTCGTCACCAATCCCAAGCACCGCGAGGAAGCCGAACGTATCTGGAAGCTGCCGCCGG
GGACTATCCCCGACAAGCCCGGCTACCACGCCGTCCTGCAGAACCGGATGCTCAGGGACGGCAAGCTCAACGCGTACTGG
GTGCAGGTCAACAACAACATGCAGGCGGCAGCCAACATGATGGAGGAGACCCTGCCCGGCTACCGCAATCCGACGAACTT
CATCGTCGTCTCCGACGCCTACCCGACGGTCACCGCGCTGTCGGCCGACCTGATCCTGCCCACCGCGATGTGGGTCGAAA
AGGAAGGCGCGTACGGTAACGCGGAACGCCGCACGCAGTTCTGGCACCAGCTCGTAGACGCCCCGGGCGAGGCGCGCTCC
GATCTCTGGCAGTTGATGGAATTCTCGAAGCGCTTCAAGGTCGAGGACGTCTGGCCGGCGGAACTGATCGCGAAGAAACC
CGAGTACAAGGGCAAGACGCTCTTCGACGTGCTGTATCGCAACGGCCAGGTCGACAAGTTCCCGCTCAAGGAAACCGCCT
CCGACTACAACAACTACGAGTCCAAGGCTTTCGGCTTCTACGTCCAGAAGGGCCTGTTCGAGGAATACGCGACCTTCGGC
CGTGGCCACGGCCACGACCTGGCGCCGTTCGACATGTACCACGAGGCACGCGGCTTGCGCTGGCCGGTGGTCAACGGCAA
GGAAACGCGCTGGCGCTATCGCGAAGGCTCGGACCCGTACGTGAAGGCGGGTACCGGCTTCCAGTTCTACGGCAACCCGG
ACGGCAAGGCCGTGATCTACGCCCTGCCCTACGAGCCGCCGGCCGAGTCCCCGGACAAGGAATACCCGTTCTGGCTGGCC
ACGGGCCGCGTGCTCGAACACTGGCACTCCGGCTCGATGACGCGGCGCGTGCCGGAGCTGTACCGCGCCTTCCCGAACGC
GGTGTGCTTCATGCATCCGGAAGACGCCAAGGCACTGGGGCTGCGGCGCGGGGTGGAAGTGGAAGTGGTCTCGCGTCGCG
GCCGTATGCGCACGCGCATCGAAACCCGCGGACGCGACGCGCCGCCGCGCGGTCTGGTATTCGTGCCCTGGTTCGATGCC
AGCCAGCTGATCAACAAGGTCACGCTCGACGCCACCTGCCCGATCTCGCTGCAGACCGACTTCAAGAAGTGCGCGGTCAA
GATCGTCAAGGTATGA

Upstream 100 bases:

>100_bases
GCGCAACTCACCTGTCTGCATCAACTCGAAGGCGTCTTTTCCGCCACGCTGGTCTACCAGCACAACGAAGATGCCGCCGC
CATGAACGAGGAGATCTCCG

Downstream 100 bases:

>100_bases
GTACAGGAGGACACGCCATGTTGCCAAGCCGATCCTGGGTCCCCCTGCCCCTGCTGGTCCTCATGCTGGTGGTCATGCTG
CTCAGCGTGCCGCTGGCGCG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 830

Protein sequence:

>831_residues
MPLTRRDFIKQTAIAATASVAGVSLPTDAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVKDNKVVATQGDPLCEVNKG
LNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPVSWDRAFDEMEAQFKRVLKEKGPTAVGMFGSGQWTVWEGYA
ASKLYKAGFRSNNIDPNARHCMASAVTGFMRTFGMDEPMGCYDDIEAADVFVLWGSNMAEMHPILWTRITDRRLSHPKTR
VAVLSTFTHRSFDLADIPVIFKPQTDLAMLNFIANYIIRNNKVNKDFVNKYTVFKEGVTDIGYGLRPDNPLQKAAKNAGN
PNDSKLITFDDFAKFVSKYDADYVSKLSGVPKKQLEQLAELYADPNLKVMSLWTMGFNQHTRGTWVNNMAYNVHLLTGKI
ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYHAVLQNRMLRDGKLNAYW
VQVNNNMQAAANMMEETLPGYRNPTNFIVVSDAYPTVTALSADLILPTAMWVEKEGAYGNAERRTQFWHQLVDAPGEARS
DLWQLMEFSKRFKVEDVWPAELIAKKPEYKGKTLFDVLYRNGQVDKFPLKETASDYNNYESKAFGFYVQKGLFEEYATFG
RGHGHDLAPFDMYHEARGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIYALPYEPPAESPDKEYPFWLA
TGRVLEHWHSGSMTRRVPELYRAFPNAVCFMHPEDAKALGLRRGVEVEVVSRRGRMRTRIETRGRDAPPRGLVFVPWFDA
SQLINKVTLDATCPISLQTDFKKCAVKIVKV

Sequences:

>Translated_831_residues
MPLTRRDFIKQTAIAATASVAGVSLPTDAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVKDNKVVATQGDPLCEVNKG
LNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPVSWDRAFDEMEAQFKRVLKEKGPTAVGMFGSGQWTVWEGYA
ASKLYKAGFRSNNIDPNARHCMASAVTGFMRTFGMDEPMGCYDDIEAADVFVLWGSNMAEMHPILWTRITDRRLSHPKTR
VAVLSTFTHRSFDLADIPVIFKPQTDLAMLNFIANYIIRNNKVNKDFVNKYTVFKEGVTDIGYGLRPDNPLQKAAKNAGN
PNDSKLITFDDFAKFVSKYDADYVSKLSGVPKKQLEQLAELYADPNLKVMSLWTMGFNQHTRGTWVNNMAYNVHLLTGKI
ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYHAVLQNRMLRDGKLNAYW
VQVNNNMQAAANMMEETLPGYRNPTNFIVVSDAYPTVTALSADLILPTAMWVEKEGAYGNAERRTQFWHQLVDAPGEARS
DLWQLMEFSKRFKVEDVWPAELIAKKPEYKGKTLFDVLYRNGQVDKFPLKETASDYNNYESKAFGFYVQKGLFEEYATFG
RGHGHDLAPFDMYHEARGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIYALPYEPPAESPDKEYPFWLA
TGRVLEHWHSGSMTRRVPELYRAFPNAVCFMHPEDAKALGLRRGVEVEVVSRRGRMRTRIETRGRDAPPRGLVFVPWFDA
SQLINKVTLDATCPISLQTDFKKCAVKIVKV
>Mature_830_residues
PLTRRDFIKQTAIAATASVAGVSLPTDAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVKDNKVVATQGDPLCEVNKGL
NCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPVSWDRAFDEMEAQFKRVLKEKGPTAVGMFGSGQWTVWEGYAA
SKLYKAGFRSNNIDPNARHCMASAVTGFMRTFGMDEPMGCYDDIEAADVFVLWGSNMAEMHPILWTRITDRRLSHPKTRV
AVLSTFTHRSFDLADIPVIFKPQTDLAMLNFIANYIIRNNKVNKDFVNKYTVFKEGVTDIGYGLRPDNPLQKAAKNAGNP
NDSKLITFDDFAKFVSKYDADYVSKLSGVPKKQLEQLAELYADPNLKVMSLWTMGFNQHTRGTWVNNMAYNVHLLTGKIA
TPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYHAVLQNRMLRDGKLNAYWV
QVNNNMQAAANMMEETLPGYRNPTNFIVVSDAYPTVTALSADLILPTAMWVEKEGAYGNAERRTQFWHQLVDAPGEARSD
LWQLMEFSKRFKVEDVWPAELIAKKPEYKGKTLFDVLYRNGQVDKFPLKETASDYNNYESKAFGFYVQKGLFEEYATFGR
GHGHDLAPFDMYHEARGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIYALPYEPPAESPDKEYPFWLAT
GRVLEHWHSGSMTRRVPELYRAFPNAVCFMHPEDAKALGLRRGVEVEVVSRRGRMRTRIETRGRDAPPRGLVFVPWFDAS
QLINKVTLDATCPISLQTDFKKCAVKIVKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=831, Percent_Identity=70.0361010830325, Blast_Score=1247, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=796, Percent_Identity=23.8693467336683, Blast_Score=203, Evalue=5e-53,
Organism=Escherichia coli, GI3868720, Length=663, Percent_Identity=22.7752639517345, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI3868719, Length=659, Percent_Identity=23.6722306525038, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787870, Length=598, Percent_Identity=23.5785953177258, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87081797, Length=605, Percent_Identity=22.3140495867769, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI171474008, Length=816, Percent_Identity=22.7941176470588, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93726; Mature: 93595

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTRRDFIKQTAIAATASVAGVSLPTDAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHEEECCCCCHHHCCCCCCEEEE
VKDNKVVATQGDPLCEVNKGLNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPV
EECCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
SWDRAFDEMEAQFKRVLKEKGPTAVGMFGSGQWTVWEGYAASKLYKAGFRSNNIDPNARH
CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVTGFMRTFGMDEPMGCYDDIEAADVFVLWGSNMAEMHPILWTRITDRRLSHPKTR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCHHHHEEEHHHHHCCCHHH
VAVLSTFTHRSFDLADIPVIFKPQTDLAMLNFIANYIIRNNKVNKDFVNKYTVFKEGVTD
EEEEEHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHH
IGYGLRPDNPLQKAAKNAGNPNDSKLITFDDFAKFVSKYDADYVSKLSGVPKKQLEQLAE
CCCCCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
LYADPNLKVMSLWTMGFNQHTRGTWVNNMAYNVHLLTGKIATPGNSPFSLTGQPSACGTA
HHCCCCCEEEEEEEECCCCCCCCCEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCH
REVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYHAVLQNRMLRDGKLNAYW
HHHCCHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
VQVNNNMQAAANMMEETLPGYRNPTNFIVVSDAYPTVTALSADLILPTAMWVEKEGAYGN
EEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCC
AERRTQFWHQLVDAPGEARSDLWQLMEFSKRFKVEDVWPAELIAKKPEYKGKTLFDVLYR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHC
NGQVDKFPLKETASDYNNYESKAFGFYVQKGLFEEYATFGRGHGHDLAPFDMYHEARGLR
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC
WPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIYALPYEPPAESPDKEYPFWLA
CCCCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEE
TGRVLEHWHSGSMTRRVPELYRAFPNAVCFMHPEDAKALGLRRGVEVEVVSRRGRMRTRI
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCEEEEEHHCCCHHHHH
ETRGRDAPPRGLVFVPWFDASQLINKVTLDATCPISLQTDFKKCAVKIVKV
HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCEECCCCHHHHHHEEECC
>Mature Secondary Structure 
PLTRRDFIKQTAIAATASVAGVSLPTDAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVA
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHEEECCCCCHHHCCCCCCEEEE
VKDNKVVATQGDPLCEVNKGLNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPV
EECCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
SWDRAFDEMEAQFKRVLKEKGPTAVGMFGSGQWTVWEGYAASKLYKAGFRSNNIDPNARH
CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVTGFMRTFGMDEPMGCYDDIEAADVFVLWGSNMAEMHPILWTRITDRRLSHPKTR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCHHHHEEEHHHHHCCCHHH
VAVLSTFTHRSFDLADIPVIFKPQTDLAMLNFIANYIIRNNKVNKDFVNKYTVFKEGVTD
EEEEEHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHH
IGYGLRPDNPLQKAAKNAGNPNDSKLITFDDFAKFVSKYDADYVSKLSGVPKKQLEQLAE
CCCCCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
LYADPNLKVMSLWTMGFNQHTRGTWVNNMAYNVHLLTGKIATPGNSPFSLTGQPSACGTA
HHCCCCCEEEEEEEECCCCCCCCCEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCH
REVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYHAVLQNRMLRDGKLNAYW
HHHCCHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
VQVNNNMQAAANMMEETLPGYRNPTNFIVVSDAYPTVTALSADLILPTAMWVEKEGAYGN
EEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCC
AERRTQFWHQLVDAPGEARSDLWQLMEFSKRFKVEDVWPAELIAKKPEYKGKTLFDVLYR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHC
NGQVDKFPLKETASDYNNYESKAFGFYVQKGLFEEYATFGRGHGHDLAPFDMYHEARGLR
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC
WPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIYALPYEPPAESPDKEYPFWLA
CCCCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEE
TGRVLEHWHSGSMTRRVPELYRAFPNAVCFMHPEDAKALGLRRGVEVEVVSRRGRMRTRI
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCEEEEEHHCCCHHHHH
ETRGRDAPPRGLVFVPWFDASQLINKVTLDATCPISLQTDFKKCAVKIVKV
HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCEECCCCHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]