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The map label for this gene is cysD [H]

Identifier: 94311745

GI number: 94311745

Start: 3060506

End: 3061477

Strand: Reverse

Name: cysD [H]

Synonym: Rmet_2813

Alternate gene names: 94311745

Gene position: 3061477-3060506 (Counterclockwise)

Preceding gene: 94311746

Following gene: 94311744

Centisome position: 77.94

GC content: 64.4

Gene sequence:

>972_bases
ATGGGCATCATGAACGACATCGCAAGCGCAGGCATCCCCGCCAACGTGGAACACCTGCTGCAGGTCCAGAACGACCACCT
CGACCGGCTCGAAGCCGAATCGATCTACATCATCCGCGAGGTGGTGGCCGAGTGCCGCAACCCGGCGCTGCTGTTCTCCG
GCGGCAAGGATTCGATTGTCATGCTGCATCTGGCGCTCAAGGCCTTCCGCTTGGGCGACCGCAAGGTGGAGCTGCCGTTC
CCGCTGGTCCACATCGACACCGGCCACAACTATCCGGAAGTGATCCAGTTCCGCGACCAGCGTGTGGGCGAACTGGGCGC
GCGCCTGGTGGTGGGCCATGTCGAGGATTCGATCAAGCGTGGCACCGTGCGCCTGCGCAAGGAAACCGATTCGCGCAACG
CCGCACAGGCTGTGACGCTGCTGGAAACGATCGAGGCGCACAAGTTCGACGCGCTGATGGGCGGTGCCCGTCGCGATGAA
GAAAAGGCTCGCGCCAAGGAGCGCATCTTCTCGTTCCGCGACGAGTTCGGCCAGTGGGACCCGAAGGCCCAACGCCCGGA
ACTGTGGAGCCTGTACAACGCCCGCATGGCCCAAGGCGAGCAGATGCGCGTGTTTCCGATCTCGAACTGGACCGAACTCG
ACGTGTGGCAGTACATCGCCCGCGAAAACCTGGCCCTGCCGCCGATCTACTACGCGCACGAGCGCGAAGTGGTGCGCAAG
AACGGCCTGCTGGTGCCGGTGACGCCGATTACGCCGAAGCAGGATGGCGATGTGAGCGAAGTGCTGTCGGTCCGCTTCCG
CACCGTGGGCGACATCAGCTGCACGTGCCCGGTGGCTAGCACCGCGGCCACGCCGGTGGAGATCATCGCGGAGACCGCGG
TGACCGAGATCACCGAGCGCGGCGCCACCCGCATGGACGACCAGACGAGCGAAGCGTCGATGGAACGCCGGAAAAAAGAA
GGCTACTTCTAA

Upstream 100 bases:

>100_bases
GCGCCGGACGGTGGTGGTGGGAGAGCAAGGACTCGAAAGAGTGCGGGCTCCACGAACAGAACATCAAGCACTGAACAGAA
TATTTGAGGCCGAGAACGAC

Downstream 100 bases:

>100_bases
ATCGACGCAGCAGAGGAATACGACAATGTCTCACCAATCCCAACATCAAGGCCTGCTGCGTTTCATCACCGCGGGCTCCG
TGGACGACGGCAAGAGCACG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MGIMNDIASAGIPANVEHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKVELPF
PLVHIDTGHNYPEVIQFRDQRVGELGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIEAHKFDALMGGARRDE
EKARAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRK
NGLLVPVTPITPKQDGDVSEVLSVRFRTVGDISCTCPVASTAATPVEIIAETAVTEITERGATRMDDQTSEASMERRKKE
GYF

Sequences:

>Translated_323_residues
MGIMNDIASAGIPANVEHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKVELPF
PLVHIDTGHNYPEVIQFRDQRVGELGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIEAHKFDALMGGARRDE
EKARAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRK
NGLLVPVTPITPKQDGDVSEVLSVRFRTVGDISCTCPVASTAATPVEIIAETAVTEITERGATRMDDQTSEASMERRKKE
GYF
>Mature_322_residues
GIMNDIASAGIPANVEHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKVELPFP
LVHIDTGHNYPEVIQFRDQRVGELGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIEAHKFDALMGGARRDEE
KARAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRKN
GLLVPVTPITPKQDGDVSEVLSVRFRTVGDISCTCPVASTAATPVEIIAETAVTEITERGATRMDDQTSEASMERRKKEG
YF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=305, Percent_Identity=46.8852459016393, Blast_Score=261, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36495; Mature: 36364

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIMNDIASAGIPANVEHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIV
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEE
MLHLALKAFRLGDRKVELPFPLVHIDTGHNYPEVIQFRDQRVGELGARLVVGHVEDSIKR
HHHHHHHHHHCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHHH
GTVRLRKETDSRNAAQAVTLLETIEAHKFDALMGGARRDEEKARAKERIFSFRDEFGQWD
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRK
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
NGLLVPVTPITPKQDGDVSEVLSVRFRTVGDISCTCPVASTAATPVEIIAETAVTEITER
CCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHC
GATRMDDQTSEASMERRKKEGYF
CCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIMNDIASAGIPANVEHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIV
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEE
MLHLALKAFRLGDRKVELPFPLVHIDTGHNYPEVIQFRDQRVGELGARLVVGHVEDSIKR
HHHHHHHHHHCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHHH
GTVRLRKETDSRNAAQAVTLLETIEAHKFDALMGGARRDEEKARAKERIFSFRDEFGQWD
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRK
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
NGLLVPVTPITPKQDGDVSEVLSVRFRTVGDISCTCPVASTAATPVEIIAETAVTEITER
CCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHC
GATRMDDQTSEASMERRKKEGYF
CCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA