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The map label for this gene is wcaJ [H]

Identifier: 94311650

GI number: 94311650

Start: 2952004

End: 2953329

Strand: Direct

Name: wcaJ [H]

Synonym: Rmet_2718

Alternate gene names: 94311650

Gene position: 2952004-2953329 (Clockwise)

Preceding gene: 94311649

Following gene: 94311651

Centisome position: 75.15

GC content: 64.18

Gene sequence:

>1326_bases
ATGAGCAACAAGCAAGCCCGCCTCACCCATTCCCAGCGAAGCCCGGCCGCGAACACGCGCGGCTCCGAAACCGTGCGTCG
CGCCAGCCGGATGCTGTCCTGGGCCTTGGTCGGCCTGGTGCTGTTCGGCATCAATACCGTGGCGGCAGAATACCTGCACC
ACTCGGGTATCGTGACCTACGTGTTCACGCGCACCCTGCTGTGGGCGATCGTCCCCTACCTGTGTACGTTCGTGCTGCTG
CATCGGTCGTTGCATCTTCCGGCAATCGAAGGCAACAGCCTTGTCGGCATGTCGGCGACGCTGCCTTTCGGCCTGCTGCT
GATCGTCTTCGCCGCGTTCCATATCGAGTATTCCCGCGGCGCCCTGCTGGTCGGCTATATCACGACGCTGGTGTGGAGCG
GCATCGGCTACCGGCGCTTCGTGCAGAACTACGTGCCGGTGTTCGGCTATACCGATCCGCGCACGCTCGAACAGCTTGAA
GAAGTCCTGGCCATGCCCGGCGCCACGCCACCTACCCCCACCCGTTTCGAGTTCATCCATGCCATCGAGGATGCCGTCCA
TTGCGATGGCCTGATGCTGGACCGATCCGCCACGGCGGACCCTGAGCGGACGCGTGCGCTGGCGCGCCTGAAGCTCAGCC
ATGTGCGCATGTATTCGGTGGAACGGGTTGGCGAGATGCTCACGGGCCGCGTTGGCCTCGATCACATCGACGAGAATTTC
CTCGACGACTATGCGCGCCACTATCTCTATGGATACGTGAAGCGCCTGGTGGACATCGCCGCCGTGCTTTGCCTGGCGCC
CCTGGCGTTGCCGCTCTCCGCGCTGACGGCGCTGGCGATCCGGCTGGAATCCACGGGGCCGGTCCTGTTCCGGCAGGCGC
GTGTGGGCCGCTTCGGCGAACCATTCACGATGCTCAAATTCCGCAGCATGAGCGTGCAGCCCGACGCCACCGCCCAGTTC
GCGCAACGCCACGATCCGCGCGTGACACGCGTGGGCCGCGTGATCCGCAAGTACCGTCTGGACGAACTGCCGCAGCTCTG
GAACGTGCTGACCGGCGACATGAGCCTGATTGGGCCACGCCCGGAACAGGTGCCAATGGTCGACATGTTCGCCGATACCA
TTGCCTACTACCCTTACCGCCATCTGGTTCGCCCCGGCCTGTCGGGATGGGCCCAAGTTCAGCAGGGCTACGTCGGCACG
CACGCGGAAACGGTCACCAAGCTCAGCTACGACCTCTACTACGTCAAGCACTGCTCCTTCGCACTCGACCTGCTGATTGC
CGTCAAAACCGTGCGCACGCTGCTGACCGGGTACGGAGCGCGTTGA

Upstream 100 bases:

>100_bases
GCGTTCGGCGTTGACACCATGGCCGACGCCACCTGGCAGGTTTACCAGCGTGTGCTCGGGCCTGGGCAGACAGCCGCGAT
GCCGGCCGGGGGAGCCTGAG

Downstream 100 bases:

>100_bases
TGGCAGGCACGACCGGGGATACCTCACCCACCGGCGCTCCGATGCGCATCCTGCTGGTTACCACCGGCCTGAAGATGGGC
GGCGCCGAGCACCAGGTAGT

Product: putative sugar transferase involved in lipopolysaccharide synthesis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSNKQARLTHSQRSPAANTRGSETVRRASRMLSWALVGLVLFGINTVAAEYLHHSGIVTYVFTRTLLWAIVPYLCTFVLL
HRSLHLPAIEGNSLVGMSATLPFGLLLIVFAAFHIEYSRGALLVGYITTLVWSGIGYRRFVQNYVPVFGYTDPRTLEQLE
EVLAMPGATPPTPTRFEFIHAIEDAVHCDGLMLDRSATADPERTRALARLKLSHVRMYSVERVGEMLTGRVGLDHIDENF
LDDYARHYLYGYVKRLVDIAAVLCLAPLALPLSALTALAIRLESTGPVLFRQARVGRFGEPFTMLKFRSMSVQPDATAQF
AQRHDPRVTRVGRVIRKYRLDELPQLWNVLTGDMSLIGPRPEQVPMVDMFADTIAYYPYRHLVRPGLSGWAQVQQGYVGT
HAETVTKLSYDLYYVKHCSFALDLLIAVKTVRTLLTGYGAR

Sequences:

>Translated_441_residues
MSNKQARLTHSQRSPAANTRGSETVRRASRMLSWALVGLVLFGINTVAAEYLHHSGIVTYVFTRTLLWAIVPYLCTFVLL
HRSLHLPAIEGNSLVGMSATLPFGLLLIVFAAFHIEYSRGALLVGYITTLVWSGIGYRRFVQNYVPVFGYTDPRTLEQLE
EVLAMPGATPPTPTRFEFIHAIEDAVHCDGLMLDRSATADPERTRALARLKLSHVRMYSVERVGEMLTGRVGLDHIDENF
LDDYARHYLYGYVKRLVDIAAVLCLAPLALPLSALTALAIRLESTGPVLFRQARVGRFGEPFTMLKFRSMSVQPDATAQF
AQRHDPRVTRVGRVIRKYRLDELPQLWNVLTGDMSLIGPRPEQVPMVDMFADTIAYYPYRHLVRPGLSGWAQVQQGYVGT
HAETVTKLSYDLYYVKHCSFALDLLIAVKTVRTLLTGYGAR
>Mature_440_residues
SNKQARLTHSQRSPAANTRGSETVRRASRMLSWALVGLVLFGINTVAAEYLHHSGIVTYVFTRTLLWAIVPYLCTFVLLH
RSLHLPAIEGNSLVGMSATLPFGLLLIVFAAFHIEYSRGALLVGYITTLVWSGIGYRRFVQNYVPVFGYTDPRTLEQLEE
VLAMPGATPPTPTRFEFIHAIEDAVHCDGLMLDRSATADPERTRALARLKLSHVRMYSVERVGEMLTGRVGLDHIDENFL
DDYARHYLYGYVKRLVDIAAVLCLAPLALPLSALTALAIRLESTGPVLFRQARVGRFGEPFTMLKFRSMSVQPDATAQFA
QRHDPRVTRVGRVIRKYRLDELPQLWNVLTGDMSLIGPRPEQVPMVDMFADTIAYYPYRHLVRPGLSGWAQVQQGYVGTH
AETVTKLSYDLYYVKHCSFALDLLIAVKTVRTLLTGYGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=186, Percent_Identity=42.4731182795699, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 49532; Mature: 49401

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKQARLTHSQRSPAANTRGSETVRRASRMLSWALVGLVLFGINTVAAEYLHHSGIVTY
CCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VFTRTLLWAIVPYLCTFVLLHRSLHLPAIEGNSLVGMSATLPFGLLLIVFAAFHIEYSRG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCC
ALLVGYITTLVWSGIGYRRFVQNYVPVFGYTDPRTLEQLEEVLAMPGATPPTPTRFEFIH
CHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
AIEDAVHCDGLMLDRSATADPERTRALARLKLSHVRMYSVERVGEMLTGRVGLDHIDENF
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LDDYARHYLYGYVKRLVDIAAVLCLAPLALPLSALTALAIRLESTGPVLFRQARVGRFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
PFTMLKFRSMSVQPDATAQFAQRHDPRVTRVGRVIRKYRLDELPQLWNVLTGDMSLIGPR
CHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
PEQVPMVDMFADTIAYYPYRHLVRPGLSGWAQVQQGYVGTHAETVTKLSYDLYYVKHCSF
CCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ALDLLIAVKTVRTLLTGYGAR
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNKQARLTHSQRSPAANTRGSETVRRASRMLSWALVGLVLFGINTVAAEYLHHSGIVTY
CCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VFTRTLLWAIVPYLCTFVLLHRSLHLPAIEGNSLVGMSATLPFGLLLIVFAAFHIEYSRG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCC
ALLVGYITTLVWSGIGYRRFVQNYVPVFGYTDPRTLEQLEEVLAMPGATPPTPTRFEFIH
CHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
AIEDAVHCDGLMLDRSATADPERTRALARLKLSHVRMYSVERVGEMLTGRVGLDHIDENF
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LDDYARHYLYGYVKRLVDIAAVLCLAPLALPLSALTALAIRLESTGPVLFRQARVGRFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
PFTMLKFRSMSVQPDATAQFAQRHDPRVTRVGRVIRKYRLDELPQLWNVLTGDMSLIGPR
CHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
PEQVPMVDMFADTIAYYPYRHLVRPGLSGWAQVQQGYVGTHAETVTKLSYDLYYVKHCSF
CCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ALDLLIAVKTVRTLLTGYGAR
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]