Click here to switch to the map view.

The map label for this gene is leuS [H]

Identifier: 94311905

GI number: 94311905

Start: 3230761

End: 3233382

Strand: Reverse

Name: leuS [H]

Synonym: Rmet_2973

Alternate gene names: 94311905

Gene position: 3233382-3230761 (Counterclockwise)

Preceding gene: 94311906

Following gene: 94311904

Centisome position: 82.31

GC content: 64.57

Gene sequence:

>2622_bases
ATGCAAGACAAATATCTTCCGTCCGCCGTTGAACAAGCCGCCCAGCAGCACTGGAAAGCGATCGATGCCTACAAGGTGTC
GGAGCACGCGGTCGGGCCCGACGGCAAGGAAAAGTCCAAGTTCTACGCCTGTTCGATGCTGCCCTACCCGTCGGGCAAGC
TCCACATGGGCCACGTGCGCAACTACACGATCAACGACGTGATGGCGCGCTACCTGCGCATGAACGGCCGTAACGTGCTG
ATGCCGATGGGCTGGGATGCGTTCGGGATGCCGGCGGAGAACGCCGCGCTGAACAACGGCGTGGCACCGGCCGCCTGGAC
CTACGACAACATCGCTTACATGAAGAAGCAGATGCAGTCGATGGGCCTGGCGATCGACTGGTCGCGCGAAGTGGCGACCT
GCAGCCCCGAGTACTACCGCTGGAACCAGTGGCTGTTCCTGAAGATGCTGGAAAAGGGCATCGCCTATCGCAAGACCGGT
ACCGTGAACTGGGATCCGGTGGACCAGACCGTGCTGGCCAACGAGCAGGTTATCGACGGCCGTGGCTGGCGTTCGGGCGC
AGTGGTCGAAAAGCGTGAAATCCCGATGTACTACCTGCGCATCACCGATTACGCGCAGGAATTGCTTGGCGACCTCGAAG
GCCTCGGCTGGCCCGAGCGCGTCAAGATCATGCAGCAGAACTGGATCGGCCGCAGTGAAGGTGTGCGATTCGCCTTCCCG
CATGAGATCAAGGGCGCGGATGGCAAGCTGATCAACGACGGCAAGCTCTACGTGTTCACCACGCGTGCCGACACGATTAT
GGGCGTGACGTTCTGCGCCGTGGCCGCCGAGCATCCGCTGGCCACGCACGCCGCCGAAAGCAACCCGGCGCTGGCCGCGT
TCATCGAGGAATGCAAGCACGGTTCCGTCATGGAAGCCGACATGGCGACCATGGAGAAGAAGGGGATGCCGACCGGCCTC
AAGGTCACGCACCCGCTGACCGGCGAACAGGTCGACGTCTGGGTCGGCAACTACGTGCTGATGACCTATGGCGACGGCGC
CGTGATGGGCGTGCCGGCTCATGACGAGCGCGACTTCGCGTTTGCGCTCAAGTACAACCTGCCGATCAAGCAGGTCATCG
ACGTCAAGGGTCAGGCCTATTCGACCGACGCGTGGCTCGAGTGGTACGGCGACAAGGAACACGGCCTCTGCATCCATAGC
GGCAAGTACGACGGTCTCGGCTACAAGGCCGCCGTCGACGCCATCGCCGCCGATCTGGCCGCCAAGGGCCTGGGCGAGAA
GAAGGTGACCTGGCGCCTGCGCGACTGGGGCATCTCGCGCCAGCGTTACTGGGGCACGCCGATTCCGCTGATCCACTGCG
ACAGCTGCGGTGTGGTGCCGGTGCCGGAGAAGGATCTGCCGGTGGTGCTGCCCGAGGACCTCGTGCCGGACGGCACGGGC
AACCCGCTGGCCAAGGACCCGCGTTTCCTGGAGTGCACCTGCCCGTCGTGCGGCAAGCCGGCGCGCCGCGAGACCGATAC
GATGGATACGTTCATCGACTCGTGCTGGTATTACATGCGCTATACGTGCCCCGACGCTGGCACGATGGTCGATGCGCGCA
ACGATTACTGGATGCCGATGGACCAGTACATCGGCGGTATCGAACACGCGATTCTGCACCTGTTGTACGCACGCTTCTGG
ACCAAGGTCATGCGCGACATGGGCCTGGTCAAGTTCGACGAGCCGTTCACGAACCTGCTCACGCAGGGTATGGTGCTCAA
CGAGACGTTCTATCGCGAGGACGCCTCCGGCAAGAAGACCTGGTACAACCCGGCCGATGTGGACGTGCAGACCGACGAGC
GTGGCCGCCCGGCTGGCGCCACGGCGAAGGCCGACGGTCAGCCGGTGGTGATTGGCGGCATCGAGAAGATGTCGAAGTCG
AAGAACAACGGCATCGACCCGCAAGCGCTGATCGACCAGTACGGCGCCGATACCGCACGCCTGTTCGTGATGTTCGCCGC
GCCGCCAGAGCAGCAACTCGAATGGAGCGGTTCGGGCGTGGAGGGCGCGTCGCGCTTCCTGCGCCGTGTGTGGAACTACG
GCTACGCGAACGCCCAGGCGATCCGCGATGGCGCCGGCACCGCGCCGACGGCCGATGACGCCGCGCTGCGCCGCGAGATC
CACACCGTGCTCAAGCAGGCCAACTACGATTACGAGCGCATCCAGTACAACACCGTGGTGTCCGCCACGATGAAGATGCT
GAACGCGCTGGAAGATGCGAAGACCGCTTCGCCGGCCGGGCGCCGTGAAGGCTTCAGCGTGCTGCTGCGCGTGCTGTACC
CGGTGGTGCCGCACATCGCCCACGGGCTGTGGCAGGAACTCGGCTACGCCGCTGAGACGGTCGACATTCTCGACGCCGCA
TGGCCGCAAGTCGACGAAGCCGCGCTGGTGCGCAGCGAGATCGAACTGGTGCTGCAGGTCAACGGCAAGGTGCGTGGCAG
CCTGACGGTGCCGGCCGATGCTGACCGCGCCGCGATCGAGGCCACCGCGGCCGCCAGCGAGATCGTGGCCAAGTTCGCCG
CCGGTGCCGCACCGAAGAAGATCGTGGTGGTGCCGGGCCGTCTCGTCAACGTCGTGCTCTGA

Upstream 100 bases:

>100_bases
ATACGCCACGGTTATAATCGTCTCTTTCGCATTTCCGATCCCCCCGGTCGCCCGGCGCGCCAGTCGCCCGCGGACCGCTG
CCCAGACCGTCGTCCCGACC

Downstream 100 bases:

>100_bases
AGCACGCGGACTGGAATCAGCAGACAGGGATAACGAATGGATCGACCGAACATGGGACGCCGCGGTTTCTTCGCCATCGG
CGCCGCGGCAGCGGCGGCGG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 873; Mature: 873

Protein sequence:

>873_residues
MQDKYLPSAVEQAAQQHWKAIDAYKVSEHAVGPDGKEKSKFYACSMLPYPSGKLHMGHVRNYTINDVMARYLRMNGRNVL
MPMGWDAFGMPAENAALNNGVAPAAWTYDNIAYMKKQMQSMGLAIDWSREVATCSPEYYRWNQWLFLKMLEKGIAYRKTG
TVNWDPVDQTVLANEQVIDGRGWRSGAVVEKREIPMYYLRITDYAQELLGDLEGLGWPERVKIMQQNWIGRSEGVRFAFP
HEIKGADGKLINDGKLYVFTTRADTIMGVTFCAVAAEHPLATHAAESNPALAAFIEECKHGSVMEADMATMEKKGMPTGL
KVTHPLTGEQVDVWVGNYVLMTYGDGAVMGVPAHDERDFAFALKYNLPIKQVIDVKGQAYSTDAWLEWYGDKEHGLCIHS
GKYDGLGYKAAVDAIAADLAAKGLGEKKVTWRLRDWGISRQRYWGTPIPLIHCDSCGVVPVPEKDLPVVLPEDLVPDGTG
NPLAKDPRFLECTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDAGTMVDARNDYWMPMDQYIGGIEHAILHLLYARFW
TKVMRDMGLVKFDEPFTNLLTQGMVLNETFYREDASGKKTWYNPADVDVQTDERGRPAGATAKADGQPVVIGGIEKMSKS
KNNGIDPQALIDQYGADTARLFVMFAAPPEQQLEWSGSGVEGASRFLRRVWNYGYANAQAIRDGAGTAPTADDAALRREI
HTVLKQANYDYERIQYNTVVSATMKMLNALEDAKTASPAGRREGFSVLLRVLYPVVPHIAHGLWQELGYAAETVDILDAA
WPQVDEAALVRSEIELVLQVNGKVRGSLTVPADADRAAIEATAAASEIVAKFAAGAAPKKIVVVPGRLVNVVL

Sequences:

>Translated_873_residues
MQDKYLPSAVEQAAQQHWKAIDAYKVSEHAVGPDGKEKSKFYACSMLPYPSGKLHMGHVRNYTINDVMARYLRMNGRNVL
MPMGWDAFGMPAENAALNNGVAPAAWTYDNIAYMKKQMQSMGLAIDWSREVATCSPEYYRWNQWLFLKMLEKGIAYRKTG
TVNWDPVDQTVLANEQVIDGRGWRSGAVVEKREIPMYYLRITDYAQELLGDLEGLGWPERVKIMQQNWIGRSEGVRFAFP
HEIKGADGKLINDGKLYVFTTRADTIMGVTFCAVAAEHPLATHAAESNPALAAFIEECKHGSVMEADMATMEKKGMPTGL
KVTHPLTGEQVDVWVGNYVLMTYGDGAVMGVPAHDERDFAFALKYNLPIKQVIDVKGQAYSTDAWLEWYGDKEHGLCIHS
GKYDGLGYKAAVDAIAADLAAKGLGEKKVTWRLRDWGISRQRYWGTPIPLIHCDSCGVVPVPEKDLPVVLPEDLVPDGTG
NPLAKDPRFLECTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDAGTMVDARNDYWMPMDQYIGGIEHAILHLLYARFW
TKVMRDMGLVKFDEPFTNLLTQGMVLNETFYREDASGKKTWYNPADVDVQTDERGRPAGATAKADGQPVVIGGIEKMSKS
KNNGIDPQALIDQYGADTARLFVMFAAPPEQQLEWSGSGVEGASRFLRRVWNYGYANAQAIRDGAGTAPTADDAALRREI
HTVLKQANYDYERIQYNTVVSATMKMLNALEDAKTASPAGRREGFSVLLRVLYPVVPHIAHGLWQELGYAAETVDILDAA
WPQVDEAALVRSEIELVLQVNGKVRGSLTVPADADRAAIEATAAASEIVAKFAAGAAPKKIVVVPGRLVNVVL
>Mature_873_residues
MQDKYLPSAVEQAAQQHWKAIDAYKVSEHAVGPDGKEKSKFYACSMLPYPSGKLHMGHVRNYTINDVMARYLRMNGRNVL
MPMGWDAFGMPAENAALNNGVAPAAWTYDNIAYMKKQMQSMGLAIDWSREVATCSPEYYRWNQWLFLKMLEKGIAYRKTG
TVNWDPVDQTVLANEQVIDGRGWRSGAVVEKREIPMYYLRITDYAQELLGDLEGLGWPERVKIMQQNWIGRSEGVRFAFP
HEIKGADGKLINDGKLYVFTTRADTIMGVTFCAVAAEHPLATHAAESNPALAAFIEECKHGSVMEADMATMEKKGMPTGL
KVTHPLTGEQVDVWVGNYVLMTYGDGAVMGVPAHDERDFAFALKYNLPIKQVIDVKGQAYSTDAWLEWYGDKEHGLCIHS
GKYDGLGYKAAVDAIAADLAAKGLGEKKVTWRLRDWGISRQRYWGTPIPLIHCDSCGVVPVPEKDLPVVLPEDLVPDGTG
NPLAKDPRFLECTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDAGTMVDARNDYWMPMDQYIGGIEHAILHLLYARFW
TKVMRDMGLVKFDEPFTNLLTQGMVLNETFYREDASGKKTWYNPADVDVQTDERGRPAGATAKADGQPVVIGGIEKMSKS
KNNGIDPQALIDQYGADTARLFVMFAAPPEQQLEWSGSGVEGASRFLRRVWNYGYANAQAIRDGAGTAPTADDAALRREI
HTVLKQANYDYERIQYNTVVSATMKMLNALEDAKTASPAGRREGFSVLLRVLYPVVPHIAHGLWQELGYAAETVDILDAA
WPQVDEAALVRSEIELVLQVNGKVRGSLTVPADADRAAIEATAAASEIVAKFAAGAAPKKIVVVPGRLVNVVL

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=870, Percent_Identity=35.9770114942529, Blast_Score=518, Evalue=1e-147,
Organism=Escherichia coli, GI1786861, Length=885, Percent_Identity=51.4124293785311, Blast_Score=895, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=934, Percent_Identity=22.9122055674518, Blast_Score=121, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71997517, Length=286, Percent_Identity=40.2097902097902, Blast_Score=228, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71997510, Length=286, Percent_Identity=40.2097902097902, Blast_Score=228, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI212645227, Length=376, Percent_Identity=30.8510638297872, Blast_Score=157, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323414, Length=829, Percent_Identity=41.3751507840772, Blast_Score=567, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6321531, Length=408, Percent_Identity=24.5098039215686, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355409, Length=901, Percent_Identity=34.6281908990011, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281366294, Length=358, Percent_Identity=22.3463687150838, Blast_Score=78, Evalue=3e-14,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96869; Mature: 96869

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKYLPSAVEQAAQQHWKAIDAYKVSEHAVGPDGKEKSKFYACSMLPYPSGKLHMGHVR
CCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCC
NYTINDVMARYLRMNGRNVLMPMGWDAFGMPAENAALNNGVAPAAWTYDNIAYMKKQMQS
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHH
MGLAIDWSREVATCSPEYYRWNQWLFLKMLEKGIAYRKTGTVNWDPVDQTVLANEQVIDG
CCEEEECCCCCCCCCCCHHCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECC
RGWRSGAVVEKREIPMYYLRITDYAQELLGDLEGLGWPERVKIMQQNWIGRSEGVRFAFP
CCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECC
HEIKGADGKLINDGKLYVFTTRADTIMGVTFCAVAAEHPLATHAAESNPALAAFIEECKH
CCCCCCCCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCC
GSVMEADMATMEKKGMPTGLKVTHPLTGEQVDVWVGNYVLMTYGDGAVMGVPAHDERDFA
CCEEHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCEEEEEECCCEEEECCCCCCCCEE
FALKYNLPIKQVIDVKGQAYSTDAWLEWYGDKEHGLCIHSGKYDGLGYKAAVDAIAADLA
EEEEECCCHHHHHCCCCCEECCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
AKGLGEKKVTWRLRDWGISRQRYWGTPIPLIHCDSCGVVPVPEKDLPVVLPEDLVPDGTG
HHCCCCCEEEEEEHHCCCCCHHCCCCCCCEEEECCCCCCCCCCCCCCEECCHHCCCCCCC
NPLAKDPRFLECTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDAGTMVDARNDYWMPM
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCCH
DQYIGGIEHAILHLLYARFWTKVMRDMGLVKFDEPFTNLLTQGMVLNETFYREDASGKKT
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCEEECHHHHCCCCCCCCC
WYNPADVDVQTDERGRPAGATAKADGQPVVIGGIEKMSKSKNNGIDPQALIDQYGADTAR
CCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCHHHHHHHHCCCCEE
LFVMFAAPPEQQLEWSGSGVEGASRFLRRVWNYGYANAQAIRDGAGTAPTADDAALRREI
EEEEEECCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
HTVLKQANYDYERIQYNTVVSATMKMLNALEDAKTASPAGRREGFSVLLRVLYPVVPHIA
HHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
HGLWQELGYAAETVDILDAAWPQVDEAALVRSEIELVLQVNGKVRGSLTVPADADRAAIE
HHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHEEEEEEECCCEEEEEECCCCCCHHHHH
ATAAASEIVAKFAAGAAPKKIVVVPGRLVNVVL
HHHHHHHHHHHHHCCCCCCEEEEECCHHHHEEC
>Mature Secondary Structure
MQDKYLPSAVEQAAQQHWKAIDAYKVSEHAVGPDGKEKSKFYACSMLPYPSGKLHMGHVR
CCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCC
NYTINDVMARYLRMNGRNVLMPMGWDAFGMPAENAALNNGVAPAAWTYDNIAYMKKQMQS
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHH
MGLAIDWSREVATCSPEYYRWNQWLFLKMLEKGIAYRKTGTVNWDPVDQTVLANEQVIDG
CCEEEECCCCCCCCCCCHHCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECC
RGWRSGAVVEKREIPMYYLRITDYAQELLGDLEGLGWPERVKIMQQNWIGRSEGVRFAFP
CCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECC
HEIKGADGKLINDGKLYVFTTRADTIMGVTFCAVAAEHPLATHAAESNPALAAFIEECKH
CCCCCCCCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCC
GSVMEADMATMEKKGMPTGLKVTHPLTGEQVDVWVGNYVLMTYGDGAVMGVPAHDERDFA
CCEEHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCEEEEEECCCEEEECCCCCCCCEE
FALKYNLPIKQVIDVKGQAYSTDAWLEWYGDKEHGLCIHSGKYDGLGYKAAVDAIAADLA
EEEEECCCHHHHHCCCCCEECCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
AKGLGEKKVTWRLRDWGISRQRYWGTPIPLIHCDSCGVVPVPEKDLPVVLPEDLVPDGTG
HHCCCCCEEEEEEHHCCCCCHHCCCCCCCEEEECCCCCCCCCCCCCCEECCHHCCCCCCC
NPLAKDPRFLECTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDAGTMVDARNDYWMPM
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCCH
DQYIGGIEHAILHLLYARFWTKVMRDMGLVKFDEPFTNLLTQGMVLNETFYREDASGKKT
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCEEECHHHHCCCCCCCCC
WYNPADVDVQTDERGRPAGATAKADGQPVVIGGIEKMSKSKNNGIDPQALIDQYGADTAR
CCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCHHHHHHHHCCCCEE
LFVMFAAPPEQQLEWSGSGVEGASRFLRRVWNYGYANAQAIRDGAGTAPTADDAALRREI
EEEEEECCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
HTVLKQANYDYERIQYNTVVSATMKMLNALEDAKTASPAGRREGFSVLLRVLYPVVPHIA
HHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
HGLWQELGYAAETVDILDAAWPQVDEAALVRSEIELVLQVNGKVRGSLTVPADADRAAIE
HHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHEEEEEEECCCEEEEEECCCCCCHHHHH
ATAAASEIVAKFAAGAAPKKIVVVPGRLVNVVL
HHHHHHHHHHHHHCCCCCCEEEEECCHHHHEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA