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The map label for this gene is cysH [H]

Identifier: 94311746

GI number: 94311746

Start: 3061504

End: 3062304

Strand: Reverse

Name: cysH [H]

Synonym: Rmet_2814

Alternate gene names: 94311746

Gene position: 3062304-3061504 (Counterclockwise)

Preceding gene: 94311747

Following gene: 94311745

Centisome position: 77.96

GC content: 66.04

Gene sequence:

>801_bases
ATGAGCTCCGTGATCTCCGAGATCCCAGTCGTCGACGGTTCGGTGTCGTCGCTGCGCCCCCCAGCGCTGTGGACGAAGCC
GGCGTACGCCGGCACGATCGAGGCGCTCGAAGTGAAGGAACGTGAACTGGCCGAACGCCTGTCCGGAATCGCGGCGCGTT
TCTTCCGCGCGCGCTTCGCCACGAGCCTGGCCGCCGAAGACATGGTGCTGACCGACGCGATCCTGCGCGGCAGCCCGGCC
GTGCGCGCCGGCATCCGCGTGTTCACGCTGAACACCGGCCGCCTGCACGCCGAAACGCTTGCTGTGCTGGACAAGGTCGA
GACGCACTACGGCTACACGATCGAGCAGTTCACGCCCGACCAGGAAGCCGTGGAGAACTACCTGAAGAAGAACGGCCTGA
ATGCCTTCTACGACAGCGTGGACCTGCGCAAGAGCTGCTGCGGCATCCGCAAGGTGGAACCGCTCAATCGCGCGCTTTCG
CACGCCGATGCGTGGATGACGGGCCAGCGCCGCGAGCAGGCCGTGACGCGCGCCGAACTGCCGTTCGAGGAAACGGACGA
GTCGCGCGGCATTCCCAAGTTCAATCCGCTGGCCGACTGGACCGAGGCCGAAGTGTGGGCGTACCTGTCGCGCCACAACG
TCCCGGTCAACGCGCTGCACGAGAAGGGCTATCCGAGCATCGGTTGCGAGCCCTGTACGCGGGCGGTCAAGGCCGGCGAG
GATGTACGCGCCGGACGGTGGTGGTGGGAGAGCAAGGACTCGAAAGAGTGCGGGCTCCACGAACAGAACATCAAGCACTG
A

Upstream 100 bases:

>100_bases
AGGGCTTCACCGAGTTCACGGTGGCCTACCAGGCTTCGGTGGACGAGCCGCTGCCGCTGTTCCGCCGCGCACGTGCCGAC
GTGACCGCCCGGGGCGTGGC

Downstream 100 bases:

>100_bases
ACAGAATATTTGAGGCCGAGAACGACATGGGCATCATGAACGACATCGCAAGCGCAGGCATCCCCGCCAACGTGGAACAC
CTGCTGCAGGTCCAGAACGA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSSVISEIPVVDGSVSSLRPPALWTKPAYAGTIEALEVKERELAERLSGIAARFFRARFATSLAAEDMVLTDAILRGSPA
VRAGIRVFTLNTGRLHAETLAVLDKVETHYGYTIEQFTPDQEAVENYLKKNGLNAFYDSVDLRKSCCGIRKVEPLNRALS
HADAWMTGQRREQAVTRAELPFEETDESRGIPKFNPLADWTEAEVWAYLSRHNVPVNALHEKGYPSIGCEPCTRAVKAGE
DVRAGRWWWESKDSKECGLHEQNIKH

Sequences:

>Translated_266_residues
MSSVISEIPVVDGSVSSLRPPALWTKPAYAGTIEALEVKERELAERLSGIAARFFRARFATSLAAEDMVLTDAILRGSPA
VRAGIRVFTLNTGRLHAETLAVLDKVETHYGYTIEQFTPDQEAVENYLKKNGLNAFYDSVDLRKSCCGIRKVEPLNRALS
HADAWMTGQRREQAVTRAELPFEETDESRGIPKFNPLADWTEAEVWAYLSRHNVPVNALHEKGYPSIGCEPCTRAVKAGE
DVRAGRWWWESKDSKECGLHEQNIKH
>Mature_265_residues
SSVISEIPVVDGSVSSLRPPALWTKPAYAGTIEALEVKERELAERLSGIAARFFRARFATSLAAEDMVLTDAILRGSPAV
RAGIRVFTLNTGRLHAETLAVLDKVETHYGYTIEQFTPDQEAVENYLKKNGLNAFYDSVDLRKSCCGIRKVEPLNRALSH
ADAWMTGQRREQAVTRAELPFEETDESRGIPKFNPLADWTEAEVWAYLSRHNVPVNALHEKGYPSIGCEPCTRAVKAGED
VRAGRWWWESKDSKECGLHEQNIKH

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=188, Percent_Identity=31.3829787234043, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325425, Length=209, Percent_Identity=34.4497607655502, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29785; Mature: 29654

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVISEIPVVDGSVSSLRPPALWTKPAYAGTIEALEVKERELAERLSGIAARFFRARFA
CCCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLAAEDMVLTDAILRGSPAVRAGIRVFTLNTGRLHAETLAVLDKVETHYGYTIEQFTPD
HHHHHHHHHHHHHHHHCCCHHHHCEEEEEEECCCHHHHHHHHHHHHHHHCCEEHHHCCCC
QEAVENYLKKNGLNAFYDSVDLRKSCCGIRKVEPLNRALSHADAWMTGQRREQAVTRAEL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCC
PFEETDESRGIPKFNPLADWTEAEVWAYLSRHNVPVNALHEKGYPSIGCEPCTRAVKAGE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHCCC
DVRAGRWWWESKDSKECGLHEQNIKH
CCCCCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SSVISEIPVVDGSVSSLRPPALWTKPAYAGTIEALEVKERELAERLSGIAARFFRARFA
CCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLAAEDMVLTDAILRGSPAVRAGIRVFTLNTGRLHAETLAVLDKVETHYGYTIEQFTPD
HHHHHHHHHHHHHHHHCCCHHHHCEEEEEEECCCHHHHHHHHHHHHHHHCCEEHHHCCCC
QEAVENYLKKNGLNAFYDSVDLRKSCCGIRKVEPLNRALSHADAWMTGQRREQAVTRAEL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCC
PFEETDESRGIPKFNPLADWTEAEVWAYLSRHNVPVNALHEKGYPSIGCEPCTRAVKAGE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHCCC
DVRAGRWWWESKDSKECGLHEQNIKH
CCCCCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]