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The map label for this gene is pdxJ [H]

Identifier: 94311351

GI number: 94311351

Start: 2624948

End: 2625730

Strand: Reverse

Name: pdxJ [H]

Synonym: Rmet_2415

Alternate gene names: 94311351

Gene position: 2625730-2624948 (Counterclockwise)

Preceding gene: 94311352

Following gene: 94311350

Centisome position: 66.84

GC content: 64.75

Gene sequence:

>783_bases
ATGATTTTCCACGCGAACCCCGGTGTCATAGACCTCGGGGTCAATATCGACCACGTGGCCACGCTGCGCAACGCGCGCGG
CACGGTGTATCCCGATCCGATCCAGGCAGCGCTGCAGGCCGAGGAGGCCGGCGCCGACCTGATCACGCTGCATCTGCGCG
AGGACCGTCGCCATATCCGCGACGCCGACGTGCGCGCGCTGCGCCCGAAGTTGGCCACGCGCATGAATCTGGAATGTGCG
ATCACTTCCGAGATGCTCGACATTGCGTGCGAGATCAAGCCCCAGGACGTCTGCCTGGTGCCCGAGAAGCGCGAGGAAGT
TACCACCGAAGGCGGACTCGATGTAGCGGGCCATTTCGAGCAAGTCAGCGCCGCATGCCGGCAACTGGCCGACGCGGGTA
TCCGTGTATCGCTGTTCATTGATGCTGACCCTGACCAAATTGCCGCCGCTGCCGCGTGCAAGGCGCCGGTAATTGAAATC
CATACTGGCCGCTACGCCGATGCGCATACGGCGGAGGAGCAGGCAGCCGAGTTCCGCCGTGTAGCGGCGGGCGTGGAAGC
GGGGCAGAAGCATGGTCTGATCGTCAACGCCGGCCACGGCCTGCACTACACCAACGTGCAGCCGATTGCTGCGCTGCCGG
GGATCAAGGAGCTCAACATTGGTCACGCGATCGTTGCCCATGCGGTGTTTGCCGGGTGGCAGAACGCCGTGCGCGAGATG
AAGGCCATCATGGTGGCGGCGCGCCTCGGTACCCGTTACCCGGTTCCGGGTCAGCCCGCGTGA

Upstream 100 bases:

>100_bases
CTGCTGCATTATTATTTGCAGGGTGCGCCACTGAAGACGCGCCAGATCCTGATCGATCTGCAGTACCTTTAATATCCCAA
TTCTCCTGAGAGAAGCCGGC

Downstream 100 bases:

>100_bases
TCTACGGCGTCGGCACCGACATCATCCAGATTGATCGCGTGCGCGGCGTGATGGAGCGCACGCGTGGGCGCTTTGTCGAG
AAGATTCTCGGGCCGCGTGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MIFHANPGVIDLGVNIDHVATLRNARGTVYPDPIQAALQAEEAGADLITLHLREDRRHIRDADVRALRPKLATRMNLECA
ITSEMLDIACEIKPQDVCLVPEKREEVTTEGGLDVAGHFEQVSAACRQLADAGIRVSLFIDADPDQIAAAAACKAPVIEI
HTGRYADAHTAEEQAAEFRRVAAGVEAGQKHGLIVNAGHGLHYTNVQPIAALPGIKELNIGHAIVAHAVFAGWQNAVREM
KAIMVAARLGTRYPVPGQPA

Sequences:

>Translated_260_residues
MIFHANPGVIDLGVNIDHVATLRNARGTVYPDPIQAALQAEEAGADLITLHLREDRRHIRDADVRALRPKLATRMNLECA
ITSEMLDIACEIKPQDVCLVPEKREEVTTEGGLDVAGHFEQVSAACRQLADAGIRVSLFIDADPDQIAAAAACKAPVIEI
HTGRYADAHTAEEQAAEFRRVAAGVEAGQKHGLIVNAGHGLHYTNVQPIAALPGIKELNIGHAIVAHAVFAGWQNAVREM
KAIMVAARLGTRYPVPGQPA
>Mature_260_residues
MIFHANPGVIDLGVNIDHVATLRNARGTVYPDPIQAALQAEEAGADLITLHLREDRRHIRDADVRALRPKLATRMNLECA
ITSEMLDIACEIKPQDVCLVPEKREEVTTEGGLDVAGHFEQVSAACRQLADAGIRVSLFIDADPDQIAAAAACKAPVIEI
HTGRYADAHTAEEQAAEFRRVAAGVEAGQKHGLIVNAGHGLHYTNVQPIAALPGIKELNIGHAIVAHAVFAGWQNAVREM
KAIMVAARLGTRYPVPGQPA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=237, Percent_Identity=66.2447257383966, Blast_Score=304, Evalue=5e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 27905; Mature: 27905

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFHANPGVIDLGVNIDHVATLRNARGTVYPDPIQAALQAEEAGADLITLHLREDRRHIR
CEEECCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHH
DADVRALRPKLATRMNLECAITSEMLDIACEIKPQDVCLVPEKREEVTTEGGLDVAGHFE
CCHHHHHHHHHHHHCCCEEEEHHHHHHEEEECCCCCEEECCCHHHHHCCCCCCCHHHHHH
QVSAACRQLADAGIRVSLFIDADPDQIAAAAACKAPVIEIHTGRYADAHTAEEQAAEFRR
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
VAAGVEAGQKHGLIVNAGHGLHYTNVQPIAALPGIKELNIGHAIVAHAVFAGWQNAVREM
HHHHHHCCCCCCEEEECCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KAIMVAARLGTRYPVPGQPA
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIFHANPGVIDLGVNIDHVATLRNARGTVYPDPIQAALQAEEAGADLITLHLREDRRHIR
CEEECCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHH
DADVRALRPKLATRMNLECAITSEMLDIACEIKPQDVCLVPEKREEVTTEGGLDVAGHFE
CCHHHHHHHHHHHHCCCEEEEHHHHHHEEEECCCCCEEECCCHHHHHCCCCCCCHHHHHH
QVSAACRQLADAGIRVSLFIDADPDQIAAAAACKAPVIEIHTGRYADAHTAEEQAAEFRR
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
VAAGVEAGQKHGLIVNAGHGLHYTNVQPIAALPGIKELNIGHAIVAHAVFAGWQNAVREM
HHHHHHCCCCCCEEEECCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KAIMVAARLGTRYPVPGQPA
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA