Click here to switch to the map view.

The map label for this gene is mrcB [H]

Identifier: 94312920

GI number: 94312920

Start: 560582

End: 562645

Strand: Reverse

Name: mrcB [H]

Synonym: Rmet_3992

Alternate gene names: 94312920

Gene position: 562645-560582 (Counterclockwise)

Preceding gene: 94312921

Following gene: 94312919

Centisome position: 21.81

GC content: 66.28

Gene sequence:

>2064_bases
ATGCGCGCCATGAAAAAGATTGCAGTCAAGGCTGCCGGCGTCGTCGCGGCGCTGGGAGCCGCCGGCGTCCTGCTCGTCGG
CTTTGCCGCGGTAGTCAGCCTGCGGCAACTTCCCTCGGTCGACGCATTGCGCGACTATCGTCCCCAGGTACCGCTGCGCG
TGTTCACCAGCGACGATGTTCTCATTGGCGAGTTTGGCGCCGAGCATCGAGAGTTCATCCCGATCGAGAGAATGCCGAAG
CTGATGACCGAGGCACTACTCGCGGCCGAGGACGACCAGTTCTACAACCATGCCGGCGTGGACATCCCCGGCCTGGTCCG
CGCGGCGCTGGCAGACCTCGGCGAGGGCTACGCGCAGGGCGCATCCACCATCACGATGCAGGTGGCGCGCAACTTCTACC
TGTCCCGCGAAAAGACGCTCGCCCGCAAATGGTACGAAGTCCTGCTGGCATGGCAGATCGACCGCACGCTGCCCAAGGAC
CGCGTCCTTGAGCTCTACATGAACCAGGTCTACCTGGGCGAGCACGCCTACGGCTTCGGCAGCGCCGCGCAGACGTACTT
CGGCAAGCCGCTGGCGTCGCTCTCTCTGGCGGAGACCGCAATGCTGGCGGGGCTGCCCAAGGCACCCTCGACGATGAATC
CGCAGGTGAACTACCCGCGCGCCAAACGTCGCCAGGAGTATGTGCTCGGCCGGATGCTGGCGCTGGGCATGATCACGCAG
GACCAGTATCGCGAGGCGCGCGGCGCGCAACTCGTAGTGAGCCGCGACGCCAACGGCAGCTTCAGCGGGCACGCGGAGTA
CGTGGCCGAACTGGCACGGCAACTGGCGTTCGACCGCTTCGGCGACGAAGCCTATACGCGCGGCATCAATGTCTACACCA
CGGTGTCGTCGGTGCGCCAGACATTGGCGTACGACGCCGTGCAGGCCGGACTGGCCAGTTACGGCAAGCGGCACGGCTCG
GCGAAGGCACAGGTCGCGCTGCCCCAGGCGGCGCTGGTCTCGCTCGATGCGAGCACCGGCGCGATTGAAGCCATGGTGGG
TGGCGCCAGTTTCGATACGAGCCGGTTCAACCACGCCACGCAGGCACAGCGCCAGCCGGGATCCACGTTCAAGCCGTTCA
TCTATTCGGCCGCGCTGGAGCGCGGCATTTCGCCCGGCACGCTGATCAACGACGCGCCGCTCGACGACCCGCGCTGGCAG
CCGTCGAATGATGACAATCGCTTCGTCGGCCCGATCACGGTACGTCAGGCGCTGGCGGAATCGCGCAACCTGCCGGCTAT
CCGCACGCTGCGCGCCATTGGCATCCCCTACGCAATCGACTTCGCGGGCAAGTTCGGCTTCTCCACCAAGCGGCTGCCGC
CGTATCTGCCGCTTGCGCTGGGCACGGGCACCACGTCGCCGCTGCGACTGGCTGCCGCCTATGGCGTGTTCGCCAACCAG
GGCCACCGCATCGAACCCTACCTGATAGACAAGATCACGGATGGCGACGGAAACGTGCTGTTTGCCGCGCAGAACGCAGC
GGAGGTGACGCGTGTGATCTCCGAGCGCAATGCGTTCGTGATGAACAGCCTGCTGCAAAGCGTGGTGGACGAGGGCACCG
GTACCGGCGTGAAACGCTATCTGCGCCGGGACGATGTGGCCGGCAAGACCGGCACCACCAACGATTCGGTAGACGGCTGG
TTCGCCGGCTACGCGGGTGATGTGGTAACCGTGACGTGGATGGGCTTCGATGACAATCGCAGCCTGGGCCGGCACGAGTT
CGGCGCGACCACCGCGCTGCCCATCTGGGCTGCCTACATGGAAGGACGCCTGGCCGGCGCGCCAGAAGCCAGCTTCCCGA
CGCCACCGCAGCTTGCCCGCCTTAATGACGACTGGGCCTACGCGGAGAACGCGGACGGATCCCACGGAATTGCATCGATT
GGATTCCCGCCACCGACTGTCACGCCGGACGTACCTGCCGAGGGCAGCCCTCCCGGCGCACCGACAGCCGCGGCGCCGAC
CCCTGCAGCGCTTGAGGTATCGCCGCAGGTATCGTCCCAACGTCCTGCCGCTACCGGCCTGTGA

Upstream 100 bases:

>100_bases
CGGGAAGTGCCGCATCAGCCGCCAGACCGCACCCAACAGCATCCTCCCTCCGACCATACCGAATCCGGTGTGCCTTTGCC
GCTCCCCGCAGCACGGTAGA

Downstream 100 bases:

>100_bases
CCACGGCCGACATGCCCGCCTACGCGACACCGCTGATCTGGAGCGTCGCGGCACTATCCACGGCGGGCGTGCTGTTCCGG
CCCTTTCGCCTGCCCGAGCC

Product: bifunctional peptidoglycan glycosyl transferase/transpeptidase

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MRAMKKIAVKAAGVVAALGAAGVLLVGFAAVVSLRQLPSVDALRDYRPQVPLRVFTSDDVLIGEFGAEHREFIPIERMPK
LMTEALLAAEDDQFYNHAGVDIPGLVRAALADLGEGYAQGASTITMQVARNFYLSREKTLARKWYEVLLAWQIDRTLPKD
RVLELYMNQVYLGEHAYGFGSAAQTYFGKPLASLSLAETAMLAGLPKAPSTMNPQVNYPRAKRRQEYVLGRMLALGMITQ
DQYREARGAQLVVSRDANGSFSGHAEYVAELARQLAFDRFGDEAYTRGINVYTTVSSVRQTLAYDAVQAGLASYGKRHGS
AKAQVALPQAALVSLDASTGAIEAMVGGASFDTSRFNHATQAQRQPGSTFKPFIYSAALERGISPGTLINDAPLDDPRWQ
PSNDDNRFVGPITVRQALAESRNLPAIRTLRAIGIPYAIDFAGKFGFSTKRLPPYLPLALGTGTTSPLRLAAAYGVFANQ
GHRIEPYLIDKITDGDGNVLFAAQNAAEVTRVISERNAFVMNSLLQSVVDEGTGTGVKRYLRRDDVAGKTGTTNDSVDGW
FAGYAGDVVTVTWMGFDDNRSLGRHEFGATTALPIWAAYMEGRLAGAPEASFPTPPQLARLNDDWAYAENADGSHGIASI
GFPPPTVTPDVPAEGSPPGAPTAAAPTPAALEVSPQVSSQRPAATGL

Sequences:

>Translated_687_residues
MRAMKKIAVKAAGVVAALGAAGVLLVGFAAVVSLRQLPSVDALRDYRPQVPLRVFTSDDVLIGEFGAEHREFIPIERMPK
LMTEALLAAEDDQFYNHAGVDIPGLVRAALADLGEGYAQGASTITMQVARNFYLSREKTLARKWYEVLLAWQIDRTLPKD
RVLELYMNQVYLGEHAYGFGSAAQTYFGKPLASLSLAETAMLAGLPKAPSTMNPQVNYPRAKRRQEYVLGRMLALGMITQ
DQYREARGAQLVVSRDANGSFSGHAEYVAELARQLAFDRFGDEAYTRGINVYTTVSSVRQTLAYDAVQAGLASYGKRHGS
AKAQVALPQAALVSLDASTGAIEAMVGGASFDTSRFNHATQAQRQPGSTFKPFIYSAALERGISPGTLINDAPLDDPRWQ
PSNDDNRFVGPITVRQALAESRNLPAIRTLRAIGIPYAIDFAGKFGFSTKRLPPYLPLALGTGTTSPLRLAAAYGVFANQ
GHRIEPYLIDKITDGDGNVLFAAQNAAEVTRVISERNAFVMNSLLQSVVDEGTGTGVKRYLRRDDVAGKTGTTNDSVDGW
FAGYAGDVVTVTWMGFDDNRSLGRHEFGATTALPIWAAYMEGRLAGAPEASFPTPPQLARLNDDWAYAENADGSHGIASI
GFPPPTVTPDVPAEGSPPGAPTAAAPTPAALEVSPQVSSQRPAATGL
>Mature_687_residues
MRAMKKIAVKAAGVVAALGAAGVLLVGFAAVVSLRQLPSVDALRDYRPQVPLRVFTSDDVLIGEFGAEHREFIPIERMPK
LMTEALLAAEDDQFYNHAGVDIPGLVRAALADLGEGYAQGASTITMQVARNFYLSREKTLARKWYEVLLAWQIDRTLPKD
RVLELYMNQVYLGEHAYGFGSAAQTYFGKPLASLSLAETAMLAGLPKAPSTMNPQVNYPRAKRRQEYVLGRMLALGMITQ
DQYREARGAQLVVSRDANGSFSGHAEYVAELARQLAFDRFGDEAYTRGINVYTTVSSVRQTLAYDAVQAGLASYGKRHGS
AKAQVALPQAALVSLDASTGAIEAMVGGASFDTSRFNHATQAQRQPGSTFKPFIYSAALERGISPGTLINDAPLDDPRWQ
PSNDDNRFVGPITVRQALAESRNLPAIRTLRAIGIPYAIDFAGKFGFSTKRLPPYLPLALGTGTTSPLRLAAAYGVFANQ
GHRIEPYLIDKITDGDGNVLFAAQNAAEVTRVISERNAFVMNSLLQSVVDEGTGTGVKRYLRRDDVAGKTGTTNDSVDGW
FAGYAGDVVTVTWMGFDDNRSLGRHEFGATTALPIWAAYMEGRLAGAPEASFPTPPQLARLNDDWAYAENADGSHGIASI
GFPPPTVTPDVPAEGSPPGAPTAAAPTPAALEVSPQVSSQRPAATGL

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=555, Percent_Identity=34.0540540540541, Blast_Score=262, Evalue=7e-71,
Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=44.299674267101, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1788867, Length=540, Percent_Identity=30, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789601, Length=171, Percent_Identity=32.7485380116959, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 73723; Mature: 73723

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMKKIAVKAAGVVAALGAAGVLLVGFAAVVSLRQLPSVDALRDYRPQVPLRVFTSDDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCE
LIGEFGAEHREFIPIERMPKLMTEALLAAEDDQFYNHAGVDIPGLVRAALADLGEGYAQG
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCC
ASTITMQVARNFYLSREKTLARKWYEVLLAWQIDRTLPKDRVLELYMNQVYLGEHAYGFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCC
SAAQTYFGKPLASLSLAETAMLAGLPKAPSTMNPQVNYPRAKRRQEYVLGRMLALGMITQ
CHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCH
DQYREARGAQLVVSRDANGSFSGHAEYVAELARQLAFDRFGDEAYTRGINVYTTVSSVRQ
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEHHHHHH
TLAYDAVQAGLASYGKRHGSAKAQVALPQAALVSLDASTGAIEAMVGGASFDTSRFNHAT
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCHHEECCCCCCHHHHHHHH
QAQRQPGSTFKPFIYSAALERGISPGTLINDAPLDDPRWQPSNDDNRFVGPITVRQALAE
HHHCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHH
SRNLPAIRTLRAIGIPYAIDFAGKFGFSTKRLPPYLPLALGTGTTSPLRLAAAYGVFANQ
HCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCC
GHRIEPYLIDKITDGDGNVLFAAQNAAEVTRVISERNAFVMNSLLQSVVDEGTGTGVKRY
CCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
LRRDDVAGKTGTTNDSVDGWFAGYAGDVVTVTWMGFDDNRSLGRHEFGATTALPIWAAYM
HHHHCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHH
EGRLAGAPEASFPTPPQLARLNDDWAYAENADGSHGIASIGFPPPTVTPDVPAEGSPPGA
HCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PTAAAPTPAALEVSPQVSSQRPAATGL
CCCCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRAMKKIAVKAAGVVAALGAAGVLLVGFAAVVSLRQLPSVDALRDYRPQVPLRVFTSDDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCE
LIGEFGAEHREFIPIERMPKLMTEALLAAEDDQFYNHAGVDIPGLVRAALADLGEGYAQG
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCC
ASTITMQVARNFYLSREKTLARKWYEVLLAWQIDRTLPKDRVLELYMNQVYLGEHAYGFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCC
SAAQTYFGKPLASLSLAETAMLAGLPKAPSTMNPQVNYPRAKRRQEYVLGRMLALGMITQ
CHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCH
DQYREARGAQLVVSRDANGSFSGHAEYVAELARQLAFDRFGDEAYTRGINVYTTVSSVRQ
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEHHHHHH
TLAYDAVQAGLASYGKRHGSAKAQVALPQAALVSLDASTGAIEAMVGGASFDTSRFNHAT
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCHHEECCCCCCHHHHHHHH
QAQRQPGSTFKPFIYSAALERGISPGTLINDAPLDDPRWQPSNDDNRFVGPITVRQALAE
HHHCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHH
SRNLPAIRTLRAIGIPYAIDFAGKFGFSTKRLPPYLPLALGTGTTSPLRLAAAYGVFANQ
HCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCC
GHRIEPYLIDKITDGDGNVLFAAQNAAEVTRVISERNAFVMNSLLQSVVDEGTGTGVKRY
CCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
LRRDDVAGKTGTTNDSVDGWFAGYAGDVVTVTWMGFDDNRSLGRHEFGATTALPIWAAYM
HHHHCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHH
EGRLAGAPEASFPTPPQLARLNDDWAYAENADGSHGIASIGFPPPTVTPDVPAEGSPPGA
HCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PTAAAPTPAALEVSPQVSSQRPAATGL
CCCCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]