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The map label for this gene is folK [H]

Identifier: 94311847

GI number: 94311847

Start: 3169721

End: 3170242

Strand: Direct

Name: folK [H]

Synonym: Rmet_2915

Alternate gene names: 94311847

Gene position: 3169721-3170242 (Clockwise)

Preceding gene: 94311846

Following gene: 94311848

Centisome position: 80.69

GC content: 63.6

Gene sequence:

>522_bases
ATGACGCTCGCCTTCATCGGCATCGGCGCCAATCTTGGTGATGCACGCCAGGCAGTAAAAGACGCCATCGTGTGCCTGGC
CCAGCAGGTCGGCATTACGGTGGTCGCCAAGTCGTCGTTCTATCGCACCGCACCAGTCGACGCCACGGGCGCGGACTACT
TCAATGCGGTGGTGAAGATCGAGACGCCGTTCACGGCAGCGCAGCTCCTGCGTATCTGCCACCACATCGAAGACCAGTTC
GGCCGGGAACGGCCGTTCCGGAACGCACCGCGCACGCTCGACCTGGATCTGCTGCTGTTCGGCGCGGAGTCGATCAATAC
CGAAGCGCTGACCGTTCCGCATCCGCGCATCACCGAGCGTGCGTTCGTGCTGGTGCCGCTGGTCGAGCTCGATCCCGCGA
TCGAGATTCCCGGAGTTGGCCCCGCGGCCGGTTTCCTGCCGGACGTGCAAGAACAGCGCATCGAGAAGTTTTCCTCATGC
AAATGCTCGCGCATGAAGGAAGGCGACGGCGCGCGGCCATGA

Upstream 100 bases:

>100_bases
CCGCGGAAATCAGATAAAGTGTCTTCCCGCAGCGAGGCGGATTCGGCAGAAGGAGGTCAGGCCGTCGCACAATCCCGCCG
CGCCCAGCCGGAGGAATAAC

Downstream 100 bases:

>100_bases
GCGCGCCACGCCGCGGCTGACACATGCTCGATCATCTGCGTCGTATTGTCATCGAAGGCCCGGTCGGCTCCGGCAAGGCC
GCGCTGGCCCAACGCCTGGC

Product: 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MTLAFIGIGANLGDARQAVKDAIVCLAQQVGITVVAKSSFYRTAPVDATGADYFNAVVKIETPFTAAQLLRICHHIEDQF
GRERPFRNAPRTLDLDLLLFGAESINTEALTVPHPRITERAFVLVPLVELDPAIEIPGVGPAAGFLPDVQEQRIEKFSSC
KCSRMKEGDGARP

Sequences:

>Translated_173_residues
MTLAFIGIGANLGDARQAVKDAIVCLAQQVGITVVAKSSFYRTAPVDATGADYFNAVVKIETPFTAAQLLRICHHIEDQF
GRERPFRNAPRTLDLDLLLFGAESINTEALTVPHPRITERAFVLVPLVELDPAIEIPGVGPAAGFLPDVQEQRIEKFSSC
KCSRMKEGDGARP
>Mature_172_residues
TLAFIGIGANLGDARQAVKDAIVCLAQQVGITVVAKSSFYRTAPVDATGADYFNAVVKIETPFTAAQLLRICHHIEDQFG
RERPFRNAPRTLDLDLLLFGAESINTEALTVPHPRITERAFVLVPLVELDPAIEIPGVGPAAGFLPDVQEQRIEKFSSCK
CSRMKEGDGARP

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=44.6043165467626, Blast_Score=106, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6324073, Length=136, Percent_Identity=36.7647058823529, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18790; Mature: 18659

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAFIGIGANLGDARQAVKDAIVCLAQQVGITVVAKSSFYRTAPVDATGADYFNAVVKI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCCCCCEEEEEEE
ETPFTAAQLLRICHHIEDQFGRERPFRNAPRTLDLDLLLFGAESINTEALTVPHPRITER
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECEEEEEECCCCCCCCEEECCCCCCCCC
AFVLVPLVELDPAIEIPGVGPAAGFLPDVQEQRIEKFSSCKCSRMKEGDGARP
EEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLAFIGIGANLGDARQAVKDAIVCLAQQVGITVVAKSSFYRTAPVDATGADYFNAVVKI
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCCCCCEEEEEEE
ETPFTAAQLLRICHHIEDQFGRERPFRNAPRTLDLDLLLFGAESINTEALTVPHPRITER
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECEEEEEECCCCCCCCEEECCCCCCCCC
AFVLVPLVELDPAIEIPGVGPAAGFLPDVQEQRIEKFSSCKCSRMKEGDGARP
EEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 2123867 [H]