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The map label for this gene is gluQ

Identifier: 94311517

GI number: 94311517

Start: 2805181

End: 2806113

Strand: Direct

Name: gluQ

Synonym: Rmet_2581

Alternate gene names: 94311517

Gene position: 2805181-2806113 (Clockwise)

Preceding gene: 94311516

Following gene: 94311519

Centisome position: 71.41

GC content: 67.1

Gene sequence:

>933_bases
ATGTCACAACGCTACCGGGGCCGCTTCGCGCCCTCCCCCACTGGCCCGCTCCATCTGGGCTCCCTGGTTACCGCGCTGGC
GAGTTGGCTGGACGCGCGCGCCCATGGCGGCGACTGGCTGGTGCGGATCGAGGACATCGACTACCCGCGCTGCGTCAGGG
ACGCCGACAATGACATCCTGCGCACACTCGACGCACTTGGCCTGCACCCCGATGAACCGCCGGTCTGGCAAAGCCGTCGC
GAAGGCCTCTACGCGGAGGCGCTGCGCCAGCTCGACGCCGCCGGCTATCTCTATCCGTGCGGCTGCACGCGCAAGGAAAT
CGCCGATTCGCTCGTGCACGTGCGTGAACGGCATCAGACACTCGGCTACCCGGGCACTTGCCGCAATGGCCTGCATGGCA
AGCTGCCACGTGCCTGGCGCGTGCGCGTGCCAGATGGCCCGGCGGCAACGATCTGCTTTGACGACCGCTGGCAAGGCCGC
CAATGCCAGAACCTGGAAACGGAACTCGGGGATTTCGTACTGAGACGGGCCGATGGGCTATGGGCCTATCAGCTTGCGGT
TGTGGTCGATGACGCCGCGCAGGGCATCACGCATATCGTGCGTGGAGCGGACCTGCTGGATTCGACACCGCGACAGATCC
ACCTCGAGCATTTGCTGGGCTTGCCGACGCCGGACTATCTGCACGTGCCGGTGGTGGTCAACGCAGTCGGAGAGAAATTG
AGCAAGCAGAGCGGCGCGCAAGCGCTCGATGCTTCGGCGCCGCTCGATGCGCTCCGCACTGCGGGAACGCATCTTGGCAT
CGTGAACCAGGAGACGACCGTGGCCGCCTGGCTGGCGACCGCTACCGGTCAGTGGCTGGAACGGCTACGCGCCGTTCAGG
ACTTGCGCGGCACGCCGCCGAGCAGCGCGGCCACGGGGCGCTTGGGCGCCTGA

Upstream 100 bases:

>100_bases
GGCTGGCACAGGCGCTATTGCTTCAGCCGCCGCACTCTCCGCTAGATCAGACCGACTGAAACAGACCGACTGCCACAGAG
CGACTGAATCCCGCAGTTCC

Downstream 100 bases:

>100_bases
CGTCCACGCGTCAGCGCGGCGGCCAGTTGTTCCTCGGTCTTCGGTGCCGGCGTGTAGCTGCCCGACGGCTCGTACGGACG
CGAGAAGAACGGATCGTCCG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSQRYRGRFAPSPTGPLHLGSLVTALASWLDARAHGGDWLVRIEDIDYPRCVRDADNDILRTLDALGLHPDEPPVWQSRR
EGLYAEALRQLDAAGYLYPCGCTRKEIADSLVHVRERHQTLGYPGTCRNGLHGKLPRAWRVRVPDGPAATICFDDRWQGR
QCQNLETELGDFVLRRADGLWAYQLAVVVDDAAQGITHIVRGADLLDSTPRQIHLEHLLGLPTPDYLHVPVVVNAVGEKL
SKQSGAQALDASAPLDALRTAGTHLGIVNQETTVAAWLATATGQWLERLRAVQDLRGTPPSSAATGRLGA

Sequences:

>Translated_310_residues
MSQRYRGRFAPSPTGPLHLGSLVTALASWLDARAHGGDWLVRIEDIDYPRCVRDADNDILRTLDALGLHPDEPPVWQSRR
EGLYAEALRQLDAAGYLYPCGCTRKEIADSLVHVRERHQTLGYPGTCRNGLHGKLPRAWRVRVPDGPAATICFDDRWQGR
QCQNLETELGDFVLRRADGLWAYQLAVVVDDAAQGITHIVRGADLLDSTPRQIHLEHLLGLPTPDYLHVPVVVNAVGEKL
SKQSGAQALDASAPLDALRTAGTHLGIVNQETTVAAWLATATGQWLERLRAVQDLRGTPPSSAATGRLGA
>Mature_309_residues
SQRYRGRFAPSPTGPLHLGSLVTALASWLDARAHGGDWLVRIEDIDYPRCVRDADNDILRTLDALGLHPDEPPVWQSRRE
GLYAEALRQLDAAGYLYPCGCTRKEIADSLVHVRERHQTLGYPGTCRNGLHGKLPRAWRVRVPDGPAATICFDDRWQGRQ
CQNLETELGDFVLRRADGLWAYQLAVVVDDAAQGITHIVRGADLLDSTPRQIHLEHLLGLPTPDYLHVPVVVNAVGEKLS
KQSGAQALDASAPLDALRTAGTHLGIVNQETTVAAWLATATGQWLERLRAVQDLRGTPPSSAATGRLGA

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=264, Percent_Identity=35.9848484848485, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI226510925, Length=295, Percent_Identity=45.4237288135593, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788743, Length=251, Percent_Identity=37.8486055776892, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71993394, Length=286, Percent_Identity=32.5174825174825, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324540, Length=265, Percent_Identity=33.2075471698113, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24665321, Length=256, Percent_Identity=35.15625, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_RALME (Q1LK68)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584727.1
- ProteinModelPortal:   Q1LK68
- SMR:   Q1LK68
- STRING:   Q1LK68
- GeneID:   4039406
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2581
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- PhylomeDB:   Q1LK68
- ProtClustDB:   PRK05710
- BioCyc:   RMET266264:RMET_2581-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: NA

Molecular weight: Translated: 33954; Mature: 33823

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 44-44 BINDING 183-183 BINDING 201-201 BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRYRGRFAPSPTGPLHLGSLVTALASWLDARAHGGDWLVRIEDIDYPRCVRDADNDIL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCHHHH
RTLDALGLHPDEPPVWQSRREGLYAEALRQLDAAGYLYPCGCTRKEIADSLVHVRERHQT
HHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
LGYPGTCRNGLHGKLPRAWRVRVPDGPAATICFDDRWQGRQCQNLETELGDFVLRRADGL
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
WAYQLAVVVDDAAQGITHIVRGADLLDSTPRQIHLEHLLGLPTPDYLHVPVVVNAVGEKL
EEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCCCCEEHHHHHHHHHHHH
SKQSGAQALDASAPLDALRTAGTHLGIVNQETTVAAWLATATGQWLERLRAVQDLRGTPP
HHHCCCHHHCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSAATGRLGA
CCCCCCCCCC
>Mature Secondary Structure 
SQRYRGRFAPSPTGPLHLGSLVTALASWLDARAHGGDWLVRIEDIDYPRCVRDADNDIL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCHHHH
RTLDALGLHPDEPPVWQSRREGLYAEALRQLDAAGYLYPCGCTRKEIADSLVHVRERHQT
HHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
LGYPGTCRNGLHGKLPRAWRVRVPDGPAATICFDDRWQGRQCQNLETELGDFVLRRADGL
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
WAYQLAVVVDDAAQGITHIVRGADLLDSTPRQIHLEHLLGLPTPDYLHVPVVVNAVGEKL
EEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCCCCEEHHHHHHHHHHHH
SKQSGAQALDASAPLDALRTAGTHLGIVNQETTVAAWLATATGQWLERLRAVQDLRGTPP
HHHCCCHHHCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSAATGRLGA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA