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The map label for this gene is pcnB [H]

Identifier: 94311846

GI number: 94311846

Start: 3168130

End: 3169719

Strand: Direct

Name: pcnB [H]

Synonym: Rmet_2914

Alternate gene names: 94311846

Gene position: 3168130-3169719 (Clockwise)

Preceding gene: 94311845

Following gene: 94311847

Centisome position: 80.65

GC content: 67.23

Gene sequence:

>1590_bases
GTGATCAAAAAGCTCATCAACCGGCTGCTTGGCAAGCCGGGGCCCAAGCAGCGCCGCACTGGACGCGCCCATGCGGCGCG
CGTCGTCGGCGTTGACGAACACCGAATCGACCCGGCGCTGATCTCGCGCAACGCGGTCAAGGTGACCTCCACCCTGCAGC
AGGCCGGCTACCAGGCCTTCATCGTGGGTGGCGCCGTGCGCGACCTGCTGCTCGGCATCAAGCCGAAGGACTTCGACGTG
GCAACCAATGCCACGCCCGAGCAGGTGCAAGCGCTGTTCCGCCGCTCACGCATCATCGGCCGGCGCTTCCAGATCGTGCA
CGTCACGTTCTACGGCGGCCGCGAGCAGGAGATCATTGAAGTCTCGACCTTCCGCGCGCTGGTCGATGCCGTCGCCAGCG
AGACGCTGCCCGAAGGCCGCCGCCTCAAGCGCGCGGAACTCGACAGCAAGACCCACGCGATCGACGCCAGCGGCCGCGTG
CTGCGCGACAACGTCTGGGGCACGCAGGCCGAGGATGCGGAACGCCGCGACTTCACGATCAACGCGATGTACTACGATCC
GGCCGCGCAGACCGTGCATGACTACCATCACGGCATGGAAGACATCCGCGCGCGCACGCTGCGCATGATCGGCGACCCGG
TCACGCGCTATCGCGAGGACCCCGTGCGCATGTTGCGCGTGGTGCGTTTTGCGGCCAAGACGGGCTTTTCGATCGACGAC
CACACCCGCGAGCCGATCGTCGGTCTGGCCTCGCTGATCCACAATGTGCCCAGCGCGCGCCTGTTCGACGAAATGCTCAA
GCTGCTGATGTCCGGCCATGCGTGGGCCTCGCTGCAGGAACTGCGCAAGGCGGGCCTGCACAAGGGCCTGCTGCCGCTGC
TGGACGTCGCGCTGGAACAACCGATGGGCCAGCGCTTCGTGCAACTCGCCCTCGACAACACCGACCGCCGCGTGCAGGCC
GGCAAGCCGGTTTCTCCGGGCTTCCTGTTCGCATCGCTGCTCTGGCACCACGTGCTGCAGCGCTGGAACAAGCTGCGCGA
GCAAGGCGAGCACCCGATCGCCGCGCTCAACACCGCGATGGATCAGGTGCTGGAAAAGCAGACCGGCCAGCTTGCCATCC
AGCGCCGCTTTGTCACCGACATGCGCGATATCTGGGGAATGCAGCCGCGTTTCGAGAAGCGCGTTGGCCGCATGCCGTTC
CGCCTGCTGGAATCGCCGCGCTTCCGCGCCGGCTACGATTTCCTGCAACTGCGCTGCGAAGCCGGGGAACAGGCGGCCGA
ACTGGGCCAGTGGTGGACCGACTTCCAGAACGCGCCCCCCGATGAGCGCGAAGCGATGATCGAGGCCGCGCGCCCGGCCC
GTTCCAGGGGTGGCAAGGGTGGCCAGGGCGGCCAGGGTGGACAGAGCGGCGCAAACGAACCGGAAGCTGGCGAAGGCGTG
GAAGGTGCGGAAGGCGATGCGCCGGCCCGCAAACGGCGTCGCCGACGCGGTCCGCGGAAATCAGATAAAGTGTCTTCCCG
CAGCGAGGCGGATTCGGCAGAAGGAGGTCAGGCCGTCGCACAATCCCGCCGCGCCCAGCCGGAGGAATAA

Upstream 100 bases:

>100_bases
CTGCTCGAGAAAGTCAGCGAGCCGGTGGCCGCCAACCCGGACGATCGCCTGCGCACCCATGCAGCCTCGGCCGGCTGGCG
CATCATGGATCTGTTCTGAA

Downstream 100 bases:

>100_bases
CATGACGCTCGCCTTCATCGGCATCGGCGCCAATCTTGGTGATGCACGCCAGGCAGTAAAAGACGCCATCGTGTGCCTGG
CCCAGCAGGTCGGCATTACG

Product: poly(A) polymerase I polynucleotide adenylyltransferase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MIKKLINRLLGKPGPKQRRTGRAHAARVVGVDEHRIDPALISRNAVKVTSTLQQAGYQAFIVGGAVRDLLLGIKPKDFDV
ATNATPEQVQALFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVASETLPEGRRLKRAELDSKTHAIDASGRV
LRDNVWGTQAEDAERRDFTINAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPVTRYREDPVRMLRVVRFAAKTGFSIDD
HTREPIVGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQPMGQRFVQLALDNTDRRVQA
GKPVSPGFLFASLLWHHVLQRWNKLREQGEHPIAALNTAMDQVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPF
RLLESPRFRAGYDFLQLRCEAGEQAAELGQWWTDFQNAPPDEREAMIEAARPARSRGGKGGQGGQGGQSGANEPEAGEGV
EGAEGDAPARKRRRRRGPRKSDKVSSRSEADSAEGGQAVAQSRRAQPEE

Sequences:

>Translated_529_residues
MIKKLINRLLGKPGPKQRRTGRAHAARVVGVDEHRIDPALISRNAVKVTSTLQQAGYQAFIVGGAVRDLLLGIKPKDFDV
ATNATPEQVQALFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVASETLPEGRRLKRAELDSKTHAIDASGRV
LRDNVWGTQAEDAERRDFTINAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPVTRYREDPVRMLRVVRFAAKTGFSIDD
HTREPIVGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQPMGQRFVQLALDNTDRRVQA
GKPVSPGFLFASLLWHHVLQRWNKLREQGEHPIAALNTAMDQVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPF
RLLESPRFRAGYDFLQLRCEAGEQAAELGQWWTDFQNAPPDEREAMIEAARPARSRGGKGGQGGQGGQSGANEPEAGEGV
EGAEGDAPARKRRRRRGPRKSDKVSSRSEADSAEGGQAVAQSRRAQPEE
>Mature_529_residues
MIKKLINRLLGKPGPKQRRTGRAHAARVVGVDEHRIDPALISRNAVKVTSTLQQAGYQAFIVGGAVRDLLLGIKPKDFDV
ATNATPEQVQALFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVASETLPEGRRLKRAELDSKTHAIDASGRV
LRDNVWGTQAEDAERRDFTINAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPVTRYREDPVRMLRVVRFAAKTGFSIDD
HTREPIVGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQPMGQRFVQLALDNTDRRVQA
GKPVSPGFLFASLLWHHVLQRWNKLREQGEHPIAALNTAMDQVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPF
RLLESPRFRAGYDFLQLRCEAGEQAAELGQWWTDFQNAPPDEREAMIEAARPARSRGGKGGQGGQGGQSGANEPEAGEGV
EGAEGDAPARKRRRRRGPRKSDKVSSRSEADSAEGGQAVAQSRRAQPEE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=241, Percent_Identity=31.5352697095436, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI87081691, Length=430, Percent_Identity=45.5813953488372, Blast_Score=347, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71995920, Length=346, Percent_Identity=26.0115606936416, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281360112, Length=339, Percent_Identity=27.7286135693215, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357337, Length=339, Percent_Identity=27.7286135693215, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 59272; Mature: 59272

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLINRLLGKPGPKQRRTGRAHAARVVGVDEHRIDPALISRNAVKVTSTLQQAGYQAF
CHHHHHHHHHCCCCCCHHHCCHHHHHHEECCCHHHCCHHHHCCCHHHHHHHHHHCCCEEE
IVGGAVRDLLLGIKPKDFDVATNATPEQVQALFRRSRIIGRRFQIVHVTFYGGREQEIIE
EEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHH
VSTFRALVDAVASETLPEGRRLKRAELDSKTHAIDASGRVLRDNVWGTQAEDAERRDFTI
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHEECCCCCEEECCCCCCCCCCCCCCCEEE
NAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPVTRYREDPVRMLRVVRFAAKTGFSIDD
EEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCC
HTREPIVGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQ
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
PMGQRFVQLALDNTDRRVQAGKPVSPGFLFASLLWHHVLQRWNKLREQGEHPIAALNTAM
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DQVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPFRLLESPRFRAGYDFLQLRCE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHH
AGEQAAELGQWWTDFQNAPPDEREAMIEAARPARSRGGKGGQGGQGGQSGANEPEAGEGV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
EGAEGDAPARKRRRRRGPRKSDKVSSRSEADSAEGGQAVAQSRRAQPEE
CCCCCCCHHHHHHHHCCCCCHHHCCCHHCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKKLINRLLGKPGPKQRRTGRAHAARVVGVDEHRIDPALISRNAVKVTSTLQQAGYQAF
CHHHHHHHHHCCCCCCHHHCCHHHHHHEECCCHHHCCHHHHCCCHHHHHHHHHHCCCEEE
IVGGAVRDLLLGIKPKDFDVATNATPEQVQALFRRSRIIGRRFQIVHVTFYGGREQEIIE
EEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHH
VSTFRALVDAVASETLPEGRRLKRAELDSKTHAIDASGRVLRDNVWGTQAEDAERRDFTI
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHEECCCCCEEECCCCCCCCCCCCCCCEEE
NAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPVTRYREDPVRMLRVVRFAAKTGFSIDD
EEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCC
HTREPIVGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQ
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
PMGQRFVQLALDNTDRRVQAGKPVSPGFLFASLLWHHVLQRWNKLREQGEHPIAALNTAM
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DQVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPFRLLESPRFRAGYDFLQLRCE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHH
AGEQAAELGQWWTDFQNAPPDEREAMIEAARPARSRGGKGGQGGQGGQSGANEPEAGEGV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
EGAEGDAPARKRRRRRGPRKSDKVSSRSEADSAEGGQAVAQSRRAQPEE
CCCCCCCHHHHHHHHCCCCCHHHCCCHHCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]