Click here to switch to the map view.

The map label for this gene is ispG [H]

Identifier: 94311044

GI number: 94311044

Start: 2287670

End: 2288962

Strand: Reverse

Name: ispG [H]

Synonym: Rmet_2106

Alternate gene names: 94311044

Gene position: 2288962-2287670 (Counterclockwise)

Preceding gene: 94311045

Following gene: 94311043

Centisome position: 58.27

GC content: 63.42

Gene sequence:

>1293_bases
ATGAACCAGAACGCTTTCCAGCCCGTGATCCCGGGCCCGCTGCCGCGTCGCGTTTCGCGCCAGGCCAGTGTTGTGTGGGG
CGACAACATCGTGACGATCGGCGGCGGCGCGCCAGTGCGCGTGCAGTCGATGACCAACACCGATACGGTGGATGCCATCG
GCACCGCAATCCAGATCAAGGAACTGGCACGCGCCGGTTCGGAGATTGTCCGTATCACGGTGAATACGCCGGAAGCCGCC
GCGGCCGTGCCGGCGATTCGCGAACAACTCGACCGTATGGGCGTGGATGTGCCGCTGGTGGGCGACTTCCACTACAACGG
CCATACGCTGCTGCATGACTACCCCGAGTGCGCGCAGGCGCTCTCGAAGTACCGGATCAACCCCGGCAATGTCGGCAAGG
GTGCCAAGCGCGACAAGCAGTTCGCCGAGATGATCGAGATGGCGTGCCGCTACAACAAACCGGTGCGTATCGGCGTGAAC
TGGGGCAGCCTGGATCAGGATTTGCTGGCACGCATCATGGACGAAAACGCCCAGCGCGCCGAGCCGTGGCCCGCGCAAAG
CGTGATGGTAGAGGCGCTGATTACGTCGGCGATCGAGTCGGCCCAGAAGGCTGAAGAGATCGGTTTGCCGGCCAATCAGA
TCATTCTGTCGTGCAAGGTATCGCAGGTGCAGGAACTGGTCGCGGTATACCGCGAGCTGGCGCGCCGCTGCGACTATGCG
CTGCATCTGGGTCTGACTGAAGCCGGCATGGGCAGCAAGGGTATCGTTGCGTCGACGGCTGCGCTATCGGTGCTGCTGCA
GGAAGGTATCGGCGACACGATCCGGATCTCGCTGACGCCGGAACCCGGCGCGCCGCGCGAGAAGGAAGTCTACGTCGGCC
AGGAAATCCTGCAGACGATGGGGCTGCGCAACTTCACGCCGATGGTGATCGCGTGCCCGGGATGCGGTCGCACCACCAGC
ACAACGTTCCAGGAGCTGGCAGCAAGCATCCAGTCGTATCTGCGCGAGCAGATGCCGGTCTGGAAGACGGCCTACCCGGG
CGTGGAAGAAATGGATGTGGCAGTGATGGGCTGCATCGTCAACGGCCCTGGCGAAAGCAAGCACGCCAACATCGGTATTT
CGCTGCCGGGTTCGGGCGAATCGCCTGCGGCGCCGGTGTTCGTGGACGGCGTCAAGGTGAAGACCCTGCGCGGCGAGAAC
ATCGCGCAGGAGTTCCAGGCGATCGTCGACGAGTATGTACGTTCGCACTACGGTCCGGACGCGAAACAGGCTTCACAAGA
AGCGGTGGCCTGA

Upstream 100 bases:

>100_bases
GAACTTCCAGGCGTCCAACCGGAATAATGTGGCCCGCCTGACCCTGCAGTAATGCGCTGCGGGTGGCGCGGCCAGCCATG
CAAGAAAAGGTGGTAATGCA

Downstream 100 bases:

>100_bases
TCGATTCGGCGCCATCGCGCAAGGATCGGGCATCACCGTAACAACAAATCAGACATGAGCGAAGACAAGACGAAGGCCGT
CAAGGCACTGCAGGGCGTGA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNQNAFQPVIPGPLPRRVSRQASVVWGDNIVTIGGGAPVRVQSMTNTDTVDAIGTAIQIKELARAGSEIVRITVNTPEAA
AAVPAIREQLDRMGVDVPLVGDFHYNGHTLLHDYPECAQALSKYRINPGNVGKGAKRDKQFAEMIEMACRYNKPVRIGVN
WGSLDQDLLARIMDENAQRAEPWPAQSVMVEALITSAIESAQKAEEIGLPANQIILSCKVSQVQELVAVYRELARRCDYA
LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTMGLRNFTPMVIACPGCGRTTS
TTFQELAASIQSYLREQMPVWKTAYPGVEEMDVAVMGCIVNGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGEN
IAQEFQAIVDEYVRSHYGPDAKQASQEAVA

Sequences:

>Translated_430_residues
MNQNAFQPVIPGPLPRRVSRQASVVWGDNIVTIGGGAPVRVQSMTNTDTVDAIGTAIQIKELARAGSEIVRITVNTPEAA
AAVPAIREQLDRMGVDVPLVGDFHYNGHTLLHDYPECAQALSKYRINPGNVGKGAKRDKQFAEMIEMACRYNKPVRIGVN
WGSLDQDLLARIMDENAQRAEPWPAQSVMVEALITSAIESAQKAEEIGLPANQIILSCKVSQVQELVAVYRELARRCDYA
LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTMGLRNFTPMVIACPGCGRTTS
TTFQELAASIQSYLREQMPVWKTAYPGVEEMDVAVMGCIVNGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGEN
IAQEFQAIVDEYVRSHYGPDAKQASQEAVA
>Mature_430_residues
MNQNAFQPVIPGPLPRRVSRQASVVWGDNIVTIGGGAPVRVQSMTNTDTVDAIGTAIQIKELARAGSEIVRITVNTPEAA
AAVPAIREQLDRMGVDVPLVGDFHYNGHTLLHDYPECAQALSKYRINPGNVGKGAKRDKQFAEMIEMACRYNKPVRIGVN
WGSLDQDLLARIMDENAQRAEPWPAQSVMVEALITSAIESAQKAEEIGLPANQIILSCKVSQVQELVAVYRELARRCDYA
LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTMGLRNFTPMVIACPGCGRTTS
TTFQELAASIQSYLREQMPVWKTAYPGVEEMDVAVMGCIVNGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGEN
IAQEFQAIVDEYVRSHYGPDAKQASQEAVA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=388, Percent_Identity=35.5670103092784, Blast_Score=201, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46287; Mature: 46287

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNAFQPVIPGPLPRRVSRQASVVWGDNIVTIGGGAPVRVQSMTNTDTVDAIGTAIQIK
CCCCCCCCCCCCCCCHHHCCCCEEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHH
ELARAGSEIVRITVNTPEAAAAVPAIREQLDRMGVDVPLVGDFHYNGHTLLHDYPECAQA
HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHH
LSKYRINPGNVGKGAKRDKQFAEMIEMACRYNKPVRIGVNWGSLDQDLLARIMDENAQRA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCC
EPWPAQSVMVEALITSAIESAQKAEEIGLPANQIILSCKVSQVQELVAVYRELARRCDYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCCE
LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTM
EEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHC
GLRNFTPMVIACPGCGRTTSTTFQELAASIQSYLREQMPVWKTAYPGVEEMDVAVMGCIV
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHCEEEEEEEE
NGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGENIAQEFQAIVDEYVRSHYGPD
CCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
AKQASQEAVA
HHHHHHHCCC
>Mature Secondary Structure
MNQNAFQPVIPGPLPRRVSRQASVVWGDNIVTIGGGAPVRVQSMTNTDTVDAIGTAIQIK
CCCCCCCCCCCCCCCHHHCCCCEEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHH
ELARAGSEIVRITVNTPEAAAAVPAIREQLDRMGVDVPLVGDFHYNGHTLLHDYPECAQA
HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHH
LSKYRINPGNVGKGAKRDKQFAEMIEMACRYNKPVRIGVNWGSLDQDLLARIMDENAQRA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCC
EPWPAQSVMVEALITSAIESAQKAEEIGLPANQIILSCKVSQVQELVAVYRELARRCDYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCCE
LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTM
EEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHC
GLRNFTPMVIACPGCGRTTSTTFQELAASIQSYLREQMPVWKTAYPGVEEMDVAVMGCIV
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHCEEEEEEEE
NGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGENIAQEFQAIVDEYVRSHYGPD
CCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
AKQASQEAVA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]