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The map label for this gene is pilU [H]

Identifier: 94311867

GI number: 94311867

Start: 3189873

End: 3191018

Strand: Reverse

Name: pilU [H]

Synonym: Rmet_2935

Alternate gene names: 94311867

Gene position: 3191018-3189873 (Counterclockwise)

Preceding gene: 94311868

Following gene: 94311866

Centisome position: 81.24

GC content: 61.08

Gene sequence:

>1146_bases
ATGACCATGCTCGACCGCGAATCCGCTGCAAAGTACATCAACGAAATGCTGGGGCTGATGGTCAAGAACCGTGGCTCCGA
TCTGTTCATCACTGCGGAATTTCCTCCGGCCGTCAAGGTCGACGGCAAGATCACGCCGGTTTCCCAGCAGCCGTTGAACC
CGACCCACGCGCTTACCCTGGTGTGCTCGATCATGAACGAGCGCCAGACCAAGGAGTTCGAGGACACCCGCGAATGCAAT
TTCGCGATCGCCGTGCCGGACGTCGGACGTTTCCGTGTCTCGGCCTTCATCCAGCAAGGCCGCGCGGGCATGGTGCTGCG
GACCATCAACACCCGCATTCCATCGGTCGAAGACCTGGACCTGCCGCCGACGCTGCATGAGATCGTGATGTCCAAGCGCG
GTCTGGTGATCGTCACCGGCGCCACTGGCTCGGGCAAGTCGACCTCGCTGGCGGCCATGCTCGACCACCGCAACGCGCAT
TCGTACGGCCACATCATCACGATCGAGGACCCGATCGAATACGTCCACGCGCATCAGAACTGCATCGTCACGCAGCGCGA
GGTGGGGATCGACACCGAGTCGTGGCACGTCGCGCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCG
AAATCCGCGATCGCGACACGATGGAATACGCGATGCAGTACGCGGAAACGGGCCACCTTTGCCTGGCCACGCTGCACGCG
AACAACGCCAATCAGGCGATCGACCGTATCGTCAACTTCTTCCCAGAGGAAAAGCGCCAGCAGCTTCTGATCGACCTGTC
GCTGAACCTGAAGGCGATGATTTCGCAGCGCCTGCTGCCGCGTGCCGGCCGCAAGGGCCGCGTGCCGGCGGTGGAGATCA
TGATCGGCACGCCGCTAGTGGCTGACCTGATCTTCAAGGGCGAGATTCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGC
GAGCAGGGCATGATCTCGTTCGACCAAGCGCTGTTCGAACTCTACGAGACGGAGAAGATCACGTACGAAGACGCGCTCAA
GAACGCTGACTCGCTCAACGACCTGCGCCTGATGATCAAACTCCACAGCAAACGCCATCGCGACAACGATCTCGGCGCCG
GCACCGAACACCTGAACGTGATCTGA

Upstream 100 bases:

>100_bases
AGTGCCTGAGCGACCTGATCAAGCGGGGCATGATCAACTACAGCGACGCGCGGGCGATTGCGAAAAATCCCGACGCCTTC
ATGGGTTAAGGGGGAAGGGA

Downstream 100 bases:

>100_bases
GCGGACGCGGTATTCTCACGGCCTGTACTGCCCCCATACACCGCAGGAGACCACGATGAGCAATGTGTACCAGTTCGAAG
CCAATTCGCTGTCCGGCCAG

Product: PilU, type IV pilus twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTMLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTLVCSIMNERQTKEFEDTRECN
FAIAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAH
SYGHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHA
NNANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSR
EQGMISFDQALFELYETEKITYEDALKNADSLNDLRLMIKLHSKRHRDNDLGAGTEHLNVI

Sequences:

>Translated_381_residues
MTMLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTLVCSIMNERQTKEFEDTRECN
FAIAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAH
SYGHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHA
NNANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSR
EQGMISFDQALFELYETEKITYEDALKNADSLNDLRLMIKLHSKRHRDNDLGAGTEHLNVI
>Mature_380_residues
TMLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTLVCSIMNERQTKEFEDTRECNF
AIAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHS
YGHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHAN
NANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSRE
QGMISFDQALFELYETEKITYEDALKNADSLNDLRLMIKLHSKRHRDNDLGAGTEHLNVI

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=318, Percent_Identity=38.0503144654088, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789723, Length=300, Percent_Identity=31.6666666666667, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786296, Length=294, Percent_Identity=27.5510204081633, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42793; Mature: 42662

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTL
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCEEECCCCCCCCHHHHHHH
VCSIMNERQTKEFEDTRECNFAIAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLD
HHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
LPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSYGHIITIEDPIEYVHAHQN
CCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEECCHHHHHHHCCC
CIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHA
EEEEHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
NNANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLV
CCHHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHH
ADLIFKGEIHELKEVIKKSREQGMISFDQALFELYETEKITYEDALKNADSLNDLRLMIK
HHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
LHSKRHRDNDLGAGTEHLNVI
HHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTL
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCEEECCCCCCCCHHHHHHH
VCSIMNERQTKEFEDTRECNFAIAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLD
HHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
LPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSYGHIITIEDPIEYVHAHQN
CCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEECCHHHHHHHCCC
CIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHA
EEEEHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
NNANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLV
CCHHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHH
ADLIFKGEIHELKEVIKKSREQGMISFDQALFELYETEKITYEDALKNADSLNDLRLMIK
HHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
LHSKRHRDNDLGAGTEHLNVI
HHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]