Click here to switch to the map view.

The map label for this gene is folE [H]

Identifier: 94312918

GI number: 94312918

Start: 558478

End: 559203

Strand: Reverse

Name: folE [H]

Synonym: Rmet_3990

Alternate gene names: 94312918

Gene position: 559203-558478 (Counterclockwise)

Preceding gene: 94312919

Following gene: 94312917

Centisome position: 21.67

GC content: 61.43

Gene sequence:

>726_bases
ATGTCACACAAGGATCAGTCCGATCGCCCCGAGCACACGCCCACCGCCTCGCCCGTATCCAACCGGATTCGGGCGCGCAT
CGAGGCTGCCGGCGAGCGCTTCCACGCCAACGACAATATCTCTCGCCATATCGAGCACGGTGAACTCGAAGCCCTGCAGG
CCGAGGTCGAAGCCCGCATGGAAGACGTATTACGCACGCTGGTGATCGACGTCGACCAGGATCACAACACACGCGAGACC
GCTCGCCGCGTAGCCAAGATGTATCTCAAGGAGATCTTCGCGGGACGCTACGCGAAGGCGCCGGATGTGACCGAGTTTCC
GAACGTGGAACAGCTCAACGAGCTGATGATCGTCGGGCCCATCCGCGTGCGCAGCGCCTGCTCTCATCACCTGTGCCCGA
TCATCGGCAGCTTGTGGGTGGGGGTGATGCCGAACCGCCACTCCAATCTGATCGGCCTGTCCAAGTATGCGCGACTGGCG
GAATGGATCATGTGCCGGCCGCAGATCCAGGAAGAAGCCGTGGCACAGGTCGCCGATCTGCTGCAGGAGAAGATGAATCC
AGACGGCCTGGCAATCGTCATGGAAGCCGAGCACTTCTGCATGCACTGGCGTGGCGTGCGCGATACCGACGCCAAGATGA
CCAACAGCGTCATGCGCGGCTCGTTCCTCAAGGACGACAAGCTGCGCCGTGAATTTCTCACCCTGCTGAATCTCAATCGC
CGCTGA

Upstream 100 bases:

>100_bases
CCCAGCAGCGGGCTGTTGCAGGTTGTGGTGCAATGCCAACTGCCATATAGTGAAGCACCGCGCACCGCGTGCGCCGACGC
CCAACTCCCTGCGGCCACAC

Downstream 100 bases:

>100_bases
CTCGCTGCCCGGAGGGAACACCGATGCTAGTCCGCCTGCTCTACGCCAGCCGCGCCAGACAACCGCTTGACGCCGATGTG
CTGGACGCCATCCTCGACAC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSHKDQSDRPEHTPTASPVSNRIRARIEAAGERFHANDNISRHIEHGELEALQAEVEARMEDVLRTLVIDVDQDHNTRET
ARRVAKMYLKEIFAGRYAKAPDVTEFPNVEQLNELMIVGPIRVRSACSHHLCPIIGSLWVGVMPNRHSNLIGLSKYARLA
EWIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDDKLRREFLTLLNLNR
R

Sequences:

>Translated_241_residues
MSHKDQSDRPEHTPTASPVSNRIRARIEAAGERFHANDNISRHIEHGELEALQAEVEARMEDVLRTLVIDVDQDHNTRET
ARRVAKMYLKEIFAGRYAKAPDVTEFPNVEQLNELMIVGPIRVRSACSHHLCPIIGSLWVGVMPNRHSNLIGLSKYARLA
EWIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDDKLRREFLTLLNLNR
R
>Mature_240_residues
SHKDQSDRPEHTPTASPVSNRIRARIEAAGERFHANDNISRHIEHGELEALQAEVEARMEDVLRTLVIDVDQDHNTRETA
RRVAKMYLKEIFAGRYAKAPDVTEFPNVEQLNELMIVGPIRVRSACSHHLCPIIGSLWVGVMPNRHSNLIGLSKYARLAE
WIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDDKLRREFLTLLNLNRR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=127, Percent_Identity=41.7322834645669, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI4503949, Length=127, Percent_Identity=41.7322834645669, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI66932970, Length=97, Percent_Identity=36.0824742268041, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI66932972, Length=87, Percent_Identity=39.0804597701149, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788476, Length=185, Percent_Identity=32.972972972973, Blast_Score=110, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=128, Percent_Identity=42.1875, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=37.1584699453552, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24656791, Length=128, Percent_Identity=39.0625, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24656796, Length=128, Percent_Identity=39.0625, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24656782, Length=128, Percent_Identity=39.0625, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 27639; Mature: 27508

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKDQSDRPEHTPTASPVSNRIRARIEAAGERFHANDNISRHIEHGELEALQAEVEARM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
EDVLRTLVIDVDQDHNTRETARRVAKMYLKEIFAGRYAKAPDVTEFPNVEQLNELMIVGP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEECC
IRVRSACSHHLCPIIGSLWVGVMPNRHSNLIGLSKYARLAEWIMCRPQIQEEAVAQVADL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDDKLRREFLTLLNLNR
HHHHCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
R
C
>Mature Secondary Structure 
SHKDQSDRPEHTPTASPVSNRIRARIEAAGERFHANDNISRHIEHGELEALQAEVEARM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
EDVLRTLVIDVDQDHNTRETARRVAKMYLKEIFAGRYAKAPDVTEFPNVEQLNELMIVGP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEECC
IRVRSACSHHLCPIIGSLWVGVMPNRHSNLIGLSKYARLAEWIMCRPQIQEEAVAQVADL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDDKLRREFLTLLNLNR
HHHHCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA