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The map label for this gene is oprM [H]

Identifier: 94312853

GI number: 94312853

Start: 483508

End: 485007

Strand: Direct

Name: oprM [H]

Synonym: Rmet_3925

Alternate gene names: 94312853

Gene position: 483508-485007 (Clockwise)

Preceding gene: 94312852

Following gene: 94312862

Centisome position: 18.74

GC content: 65.6

Gene sequence:

>1500_bases
ATGAGAAAGACGCTACTTTGCCTGGCCGCCGTGGCGGCGCTTAGCGGCTGCAGCCTGATTCCCAACTACCAGCGGCCCGC
CGCGCCGGTCGATGCGACGTTCCCACAGGGTGAGGCCTATCCGAAGACGGCTGCGGTCGATGCGTCGGTGACGGCCGTCG
ACCTTGGCTGGCGCGACTACTTCACCGATCCGCGCTTGCGCGAGCTGATCCAGGTCTCGCTCGACAACAACCGCGATCTG
CGCGTGGCCGTGTTGAACGTGGAGGCTTATCGCGCGCAGTACCGTATCCAGCGTGCCGACCTGTTTCCACAGGTCGACGC
CGCGGCGCAGGGCACGCGGCAGCGCGTGCCGGCGGATCTGTCGGCCACCGGTCAACGTGTGATCAGCAGCCAGTACGGCG
TGTCGCTGGGAACCACGGCTTGGGAACTCGACCTGTTTGGCCGGTTGCGCAGCCTTAGCGAGGCCGCGTTCGAGCAGTAC
CTCGCGGGTGACGAGGCGCGCCGCAGCACACAGATCGCATTGGTGGCCAGCGTTGCCAATGCCTACCTGACGCTGCGCGC
GGACGAGGAGCAACTCAAAGTCACGCGCGACACGCTGACTACGTACGAACGAACGTACAACCTTACGCGCCGCCGTTTCG
AGGAAGGCATCGCCTCGCGGCTGGAGTTGCGCCAGTCGGAAACGCAGGTTGAAACCGCGCGGGCTTCGCTGGCGGCATAC
ACGCGCGCGCTGGCGCAGGACACCAATGCGCTGACGCTGTTGGTGGGTTCGGGCATGCCGGCGGATCTGCCGCAAGGGTT
GGGGCTGGAAGAGAAGCTCGTGGCCGAGGTGCCGGTGGGGCTGCCGTCCGATCTGCTGCAGAATCGTCCGGACATCCTTT
CGGCCGAGCATCGGCTCAAGGCGGCCAACGCGGACATCGGTGCGGCGCGTGCGGCGTTTTTCCCGCGCATCACGCTGACG
GCGTCGGCCGGCACGGCCAGCAGGCAGTTGTCGGACCTGTTCGCGGCCGGTCAGGGCACGTGGCTGTTCTCGCCGCAGAT
CGTGCTGCCGATCTTTACGGCAGGCAGTCTGCAGGCAAGCCTCGACTACGCAAAGATCCAGAAGGACATCAACGTCGCGG
CATACGAGCGGACGATCCAGGGCGCCTTCCGCGAGGTGGCCGATGGACTGGCCGCACGCGGCACGTTCTCGGATCAGGTT
CAGGCACAGGTGCGGCTCGTCCGGGCAAGTCAGGACTACTTCACGCTGGCTGAACATCGGTACAGTACTGGTGTGGATAG
CTACCTGACATTGCTTGATGCGCAACGTCAGCTCTACAACGCGCAACAGACGCTGATCACCGATCGCCTCAACCAGCTCA
CCAGCGAGGTGAACCTGTACAAGGCGCTTGGCGGTGGCTGGACCGATGTCACGCACAGGCCGGAGAATGGCGCGCCAGCT
GATGGGGCCGGAAGTTCGACGACCGGCAGCACGGGCGGCGGCGCAGGGTCGGCTTCGTAG

Upstream 100 bases:

>100_bases
TCGCCATCTTCTGGGTGCCGCTGTTCTTTGTCGTGGTCAGCGCGCTCAAGGAGCGGCGCGGCAAGTCCGATTCGTCCTCC
CTCGAGAAGGGAGCCCAAGC

Downstream 100 bases:

>100_bases
GCCGTAGTCGACTGGAGCACCGGCATCGAAAGGAGGCAGTCTGTGACTGCCTCCTTTTTTATTGCCTGCCGCCAGCCAGC
GGGGCTCGCGCCATCAACGC

Product: multidrug efflux system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MRKTLLCLAAVAALSGCSLIPNYQRPAAPVDATFPQGEAYPKTAAVDASVTAVDLGWRDYFTDPRLRELIQVSLDNNRDL
RVAVLNVEAYRAQYRIQRADLFPQVDAAAQGTRQRVPADLSATGQRVISSQYGVSLGTTAWELDLFGRLRSLSEAAFEQY
LAGDEARRSTQIALVASVANAYLTLRADEEQLKVTRDTLTTYERTYNLTRRRFEEGIASRLELRQSETQVETARASLAAY
TRALAQDTNALTLLVGSGMPADLPQGLGLEEKLVAEVPVGLPSDLLQNRPDILSAEHRLKAANADIGAARAAFFPRITLT
ASAGTASRQLSDLFAAGQGTWLFSPQIVLPIFTAGSLQASLDYAKIQKDINVAAYERTIQGAFREVADGLAARGTFSDQV
QAQVRLVRASQDYFTLAEHRYSTGVDSYLTLLDAQRQLYNAQQTLITDRLNQLTSEVNLYKALGGGWTDVTHRPENGAPA
DGAGSSTTGSTGGGAGSAS

Sequences:

>Translated_499_residues
MRKTLLCLAAVAALSGCSLIPNYQRPAAPVDATFPQGEAYPKTAAVDASVTAVDLGWRDYFTDPRLRELIQVSLDNNRDL
RVAVLNVEAYRAQYRIQRADLFPQVDAAAQGTRQRVPADLSATGQRVISSQYGVSLGTTAWELDLFGRLRSLSEAAFEQY
LAGDEARRSTQIALVASVANAYLTLRADEEQLKVTRDTLTTYERTYNLTRRRFEEGIASRLELRQSETQVETARASLAAY
TRALAQDTNALTLLVGSGMPADLPQGLGLEEKLVAEVPVGLPSDLLQNRPDILSAEHRLKAANADIGAARAAFFPRITLT
ASAGTASRQLSDLFAAGQGTWLFSPQIVLPIFTAGSLQASLDYAKIQKDINVAAYERTIQGAFREVADGLAARGTFSDQV
QAQVRLVRASQDYFTLAEHRYSTGVDSYLTLLDAQRQLYNAQQTLITDRLNQLTSEVNLYKALGGGWTDVTHRPENGAPA
DGAGSSTTGSTGGGAGSAS
>Mature_499_residues
MRKTLLCLAAVAALSGCSLIPNYQRPAAPVDATFPQGEAYPKTAAVDASVTAVDLGWRDYFTDPRLRELIQVSLDNNRDL
RVAVLNVEAYRAQYRIQRADLFPQVDAAAQGTRQRVPADLSATGQRVISSQYGVSLGTTAWELDLFGRLRSLSEAAFEQY
LAGDEARRSTQIALVASVANAYLTLRADEEQLKVTRDTLTTYERTYNLTRRRFEEGIASRLELRQSETQVETARASLAAY
TRALAQDTNALTLLVGSGMPADLPQGLGLEEKLVAEVPVGLPSDLLQNRPDILSAEHRLKAANADIGAARAAFFPRITLT
ASAGTASRQLSDLFAAGQGTWLFSPQIVLPIFTAGSLQASLDYAKIQKDINVAAYERTIQGAFREVADGLAARGTFSDQV
QAQVRLVRASQDYFTLAEHRYSTGVDSYLTLLDAQRQLYNAQQTLITDRLNQLTSEVNLYKALGGGWTDVTHRPENGAPA
DGAGSSTTGSTGGGAGSAS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=41.5948275862069, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI2367348, Length=484, Percent_Identity=24.3801652892562, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53918; Mature: 53918

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTLLCLAAVAALSGCSLIPNYQRPAAPVDATFPQGEAYPKTAAVDASVTAVDLGWRDY
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCHHH
FTDPRLRELIQVSLDNNRDLRVAVLNVEAYRAQYRIQRADLFPQVDAAAQGTRQRVPADL
CCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCCH
SATGQRVISSQYGVSLGTTAWELDLFGRLRSLSEAAFEQYLAGDEARRSTQIALVASVAN
HHHHHHHHHHHCCCEECCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHC
AYLTLRADEEQLKVTRDTLTTYERTYNLTRRRFEEGIASRLELRQSETQVETARASLAAY
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRALAQDTNALTLLVGSGMPADLPQGLGLEEKLVAEVPVGLPSDLLQNRPDILSAEHRLK
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCHHCHHHHHH
AANADIGAARAAFFPRITLTASAGTASRQLSDLFAAGQGTWLFSPQIVLPIFTAGSLQAS
HCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEECCCEEEEEEECCCHHHH
LDYAKIQKDINVAAYERTIQGAFREVADGLAARGTFSDQVQAQVRLVRASQDYFTLAEHR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
YSTGVDSYLTLLDAQRQLYNAQQTLITDRLNQLTSEVNLYKALGGGWTDVTHRPENGAPA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DGAGSSTTGSTGGGAGSAS
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKTLLCLAAVAALSGCSLIPNYQRPAAPVDATFPQGEAYPKTAAVDASVTAVDLGWRDY
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCHHH
FTDPRLRELIQVSLDNNRDLRVAVLNVEAYRAQYRIQRADLFPQVDAAAQGTRQRVPADL
CCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCCH
SATGQRVISSQYGVSLGTTAWELDLFGRLRSLSEAAFEQYLAGDEARRSTQIALVASVAN
HHHHHHHHHHHCCCEECCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHC
AYLTLRADEEQLKVTRDTLTTYERTYNLTRRRFEEGIASRLELRQSETQVETARASLAAY
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRALAQDTNALTLLVGSGMPADLPQGLGLEEKLVAEVPVGLPSDLLQNRPDILSAEHRLK
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCHHCHHHHHH
AANADIGAARAAFFPRITLTASAGTASRQLSDLFAAGQGTWLFSPQIVLPIFTAGSLQAS
HCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEECCCEEEEEEECCCHHHH
LDYAKIQKDINVAAYERTIQGAFREVADGLAARGTFSDQVQAQVRLVRASQDYFTLAEHR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
YSTGVDSYLTLLDAQRQLYNAQQTLITDRLNQLTSEVNLYKALGGGWTDVTHRPENGAPA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DGAGSSTTGSTGGGAGSAS
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]