Click here to switch to the map view.

The map label for this gene is napB [H]

Identifier: 94312990

GI number: 94312990

Start: 627353

End: 627844

Strand: Reverse

Name: napB [H]

Synonym: Rmet_4062

Alternate gene names: 94312990

Gene position: 627844-627353 (Counterclockwise)

Preceding gene: 94312991

Following gene: 94312989

Centisome position: 24.33

GC content: 63.41

Gene sequence:

>492_bases
ATGTTGCCAAGCCGATCCTGGGTCCCCCTGCCCCTGCTGGTCCTCATGCTGGTGGTCATGCTGCTCAGCGTGCCGCTGGC
GCGCGCGCAGGGCCTGGTGGACTCGATGCGTGGCCCCACGCCGGTGGCCAACGAGACCTCGCCGCCAATACTGTTCCCGA
CCGAGAACAAGGACATCCGCCGTACCCGCAACTACACGATGCAACCGCCGACGATCCCCCACAAGATCGACAGCTACCAG
CTCGACAAGGACTTCAACCGCTGCATGTTCTGCCACGCGCGCACGCGCACGGAGGAAACGCAGGCCATCCCGGTCAGCAT
CACGCACTACATGGATCGTGACAACAACGTGCTGGCCGAGATCTCGCCACGGCGCTACTTCTGCACGCAATGCCACGTGC
CGCAGGCCGACGCCAAGCCACTGGTGAACAACACGTTCGTCGATGTGGAGGCGATCCTCAAACGCAGTGGCGGCACCAAG
GGTGCCAAATAA

Upstream 100 bases:

>100_bases
ACAAGGTCACGCTCGACGCCACCTGCCCGATCTCGCTGCAGACCGACTTCAAGAAGTGCGCGGTCAAGATCGTCAAGGTA
TGAGTACAGGAGGACACGCC

Downstream 100 bases:

>100_bases
CCAGGCGACGGTGACCGATCATGTGGGACCTGATCAAGCGCTACTGGCATACCATCAACCGCCCCAGTGCCTACTTCAGC
CTGGGATTCCTGACCCTGGG

Product: Nitrate reductase cytochrome c-type subunit

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MLPSRSWVPLPLLVLMLVVMLLSVPLARAQGLVDSMRGPTPVANETSPPILFPTENKDIRRTRNYTMQPPTIPHKIDSYQ
LDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLAEISPRRYFCTQCHVPQADAKPLVNNTFVDVEAILKRSGGTK
GAK

Sequences:

>Translated_163_residues
MLPSRSWVPLPLLVLMLVVMLLSVPLARAQGLVDSMRGPTPVANETSPPILFPTENKDIRRTRNYTMQPPTIPHKIDSYQ
LDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLAEISPRRYFCTQCHVPQADAKPLVNNTFVDVEAILKRSGGTK
GAK
>Mature_163_residues
MLPSRSWVPLPLLVLMLVVMLLSVPLARAQGLVDSMRGPTPVANETSPPILFPTENKDIRRTRNYTMQPPTIPHKIDSYQ
LDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLAEISPRRYFCTQCHVPQADAKPLVNNTFVDVEAILKRSGGTK
GAK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=138, Percent_Identity=41.304347826087, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 18432; Mature: 18432

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSRSWVPLPLLVLMLVVMLLSVPLARAQGLVDSMRGPTPVANETSPPILFPTENKDIR
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHH
RTRNYTMQPPTIPHKIDSYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLAE
HHCCCCCCCCCCCCCCCCEECCCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEE
ISPRRYFCTQCHVPQADAKPLVNNTFVDVEAILKRSGGTKGAK
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLPSRSWVPLPLLVLMLVVMLLSVPLARAQGLVDSMRGPTPVANETSPPILFPTENKDIR
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHH
RTRNYTMQPPTIPHKIDSYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLAE
HHCCCCCCCCCCCCCCCCEECCCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEE
ISPRRYFCTQCHVPQADAKPLVNNTFVDVEAILKRSGGTKGAK
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]