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The map label for this gene is pilT [H]

Identifier: 94311868

GI number: 94311868

Start: 3191030

End: 3192073

Strand: Reverse

Name: pilT [H]

Synonym: Rmet_2936

Alternate gene names: 94311868

Gene position: 3192073-3191030 (Counterclockwise)

Preceding gene: 94311871

Following gene: 94311867

Centisome position: 81.26

GC content: 61.49

Gene sequence:

>1044_bases
ATGGACATCGCGCAGCTATTGGCTTTTGCGGTCAAGAACAAGGCATCCGATCTCCACCTCTCGGCTGACATGCCGCCGAT
GGTGCGTATTCACGGGGATATGCGCCGCATCAACGTCGCGGCAATGACGCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTTCAGCGCAAGTCTTATGAAGAGCGCCTTGAGGTCGATTTCTCCTTCGAGATCTCTGGTCTGTCCCGT
TTCCGGGTCAACGCCTATACCACCCAGCGCGGCGCGGCCGCGGTGTTCCGGACAATTCCGTCGAAGGTCCTGACGCTCGA
GGACCTGCGCGCGCCCGCCGTGTTCGCCGATCTGTGCATGAAGCCGCGTGGCCTGGTGCTGGTCACCGGGCCCACGGGCT
CGGGCAAGTCGACCACGCTCGCCGCGATGGTCGACCATCGCAACGACAACGACATGGGTCACATCCTCACGGTGGAGGAT
CCAATCGAATTCGTGCATCAGTCCAAGAAGAGTCTGATCAACCAGCGCGAGCTTGGCCCTCACACCCATTCGTTCGCCAA
CGCGCTGCGCTCGGCACTGCGCGAGGACCCGGACGTGGTTCTGGTGGGCGAATTGCGTGACCTGGAAACGATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTTTTCGCCACGCTGCACACGAGTTCGGCCGCCAAGACGATTGACCGTGTG
GTCGACGTGTTCCCGCCGGAAGAAAAGGACATGGTGCGAACCATGCTGTCGGAGTCGCTCGAGGCCGTGATCTCGCAGAC
GCTGCTCAAGACACGCGACGGCAACGGTCGGACGGCGGCACATGAAATCATGATCGCCACGCCTGCGATTCGCCACCTGA
TCCGCGAAAACAAGATCGCGCAGATGTACTCGATGATGCAGACGTCGAGCGGCCTGGGCATGCAGACGCTGGACCAGTGC
CTGAGCGACCTGATCAAGCGGGGCATGATCAACTACAGCGACGCGCGGGCGATTGCGAAAAATCCCGACGCCTTCATGGG
TTAA

Upstream 100 bases:

>100_bases
TCTTCACGGCTTGCAAGTTGGCGGCGATTACAGACATAATCCGGCGAACATACCAAAGAAGTACAACAAAAAGCAGTCTT
TTAGGGTGGGGTCATTATAG

Downstream 100 bases:

>100_bases
GGGGGAAGGGAATGACCATGCTCGACCGCGAATCCGCTGCAAAGTACATCAACGAAATGCTGGGGCTGATGGTCAAGAAC
CGTGGCTCCGATCTGTTCAT

Product: PilT, type IV pilus twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEISGLSR
FRVNAYTTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGMINYSDARAIAKNPDAFMG

Sequences:

>Translated_347_residues
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEISGLSR
FRVNAYTTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGMINYSDARAIAKNPDAFMG
>Mature_347_residues
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEISGLSR
FRVNAYTTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGMINYSDARAIAKNPDAFMG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=330, Percent_Identity=46.3636363636364, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1786296, Length=320, Percent_Identity=32.5, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=33.2378223495702, Blast_Score=131, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38397; Mature: 38397

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHEEEEEEECHHHHHHHHHHHHHHHHH
KSYEERLEVDFSFEISGLSRFRVNAYTTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCM
HHHHHHHCEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHEEHHHHCCHHHHHHHHC
KPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVEDPIEFVHQSKKSLINQRELGP
CCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHEEEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGLGMQTLDQCLSDLIKRGMINYSDARAIAKNPDAFMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHEEEEEEECHHHHHHHHHHHHHHHHH
KSYEERLEVDFSFEISGLSRFRVNAYTTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCM
HHHHHHHCEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHEEHHHHCCHHHHHHHHC
KPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVEDPIEFVHQSKKSLINQRELGP
CCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHEEEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGLGMQTLDQCLSDLIKRGMINYSDARAIAKNPDAFMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]