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The map label for this gene is ribE

Identifier: 94311626

GI number: 94311626

Start: 2925573

End: 2926082

Strand: Direct

Name: ribE

Synonym: Rmet_2694

Alternate gene names: 94311626

Gene position: 2925573-2926082 (Clockwise)

Preceding gene: 94311625

Following gene: 94311627

Centisome position: 74.48

GC content: 64.31

Gene sequence:

>510_bases
ATGGATCACGGCTTCTACGAGAGCAACCTCGACGGTGAAGGCCTGCGTATCGGCATCGTGCAGGCACGCTTCAACGAGCC
CGTCTGCGACGAACTGCGCGAGGCCTGCATGGCAGAACTGAAAAAGCTGGGCGTCGAAGGCGAAGACACGCTGCTGGTGA
CCGTGCCGGGCGCACTGGAAGTGCCGCTGGCGCTGCAGAAAATGGCCGAAAGCGGCCAGTTCGACGCGCTGGTCGCGCTG
GGCGCGGTGGTGCGTGGCGAAACGTATCACTTCGAGTTGGTCTCGAATGAATCCGGCGCCGGTATCACCCGCGTGGGCCT
CGATTTCAACGTGCCCATCGCCAACGGCATCCTGACCGTCGACACCGACGCCCAGGCCCATGCCCGCACCCGCGAGAAGG
GCCGCGATTGCGCCCGCGCCGCAGTGGAAATGGCCAACCTGGTGGTCGCGCTCGATTCGCTGCGCGAGCATGGCGCAGAA
GACGCAGAAGACGAGGAAGATGATGAGTGA

Upstream 100 bases:

>100_bases
CGGCGAGTCGAATCGCGGCTGACCGGGAACGGATCGTACCGATTGACCCGGCAGCGGCGCATCCGCGCGGCTGCCTGACC
GAATCACTGGAGAAATAACA

Downstream 100 bases:

>100_bases
CGCGCCCGACACCGGCGACAAGGGCGAGGCAGCCGGCAAGCCGGCTGGCGCCAAGCCCCGCACCGAGCCGAAGGCACCGC
CGAAGAGCGCGCGTCGCCGT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MDHGFYESNLDGEGLRIGIVQARFNEPVCDELREACMAELKKLGVEGEDTLLVTVPGALEVPLALQKMAESGQFDALVAL
GAVVRGETYHFELVSNESGAGITRVGLDFNVPIANGILTVDTDAQAHARTREKGRDCARAAVEMANLVVALDSLREHGAE
DAEDEEDDE

Sequences:

>Translated_169_residues
MDHGFYESNLDGEGLRIGIVQARFNEPVCDELREACMAELKKLGVEGEDTLLVTVPGALEVPLALQKMAESGQFDALVAL
GAVVRGETYHFELVSNESGAGITRVGLDFNVPIANGILTVDTDAQAHARTREKGRDCARAAVEMANLVVALDSLREHGAE
DAEDEEDDE
>Mature_169_residues
MDHGFYESNLDGEGLRIGIVQARFNEPVCDELREACMAELKKLGVEGEDTLLVTVPGALEVPLALQKMAESGQFDALVAL
GAVVRGETYHFELVSNESGAGITRVGLDFNVPIANGILTVDTDAQAHARTREKGRDCARAAVEMANLVVALDSLREHGAE
DAEDEEDDE

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=152, Percent_Identity=39.4736842105263, Blast_Score=104, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324429, Length=148, Percent_Identity=31.7567567567568, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_RALME (Q1LJV9)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584836.1
- ProteinModelPortal:   Q1LJV9
- SMR:   Q1LJV9
- STRING:   Q1LJV9
- GeneID:   4039519
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2694
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   Q1LJV9
- ProtClustDB:   PRK00061
- BioCyc:   RMET266264:RMET_2694-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 18183; Mature: 18183

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHGFYESNLDGEGLRIGIVQARFNEPVCDELREACMAELKKLGVEGEDTLLVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
VPLALQKMAESGQFDALVALGAVVRGETYHFELVSNESGAGITRVGLDFNVPIANGILTV
CCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCCEEEE
DTDAQAHARTREKGRDCARAAVEMANLVVALDSLREHGAEDAEDEEDDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDHGFYESNLDGEGLRIGIVQARFNEPVCDELREACMAELKKLGVEGEDTLLVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
VPLALQKMAESGQFDALVALGAVVRGETYHFELVSNESGAGITRVGLDFNVPIANGILTV
CCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCCEEEE
DTDAQAHARTREKGRDCARAAVEMANLVVALDSLREHGAEDAEDEEDDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA