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The map label for this gene is arsB1 [H]

Identifier: 94312919

GI number: 94312919

Start: 559311

End: 560570

Strand: Reverse

Name: arsB1 [H]

Synonym: Rmet_3991

Alternate gene names: 94312919

Gene position: 560570-559311 (Counterclockwise)

Preceding gene: 94312920

Following gene: 94312918

Centisome position: 21.73

GC content: 66.9

Gene sequence:

>1260_bases
ATGCCCGCCTACGCGACACCGCTGATCTGGAGCGTCGCGGCACTATCCACGGCGGGCGTGCTGTTCCGGCCCTTTCGCCT
GCCCGAGCCGTTCTGGGCAATGGCTGGCGCGCTGGTGCTATGCGTGGCAGGACTGCTGCCATGGCGCGACGCCCTGCAAG
CCGTGGCACGCGGCAACGACGTCTATCTGTTCCTGGCCGGGATGATCCTGATCTCGGAACTCGCCCGCAAGACAGGTCTG
TTCGATCATGTGGCCGCGCTGGCCGTGCGCGCCGCGCGGGGGTCGGCGCGCAAGCTATTTGCGCTGGTCTATGGCTTCGG
CATTGCGGTGACAGCGTTCATGTCGAACGATGCCACGGCAGTCGTCCTCACCCCCGCGGTCATCGCCGCGACACGCGCGG
CACGGGTCAAGCACCCGTTACCCTATCTCTACGCCTGCGCGTTCATCGCCAATGCGGCGAGCTTTCTCCTGCCGATCTCT
AATCCGGCCAACCTCGTGCTGTTCGGTGACCGCATGCCACCTCTGACCAGTTGGCTGGCACGCTTCACGCTGCCGTCGGT
GGTGGCCATCGCCATGACGTTCATCGTCCTGTACTGGACGCAACGCGATGCACTGGCCGAGCCGATTGAGAACGACGTGC
CGACGCCCCCTCTGACGCTCCAGGCCTGGCTGACGACGCTGGGCATCATGCTGACCGGAGCGGCACTGTTGACGGCCTCG
CTGCACGGGCAGGATCTCGGCTGGCCGACGTTCATCGGTGGTCTGTTGACCCTGGCTGTCGTCTGCGCCACCCAGCCGCG
ACTGCTTGTGCCGGCGCTCAAGGAGGTGTCCTGGGGCGTATTGCCGTTGGTGGCCGGACTGTTCGTCCTGGTTGCCGGCC
TGGCCCAGACCGGCTTGACCGCTCAGCTTGCACATTGGGTGCGGATGCTATCCGGGCTGCAAGGGCCGGAGGCCGTGCTT
GGCGCCGGTGTGGCGGGCGTGCTCGTCGGCATCACGAGCAACATCGTCAACAACCTGCCGGCCGGACTGTTCGCAGCCTC
GGCGCTGGCGGCAGGCCACGCCTCTGATACCGTCACGGCTGCCGTGCTGATCGGTGTGGACCTGGGCCCGAACCTGTCCA
TTACCGGCTCGCTGGCCACCCTGCTCTGGCTGACCGCCCTGCGCCGTGAAGGTCATATGGTCGGCGCCGGCACCTTCCTG
AAGACCGGTGCGCTCGTCATGCCGCTGGCACTACTCCCGGCCCTGGCGGTACTGCGCTGA

Upstream 100 bases:

>100_bases
GCGCACCGACAGCCGCGGCGCCGACCCCTGCAGCGCTTGAGGTATCGCCGCAGGTATCGTCCCAACGTCCTGCCGCTACC
GGCCTGTGACCACGGCCGAC

Downstream 100 bases:

>100_bases
GGCCATGCCCAGCAGCGGGCTGTTGCAGGTTGTGGTGCAATGCCAACTGCCATATAGTGAAGCACCGCGCACCGCGTGCG
CCGACGCCCAACTCCCTGCG

Product: Arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPAYATPLIWSVAALSTAGVLFRPFRLPEPFWAMAGALVLCVAGLLPWRDALQAVARGNDVYLFLAGMILISELARKTGL
FDHVAALAVRAARGSARKLFALVYGFGIAVTAFMSNDATAVVLTPAVIAATRAARVKHPLPYLYACAFIANAASFLLPIS
NPANLVLFGDRMPPLTSWLARFTLPSVVAIAMTFIVLYWTQRDALAEPIENDVPTPPLTLQAWLTTLGIMLTGAALLTAS
LHGQDLGWPTFIGGLLTLAVVCATQPRLLVPALKEVSWGVLPLVAGLFVLVAGLAQTGLTAQLAHWVRMLSGLQGPEAVL
GAGVAGVLVGITSNIVNNLPAGLFAASALAAGHASDTVTAAVLIGVDLGPNLSITGSLATLLWLTALRREGHMVGAGTFL
KTGALVMPLALLPALAVLR

Sequences:

>Translated_419_residues
MPAYATPLIWSVAALSTAGVLFRPFRLPEPFWAMAGALVLCVAGLLPWRDALQAVARGNDVYLFLAGMILISELARKTGL
FDHVAALAVRAARGSARKLFALVYGFGIAVTAFMSNDATAVVLTPAVIAATRAARVKHPLPYLYACAFIANAASFLLPIS
NPANLVLFGDRMPPLTSWLARFTLPSVVAIAMTFIVLYWTQRDALAEPIENDVPTPPLTLQAWLTTLGIMLTGAALLTAS
LHGQDLGWPTFIGGLLTLAVVCATQPRLLVPALKEVSWGVLPLVAGLFVLVAGLAQTGLTAQLAHWVRMLSGLQGPEAVL
GAGVAGVLVGITSNIVNNLPAGLFAASALAAGHASDTVTAAVLIGVDLGPNLSITGSLATLLWLTALRREGHMVGAGTFL
KTGALVMPLALLPALAVLR
>Mature_418_residues
PAYATPLIWSVAALSTAGVLFRPFRLPEPFWAMAGALVLCVAGLLPWRDALQAVARGNDVYLFLAGMILISELARKTGLF
DHVAALAVRAARGSARKLFALVYGFGIAVTAFMSNDATAVVLTPAVIAATRAARVKHPLPYLYACAFIANAASFLLPISN
PANLVLFGDRMPPLTSWLARFTLPSVVAIAMTFIVLYWTQRDALAEPIENDVPTPPLTLQAWLTTLGIMLTGAALLTASL
HGQDLGWPTFIGGLLTLAVVCATQPRLLVPALKEVSWGVLPLVAGLFVLVAGLAQTGLTAQLAHWVRMLSGLQGPEAVLG
AGVAGVLVGITSNIVNNLPAGLFAASALAAGHASDTVTAAVLIGVDLGPNLSITGSLATLLWLTALRREGHMVGAGTFLK
TGALVMPLALLPALAVLR

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=366, Percent_Identity=28.9617486338798, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 43666; Mature: 43535

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAYATPLIWSVAALSTAGVLFRPFRLPEPFWAMAGALVLCVAGLLPWRDALQAVARGND
CCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
VYLFLAGMILISELARKTGLFDHVAALAVRAARGSARKLFALVYGFGIAVTAFMSNDATA
EEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VVLTPAVIAATRAARVKHPLPYLYACAFIANAASFLLPISNPANLVLFGDRMPPLTSWLA
EEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCHHHHHHH
RFTLPSVVAIAMTFIVLYWTQRDALAEPIENDVPTPPLTLQAWLTTLGIMLTGAALLTAS
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LHGQDLGWPTFIGGLLTLAVVCATQPRLLVPALKEVSWGVLPLVAGLFVLVAGLAQTGLT
HCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHH
AQLAHWVRMLSGLQGPEAVLGAGVAGVLVGITSNIVNNLPAGLFAASALAAGHASDTVTA
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHE
AVLIGVDLGPNLSITGSLATLLWLTALRREGHMVGAGTFLKTGALVMPLALLPALAVLR
EHEEEECCCCCCEEHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAYATPLIWSVAALSTAGVLFRPFRLPEPFWAMAGALVLCVAGLLPWRDALQAVARGND
CCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
VYLFLAGMILISELARKTGLFDHVAALAVRAARGSARKLFALVYGFGIAVTAFMSNDATA
EEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VVLTPAVIAATRAARVKHPLPYLYACAFIANAASFLLPISNPANLVLFGDRMPPLTSWLA
EEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCHHHHHHH
RFTLPSVVAIAMTFIVLYWTQRDALAEPIENDVPTPPLTLQAWLTTLGIMLTGAALLTAS
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LHGQDLGWPTFIGGLLTLAVVCATQPRLLVPALKEVSWGVLPLVAGLFVLVAGLAQTGLT
HCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHH
AQLAHWVRMLSGLQGPEAVLGAGVAGVLVGITSNIVNNLPAGLFAASALAAGHASDTVTA
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHE
AVLIGVDLGPNLSITGSLATLLWLTALRREGHMVGAGTFLKTGALVMPLALLPALAVLR
EHEEEECCCCCCEEHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA